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Citations for GE Life Sciences :
3251 - 3300 of 4723 citations for Recombinant Human FCGR3B Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Immunology 2021Quote: ... The protein was further purified by size exclusion chromatography using Superdex 200 10/300 GL column (GE Healthcare) equilibrated with 20 mM HEPES at pH 7.4 ...
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bioRxiv - Genomics 2020Quote: ... The signal of resolved protein was visualized by Amersham enhanced chemiluminescence (ECL) Western Blotting Analysis System (GE Healthcare) using Amersham Hyperfilm ECL (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Molecular Biology 2021Quote: ... the immobilized proteins were eluted and further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were assessed by SDS–PAGE followed by Coomassie blue staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Msh5 ChIP was performed using polyclonal Msh5 antibody (generated in rabbit) and Protein A Sepharose beads (GE Healthcare) on synchronized meiotic cultures as described in Krishnaprasad et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The concentrated proteins were then subjected to size-exclusion chromatography using a Superdex-75 GL column (GE healthcare pre-equilibrated with 25 mM Tris-HCl 6.8 ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants containing the protein extracts were then applied onto a HisTrap FF 5 ml column (GE Healthcare) loaded with nickel ions using an ÄKTA Start liquid chromatography machine (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted out and further purified by a mono Q 5/5 column (GE Healthcare) and a Superose S6 Increase 5/150 GL column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... the proteins were diluted to contain 25 mM NaCl and further purified on Resource Q column (GE Healthcare). Size exclusion chromatography on a HiLoad Superdex 75 16/600 column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... fritted deep-well plates were used containing 25 μL of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Microbiology 2020Quote: ... The protein was repurified on Co-NTA agarose followed by gel filtration chromatography on Superdex 200 (GE Healthcare) in buffer A to remove the protease ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples (20 µg) were separated on 15% SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare). Membranes were blocked overnight at 4°C with 5% milk in TBS-T (20 mM Tris pH 7.6 ...
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bioRxiv - Biophysics 2020Quote: ... The eluted protein sample was concentrated and applied to gel filtration (Superose 6 10/300 GL, GE Healthcare) in running buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The desalted protein containing fractions were pooled and loaded on a Q-Sepharose XK16/32 column (GE Healthcare) equilibrated with 25mM MES pH 5.5 as running buffer ...
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bioRxiv - Biochemistry 2019Quote: ... cleaved proteins were concentrated and finally purified by gel size exclusion chromatography (Superdex G75 26/60, GE Healthcare) in buffer C.
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bioRxiv - Immunology 2019Quote: ... The eluted protein was concentrated and loaded on a Superdex 200 16/60 column (GE Healthcare, Chicago, IL) pre-equilibrated with PBS ...
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bioRxiv - Cell Biology 2019Quote: GST tag was removed from GST-Ana2 by incubating the resin-bound protein with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amount of 5-10µg proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Membranes were revealed using a chemiluminescent HRP substrate (Millipore ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was further purified by size exclusion chromatography (SEC) (HiLoad 16/600 Superdex 200 pg, GE Healthcare) in 50 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... Protein containing fractions were loaded on SEC Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated with SEC buffer (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... and O-glycosylated proteins isolated by affinity chromatography using Concavaline A columns (HiTrap Con A 4B, GE Healthcare) in AKTA FPLC (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare; Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” (Perkin Elmer ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were visualized using the enhanced chemiluminescence (ECL) system (ECL™ Prime Western Blotting System, GE Healthcare Bioscience).
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bioRxiv - Microbiology 2019Quote: ... Anti-CFP polyclonal antibodies were purified by affinity chromatography using a HiTrap Protein A HP column (GE Healthcare) according to manufacturer instructions.
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bioRxiv - Microbiology 2019Quote: ... Membranes were solubilized in 1% (w/v) LMNG and proteins were purified using a StrepTrap column (GE Healthcare). The resin was washed in TBS containing 0.01% (w/v ...
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bioRxiv - Cell Biology 2019Quote: Protein purification was performed by immobilized metal-affinity chromatography (IMAC) on HisTrap HP Ni2+ Sepharose columns (GE Healthcare) at 4°C according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... fritted deep-well plates were used containing 25 μl of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Cell Biology 2020Quote: ... The processed proteins were concentrated and further purified by size exclusion chromatography (Superdex-75 16/60, GE Healthcare) in running buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... Supernatants were collected by centrifugation and purified using a 1 ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5 ml kappa-select column (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... The fractions containing protein were further using SEC column HiLoad 16/60 Superdex 75 prep grade (GE Healthcare) previously equilibrated with 50 mM KH2PO4 ...
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bioRxiv - Bioengineering 2021Quote: ... The protein was purified through nickel-agarose affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare). The purified protein was concentrated to 30 mg/mL in a buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Bioengineering 2021Quote: The purified protein was desalted in deionized Milli-Q water using a PD-10 column from GE healthcare and concentrated fivefold using a Thermo scientific three kDa MWCO protein concentrator ...
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bioRxiv - Biochemistry 2021Quote: ... the intact protein was further purified by cation exchange chromatography using a HiTrap SP HP column (GE Healthcare) and eluted by the 50– 500 mM NaCl gradient in 10 mM HEPES–KOH pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The α-domains of the proteins were purified to homogeneity by size exclusion chromatography (Superdex 75 GE Healthcare) equilibrated in 25 mM HEPES pH 7 ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel filtration with a Superose 6 10/300 GL column (GE Healthcare). hPCNA was expressed and purified as described in (Duffy et al ...
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bioRxiv - Biochemistry 2020Quote: All of the proteins characterized here eluted as homodimers as determined by a calibrated Superdex 75 (GE Healthcare) gel filtration chromatography column (25 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: Protein (450 μg/100 μL) was loaded on a Superdex™ 200 HR 10/30 column (GE Healthcare) equilibrated with PBS (140 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The tag-free protein was further purified through ion-exchange chromatography on a Heparin HP column (GE Healthcare) and size exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel-filtration chromatography on a Superdex 200 16/60 column (GE Healthcare) in Cas9 storage buffer (20 mM Tris-Cl ...
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bioRxiv - Cell Biology 2020Quote: ... the labeled protein was separated from the solution by size exclusion chromatography using PD MiniTrap G25 (GE Healthcare). PLCγ1 nSH2-cSH2-SH3 and truncations were labeled with CoA 647 (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was subsequently loaded into an ion exchange column (HiTrap® SP FF GE Healthcare Life Sciences), pre-equilibrated in the SP buffer A to remove 6His-ZB tag and uncleaved protein ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified from conditioned medium by cation exchange chromatography on a SP sepharose column (GE healthcare) in 50 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins separated by SDS-PAGE were detected following the manufacturer’s guidelines (GE Healthcare ECL Western Blotting detection system) and the images collected using a Chemidoc imaging system (BioRad Laboratories).
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further purified on a HiLoad 16/600 Superdex 75pg or 200pg gel-filtration columns (GE Healthcare). To obtain stoichiometric MLL1RWSAD complex ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 cells as described previously.13 The protein was purified using glutathione-Sepharose 4B beads (GE Healthcare) concentrated and flash-frozen in GST buffer [25 mM Tris (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... while the supernatant of GST-fusion protein homogenates were loaded onto pre-equilibrated glutathione SepharoseTM beads (GE healthcare), and incubated on an orbital rotator for an hour in cold room.