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Citations for GE Life Sciences :
3001 - 3050 of 4130 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The cells were disrupted by sonication and the soluble part of the lysate was purified on a 5 mL-HiTrap heparin column (GE Healthcare) at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were seeded at ∼ 7.93 × 108 total viable cells (around 7.93 × 105 viable cells/mL) in a chemically defined media (ActiPro media; GE Healthcare, USA). The cultures were maintained with setpoints for pH at 7.2±0.05 ...
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bioRxiv - Immunology 2022Quote: ... Peak fractions were pooled and purified by anion-exchange on a 5 ml Hitrap Q (Cytiva, formerly GE Healthcare Life science) in a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Molecular Biology 2023Quote: ... Further protein purification was achieved by performing size-exclusion separation of 5 mL of the eluate from the affinity chromatography step on a Hiload 16/60 Superdex S200 column (GE Healthcare), with the use of 1.2 column volumes of gel-filtration (GF ...
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bioRxiv - Microbiology 2023Quote: ... The TEV digested proteins were separated from the cleaved His-tag product on an AKTA Pure 25L FPLC using a 5 mL HisTrap-FF with the captured flowthrough further purified using a HiLoad Superdex 75 PG gel filtration column (GE Healthcare). A monodisperse peak was obtained and assessed for purity using SDS-PAGE gel analysis ...
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bioRxiv - Microbiology 2023Quote: ... PmScsC-EcDsbA was initially purified by immobilized metal affinity chromatography (IMAC) using a 5 mL HisTrap Fast Flow column (GE Healthcare) followed by cleavage of the N-terminal His6-tag with TEV protease and reverse IMAC ...
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bioRxiv - Microbiology 2023Quote: ... 3 mg of proteins was used for immunoprecipitation with 50 ml of immunoglobulin G-Sepharose resin (IgG Sepharose 6 Fast Flow, GE Healthcare) or Anti-HA affinity matrix beads (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant was clarified using 0.45 µm filters before loading onto a nickel-Sepharose Fast Flow column (5 mL of bed volume) using an ÄKTATM Start Fast Protein Liquid Chromatography system (GE Healthcare). The column was washed with 40 mL of extraction buffer ...
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bioRxiv - Biochemistry 2022Quote: ... were solubilised in TK buffer supplemented with 6 M urea before loading onto a 5 ml HisTrap HP column (GE Healthcare). The column was first washed with TK + 6 M urea followed by TK + 6 M Urea + 50 mM imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... to a final concentration of 50 mM NaCl and loaded on an equilibrated 5 ml HiTrap Heparin HP Sepharose column (GE Healthcare). The protein was eluted using a linear 0–1 M NaCl gradient in buffer B ...
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bioRxiv - Biochemistry 2023Quote: ... Strep-tag purification (used for Rubisco-variants, see SI Table 5): Clarified lysate was applied to a 5 mL StrepTrap column (GE Healthcare) equilibrated with Strep-buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... injected (5 mL total) into HiLoad 16/600 Superdex 75pg (Cytiva) connected to an AKTA FPLC system (GE Healthcare Life Science). Gel filtration was performed using the same buffer to purify Nurr1-RXRα LBD heterodimer for analysis ...
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bioRxiv - Biochemistry 2023Quote: ... DAP5 was first purified from bacterial cell lysate using a 5 mL His-Trap HP (Ni-NTA) column from GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... rPF4 was purified from soluble fractions of cell lysates by affinity chromatography using a 5-ml HiTrap heparin-agarose column (GE Healthcare). Endotoxin-free rPF4 was prepared as previously described 3 ...
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions from ion-exchange chromatography were filtered through a 5 µm syringe filter and loaded onto a 22 mL Source 15ISO hydrophobic interaction column (GE Healthcare) equilibrated in buffer C (50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... The protein was purified by ion exchange chromatography using DEAE sepharose followed by size exclusion chromatography using a 60 ml Superdex 75 increase column (GE healthcare) in 6M GuHCl ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... The soluble fraction was filtered through a 0.45 μm sterile syringe filter and loaded onto a pre-equilibrated 5 mL HiTrap IMAC HP column (GE Healthcare Biosciences). The column was washed with 4 column volumes of lysis buffer followed by 4 column volumes of wash buffer 1 [50 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... The flow through was then concentrated in an Amicon Ultra-15 50,000 MWCO spin concentrator to ∼10-20 mg/mL and loaded onto a HiPrep 26/60 S200 Size Exclusion Column (GE Healthcare), equilibrated in Buffer D ...
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bioRxiv - Biophysics 2023Quote: ... The His6-SUMO-14-3-3ζ fusion protein was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow resin (5 mL; GE Healthcare) with standard IMAC procedures of sample application ...
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bioRxiv - Molecular Biology 2023Quote: ... further clarified using 0.2 µm filter and loaded on 5 ml Ni-NTA Sepharose high-affinity pre-packed column (His TrapTM, GE healthcare, Sweden), which was pre-equilibrated with binding buffer (20 mM Tris-Cl ...
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bioRxiv - Cell Biology 2023Quote: ... Rab35BD and Rac1BD-Rab35BD) (40 µL; 10-25 mg/ml) were loaded onto a Superdex-200 increase 5/150 column (GE Healthcare) or BioSec5 300 (Agilent ...
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bioRxiv - Biophysics 2023Quote: ... lysed with a high-pressure homogenizer and Fab was purified with a 5-ml HiTrap Protein G HP column (GE Healthcare). Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0 ...
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bioRxiv - Cell Biology 2023Quote: ... and pure fractions were pooled and concentrated before being concentrated and snap-frozen in liquid nitrogen GST-tagged RAB7A was similarly isolated via passing cleared lysate over a gravity column packed with 5 mL of Glutathione Sepharose 4B (GE Healthcare), followed by elution using 10 mM reduced glutathione dissolved in 50 mM Tris-HCl pH 8.0. ...
