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Citations for GE Life Sciences :
3001 - 3050 of 4670 citations for Rat Androgen dependent TPF1 regulating protein ADTRP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... proteins were affinity purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast flow resin (GE Healthcare). Supernatant was two-fold diluted with 1xPBS (pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... for between 1 - 4 days and the protein signal visualized using a Typhoon Trio Variable Mode Imager (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were detected with horseradish peroxidase–conjugated anti-mouse or anti-rabbit antibodies (GE Healthcare Life Sciences, Marlborough, MA).
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bioRxiv - Microbiology 2022Quote: ... IgG1 and IgG2 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: All chromatography steps were performed at 4 °C using the ÄKTAxpress and ÄKTA pure protein purification systems (GE Healthcare). For affinity purification of the ADGRG4 construct ...
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bioRxiv - Bioengineering 2022Quote: ... All proteins were further purified by size-exclusion chromatography using an S75 or a S200 Increase column (GE Healthcare), depending on protein size ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by size exclusion column (SEC) using HiLoad 26/600 Superdex 200 (GE Healthcare, USA) in SEC buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Eluted protein was further purified by size exclusion chromatography (SEC) using Superose 6 Increase 10/300 column (GE Healthcare), pre-equilibrated with the desired buffers.
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bioRxiv - Biophysics 2022Quote: ... Protein was further purified by size exclusion chromatography using a Superose 6 Increase column (10/300 GL, GE Healthcare) equilibrated with 150 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
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bioRxiv - Plant Biology 2022Quote: ... Dialyzed protein was then loaded onto a 5 ml HiTrap Q HP anion exchange column (GE Healthcare Life Sciences) and eluted with a gradient of 0-100% elution buffer (50 mM Tris-HCl (pH 8) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were visualized with the LAS-4000 system (ImageQuantTM LAS-4000, GE Healthcare Life Sciences, Pittsburgh, PA, USA) after a chemiluminescent reaction (Immobilon® Western Chemiluminescent HRP Substrate ...
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bioRxiv - Immunology 2022Quote: ... the protein-containing supernatant was purified by nickel-affinity and size-exclusion chromatography on an AKTAxpress system (GE Healthcare), which consisted of a 1 mL nickel affinity column followed by a Superdex 200 16/60 gel filtration column ...
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bioRxiv - Immunology 2022Quote: ... the protein was further purified by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... The eluted protein was concentrated and purified by gel-filtration chromatography (Superdex 200 10/300 GL, GE Healthcare, USA), using a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... The cleaved protein was collected and further purified using gel filtration (HiLoad 16/60 Superdex pg 200, GE Healthcare) with GF buffer (20 mM Tris-HCl ...
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: ... and then purified through affinity chromatography using a 1 mL HiTrap Protein G HP column (GE Healthcare Life Sciences). Antibodies were eluted from the column using 0.1 M glycine-HCl ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: Filtered soluble recombinant wild-type RBPMS-A and mutants were purified on an AKTA protein purification system (GE Healthcare;). Recombinant protein was first purified on HisTrap HP column (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated on a 7.5% Bis-Tris gel and blotted onto a 0.45 μm nitrocellulose membrane (GE Healthcare Life Sciences ...
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bioRxiv - Physiology 2023Quote: ... Lipoproteins were fractionated using a Superose 6 10/300 GL fast protein liquid chromatography (FPLC) column (GE healthcare, UK). Fractions (500 μL ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The extracted mononucleosome fraction was incubated for 2 h at 4°C with Protein G Sepharose beads (GE Healthcare), which were preincubated with rabbit polyclonal anti–chicken CENP-A antibody64 ...
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bioRxiv - Molecular Biology 2024Quote: Protein lysates were separated on 10% SDS-PAGE gels and then transferred to PVDF membranes (GE Healthcare Life Science). The membranes were blocked in phosphate-buffered saline with Tween 20 (PBST ...
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bioRxiv - Biochemistry 2024Quote: ... and proteins in the soluble fraction were purified by Ni-affinity column chromatography (HisTrap 5 ml column, GE Healthcare). The His6-tag was removed by thrombin digestion (1 μg of thrombin per mg of TelSi3 protein ...
