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Citations for GE Life Sciences :
3151 - 3200 of 4331 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated by isoelectric focusing in the first dimension using a Multiphor II electrophoresis system (GE Healthcare). In the second dimension ...
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bioRxiv - Plant Biology 2024Quote: ... GST-PWT4 and GST proteins were purified and immobilized on Glutathione Sepharose 4B beads (GE Healthcare, 17-0756). The beads were divided equally into five aliquots and incubated with the same amount of His-Rwt4 ...
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bioRxiv - Biochemistry 2024Quote: ... and the flowthrough containing phosphorylated proteins was purified over Hiload 16/600 Superdex 75 pg column (GE Healthcare) preequilibrated with buffer containing 25 mM Tris (pH 7.5) ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing target protein were pooled and incubated over night at 4°C with PreScission protease (GE Healthcare) at a ratio of 1:100 (w/w ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... proteins in resuspension buffer were bound to paramagnetic beads (SeraMag Speed beads, GE Healthcare, CAT#45152105050250, CAT#651521050502) by addition of 160 µl cleanup solution (130 µL ethanol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... proteins in 2% SDS were bound to paramagnetic beads (SeraMag Speed beads, GE Healthcare, CAT#45152105050250, CAT#651521050502) by addition of ethanol to a final concentration of 50% ...
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bioRxiv - Neuroscience 2024Quote: ... The eluted proteins were further purified by size exclusion chromatography performed on a Superdex-200pg column (GE Healthcare) pre-equilibrated in TBS buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2024Quote: ... and 0.1 mM PMSF) and included 80 pmol of GST-fused protein immobilized on GSH beads (GE Healthcare), 1–8 µg of heparin sulfate ...
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bioRxiv - Cell Biology 2024Quote: ... and subsequently loaded onto a HiTrap SP (CEH-37)/Q (POT proteins) HP 5 mL column (GE Healthcare). An appropriate salt gradient was applied using 20 mM HEPES pH 7.5 buffer ...
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bioRxiv - Immunology 2024Quote: ... were used to concentrate the protein before size exclusion polishing with a S75 16/60 column (GE Healthcare) using 50 mM Tris (pH 8) ...
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bioRxiv - Biochemistry 2024Quote: ... All the proteins were further purified by size-exclusion chromatography using Superdex 200 16/60 column (GE Healthcare) equilibrated in buffer containing 25mM Tris-HCl pH8.0 ...
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bioRxiv - Biophysics 2024Quote: ... Further purification of the TTP protein was accomplished using a HiTrap Q HP ion-exchange column (GE Healthcare), with gradient elution with NaCl buffer ...
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bioRxiv - Cell Biology 2024Quote: ... the protein was further purified by size exclusion chromatography using a S75 16/60 HiLoad column (GE Healthcare). Pure protein fractions were pooled together and concentrated to ∼11 mg/mL.
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bioRxiv - Cell Biology 2024Quote: ... The eluted protein was further purified by size-exclusion chromatography using Superdex 200 16/60 column (GE Healthcare) equilibrated in buffer containing 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... Cleaved protein was filtered (0.45 µm) and further purified by size-exclusion chromatography (Superdex S200 26/60; GE Healthcare) in buffer containing 20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with corresponding antibody and chemiluminescence was visualized according to the manufacturer’s instructions (ECL Advanced; GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... and proteins were visualized using the with Image-Quant LAS 4000 biomolecular imager (GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Cell Biology 2020Quote: ... 35S-labeled proteins were separated by SDS-PAGE and detected with an Autoimaging analyzer (Typhoon FLA-9500; GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: The concentrated protein was then applied to a HiPrep 26/60 Sephacryl S-100 column (DEAE FF) (GE healthcare) which was equilibrated with the re-suspension buffer supplemented with 100 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) and the protein concentration was determined using a 2-D Quant kit (GE Healthcare, Piscataway NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The labelled protein was further purified by size-exclusion chromatography using Superdex 200 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES at pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein fraction eluted with 250 mM imidazole was purified further by MonoQ 5/50 GL column (GE Healthcare) at pH 8.0 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The cleaved protein was then concentrated to 2.0 ml final concentration and loaded on the S200 column (GE healthcare). S200 size exclusion chromatography has yielded a single peak around 230 KDa ...
