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Citations for GE Life Sciences :
3051 - 3100 of 4331 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare), and purity was confirmed to be at least 90% by SDS polyacrylamide gel electrophoresis ...
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bioRxiv - Synthetic Biology 2021Quote: ... Concentrated protein was loaded onto a HiLoad 26/60 Superdex 200 prep grade column (GE Healthcare, Piscataway, NJ) that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris ...
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bioRxiv - Synthetic Biology 2021Quote: ... Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (GE Healthcare, Piscataway, NJ) that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated and further purified using an S200 16/60 gel filtration chromatography column (GE Healthcare) previously equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein bands were detected with the enhanced chemiluminescence method (FUJIFILM Wako) and chemiluminescence film (GE Healthcare, Buckinghamshire, UK), and target protein levels were analyzed using NIH ImageJ software.
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bioRxiv - Microbiology 2020Quote: ... protein bands were visualized by chemiluminescence and the image was captured by ImageQuant LAS 4000 mini (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... Protein samples were resolved on the SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare). The membrane was blocked with 5% non-fat dry milk in tris-buffered saline ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Proteins of interest were visualized on a Bio-Rad ChemiDoc MP imager with Enhanced Chemiluminescence Plus (GE Healthcare) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: Cell lysates (10μg protein) were resolved by electrophoresis and transferred to a polyvinylidene fluoride membrane (GE Healthcare, UK). Membranes were blocked for 1 hour in 5% skimmed milk/TBS/0.1% Tween-20 and incubated with primary antibodies (1:1000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Protein was further purified by size exclusion over a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) in 100 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification ...
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bioRxiv - Microbiology 2021Quote: ... The protein extracts were separated by SDS-PAGE and were subsequently transferred to Amersham Protran membranes (GE Healthcare) in a tankblot device (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of proteins were resolved in Tris-acetate SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). For extracellular vesicle fractions ...
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bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superose 6 Increase 3.2/300 column (GE Healthcare) equilibrated at 4°C in 40 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated in 40 mM HEPES pH 7.4 (4 °C) ...
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bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superose 6 Increase 3.2/300 column (GE Healthcare) equilibrated at 4°C in 40 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The excess dye was separated from the labelled protein by SEC (Superdex 75 10/300 GL, GE Healthcare) performed in SEC buffer D (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... cells according to the high titer protocol provided and purified on a Protein A HiTrap column (GE Healthcare) with the ACTA Pure FPLC system (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... the bromodomain was purified further using fast-protein liquid chromatography on an AKTA Purifier UPC 10 (GE Healthcare), equipped with a HiPrep 16/60 Sephacryl S-100 High Resolution column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare, USA) and purified using Assay Buffer or Assay Buffer without EDTA (for use in DNA cleavage assays) ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was further purified using a Superdex Increase 10/300 GL column (GE Healthcare, Chicago, IL) equilibrated with buffer containing 350 mM NaBr ...
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bioRxiv - Cancer Biology 2021Quote: ... We subjected equal amounts of proteins to SDS-PAGE and transferred them to nitrocellulose membranes (GE Healthcare #10401197). Non-specific binding was blocked by incubation with TBST plus 5% non-fat milk ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was loaded on a HiPrep Q FF Anion Exchange Column (GE Healthcare Life Sciences, Marlborough, MA) and eluted by buffer with 300 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... The protein complex was then purified by SEC using a Superdex 200 Increase 10/300 GL (GE Healthcare) column ...
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bioRxiv - Microbiology 2020Quote: ... All mAbs were purified from cell culture supernatant using a HiTrap protein G HP affinity column (GE Healthcare) and stored at −80 °C ...
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bioRxiv - Immunology 2021Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification.
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bioRxiv - Immunology 2020Quote: ... we used fritted deep well plates containing 25 μL of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was further purified by gel filtration using a HiLoad Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... fritted deep-well plates were used containing 25 μl of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Immunology 2021Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatant using a HiTrap protein A column (Cytiva, GE Healthcare). IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva ...
