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Citations for GE Life Sciences :
2901 - 2950 of 4670 citations for Rat Androgen dependent TPF1 regulating protein ADTRP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The protein was further purified by size-exclusion chromatography through a HiLoad 26/60 Superdex 200 column (GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... The 6xHis-tagged protein samples were loaded onto pre-equilibrated Ni2+ affinity resin (HisTrap HP 5 ml, GE Healthcare). The column was sequentially washed with HSU buffer containing 0.5% triton-X ...
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bioRxiv - Biophysics 2020Quote: ... SEC-MALS was performed at room temperature by passing 500 μl protein (12μM) at 0.3 ml/min flow rate through a Superdex S200 SEC column (GE Healthcare) pre-equilibrated with 10mM potassium phosphate buffer supplemented with 150mM NaCl and 2mM β-mercaptoethanol (pH 8.0) ...
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bioRxiv - Biophysics 2020Quote: ... Protein fractions (eluted at ~ 1 M NaCl) were then purified using a HiLoad 16/60 Superdex 75 (GE Healthcare) column (SEC ...
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bioRxiv - Cell Biology 2021Quote: Tag-free syndapin I and III were generated by expressing both proteins in the pGEX-6P vector (GE Healthcare) and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to nitrocellulose membranes (Amersham™ Protran™ Premium 0.45 μm NC, GE Healthcare Life sciences, 10600003). For Msb2p-HA and Hkr1p-HA blots ...
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bioRxiv - Cancer Biology 2020Quote: ... All protein lysates were run on NuPage 4–12% gradient gels (LifeTechnology) and transferred to PVDF membrane (GE Healthcare). Membranes were blocked in 5% milk in TBST and stained with primary antibody overnight at +4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by ion-exchange chromatography using the HiTrap Heparin HP column (GE Healthcare Life Sciences) and a Mono Q anion exchange column (16/10 ...
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bioRxiv - Biochemistry 2022Quote: ... The untagged protein was concentrated and further purified on a Superdex 200 16/600 gel filtration column (GE Healthcare) in 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The histidine-tagged cleaved proteins where then loaded onto a 26/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with buffer C ...
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bioRxiv - Biochemistry 2022Quote: ... Then the interest protein was purified by gel-filtration chromatography using a Superose 6 gel filtration column (GE Healthcare) pre-equilibrated with HBS buffer (10 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fusion constructs of each receptor were captured using either a Series S Protein A sensor chip (GE Healthcare) or a Series S CM5 sensor chip (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... fractions containing pure protein sub-complexes were subjected to size-exclusion chromatography (Superose 6 increase 10/300, GE Healthcare) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted proteins were cleaved overnight with 3C protease followed by gel filtration on Superdex 200 16/60 (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Proteins were then transferred onto a nitrocellulose membrane (Amersham™ Protran™ 0.2 µm NC, GE Healthcare Life science) using a standard blotting procedure (80V for 2h ...
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bioRxiv - Biochemistry 2022Quote: ... after which protein-containing fractions were pooled and loaded onto pre-equilibrated Superdex 200 Increase 10/300 (GE Healthcare) for size exclusion chromatography ...
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bioRxiv - Microbiology 2022Quote: ... Proteins released in the soluble form were purified using Glutathione Sepharose 4B according to the manufacturer’s instructions (GE Healthcare). On-column thrombin digestion was performed to release the protein without the GST tag ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... C (W6/32) and rotated with 30 μl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved by SDS-PAGE using 15% gels and transferred onto nitrocellulose membranes (Amersham Protran, GE Healthcare, USA). Immunoblots were processed by enhanced chemiluminescence (ECL ...
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bioRxiv - Biophysics 2022Quote: ... All the eluted proteins were loaded into Superdex 200 pg or Superdex 75 pg size exclusion chromatography (GE Healthcare) to remove aggregates and potential contaminants.
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bioRxiv - Immunology 2022Quote: ... 5% Glucose and the proteins purified by cation-exchange chromatography using a HiTrap SP High-Performance columns (GE Healthcare), followed by size exclusion chromatography using a HiLoad 16/600 Superdex 75pg column (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were further purified by size-exclusion chromatography using an S200 Increase or a Superose S6 column (GE Healthcare), depending on protein size ...