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bioRxiv - Developmental Biology 2020Quote: ... The protein were electrophoresised for 1h at 150V and subsequently transferred onto MtOH activated polyvinylidene fluoride membranes (GE Healthcare). Equal loading was assessed using Ponceau red solution ...
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bioRxiv - Neuroscience 2021Quote: ... The labeled proteins were separated from the free dyes on a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer.
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bioRxiv - Molecular Biology 2020Quote: ... proteins were separated by SDS-PAGE and transferred to AmershamTM ProtranTM 0.45 μm nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were probed with primary antibodies at dilutions recommended by the suppliers ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody-chromatin complexes were purified with Protein A conjugated to sepharose beads (GE Healthcare, 30 µL GE17-5280-01) for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The ion-exchange chromatography was performed for further protein purification with an anion-exchange column (Source 15Q, GE Healthcare). Then peak fractions were collected and stored at -80 °C before using.
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred from polyacrylamide to nitrocellulose membranes (AmershamTM ProtranTM Premium 0.45 µm NC, GE Healthcare Life sciences, 10600003) by electrotransfer (Bio-Rad laboratories Inc.) ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences). The following antibodies were used ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Synthetic Biology 2020Quote: ... the desalted protein-containing fractions were pooled and loaded on a Q sepharose FF column XK16/32 (GE Healthcare). The protein-containing fractions in the flow-through of the column were again pooled ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified using MabSelect Protein A followed by Capto Q according to manufacturer protocols (GE Healthcare, Chicago, IL). Purified antibodies were concentrated and formulated to ≥25 mg/mL in PBS.
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B and the cleaved topo VI collected in the flow through ...
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bioRxiv - Genetics 2021Quote: ... All protein and DNA localization was done with images that had been deconvolved using SoftWoRx (Applied Precision, GE Healthcare).
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bioRxiv - Genetics 2020Quote: ... Samples were separated on 10–12% SDS-PAGE gels and proteins were transferred to Protran nitrocellulose membranes (GE Healthcare), after which membranes were stained with 0.3% w/v Ponceau S in 3% v/v acetic acid and digitized ...
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bioRxiv - Genetics 2021Quote: ... All protein and DNA localization was done with images that had been deconvolved using SoftWoRx (Applied Precision, GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: For NMR structure determination the proteins were buffer exchanged using PD-10 columns (Sephadex G-25 M, GE Healthcare) or via gel filtration chromatography in the final purification step to 20mM MES 6.5 ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were then separated by SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose Amersham Protran membrane (GE Healthcare). Membranes were blocked at room temperature with either 5% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... and further purified using size exclusion chromatography (SEC) on an AKTA pure 25L protein purification system (GE Healthcare, USA) in buffer C (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Further purification of the proteins was achieved using a Superdex 200 increase 10/300 size exclusion column (GE Healthcare) in 50 mM HEPES/NaOH pH7.8 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed using 0.1% TBS-T and proteins were detected using ECL or ECL Select (GE Healthcare, USA) on ChemiDoc XRS+ System (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was additionally purified by ion-exchange chromatography on a “Mono S 5/50 GL” column (GE healthcare) at 0-100% B gradient over 15 column volumes at 0.5 ml/min flow rate using 20 mM sodium phosphate buffer (pH 6.0 at 25 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by size exclusion chromatography using an S75 or S200 16/60 column (GE Healthcare) equilibrated in running buffer (10 mM Tris/HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was applied to size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE healthcare) in gel filtration buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were further purified by gel filtration using a Superdex 200 Increase 10/300 GL column (GE Healthcare). nsp7 and nsp8 were purified in SEC-150 buffer ...
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bioRxiv - Biochemistry 2020Quote: Soluble protein was manually applied at room temperature to a 5 mL HiTrapQ anion exchange column (GE Healthcare # 29051325) that had been pre-equilibrated in buffer B ...
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bioRxiv - Biophysics 2021Quote: ... The protein was further purified by size exclusion chromatography using a Superose 6 column (10/300 GL, GE Healthcare) equilibrated with 200 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified in one step by IMAC followed by size exclusion chromatography using ÅKTAxpress system (GE Healthcare) as previously described with some modifications (28) ...