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Citations for GE Life Sciences :
3101 - 3150 of 4264 citations for Recombinant Human CFHR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... we further purified the proteins using a Superose™ 6 Increase 10/300 GL (GE Healthcare, 29-0915-96) size-exclusion column pre-equilibrated with 50 mM sodium acetate pH 4.2 ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by Ni-NTA immobilized metal-affinity chromatography using His-Trap HP columns (GE Healthcare) followed by size exclusion chromatography ...
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bioRxiv - Biophysics 2021Quote: Protein samples were exchanged into a H-buffer using a PD-10 G-25 media desalting column (GE Healthcare) to induce droplet formation ...
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bioRxiv - Biochemistry 2020Quote: ... The protein samples were applied to a final size exclusion chromatography step using a Superose 6 column (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The protein and ligand were incubated for 10 min and spotted onto Protran BA85 nitrocellulose (0.45 μm, GE Healthcare) with a replicator tool (VP 408FP6S2 ...
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bioRxiv - Molecular Biology 2020Quote: ... All proteins were purified by Ni-NTA affinity chromatography using a 5 ml HisTrap column (GE Healthcare Life Sciences) equilibrated with 50 mM Tris-HCl pH 7.5 and 150 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was further purified with size-exclusion chromatography on HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare) in NMR buffer ...
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bioRxiv - Immunology 2021Quote: The purification of 2H1 from NS0 cells consisted of affinity chromatography using rProtein A/Protein G GraviTrap (GE Healthcare). The antibodies were eluted with a 0.1 M glycine-HCL pH 2.7 solution and immediately neutralized with a 1 M Tris-HCl 1 M NaCl pH 9.0 buffer to maintain their stability in solution ...
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bioRxiv - Microbiology 2021Quote: ... size-exclusion (SEC) column connected to an ÄTKA™ pure fast protein liquid chromatography (FPLC) system (GE Healthcare Inc.). The column was equilibrated with SEC buffer (50 mM Tris ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... 100 μL of protein sample at 1 mg/mL was injected onto an S200 10/300 column (GE Healthcare) equilibrated in PBS ...
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bioRxiv - Immunology 2021Quote: ... IgG from the hybridoma cell line supernatants was purified by affinity chromatography using protein G columns (GE Life Sciences). Purified H5.28 and H5.31 IgG generated from hybridomas was used for EC50 binding measurements ...
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bioRxiv - Neuroscience 2021Quote: ... Protein-antibody complexes were detected on X-ray films following addition of Enhanced Chemiluminescence (ECL) substrate (GE Healthcare, UK). The relative density of the pCREB and CREB bands was quantitated using ImageJ software (NIH ...
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bioRxiv - Biophysics 2020Quote: ... proteins were affinity purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast flow resin (GE Healthcare). Supernatant was two-fold diluted with 1xPBS (pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... for between 1 - 4 days and the protein signal visualized using a Typhoon Trio Variable Mode Imager (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were detected with horseradish peroxidase–conjugated anti-mouse or anti-rabbit antibodies (GE Healthcare Life Sciences, Marlborough, MA).
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bioRxiv - Microbiology 2022Quote: ... IgG1 and IgG2 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: All chromatography steps were performed at 4 °C using the ÄKTAxpress and ÄKTA pure protein purification systems (GE Healthcare). For affinity purification of the ADGRG4 construct ...
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bioRxiv - Bioengineering 2022Quote: ... All proteins were further purified by size-exclusion chromatography using an S75 or a S200 Increase column (GE Healthcare), depending on protein size ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by size exclusion column (SEC) using HiLoad 26/600 Superdex 200 (GE Healthcare, USA) in SEC buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Eluted protein was further purified by size exclusion chromatography (SEC) using Superose 6 Increase 10/300 column (GE Healthcare), pre-equilibrated with the desired buffers.
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bioRxiv - Biophysics 2022Quote: ... Protein was further purified by size exclusion chromatography using a Superose 6 Increase column (10/300 GL, GE Healthcare) equilibrated with 150 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
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bioRxiv - Plant Biology 2022Quote: ... Dialyzed protein was then loaded onto a 5 ml HiTrap Q HP anion exchange column (GE Healthcare Life Sciences) and eluted with a gradient of 0-100% elution buffer (50 mM Tris-HCl (pH 8) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were visualized with the LAS-4000 system (ImageQuantTM LAS-4000, GE Healthcare Life Sciences, Pittsburgh, PA, USA) after a chemiluminescent reaction (Immobilon® Western Chemiluminescent HRP Substrate ...
