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Citations for GE Life Sciences :
2951 - 3000 of 4130 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The fractions corresponding to the second peak in the chromatogram (elution volume 70-75 ml) were pooled and subjected to further size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in the same buffer ...
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bioRxiv - Biochemistry 2020Quote: ... After concentration by spin filtration to 4-5 mL pro-rhodesain was loaded onto a HiLoad 16/600 Superdex 75 pg column (GE Healthcare), and purified by SEC (0.5 mL/min ...
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bioRxiv - Cell Biology 2021Quote: ... The purified recombinant proteins Spn2-BFP or BFP were concentrated to 2 ml and were further purified by SEC with a HiLoadTM 16/600 superdexTM 200 pg column (28-9893-35; GE Healthcare) in SEC buffer (50 mM Tris (pH 7.5) ...
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bioRxiv - Immunology 2020Quote: ... FP3 or FP4 from the Mono S column were pooled and further purified by SEC performed on a 24 mL Superdex 200 increase column (GE Healthcare) at 4 °C with a flow rate of 0.25 mL/min in a buffer containing 20 mM HEPES ...
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bioRxiv - Cell Biology 2019Quote: ... 100 µL of sample at 15 µM was injected from a static loop beginning at the 0 ml point of each run onto a Superdex 200 increase 10/300 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...
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bioRxiv - Biochemistry 2019Quote: ... A 50 – 500 mM KCl gradient was used to elute IF2 from a 5 ml Hi-Trap SP HP column (17115201, GE Healthcare). Fractions were analysed by 8% acrylamide SDS-PAGE and those containing IF2 were pooled together ...
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bioRxiv - Neuroscience 2019Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at - 80°C until use ...
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bioRxiv - Biochemistry 2019Quote: ... All Rab proteins were purified from the supernatant by nickel affinity chromatography (5 ml HiTrap™ Chelating HP column, GE Healthcare). Non-specifically bound proteins were removed by a step wash with buffer B (50 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... 50 mM NaCl to a final concentration of ∼3 mg/ml further purified by size-exclusion chromatography using a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer ...
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bioRxiv - Microbiology 2020Quote: ... Diafiltrated cell supernatant was purified by immobilized nickel affinity chromatography (5-ml fast flow crude column and ÄKTA fast protein liquid chromatography [FPLC] system; GE Healthcare) at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... coli and purified using our previously reported protocols.17 TNKS2 constructs were cloned into pNIC-MBP expression vectors and an additional purification on an MBPTrap HP 5 ml column (GE Healthcare) prior to cleavage with TEV protease was performed.20 Details of the constructs for each PARP enzyme are listed in Table S1.
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bioRxiv - Cell Biology 2022Quote: ... 5 min at 4 °C followed by an anion exchange chromatography loading the supernatant on a HiTrap Q HP 5 ml column (GE Healthcare) and intensive wash step of 20 column volumes with ion exchange buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT in the presence of TEV protease and subsequently purified using a 5 mL HiTrap HP Heparin column (GE Healthcare), eluting with a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: Purified SMO pCT was concentrated to 5 mg/ml and analyzed via SEC-MALS using a Superdex 75 gel chromatography column (GE Healthcare) equilibrated in gel filtration buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM NaCl) at approximately 3 mg/mL and then further purified by size-exclusion chromatography using a Superdex-200 column (GE Healthcare) equilibrated in solubilization buffer ...
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bioRxiv - Biophysics 2021Quote: ... AtGDH sample was concentrated and further purified with the Superdex 200 pg 16/600 gel filtration column (120 ml bed volume, GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2020Quote: ... Uncleaved protein and cleaved hexahistidine tag were removed from the eluted mixture with a HisTrap HP 5 mL column (GE Healthcare) in PBS with 25 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was concentrated and subjected to size exclusion chromatography on Superdex 200 increase semipreparative column (10/300, bead volume: 24 ml; GE Healthcare), also equilibrated with WB ...
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bioRxiv - Microbiology 2020Quote: ... The quality of the amphipol associated complex was controlled by performing an additional size exclusion step on a Superdex 200 increase column (10/300, bead volume: 24 ml; GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The cell debris was pelleted by centrifugation and the supernatant was loaded onto a 5 ml HisTrap IMAC HP Nickel Sepharose column (GE Healthcare), using an ÄKTA Pure chromatography system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were precipitated the supernatant by addition of ammonium sulfate to 67% saturation and dissolved in buffer D and loaded on equilibrated 5 mL HiTrap Heparin HP sepharose column (GE Healthcare). The protein was eluted with a linear 0-1 M NaCl gradient in buffer D ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was centrifuged at 13,000 g for 60 minutes and the filtered supernatant was loaded onto a 5 mL Ni-NTA column (GE Healthcare, USA) equilibrated with binding buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% glycerol) to the 50 mM NaCl final concentration and loaded on equilibrated 5 mL HiTrap Heparin HP sepharose column (GE Healthcare). The protein was eluted with a linear 0-1 M NaCl gradient in buffer B ...
