Labshake search
Citations for GE Life Sciences :
2951 - 3000 of 5568 citations for Human IgM Anti Flavivirus Envelope Protein Antibody 4G2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... Protein purification was performed using glutathione sepharose 4B resin according to the manufacturer’s instructions (GE Healthcare). Eluted proteins were then dialyzed and concentrated using an Amicon Ultra-15 10 KDa cutoff (Millipore).
-
bioRxiv - Immunology 2023Quote: ... Recombinant IgG was harvested and purified after 6 days with protein G sepharose beads (GE Healthcare), dialyzed in PBS ...
-
bioRxiv - Immunology 2023Quote: ... Eluted protein was concentrated with 3 kDa MWCO filters (Amicon) before size-exclusion chromatography (GE Healthcare). Proteins were stable at 4ºC.
-
bioRxiv - Biochemistry 2023Quote: ... The protein-containing fractions were pooled and further purified on a Superdex 200 column (GE Healthcare), equilibrated ...
-
bioRxiv - Developmental Biology 2023Quote: ... The proteins were then transferred from the gel to the PVDF membrane (GE Healthcare Life-Science) in Bio-Rad vertical gel Transfer Apparatus using Transfer buffer (2.5mM Tris base ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Recombinant proteins were then purified by 1 mL Ni Sepharose (17-5318-01, GE Healthcare, USA). Columns were sequentially washed with 20 mL buffer 1 and 10 mL buffer 2 (in mM ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Biotinylated proteins were purified using SEC on a Superdex 200 10/300 Increase GL (GE Healthcare) or S75 10/300 Increase GL (GE Healthcare ...
-
bioRxiv - Biochemistry 2023Quote: ... The proteins were further purified by gel filtration on Superdex 75 pg 16/600 (GE Healthcare) in 25 mM Tris-HCl ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein detection was finally performed using Amersham ECL Western Blotting detection reagent (GE Healthcare #CA95038-564L).
-
bioRxiv - Immunology 2023Quote: ... The proteins were visualized by ECL-Plus (GEHealthcare) using Image Quant LAS500 (GE Healthcare Life Sciences). The following antibodies were used ...
-
bioRxiv - Plant Biology 2023Quote: Protein samples were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). After being blocked in 1 × PBST buffer containing 5% skimmed milk ...
-
bioRxiv - Plant Biology 2023Quote: ... The protein concentration was determined with 2-D Quant Kit (GE Healthcare Life Sciences, Westborough, USA) using Bovine Serum Albumin (BSA ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biophysics 2023Quote: ... Soluble protein was maintained at 4°C and purified using Ni-NTA affinity resin (GE Healthcare). A column wash was performed using pH 7.0 HisA containing 300 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... 25µL of Protein L beads (Cat. no. Capto™ L resin, GE Healthcare Cat. no. 17547802), pre-equilibrated with binding buffer (20mM HEPES ...
-
bioRxiv - Immunology 2023Quote: ... the protein was further purified by size-exclusion chromatography on a Sephacryl 300 column (GE Healthcare) in PBS.
-
bioRxiv - Bioengineering 2023Quote: ... Affinity purification of the protein was achieved using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer consisting of 100 mM tris-HCl ...
-
bioRxiv - Molecular Biology 2023Quote: ... A small portion of the final proteins were run through the Superdex 200 columns (GE Healthcare) again for quality check.
-
bioRxiv - Genomics 2023Quote: ... 167 mM NaCl) and pre-cleared with protein-A-Sepharose beads (GE Healthcare 17-5280-02) for 30 min at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... Samples were pre-cleared with 40 µl of protein G Sepharose beads (50% slurry, GE Healthcare) plus 5 μg Goat IgG (Invitrogen) ...
-
bioRxiv - Biophysics 2023Quote: ... The protein was injected onto a Superdex-75 Increase 10/300 GL column (GE Healthcare 29148721) for size exclusion chromatography in SEC buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Free copper was then removed by passing the protein through a HiTrap Desalting column (GE Healthcare). Prior to the SANS experiments ...
-
bioRxiv - Biophysics 2023Quote: ... The protein was further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2023Quote: ... The protein was loaded onto a HiLoad™ 16/600 Superdex™ 200 column (GE Healthcare) equilibrated with a buffer containing 30 mM Tris-HCl pH7.5 ...
-
bioRxiv - Biophysics 2023Quote: ... All proteins were further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Immunology 2024Quote: ... then further purified using Gammabind Plus Sepharose Protein G 25 mL column (GE Healthcare, Life Sciences), with 20 min contact time ...
-
bioRxiv - Biochemistry 2024Quote: ... Separation of the labeled protein from residual dye was carried out using a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer ...
-
bioRxiv - Bioengineering 2024Quote: ... Scaffolds and RSVF trimer or IgG proteins were immobilized on a CM5 chip (GE Healthcare # 29104988) by amine coupling ...
