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Citations for GE Life Sciences :
3001 - 3050 of 4723 citations for Recombinant Human FCGR3B Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... the reaction was added to 15 µL (30 µL 50% slurry) Protein A:Protein G beads (GE Healthcare) pre-coupled with antibodies ...
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bioRxiv - Microbiology 2019Quote: Fifty micrograms of protein extract from each sample were labeled with Cy3- or Cy5 Dye (GE Healthcare). Labeling was performed reciprocally so that each sample was separately labeled with Cy3 and Cy5 to account for any preferential protein labeling by the CyDyes ...
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bioRxiv - Microbiology 2019Quote: Isolated proteins for each fraction and genotype were then precipitated with 2D Clean-Up kit (GE Healthcare), and dissolved in TS buffer (7M urea ...
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bioRxiv - Biochemistry 2019Quote: ... and the cleaved protein was further purified using FPLC first with cation exchange (Source-S, GE Healthcare), followed by gel filtration (Superdex 75 ...
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bioRxiv - Zoology 2019Quote: ... Wet-transfer of the proteins from the gel to a polyvinylidene difluoride transfer membrane (GE Healthcare, UK) was according to [40] with minor modifications ...
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bioRxiv - Biochemistry 2019Quote: Protein samples were injected onto a Superdex S200 or S75 16/60 size exclusion column (GE Healthcare), which was run at 1 ml.min-1 in 25 mM HEPES pH 7.0 ...
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bioRxiv - Genomics 2020Quote: ... Eluted protein samples were further purified by gel filtration using Superose 6 10/300 column (GE Healthcare). These proteins were buffer-exchanged to storage buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by gel filtration chromatography on Superdex 75 16/600 column (GE Healthcare) in 50 mM Sodium Phosphate ...
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bioRxiv - Microbiology 2019Quote: ... The protein was purified from the soluble fraction using HisTrap HP and Superdex 75 columns (GE Healthcare) in 50 mM HEPES (pH 7.4) ...
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bioRxiv - Immunology 2019Quote: ... followed by the addition of 30 μl of a 50% slurry of protein G beads (GE Healthcare), and incubation overnight at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted proteins were pooled and concentrated for size exclusion chromatography (16/600 Superdex 75pg GE healthcare). The Tau concentration was measured using BCA method.
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the purified protein were pooled and desalted with PD midi Trap G-25 (GE Healthcare) using the spin protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Protein solution was loaded onto a HisTrap HP column connected to a GSTtrap FF column (GE Healthcare) equilibrated in buffer A to remove the GST-tagged PreScission protease ...
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bioRxiv - Bioengineering 2021Quote: ... In vitro translated proteins were visualized by autoradiography using Typhoon FLA 9500 Phosphorimager (GE Healthcare Life Sciences). Signal intensity (corrected for background signal ...
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bioRxiv - Biophysics 2020Quote: ... Protein samples were freshly prepared using size exclusion chromatography (GE Life Sciences, Superdex 75 10/300 GL) in a buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... Proteins of interest were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (GE Healthcare). Peroxidase substrate was provided by ECL Western Blotting Substrate kit (Pierce) ...
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bioRxiv - Immunology 2021Quote: ... Proteins were concentrated in centrifugal concentrators and purified by size-exclusion chromatography (Superdex 200; GE Life Sciences) in gel filtration buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... Proteins were purified as above using Ni2+ affinity and size exclusion chromatography (Superdex 75, GE Life Sciences), excluding cleavage of the His6-tags.
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bioRxiv - Immunology 2021Quote: ... The dialyzed protein was further purified on a pre-equilibrated Superdex 75 gel filtration column (GE Healthcare), and fractions containing monomeric soluble NLRP3PYD were concentrated to 1 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... and loaded on superdex 75 column on an AKTA pure 25L protein purification system (GE Healthcare, USA) in 20 mM HEPES pH 7.5 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... the proteins were further purified using a Hiload 16/600 Superdex 200 pg gelfiltration column (GE Healthcare) in SEC Buffer (20 mM HEPES/NaOH pH7.8 and 250 mM NaCl) ...
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bioRxiv - Biophysics 2021Quote: ... The protein purification was carried out using a cation exchange HiTrap SP column (GE Healthcare, Chicago, IL) with phosphate buffer (25 mM sodium phosphate ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further purified by size-exclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare) column.
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bioRxiv - Biochemistry 2021Quote: ... The protein sample (50 μL) was loaded into a 10/300 Superdex® 200 GL (GE Healthcare) column connected to the MALS detection unit for SEC ...
