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Citations for GE Life Sciences :
3051 - 3100 of 4264 citations for Recombinant Human DKK3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Expressed CYP168A1 was purified using fast protein liquid chromatography with a HisTrap-HP affinity column (GE Healthcare, Chicago, IL). The bacterial cell pellets were thawed on ice and resuspended in buffer A consisting of 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... all proteins were further purified using size-exclusion chromatography on a Superdex-200 column (GE Healthcare, Buckinghamshire, United Kingdom). Their corresponding elution profiles are shown in Figure S12 ...
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bioRxiv - Bioengineering 2020Quote: The eluted proteins were concentrated and subjected to size-exclusion chromatography (Superose 6 Increase 10/300 GL, GE Healthcare) in the PBS buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were typically resolved on 7.5% TGX stain-free gels and transferred onto nitrocellulose membrane (GE Healthcare, Amersham Protran) using wet-transfer at 100 V for 60 min ...
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bioRxiv - Microbiology 2022Quote: ... and for HLA-I IP 25 μl Protein G Sepharose® 4 Fast Flow (GE Healthcare, GE17-0618-01) were employed with the HC10 antibody for HLA-IP overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Protein fractions were pooled for buffer exchange employing a PD-10 Sephadex™ G-25 desalting column (GE Healthcare) and centrifugal filters (Amicon®Ultracel®-10 K/30 K ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a 7.5% Bis-Tris gel and blotted onto a 0.45 µm nitrocellulose membrane (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... the protein was applied to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg, GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were first purified with Ni Agarose 6 FF (AOGMA) with the AKTA system (GE Healthcare Life Sciences) and further purified with anti-Flag affinity beads ...
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bioRxiv - Plant Biology 2019Quote: ... pastoris and the peptide was purified from the culture medium using the Fast Protein Liquid Chromatography System (GE Healthcare) and Reverse Phase-High Performance Liquid Chromatography (RP-HPLC ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was dialyzed against storage buffer and applied to a HiTrap Q HP anion exchange column (GE Life sciences) pre-equilibrated in buffer (50 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Biochemistry 2019Quote: RAD51 purification was carried out by chromatography at 4°C using the AKTA Pure Protein Purification System (GE Healthcare). For WT RAD51 ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Microbiology 2019Quote: ... The cleaved protein was concentrated and loaded onto a Superdex 75 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 50 mM Hepes pH8 ...
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bioRxiv - Biophysics 2019Quote: ... The K48-diUb protein was purified from the reaction mixture using Superdex 75 and Source-S columns (GE Healthcare) in tandem ...
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bioRxiv - Biophysics 2019Quote: ... We selected four proteins as molecular mass standards from the gel filtration calibration kit HMW (GE Healthcare, Uppsala, Sweden), i.e ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were further purified by size-exclusion chromatography on a Superdex 200 increase 10/300 GL column (GE Healthcare), equilibrated in 20 mM sodium citrate pH 5.0 ...
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bioRxiv - Biochemistry 2019Quote: ... Diluted protein samples in buffer C were loaded on a heparin HP 5 mL column (GE Healthcare, Chicago, IL) and protein was eluted with buffer D (25mM Tris pH 7.6 ...
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bioRxiv - Immunology 2019Quote: Collected mouse serum was pooled and IgG purified using protein G Ab SpinTraps (GE Healthcare Cat.# 28-4083-47) then concentrated and buffer-exchanged into PBS using Amicon Ultra 30K centrifugal filter units (Merck Millipore Cat.# UFC503024 ...
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bioRxiv - Biochemistry 2019Quote: ... The size-exclusion column was calibrated using a set of standard proteins (Gel Filtration HMW Calibration Kit, GE Healthcare) in SEC buffer ...
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bioRxiv - Biophysics 2019Quote: ... Clarified cell lysate containing His6-tagged protein was loaded onto a Ni–Sepharose affinity column (HisTrap HP; GE Healthcare). The column was washed with wash buffer (5% glycerol ...
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bioRxiv - Biophysics 2019Quote: ... MonoQ fractions containing the protein were concentrated before application to a HiLoad 26/600 Superdex 200 pg (GE Healthcare) equilibrated in PBS pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... protein extracts were resolved by 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (#10600021, GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... The untagged proteins were further purified and concentrated using 1 mL HiTrapTM Heparin HP columns (GE Healthcare Life Sciences). The eluted proteins were analyzed on 10% SDS-PAGE gels and pure fractions (>95% purity ...
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bioRxiv - Plant Biology 2020Quote: ... The extracts were incubated with 5 μL anti-HA antibodies coupled with 30 μL Protein-A Sepharose (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 spike protein in dPBS was applied to a 1-ml HiTrap heparin-Sepharose column (GE Healthcare). The column was washed with 5 ml of dPBS and bound protein was eluted with a gradient of NaCl from 150 mM to 1 M in dPBS.