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bioRxiv - Biochemistry 2020Quote: ... protein containing eluate was concentrated and applied to a HiLoad™ 16/600 Superdex™ 75pg column (GE Healthcare) in 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The solution was added to 250 μL (bed volume) Protein G Sepharose™ 4 Fast Flow resin (GE Healthcare) prewashed with DPBS ...
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bioRxiv - Microbiology 2021Quote: ... and the proteins were visualized by enhanced chemiluminescence (ECL) using the ECL Prime Western blotting detection reagent (GE Healthcare) and the ChemiDoc™ Touch Imaging System (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... the protein of interest was purified by ion-exchange chromatography using MonoQ 4.6/100 PE column (GE Healthcare, Poland) with a linear gradient of 0-100% 50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing the protein was concentrated and applied to a SuperdexTM 200 10/30 GL column (GE Healthcare) and eluted with Buffer F (25 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20 mM citric acid pH 5.0 ...
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bioRxiv - Biophysics 2020Quote: ... Protein was purified by Ni2+ affinity chromatography followed by size-exclusion chromatography on a Superdex 75 column (GE Healthcare) equilibrated in 20 mM Hepes pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was collected and filtered using 0.22µm membrane filters and loaded onto HiTrap MabSelect SuRe Protein A (GE Healthcare) column pre-equilibrated against PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Extracts (15⍰μg protein) were separated by SDS–PAGE and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL, USA). Membranes were blocked in TBS (150⍰mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Neuroscience 2020Quote: ... GST-hMeCP2 or GST-OGT fusion proteins were immobilized on glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) and washed three times with GST binding buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant GST-tagged protein in the supernatant was purified using glutathione-Sepharose 4B beads (GE Healthcare, 17-0756-01), washed in 10 mM PBS (pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: Differential scanning calorimetry (DSC) was carried out on the S proteins using a MicroCal VP-DSC instrument (GE Healthcare, MicroCal Products Group ...
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bioRxiv - Immunology 2020Quote: ... The protein was then further purified by size exclusion chromatography using a Superdex 75 10/300 column (GE Healthcare). Ramos B cells expressing VRC01 germline B cell receptor were provided by Daniel Lingwood (Ragon Institute).
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bioRxiv - Microbiology 2021Quote: ... Twenty-five µL of Protein A Sepharose beads (50% slurry contained phosphate-buffered saline buffer) (GE healthcare, Chicago, IL) was added to the resultant supernatant ...
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bioRxiv - Biochemistry 2021Quote: Expressed CYP168A1 was purified using fast protein liquid chromatography with a HisTrap-HP affinity column (GE Healthcare, Chicago, IL). The bacterial cell pellets were thawed on ice and resuspended in buffer A consisting of 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... all proteins were further purified using size-exclusion chromatography on a Superdex-200 column (GE Healthcare, Buckinghamshire, United Kingdom). Their corresponding elution profiles are shown in Figure S12 ...
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bioRxiv - Bioengineering 2020Quote: The eluted proteins were concentrated and subjected to size-exclusion chromatography (Superose 6 Increase 10/300 GL, GE Healthcare) in the PBS buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were typically resolved on 7.5% TGX stain-free gels and transferred onto nitrocellulose membrane (GE Healthcare, Amersham Protran) using wet-transfer at 100 V for 60 min ...
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bioRxiv - Microbiology 2022Quote: ... and for HLA-I IP 25 μl Protein G Sepharose® 4 Fast Flow (GE Healthcare, GE17-0618-01) were employed with the HC10 antibody for HLA-IP overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Protein fractions were pooled for buffer exchange employing a PD-10 Sephadex™ G-25 desalting column (GE Healthcare) and centrifugal filters (Amicon®Ultracel®-10 K/30 K ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a 7.5% Bis-Tris gel and blotted onto a 0.45 µm nitrocellulose membrane (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... the protein was applied to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg, GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were first purified with Ni Agarose 6 FF (AOGMA) with the AKTA system (GE Healthcare Life Sciences) and further purified with anti-Flag affinity beads ...
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bioRxiv - Plant Biology 2019Quote: ... pastoris and the peptide was purified from the culture medium using the Fast Protein Liquid Chromatography System (GE Healthcare) and Reverse Phase-High Performance Liquid Chromatography (RP-HPLC ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant catalytic domain of PknB was purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) as previously described5.