Labshake search
Citations for GE Life Sciences :
2901 - 2950 of 4264 citations for Recombinant Human DKK3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to anion exchange chromatography (MonoS ...
-
bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS ...
-
bioRxiv - Microbiology 2023Quote: ... proteins were separated by immobilized-metal affinity chromatography (IMAC) on a 5 mL HisTrap HP column (GE Healthcare), previously washed with ddH2O and equilibrated with buffer B (25 mM HEPES/NaOH pH 7.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated with mouse monoclonal anti-MYC-antibody (Covance) and protein G-agarose beads (GE Healthcare). The precipitates were then washed with a lysis buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... The concentrated protein sample was loaded on a pre-equilibrated Superdex-75 16/60 column (GE Healthcare, USA). A buffer consisting of 50 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein was further purified by size exclusion chromatography (SEC) (Superdex 200 Increase 10/300 GL, GE Healthcare) in 20 mM Tris pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Fractions containing purified proteins were concentrated and further purified by a Superdex 200 size-exclusion column (GE Healthcare) equilibrated in buffer containing in the 25 mM Tris pH 7.5 ...
-
bioRxiv - Bioengineering 2023Quote: ... The ErCas12a protein in clarified lysate was sequentially purified using immobilized metal affinity chromatography (HisTrap HP, GE Healthcare) and heparin chromatography (HiTrap Heparin HP ...
-
bioRxiv - Biochemistry 2023Quote: ... 200 µL of protein for analytical runs) was injected into the Superdex 75 size exclusion column (GE Healthcare) (volume less than 900 µL) ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein was further purified over a size exclusion chromatography column (Hiload 16/60 Superdex75 column, GE Healthcare) equilibrated with buffer containing 20 mM MES pH 6.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 10 mM glutathione and further purified on a Superdex 200 10/300 (GE Healthcare) column in 50 mM Hepes pH 7.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... The protein was then loaded onto a HiLoad Superdex 75 16/60 size exclusion chromatography column (GE Healthcare) that had been pre-equilibrated in Hepes 30 mM ...
-
bioRxiv - Biophysics 2023Quote: ... Protein was purified using a 20 mL NiNTA column (Cytiva) attached to an Akta M FPLC (GE Healthcare) and with a 300 mM imidazole elution buffer ...
-
bioRxiv - Microbiology 2023Quote: ... The immunocomplex was captured by using protein G Sepharose 4 fast flow beads (17-0618-01/ GE Healthcare). The immunoprecipitated complex was washed two times with IP lysis buffer and one time with mili-Q ...
-
bioRxiv - Biochemistry 2023Quote: ... The GST fusion protein was purified by affinity chromatography on glutathione-Sepharose (GE Healthcare Life Sciences (now Cytiva)) in 300 mM NaCl ...
-
Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulationbioRxiv - Biochemistry 2023Quote: ... The spin-labeled proteins were further purified by HiLoad 26/600 Superdex 200 pg column (GE life sciences) in a buffer of 150mM NaCl ...
-
bioRxiv - Plant Biology 2023Quote: ... Protein containing fractions were pooled and buffer exchange was carried out using a PD-10 column (GE Healthcare) using TES-KCl buffer (25 mM TES/KOH pH 7.5 ...
-
bioRxiv - Physiology 2022Quote: ... The specific proteins were detected with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000 dilution; CtNo_NA934, GE Healthcare, 1:5000 dilution ...
-
bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS/PAGE in 10% gel before wet transfer to 0.45μm nitrocellulose membranes (GE Healthcare) and blocked in 5% dry milk powder in TBS-T (100mM Tris ...
-
bioRxiv - Biochemistry 2023Quote: ... Aurora A protein was thawed and buffer exchanged in the experimental buffer using NAP-5 Columns (GE Healthcare). Experiments typically involved titrating 25 µM of protein in the sample cell with 300 µM of compound in the syringe ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein eluate was then applied to an ion exchange chromatography column (2x 1ml HiTrap Q HP, GE Healthcare) equilibrated with QA buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to a nitrocellulose membrane with a 0.2 μm pore size (GE Healthcare Life Sciences, 10600001) by wet transfer at 100 V for 120 minutes ...
-
bioRxiv - Plant Biology 2023Quote: Thylakoid proteins were separated by SDS-PAGE and then transferred to a polyvinylidene membrane (Hybond P, GE Healthcare) using a tank blot system (Perfect Blue Web M ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... then stored at −20°C before purification by fast protein liquid chromatography (FPLC) (AKTA Purifier; GE Healthcare, UK) using a pre-packed HiLoad Superdex 30 26/600 column equipped with a UV detector ...
-
bioRxiv - Plant Biology 2024Quote: ... the proteins were subjected to size exclusion chromatography on a Superdex 200 10/300 increase column (GE Healthcare) and eluted using 150 mM Tris–HCl pH 8.8 containing 300 mM sodium chloride ...