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bioRxiv - Immunology 2022Quote: ... the protein-containing supernatant was purified by nickel-affinity and size-exclusion chromatography on an AKTAxpress system (GE Healthcare), which consisted of a 1 mL nickel affinity column followed by a Superdex 200 16/60 gel filtration column ...
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bioRxiv - Immunology 2022Quote: ... the protein was further purified by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... The eluted protein was concentrated and purified by gel-filtration chromatography (Superdex 200 10/300 GL, GE Healthcare, USA), using a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... The cleaved protein was collected and further purified using gel filtration (HiLoad 16/60 Superdex pg 200, GE Healthcare) with GF buffer (20 mM Tris-HCl ...
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: ... and then purified through affinity chromatography using a 1 mL HiTrap Protein G HP column (GE Healthcare Life Sciences). Antibodies were eluted from the column using 0.1 M glycine-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated on a 7.5% Bis-Tris gel and blotted onto a 0.45 μm nitrocellulose membrane (GE Healthcare Life Sciences ...
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bioRxiv - Physiology 2023Quote: ... Lipoproteins were fractionated using a Superose 6 10/300 GL fast protein liquid chromatography (FPLC) column (GE healthcare, UK). Fractions (500 μL ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The extracted mononucleosome fraction was incubated for 2 h at 4°C with Protein G Sepharose beads (GE Healthcare), which were preincubated with rabbit polyclonal anti–chicken CENP-A antibody64 ...
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bioRxiv - Molecular Biology 2024Quote: ... HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare) for 2 hours at 4°C in a rotating wheel ...
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bioRxiv - Systems Biology 2024Quote: ... Proteins were resolved by SDS-PAGE and transferred onto nitrocellulose membrane (GE Healthcare, Amersham™ Protan™ 0.2µM NC). After detection of phospho-ERK1/2 by immunostaining with mouse anti-P-p44/42 MAPK XP (Cell Signalling ...
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bioRxiv - Systems Biology 2024Quote: ... Proteins were resolved by SDS-PAGE and transferred onto nitrocellulose membrane (GE Healthcare, Amersham™ Protan™ 0.2µM NC). ADCY bands were immunostained with mouse anti-ADCY5/6 (Santa Cruz ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (1-5 mg/ml) were loaded individually on a Superdex 200 Increase 10/300 column (GE Healthcare), and the elution profiles were obtained using a tandem of sequentially connected UV-Vis Prostar 335 (Varian ...
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bioRxiv - Microbiology 2023Quote: ... The eluant of protein was concentrated and further purified using a Superdex-200 increase 10/300 GL (GE Healthcare) column equilibrated with a buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein extracts were separated in SDS-PAGE gels and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA). Western blotting was performed according to standard protocols ...
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bioRxiv - Molecular Biology 2024Quote: Protein lysates were separated on 10% SDS-PAGE gels and then transferred to PVDF membranes (GE Healthcare Life Science). The membranes were blocked in phosphate-buffered saline with Tween 20 (PBST ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant containing the cleaved protein was removed and applied to a size exclusion (SEC) column (HiLoad_16/60_Superdex75 prep- grade, GE Healthcare) equilibrated with PBS ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was further purified by SEC using a Superose 6 increase 10/300 column (GE Healthcare, USA) pre-equilibrated with running buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: The purified GST-tagged fusion protein WDR522-334 (100 μg) was bound to Glutathione Sepharose 4B (GE Healthcare, 28952360) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... most of the proteins were loaded onto a Hi-trap Q HP anion exchange column (GE Healthcare, cat. 17115401). The final purification step involved using a size exclusion column ...
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bioRxiv - Developmental Biology 2024Quote: ... was used at 1:10,000 and proteins were visualised by enhanced chemiluminescence using the ECL-Plus system (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: Proteins were further purified by size-exclusion chromatography (SEC) using a Superdex 200 10/300 GL column (GE Healthcare) integrated in the NGC system ...
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bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and proteins in the soluble fraction were purified by Ni-affinity column chromatography (HisTrap 5 ml column, GE Healthcare). The His6-tag was removed by thrombin digestion (1 μg of thrombin per mg of TelSi3 protein ...
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bioRxiv - Biochemistry 2024Quote: ... uncleaved protein as well as cleaved GST were removed by applying everything onto 5 ml SP column (GE Healthcare) equilibrated with 20 mM Tris pH 8.0 ...