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bioRxiv - Cell Biology 2020Quote: ... The eluted proteins were further purified by gel filtration using Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with buffer containing 25 mM Tris-Cl pH 7.6 ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative analyses and the relative-fold changes for protein bands were computed using ImageQuant-TL 7.0 software (GE Healthcare). Membranes were stained using the MemCode™reversible stain according to the manufacturer’s instructions (Pierce ...
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bioRxiv - Synthetic Biology 2021Quote: ... Excess DNA was removed from conjugated proteins using size exclusion chromatography on a Superdex200 10/300 column (GE Healthcare) in a 25 mM Tris buffer (pH 8 ...
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bioRxiv - Biophysics 2020Quote: ... The protein was purified from the supernatant of the lysate using a 5 ml HisTrap HP column (GE Healthcare). The Smt3 tag was cleaved off before further purification by a HiLoad 16/600 Superdex 75 size exclusion column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein patterns in the polyacrylamide gels were recorded as digitized images using a calibrated densitometric scanner (GE Healthcare, USA) and analyzed (normalization ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted proteins were further purified using a size-exclusion column (Superose 6 Increase 10/300 GL, GE healthcare) equilibrated with buffer B (20 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... The protein was then further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 200 (GE Healthcare) column using buffer III and a flowrate of 1 mL/min ...
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bioRxiv - Biochemistry 2021Quote: ... the M-Lipxtal protein was further purified by SEC using a HiLoad Superdex 75 26/600 column (GE Healthcare) equilibrated with SEC Buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The protein was further purified by size-exclusion chromatography on a Superdex 75 10/300 column (GE Healthcare, USA) equilibrated in buffer GF [20 mM Tris pH 7.0 ...
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bioRxiv - Genetics 2020Quote: ... Plasma lipoprotein particles were size fractionated by Fast Protein Liquid Chromatography (FPLC) using a Superose 6 column (GE Healthcare) and the cholesterol content in each fraction was measured accordingly26.
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bioRxiv - Neuroscience 2020Quote: ... Visualization of proteins was done by using Amersham ECL Western Blotting Detection Reagent and Amersham Hyperfilm (both GE Healthcare). Densitometric analysis of proteins was performed by ImageJ software ...
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bioRxiv - Cancer Biology 2019Quote: ... The concentrated protein was further purified using HiLoad 16/600 Superdex-75 prep-grade gel-filtration column (GE Healthcare) pre-equilibrated with 20mM Tris HCl [pH 7.5] ...
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bioRxiv - Biochemistry 2020Quote: ... Twenty to 30 μg of proteins were separated by SDS-PAGE and then transferred to nitrocellulose membranes (GE Healthcare). Monoclonal anti-glutathione peroxidase 1 (GPx1 ...
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bioRxiv - Plant Biology 2019Quote: ... Samples containing 100 µg of protein were injected into a Superdex 200 increase 300/10 GL column (GE Healthcare) using a HPLC system (Ultimate 3000 ...
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bioRxiv - Biophysics 2019Quote: ... The eluted proteins from Ni-NTA column were loaded onto a Hi-load 16/60 Superdex 200 (GE Healthcare) gel filtration column pre-equilibrated with 25mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: Proteins were purified by size exclusion chromatography using Superdex200 10/300 GL column attached to AKTA purifier (GE Healthcare). Analytical gel filtration experiments were done using SHODEX Protein KW-800 GFC column (Showa-Denkko-KK ...
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bioRxiv - Immunology 2021Quote: ... The protein bands were detected using the enhanced chemiluminescence (ECL) Prime Western blotting detection system (GE HealthCare, Piscataway, NJ). The images of the blot were acquired on the ChemiDoc XRS using Bio-Rad ImageLab software (Bio-Rad laboratories ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The protein was purified from the clarified supernatant using a Ni-NTA affinity chromatography column (HisTrap FF GE Healthcare) with binding buffer and eluted through on column cleavage with HRV 3C protease ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1h at 4°C followed by incubation with protein A-sepharose beads (GE Healthcare #GE17-0780-01) for 2h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The concentration of the protein was estimated from the absorbance at 280 nm measured by a SimpliNano (GE healthcare) microvolume spectrophotometer with the extinction coefficient calculated by using the ExPASy ProtParam tool (51 ...
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bioRxiv - Physiology 2021Quote: ... The protein bands were detected using a chemiluminescence detection system (ImageQuant LAS 4000 mini; GE Healthcare, Little Chalfont, UK).
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bioRxiv - Microbiology 2021Quote: ... The supernatant was harvested on day 5 and ACE2 protein was purified using HisTrap FF column (GE Life Sciences), followed by desalting ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the dye-labelled protein were detected by in-gel fluorescence imaging with an Amersham Typhoon (GE Healthcare) laser scanner.