-
bioRxiv - Plant Biology 2024Quote: ... the eluted proteins were purified on a HiLoad16/60 Superdex 200 prep-grade gel filtration column (GE Healthcare) using a buffer containing 20 mM Tris (pH 7.5) ...
-
bioRxiv - Immunology 2024Quote: ... GP38 proteins were further purified via SEC using a Superdex 200 Increase 10/300 GL (GE Healthcare Biosciences) in 2 mM Tris pH 8.0 ...
-
bioRxiv - Immunology 2024Quote: ... The concentrated protein was injected into a Superdex 200 Increase 10/300 GL (GE Healthcare 28-9909-44) size exclusion column chromatography system (Waters) ...
-
bioRxiv - Immunology 2024Quote: ... Proteins were separated on 12% SDS polyacrylamide gels and transferred to nitrocellulose membrane (Amersham Hybond-ECL, GE Healthcare). Membranes were blocked in 5% dried milk/TBS-T buffer and incubated over night with anti-mouse TREX1 antibody (clone C-11 ...
-
bioRxiv - Biochemistry 2024Quote: ... and the flowthrough containing phosphorylated proteins was purified over Hiload 16/600 Superdex 75 pg column (GE Healthcare) preequilibrated with buffer containing 25 mM Tris (pH 7.5) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The protein solutions was loaded on to Superose 6 Increase 10/300 GL column (GE Healthcare, Madison, WI) that was equilibrated with a buffer containing 20 mM Tris-HCl [pH 7.5] ...
-
bioRxiv - Biophysics 2024Quote: ... Protein was concentrated and inject into size-exclusion chromatography on a Superdex 16/60 75 column (GE Healthcare) pre-equilibrated in buffer 20 mM Bis-Tris pH 7.0 ...
-
bioRxiv - Biochemistry 2024Quote: ... the protease was removed from (un)cleaved target protein using a HisTrap FF column (GE Healthcare Life Sciences). Remaining nucleic acids were removed using anion exchange chromatography (AIEX ...
-
bioRxiv - Biochemistry 2024Quote: ... TEV protease and non-cleaved protein was removed by IMAC (HisTrap FF Crude column, 5 ml, GE Healthcare) with a linear gradient of imidazole (0-500 mM) ...
-
Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing target protein were pooled and incubated over night at 4°C with PreScission protease (GE Healthcare) at a ratio of 1:100 (w/w ...
-
bioRxiv - Biochemistry 2024Quote: ... Elution fractions containing the most protein were combined and loaded directly onto a size-exclusion column (GE Healthcare) pre-equilibrated with lysis buffer without prior concentration or dialysis of the protein ...
-
bioRxiv - Biochemistry 2024Quote: Proteins were purified by immobilized metal-affinity chromatography (IMAC) on a HisTrap HP nickel-Sepharose column (GE Healthcare) at 4°C following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... thawed protein samples were purified by size-exclusion chromatography using a Superdex 200 (16/600) column (GE Healthcare) in equilibrated in a buffer containing 20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein bands were visualized and imaged by using an ImageQuant LAS 500 scanner (GE Healthcare, Chicago, IL, USA). After transfer ...
-
bioRxiv - Microbiology 2024Quote: ... All protein lysates were separated on 10% polyacrylamide gels and blotted on polyvinyl difluoride membranes (PVDF, GE Healthcare). Blotted membranes were blocked with either 5% non-fat milk or 5% BSA ...
-
bioRxiv - Microbiology 2020Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
-
bioRxiv - Biochemistry 2020Quote: ... Cleaved protein was filtered (0.45 µm) and further purified by size-exclusion chromatography (Superdex S200 26/60; GE Healthcare) in buffer containing 20 mM Tris pH 7.5 ...
-
bioRxiv - Biophysics 2019Quote: ... 1 mM DTT at pH 7.0) while incubating with PreScission protease (protease to protein ratio 1:2000) (GE Healthcare) overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with corresponding antibody and chemiluminescence was visualized according to the manufacturer’s instructions (ECL Advanced; GE Healthcare).
-
bioRxiv - Cell Biology 2020Quote: ... and proteins were visualized using the with Image-Quant LAS 4000 biomolecular imager (GE Healthcare Life Sciences, Pittsburgh, PA).
-
bioRxiv - Cell Biology 2020Quote: ... 35S-labeled proteins were separated by SDS-PAGE and detected with an Autoimaging analyzer (Typhoon FLA-9500; GE Healthcare).
-
bioRxiv - Cell Biology 2020Quote: The concentrated protein was then applied to a HiPrep 26/60 Sephacryl S-100 column (DEAE FF) (GE healthcare) which was equilibrated with the re-suspension buffer supplemented with 100 mM NaCl ...
-
bioRxiv - Cell Biology 2020Quote: ... pH 8.0) and the protein concentration was determined using a 2-D Quant kit (GE Healthcare, Piscataway NJ, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... The labelled protein was further purified by size-exclusion chromatography using Superdex 200 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES at pH 7.5 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The protein fraction eluted with 250 mM imidazole was purified further by MonoQ 5/50 GL column (GE Healthcare) at pH 8.0 ...