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bioRxiv - Biochemistry 2020Quote: ... Concentrated protein was then applied to a size exclusion column (HiLoad 16/60 Superdex 200, GE Healthcare) pre-equilibrated with Buffer GF (50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The protein complex eluted as a monodisperse peak from a Superdex 200 gel filtration column (GE Healthcare) in 20 mM HEPES/NaOH pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... All proteins were further purified using Superdex 75 16/60 or Superdex 200 16/60 (GE Healthcare) gel filtration chromatography and stored in their respective running buffers ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated and run over a HiLoad 16/600 superdex 75 gel filtration column (GE Healthcare) using an AKTA pure (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... 0.3 M NaCl using a Fast Protein Liquid Chromatography (FPLC) system (ÄKTA Purifier, GE Healthcare Life Sciences). Prior to loading ...
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bioRxiv - Neuroscience 2019Quote: ... Eluted protein was concentrated and further purified on a Superose 6 10/300 Increase column (GE Healthcare) with 150 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... The proteins were further purified using 16/600 Superdex 75 pg size-exclusion chromatography columns (GE Healthcare) equilibrated in protein buffer (20mM Tris pH 7.8 ...
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bioRxiv - Neuroscience 2020Quote: ... Immune complexes were isolated by the addition of 20 μL of protein G– Sepharose beads (GE Healthcare) followed by incubation for 2 hr at 4°C ...
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bioRxiv - Biophysics 2021Quote: Purified protein or cell lysates were injected onto a Superose 6 increase 5/150 column (GE Healthcare), running in a buffer containing 20 mM Tris (pH 7.5) ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted protein was further purified by size exclusion chromatography using a Superdex 200 prepgrade column (GE Healthcare) in 50 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2021Quote: ... The reconstituted protein was further purified on the Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in a buffer comprising 20 mM Bis-Tris pH 6.8 ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing protein of interest were collected and desalted using PD-10 midi desalting columns (GE Healthcare). Protein of interest was eluted with the storage buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using SDS-PAGE and blotted onto Amersham Protran 0.45 µm nitrocellulose membrane (GE Healthcare). The primary antibodies and dilutions used were ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using SDS-PAGE and blotted onto Amersham Protran 0.45 µm nitrocellulose membrane (GE Healthcare). The primary antibody and dilution used was rat anti-HA-HRP 3F10 (Roche Applied Science) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using SDS-PAGE and blotted onto Amersham Protran 0.45 µm nitrocellulose membrane (GE Healthcare). The primary antibodies and dilutions were ...
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bioRxiv - Cell Biology 2021Quote: ... Labeled proteins were purified by final buffer exchange using size exclusion chromatography (Superdex 200 column, GE Healthcare) in the appropriate buffer (i.e ...
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bioRxiv - Microbiology 2020Quote: ... The concentrated DnaA protein was subjected to size exclusion chromatography on an Äkta pure instrument (GE Healthcare). 200 μL of concentrated DnaA-6xHis was applied to a Superdex 200 Increase 10/30 column (GE Healthcare ...
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bioRxiv - Systems Biology 2021Quote: ... The proteins were detected by Enhanced Chemiluminescence (ECL) using ECL Western Blotting reagents (Amersham, GE Healthcare, UK) or SuperSignal West Dura Extended Duration Substrate (Pierce ...
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was desalted against 1 x PBS buffer and digested with thrombin protease (GE Healthcare) in a ratio of 1 unit per μg at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... denatured proteins were then separated via SDS-PAGE and transferred to a PVDF membrane (GE Healthcare, Amersham). Blots were preincubated in a blocking solution of 5% BSA in 0.2% TBST (0.1 M Tris base ...
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bioRxiv - Plant Biology 2020Quote: ... the purified RCA protein was immobilized on a carboxy-methylated dextran sensor chip (CM5) chip (GE Healthcare) using an amine coupling method kit (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin samples derived from 1.0×106 cells were precleared with protein G sepharose (GE Healthcare, Chicago, IL) and then 1-5 μg of each antibody was added to form immune complex ...
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bioRxiv - Microbiology 2020Quote: ... supernatant containing VSG protein was loaded onto an anion-exchange column (Q-Sepharose Fast-Flow, GE Healthcare), which had been equilibrated with 10 mM phosphate buffer ...
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bioRxiv - Microbiology 2019Quote: ... Typically 100 µg of protein samples were injected into Superdex 200 10/300 increase column (GE Healthcare) by a Shimadzu autosampler coupled HPLC machine ...
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bioRxiv - Cell Biology 2021Quote: ... between 600-900 μg of each CLASP1 protein was injected into a Superose 6 column (GE Healthcare) in a buffer containing 50 mM phosphate pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... The next morning the cleaved protein was loaded on a 5 ml HiTrap HeparinHP column (GE Healthcare). The protein was eluted over a gradient from 100 mM to 2M KCl ...