-
bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred overnight onto nitrocellulose membranes (Whatman, GE Healthcare Life Sciences, Buckinghamshire, UK) in a 1 litre tank containing transfer buffer at 30 V.
-
bioRxiv - Cancer Biology 2024Quote: ... mixed with 25 μl of protein A-Sepharose beads (GE healthcare, catalog no. 17-0469-01). The immunoprecipitated proteins were eluted from protein A-Sepharose beads by boiling with 2x sample buffer and subjected to SDS-PAGE.
-
Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing the target protein were pooled and loaded onto a Superdex S75 column (GE Healthcare) equilibrated in buffer containing 150 mM KCl ...
-
bioRxiv - Biochemistry 2024Quote: ... Protein for crystallization was further purified by size-exclusion chromatography on a Superdex 75 (GE Healthcare) with gel filtration buffer (50 mM Tris-HCl ...
-
bioRxiv - Microbiology 2024Quote: ... The protein extract was precleared by incubation with nProtein A-Sepharose 4 Fast Flow (GE Healthcare) for 30 min and then incubated with 20 uL of rabbit anti-Myc antibody (ab9106 ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were detected with enhanced chemiluminescence (ECL) detection reagents (RPN2236 or RPN2235, Amersham Biosciences, GE Healthcare,) and ChemiDoc™ XRS+ System (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2024Quote: ... proteins were separated by SDS-PAGE and transferred onto 0.45μm pore Protran nitrocellulose membrane (GE Healthcare) using semi-dry transfer apparatus (Hoeffer) ...
-
bioRxiv - Biochemistry 2024Quote: ... Then tag-cleaved proteins was purified with ion exchange chromatography (HiTrap SP FF column (GE Healthcare). Target proteins in the HiTrap SP FF column were eluted by a linear gradient of NaCl and further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare) ...
-
bioRxiv - Physiology 2024Quote: ... Target proteins were visualized by enhanced chemiluminescence using a commercially available kit (GE Healthcare, Freiburg, Germany).
-
bioRxiv - Microbiology 2024Quote: ... The proteins separated on the gels were transferred to nitrocellulose membranes (Amershan Protan membranes, GE Healthcare) using transfer buffer (0.192 M Glycine ...
-
bioRxiv - Developmental Biology 2021Quote: ... Membranes were subsequently incubated with the indicated primary antibodies followed by the appropriate horseradish peroxidase-labelled antibodies (1/10000, Sigma-Aldrich or GE Healthcare, see Supplementary table). Immunodetection was performed using Super Signal West Pico or Femto Chemiluminescence Kit (Pierce) ...
-
bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated goat anti-rabbit or anti-mouse IgG (NA9340-1ml or NA9310-1ml, GE Healthcare) was used as a secondary antibody ...
-
bioRxiv - Cell Biology 2021Quote: ... Anti-rabbit IgG-HRP (NA934) and anti-mouse IgG-HRP (NA931) were purchased from GE Healthcare Life Sciences ...
-
bioRxiv - Biochemistry 2023Quote: ... anti-rabbit IgG-HRP (NA934V) and anti-mouse IgG-HRP (NA931V) (GE Healthcare, GENA934 and GENA931).
-
bioRxiv - Cell Biology 2023Quote: ... Blots were reacted with anti-rabbit (or anti-mouse) immunoglobulin G conjugated to HRP (GE Healthcare) at 1:10,000 dilution ...
-
bioRxiv - Biochemistry 2019Quote: The interaction of the recombinant heterodimeric (‘monomer’) and homodimeric (‘dimer’) human G6b-B extracellular domain with different ligands was quantified using a BIAcoreTM 8K instrument (GE Healthcare, Little Chalfont, UK). Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare ...
-
bioRxiv - Immunology 2022Quote: ... or monocytes were isolated from buffy coat of healthy human donors (Biological Specialty Corp) by density centrifugation in Ficoll®-Paque PLUS (GE Healthcare Life Sciences). Monocytes were enriched either through positive selection using CD14 MicroBeads (Miltenyi Biotech ...
-
bioRxiv - Cell Biology 2020Quote: ... or donkey anti-rabbit (GE Healthcare NXA931 or Jackson Immunoresearch 711-035-152 ...
-
bioRxiv - Molecular Biology 2020Quote: ... anti-rabbit IgG (NA934, GE Healthcare), anti-rat IgG (31470 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Donkey anti-rabbit (GE Healthcare Biosciences) conjugated with horseradish peroxidase was used as a secondary antibody ...
-
bioRxiv - Cell Biology 2022Quote: ... or anti-mouse (NA931V, GE Healthcare). These were incubated for 1 hr at room temperature and blots were developed using Enhanced Chemiluminescence (ECL prime ...