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bioRxiv - Microbiology 2020Quote: ... Fractions corresponding to assembled nanoparticles (elution between 8.5-10.5 mL with a peak at 9 mL) were pooled and concentrated at 350 × g by spin filtration with Vivaspin filters (10 kDa molecular weight cutoff; GE Healthcare). 16055 SOSIP-I53-50NPs were then buffer exchanged into PBS supplemented with 250 mM sucrose using a Slide-A-Lyzer MINI dialysis device (20 kDa molecular weight cutoff ...
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bioRxiv - Microbiology 2022Quote: ... it was passed through a 0.22 µm filter to remove potential precipitates and applied onto a 5 ml HisTrap HP column (GE Healthcare, USA) equilibrated with buffer BZ3 as described above ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was centrifuged at 4 °C and 20000 g for 30 min and the supernatant applied to a gravity flow column with 4 mL pre-equilibrated Ni-NTA Sepharose resin (GE Healthcare). The column was washed with 50 mL of high-salt Tris-HCL buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... Undigested protein and TEV protease were removed by a second Ni-NTA step and flowthrough containing OCS was concentrated to 5 ml for subsequent gel filtration (Hiload 16/60 Superdex 200 pg, GE healthcare). Relevant fractions were pooled together ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein purification was performed on HisTrap FF crude 5 ml Ni-based column using ÄKTA Xpress System (Cytiva, former GE Healthcare). For that a wash buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Aggregates were removed by centrifugation (20 min, 48000xg, 4° C) and the protein solution loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 20 mM MOPS-NaOH pH 7.0 ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: Affinity-purified xtZnT8 was concentrated to 6-8 mg/ml and loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare), and was further purified by size-exclusion chromatography (SEC) ...
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bioRxiv - Biophysics 2022Quote: ... This was followed by size exclusion chromatography (SEC) using 24 ml Superdex 200 Increase 10/300 GL column on an AKTA-FPLC system (GE Healthcare). Analytical gel filtration was performed in 20 mM Tris pH 7.5 and 150 mM NaCl at the flow rate of 0.5 ml/min.
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was first purified by Immobilized Metal Affinity Chromatography (IMAC) by loading the clarified lysate onto a 5 mL Nickel column (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates from 8 L of yeast treated cells were then subjected to a 5 ml Nickel affinity purification using an AKTA purifier system (GE Healthcare) equilibrated in the urea buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The mTORC1 complex and free RAPTOR were separated by anion exchange chromatography on a 5 mL HiTrap Q column (GE Healthcare). The fractions containing mTORC1 complex and free Raptor were concentrated to 1.3 mg/mL and 0.5 mg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed and cleared lysate prepared as described and loaded onto a 5 mL Strep-Trap HP column (GE Healthcare), equilibrated with buffer D ...
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bioRxiv - Biochemistry 2022Quote: ... soluble His-GST-APLFAD (or His-SUMO-APLFAD) was loaded on a 5 mL HisTrap FF column (GE Healthcare Life Sciences), pre-equilibrated in lysis buffer (50 mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 10 mL 1X S basal buffer for COPAS biosorter analysis and imaging on a GE InCell analyzer 2200 (GE Healthcare). See Supplementary Methods for full details.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The urea concentration was set to 6 M and the sample was applied to a 5 mL HiTrap QFF anion exchange column (GE healthcare) and was eluted using a 30 CV long gradient from low salt buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2024Quote: ... the untagged protein was separated from cleaved tags and the 6xHis-tagged 3C protease by affinity chromatography using 5 ml HisTrap HP NTA column (GE Healthcare). If proteins were also tagged with MBP ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were centrifuged to remove cell debris at 20,000 x g for 60 min prior to loading of a 5 mL HiTrap SP-FF column on an ÄKTA purifier FPLC (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Molecular Biology 2024Quote: ... the unbound fractions were collected and applied to a Sours 15 Q ion exchange sorbent (column V = 8 mL, GE Healthcare) at a rate of 5 mL/min ...
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bioRxiv - Microbiology 2024Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) preequilibrated with the HiTrap binding buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) pre-equilibrated with the HiTrap binding buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was loaded onto a pre-washed/pre-equilibrated CM Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL) on an Äkta explorer FPLC (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: Size-exclusion chromatography coupled with multiple angle light scattering (SEC-MALS) experiments were performed using an S75 column (Superdex 75 10/300 GL 24 ml, GE Healthcare), Agilent HPLC system (Wyatt Dawn HELIOs II ...
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bioRxiv - Biochemistry 2024Quote: The quaternary structures were determined by size exclusion chromatography using a 25 mL Superdex 200 10/300 GL Increase column (GE Healthcare) for FDP_E ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was then filtered through 0.2 µm PES filter membrane and loaded onto a HisTrap HP column (5 ml, 1.6 x 2.5 cm, Ni Sepharose High-performance column, GE Healthcare, now Cytiva) pre-equilibrated with buffer A at 1 mL/min ...
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bioRxiv - Biophysics 2024Quote: ... were used with flow rates at 6 ml/min and loaded either by hand or by using an AKTA Purifier (GE Healthcare) following manufacturers guidelines ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... Proteins were further purified by concentrating to ∼3mg/ml and applying to a size exclusion column (Superdex-200 increase, 10/30 GE Healthcare) equilibrated in buffer A containing 5 mM ATP ...