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bioRxiv - Microbiology 2021Quote: Proteins resolved by SDS PAGE were transferred to a nitrocellulose transfer membrane (GE Healthcare Amersham™ Protran™) at 80 V for 75 minutes or 15 V overnight at 4°C using a Bio-Rad wet transfer apparatus ...
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bioRxiv - Immunology 2021Quote: ... The protein was further purified on a Superdex 200 Increase 10/300 GL size exclusion column (GE Healthcare). SDS-PAGE was performed to determine the purity and size of the protein ...
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bioRxiv - Cell Biology 2021Quote: Approximately 5 μg of GST or GST-tagged protein fragments were incubated in Glutathione-Sepharose beads (GE Healthcare) in GST-lysis buffer at 4°C overnight ...
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bioRxiv - Plant Biology 2020Quote: Thylakoid proteins were separated by SDS-PAGE and then transferred to a polyvinylidene membrane (Hybond P, GE Healthcare) using a tank blot system (Perfect Blue Web M ...
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bioRxiv - Cell Biology 2021Quote: ... protein extracts were subject to SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL). Primary antibodies used were anti-P2Y1 (Santa Cruz ...
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bioRxiv - Microbiology 2022Quote: ... Strep-tagged proteins were affinity-purified on an ÄKTA Purifier using 5 ml StrepTrap HP columns (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Salt concentration is adjusted and protein is loaded on a 10 ml HiTrap Heparin HP column (GE Healthcare) equilibrated in 20 mM HEPES- KOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... All target proteins were subjected to size exclusion chromatography using the HiLoad 16/600 Superdex 75 (GE Healthcare) equilibrated in 25 mM Tris pH8.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... After protein fractionation by SDS-page samples were transferred to a nitrocellulose membrane (cat#10600002 from GE healthcare) and incubated with primary antibody solutions overnight at 4 °C (p21 ...
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bioRxiv - Immunology 2022Quote: The total IgG was purified from murine serum using Protein G Sepharose 4 Fast Flow columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein was loaded and separated in SDS-page gels and then transferred to PVDF membrane (GE Healthcare). For blocking ...
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bioRxiv - Plant Biology 2022Quote: ... Protein was concentrated using a spin concentrator (Amicon, 10K MWCO) and injected onto Superdex 200 column (GE Healthcare) equilibrated in 25 mM HEPES (pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... protein samples were run on 12-15% polyacrylamide gels and transferred to nitrocellulose Hybond™-C (GE Healthcare) membranes via Bio-Rad Trans-blot Turbo transfer machine ...
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bioRxiv - Microbiology 2022Quote: ... the protein was concentrated and loaded onto a Superdex 75 16/60 size exclusion (SEC) column (GE Healthcare). The peak corresponding to the protein was concentrated to about 12 mg/ml and flash frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Microbiology 2022Quote: ... The supernatants containing antibodies were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... The protein solutions were further diluted with buffer B and eIF4A1 fractions subjected to a ResourceQ (GE Healthcare) anion exchange column ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted proteins were concentrated and applied onto a Superdex 75 HiLoad 26/60 size-exclusion column (GE Healthcare) pre-equilibrated with gel-filtration buffer (10 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... Molecular weights were determined using pre-stained protein ladders (Thermo Fisher Scientific, catalog #26616; GE Healthcare, catalog #RPN800E). Protein abundance was detected on membranes using the SuperSignal West Femto enhanced chemiluminescence kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Immunoprecipitation was performed by incubating sheared chromatin with Protein G Sepharose™ 4 Fast Flow beads (GE Healthcare) and antibodies ...
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bioRxiv - Immunology 2022Quote: ... the supernatant was diluted to a total protein concentration of 100 μg/ml in binding buffer (GE Healthcare). Following purification ...