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bioRxiv - Neuroscience 2023Quote: ... and pellets were resuspended in 6 mL 25 % Standard Isotonic Percoll (Percoll: GE Healthcare, 17-5445-02 with 10% 10x PBS) in Medium A ...
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bioRxiv - Biochemistry 2022Quote: ... The protein-containing supernatant was collected and filtered through a 0.45 μm size cut-off filter and applied onto a 5 mL His Trap™ crude FF column (GE Healthcare), running in equilibration buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and the soluble His6-M protein was purified on a Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Biochemistry 2023Quote: The filtered cell-free extract was subjected to immobilized metal affinity chromatography using an Äkta pure chromatography system equipped with a 5-mL HisTrap HP column (GE HealthCare) equilibrated with lysis buffer (see above) ...
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bioRxiv - Biophysics 2023Quote: ... Q7 or Q20 at a concentration of ~4-7mg/ml were loaded onto an S200 Increase size-exclusion column (Superdex 200 Increase10/300 GL, GE Healthcare) at a flow rate of 0.5 ml min-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Capturing of ORF1p-His6 protein was performed using Immobilized-Metal Affinity Chromatography (IMAC) by loading of 7M Urea Extract on 5 mL HisTrap FF FPLC column (GE Healthcare) at 0.5 mL/minute flow rate followed by washing with 10 column volumes (CV ...
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bioRxiv - Biophysics 2023Quote: ... diluted with the same buffer devoid of NaCl to reach a final salt concentration of 40 mM and loaded on a HiSure Q 5 mL column (GE Healthcare). After washing with a 25 mM Na-HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were then incubated over night at 4 °C with appropriate antibodies (1ug/ml) and protein A Sepharose beads (GE Healthcare). After incubation samples were washed five times with lysis buffer and resuspended in 1X Laemmli sample buffer (Bio-Rad cat ...
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bioRxiv - Biochemistry 2023Quote: GB1-FKBP-ICD and GB1-FRB-ICD were mixed at equimolar ratio and loaded onto a 24-mL Superdex 200 column (GE healthcare) equilibrated in 100 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was cleared by centrifugation at 20000g for 45 min and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–300 mM in 30 column volumes ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then subjected to size exclusion chromatography in 20 mM tris pH 8.0 using a Superdex-75 HR 10/30 column fractionating at a flow rate of 0.5 mL/min and attached either to an AKTA FPLC system (GE Healthcare, Buckinghamshire) or a BioLogic DuoFlow system (BioRad) ...
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bioRxiv - Plant Biology 2024Quote: ... the untagged protein was separated from cleaved tags and the 6xHis-tagged 3C protease by affinity chromatography using 5 ml HisTrap HP NTA column (GE Healthcare). If proteins were also tagged with MBP ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) pre-equilibrated with the HiTrap binding buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was loaded onto a pre-washed/pre-equilibrated CM Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL) on an Äkta explorer FPLC (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The clear top phase obtained above a white band was collected and separated over a 5 ml HiTrap Q FF column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Cell Biology 2024Quote: ... The eluate was concentrated to a volume of 5 mL and loaded onto a size-exclusion-chromatography column (Superdex 16/60, GE Healthcare); HR3 LBD was eluted in a buffer of 150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) preequilibrated with the HiTrap binding buffer ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated using a 100 kDa filter to approximately 1 mL and further purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer containing 0.06% digitonin ...
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bioRxiv - Biochemistry 2024Quote: ... Concentrated eluates were then applied to a 24-mL Superdex S200 increase 10/300 GL size exclusion column (GE Life Sciences). Fractions containing Cdc13 (Fig ...
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bioRxiv - Biochemistry 2024Quote: ... at 8000 × g then subjected to two rounds of buffer exchange using equimolar Tris-KCl buffer and a 5-mL desalting column (HiTrap Desalting; GE Healthcare) pre-equilibrated with four column volumes of Tris-KCl ...
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bioRxiv - Biochemistry 2024Quote: ... The cleaved sample was passed through an equilibrated Nickel-charged IMAC using a 5 mL HisTrap HP (GE Healthcare Life Sciences), and the flowthrough was collected ...
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bioRxiv - Biochemistry 2024Quote: ... Final purification was performed with size exclusion chromatography using a Superdex 200 pg 16/600 gel filtration column (120 ml bed volume, GE Healthcare). At every purification step ...
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bioRxiv - Immunology 2024Quote: ... An affinity matrix was prepared by coupling 50 mg of His-tagged GST-RAP to 20 ml of NHS-activated Sepharose 4 Fast Flow (GE Healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Solid-phase syntheses of short oligonucleotides were performed in a 10 mL syringe equipped with frit and loaded with polystyrene support [ribo A 300 PrimerSupport 5G (299 μmol/g, GE Healthcare)] ...
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bioRxiv - Immunology 2020Quote: ... The supernatant was diluted 1:1 with 20 mM sodium phosphate pH 7.4 and then purified using HisTrap Excel columns (GE Life Sciences # 17-3712-05) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µL of a 1:1 mixture of hydrophilic and hydrophobic carboxylate coated paramagnetic beads (SeraMag Speed Beads, #44152105050250 and #24152105050250, GE Healthcare, Little Chalfont, UK) were added to 30 µg protein of each sample ...
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bioRxiv - Neuroscience 2020Quote: ... in which they were precleared for 1 h using 30 μl (dry volume) of a 1:1 mixture of protein-A and protein-G-agarose beads (GE Healthcare, Chicago, IL, USA). Thereafter ...