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bioRxiv - Biochemistry 2024Quote: ... uncleaved protein as well as cleaved GST were removed by applying everything onto 5 ml SP column (GE Healthcare) equilibrated with 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 300 µg of total protein extracts were purified using Streptavidin Sepharose High-Performance beads (GE Healthcare Bio-Sciences AB). For western blot analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare) for 2 hours at 4°C in a rotating wheel ...
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bioRxiv - Neuroscience 2024Quote: ... Protein extracts were separated in SDS-PAGE gels and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA). Western blotting was performed according to standard protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... was used at 1:10,000 and proteins were visualised by enhanced chemiluminescence using the ECL-Plus system (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: Proteins were further purified by size-exclusion chromatography (SEC) using a Superdex 200 10/300 GL column (GE Healthcare) integrated in the NGC system ...
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bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant containing the cleaved protein was removed and applied to a size exclusion (SEC) column (HiLoad_16/60_Superdex75 prep- grade, GE Healthcare) equilibrated with PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were then further purified by size-exclusion chromatography using a Superdex 75 300/10 column (GE Healthcare) with running buffer 20 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane (BTP) ...
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bioRxiv - Molecular Biology 2023Quote: The extract (supernatant after high-speed ultracentrifugation) was used for protein purification using the ÄKTA Xpress system (GE Healthcare), employing nickel affinity chromatography on the 5 mL column compatible with ÄKTA ...
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bioRxiv - Microbiology 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superdex 75 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 20 mM Hepes pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superdex 200 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 50 mM Hepes pH 7 ...
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bioRxiv - Immunology 2022Quote: ... the protein-containing supernatant was purified by nickel-affinity and size-exclusion chromatography on an AKTAxpress system (GE Healthcare), which consisted of a 1 mL nickel affinity column followed by a Superdex 200 16/60 gel filtration column ...
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bioRxiv - Cell Biology 2023Quote: ... soluble proteins were applied to the size exclusion gel filtration column (Superdex 200 Increase 10/300 GL, GE Healthcare), using an Akta P-920 Pump FPLC system (Amersham ...
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bioRxiv - Cancer Biology 2023Quote: ... and IgGs were then purified from the culture supernatants by Protein A-column chromatography (GE Healthcare, 17-1279-01). We obtained 6 clones as follows ...
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bioRxiv - Biophysics 2023Quote: ... The untagged protein was purified using gel filtration chromatography (ÄKTA with Superdex-200 increase 10/300 column; GE Healthcare) and equilibrated with storage buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 200 μL of protein solution were loaded onto a Superdex 200 HR 10/30 gel filtration column (GE Healthcare) with a flow rate of 0.5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence microscopy data of intracellular GFP fusion proteins were collected using a DeltaVision microscope system (GE Healthcare Inc., USA) equipped with a pco.edge 4.2 sCMOS camera (PCO ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated with SDS-PAGE and subsequently transferred to Amersham Hybond-P PVDF Transfer Membrane (RPN303F, GE Healthcare). Membranes were blocked in 5% milk in Tris-buffered saline with 0.01% Tween 20 and incubated with the antibodies listed in Supplemental Table 4 ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were removed by centrifugation and proteins were purified from the conditioned media using rProtein A Sepharose (GE healthcare). The purified proteins were buffer-exchanged into a formulation buffer (20 mM His-HAc ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining purification steps were carried out at 4 °C using an ÄKTA protein purification system (GE Healthcare/Cytiva) for column chromatography steps ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies (anti-rabbit IgG, anti-mouse IgG or protein-A) coupled with horseradish peroxidase were from GE Healthcare, an anti-goat IgG secondary antibody was from Santa Cruz.
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bioRxiv - Biophysics 2023Quote: ... Proteins were concentrated by centrifugation in Vivaspin 20 ultrafiltration devices with a 100,000 molecular weight cut-off (GE Healthcare). Protein concentrations were quantified by UV spectroscopy with an extinction coefficient of 36380 M-1cm-1 (Drp1 ...