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Citations for GE Life Sciences :
2851 - 2900 of 5568 citations for Human IgM Anti Flavivirus Envelope Protein Antibody 4G2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The recombinant proteins were purified from the filtrated supernatants using a HisTrap excel column (GE Healthcare). The purified proteins were biotinylated in vitro using the BirA enzyme in the presence of 0.5 mM Biotin and 10 mM ATP ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was purified using a 5 mL HisTrap HP column (GE Life Sciences, now Cytiva), using wash [0.05 M Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were blotted on a 0.45 μM pore size nitrocellulose membrane (GE Healthcare, Chicago, IL, USA) using a Transblot SD semi-dry transfer system (Bio-rad ...
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bioRxiv - Immunology 2020Quote: ... mAbs were purified directly from culture supernatants using HiTrap protein G columns (GE Healthcare Life Sciences) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... MuSK1B was purified from culture supernatants using HiTrap Protein G High Performance column (GE Life Sciences) equilibrated in 20 mM Tris pH 8.0 ...
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bioRxiv - Systems Biology 2019Quote: ... The cleaved proteins were further purified on a 5 mL HiTrap Q HP column (GE Healthcare) and then a Superdex 200 10/300 GL size-exclusion column ...
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bioRxiv - Biophysics 2019Quote: ... the protein was subjected to separation on the Ni sepharose 6 Fast Flow resin (GE Healthcare) using the same buffers as above to separate the protein from the cleaved tag ...
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bioRxiv - Cell Biology 2021Quote: ... 20 µg of GST fusion protein and 20 µl of Glutathione Sepharose 4B Beads (GE Healthcare) were incubated with 1 ml of HeLa cell extract for 2 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the protein was cleaned up using a Superdex 75 Increase 10/300 GL column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 8-15% SDS gels and transferred to a nitrocellulose membrane (GE Healthcare) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged expressed proteins were purified using GST beads (Glutathione Sepharose 4B; GE Healthcare Life Science). On the following day ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine) ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT) and bound protein was eluted directly onto a HiTrap Heparin column (GE Healthcare) using buffer Hep A supplemented with 2.5 mM Desthiobiotin ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were detected using Amersham enhanced chemical luminescence reagent as per the manufacturer’s instructions (GE Healthcare) and visualized by exposure to x-ray film.
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bioRxiv - Cell Biology 2021Quote: ... Full-length IgG was obtained by affinity purification utilizing a Protein A chromatography column (GE Healthcare) in AKTA avant (Cytiva) ...
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bioRxiv - Neuroscience 2021Quote: ... Biotinylated membrane proteins were purified using Streptavidin High Performance Spintrap columns (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Synthetic Biology 2021Quote: ... proteins were further purified by gel-filtration on a 10/300 Superdex S75 column (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... Protein purification was performed via affinity chromatography using Ni2+ charged Fast Flow Chelating Sepharose (GE Healthcare) as stationary phase ...
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Structure of mycobacterial cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidatesbioRxiv - Microbiology 2021Quote: ... Protein was then concentrated and loaded onto a Superdex 6 increase (10/300 GL, GE Healthcare) column equilibrated in buffer B ...
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bioRxiv - Neuroscience 2020Quote: ... The eluted protein was subjected to MonoQ 10/100 GL (GE Healthcare Lifesciences, Marlborough, MA, USA) chromatography using a 0-600 mM NaCl gradient elution ...
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bioRxiv - Biophysics 2020Quote: ... Endo180-Fc was purified to homogeneity by protein A affinity chromatography using MabSelect PrismA (GE Healthcare) followed by Capto QXP resins (GE BioSceinces ...
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bioRxiv - Biochemistry 2021Quote: ... The protein mixture was then purified through two tandem 5-ml MBPTrap HP columns (GE Healthcare). After loading ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins in the gel were transferred to polyvinylidene fluoride (PVDF) membrane (GE Healthcare Life Sciences). The membranes were blocked with 2% milk (Marvel ...
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bioRxiv - Cell Biology 2019Quote: Proteins were purified according to their His6-tag using a His Excel column from GE Healthcare attached to a Äkta Start system ...
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bioRxiv - Biochemistry 2021Quote: ... The size exclusion chromatography was run with the ÄKTA protein purification system (GE Healthcare Life Sciences), equipped with Superdex 75 10/300 GL and run with buffer solution (150 mM sodium chloride ...
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bioRxiv - Immunology 2020Quote: ... The concentrated protein elutes were run on a Superdex 200 Increase 10/300 GL (GE Healthcare) column on an Akta™Pure (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... dialyzed protein was transferred into Lysis Buffer with a HiPrep 26/10 desalting column (GE Healthcare) and passed over a HisTrap FF column ...
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bioRxiv - Immunology 2021Quote: ... mAbs were purified directly from culture supernatants using HiTrap protein G columns (GE Healthcare Life Sciences) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... The proteins were purified from the cell lysate using 300 μl GST resin (GS4B, GE Healthcare), and incubated with excess purified wild-type or mutant rDPP9 proteins on ice for 60 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μl of bait protein was mixed with 40 μl bed volume StrepTactin resin (GE Healthcare) and incubated for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The untagged protein was applied to the Superdex 200 increase 10/300 GL column (GE Healthcare) in SEC-buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted protein was concentrated and subjected to a HiLoad 16/600 Superdex 75 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was purified using Protein G Sepharose 4 Fast flow (GE Healthcare Life Sciences, Pittsburgh, PA) as per manufacturer protocol.
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bioRxiv - Immunology 2020Quote: ... Endo180-Fc was purified to homogeneity by protein A affinity chromatography using MabSelect PrismA (GE Healthcare) followed by Capto QXP resins (GE BioSceinces ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated and subjected to a HiLoad 16/600 Superdex 75 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... the protein without His6-tag was injected into a Superdex 75 10/300 GL (GE Healthcare) size exclusion column with the buffer B (50 mM NaH2PO4/Na2HPO4 pH 6.5 ...
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bioRxiv - Microbiology 2022Quote: ... Filtered lysates with strep-tagged proteins were applied onto StrepTrap HP 1 ml columns (GE Healthcare). Subsequent protein separation was conducted using the ÄKTA start chromatography system (GE ...
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bioRxiv - Neuroscience 2022Quote: ... The eluted protein was concentrated and further injected into a Superose 6 Increase column (GE Healthcare) for size-exclusion chromatography (SEC ...
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bioRxiv - Developmental Biology 2022Quote: ... The proteins were then transferred onto a Protran Nitrocellulose Membranes (0.45 µm pore size, GE Healthcare) via Power Blotter-Semi-dry Transfer System (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Abcam prestained mid-range protein ladder (Ab116027) and 0.45 µM Amersham Hybond PVDF membrane (GE Healthcare) were used ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein-containing fractions were pooled and further purified on a Superdex 200 column (GE Healthcare), equilibrated and eluted in tubulin buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein purification was performed on HisTrap HP 5 ml Ni-based column (Cytiva, former GE Healthcare) using ÄKTA Explorer System (Cytiva ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred (100 V, 1 hr) onto PVDF (polyvinylidene difluoride) membranes (GE Healthcare Life Sciences) using 1X Tris/Glycine buffer (in-house 50X solution containing 250 mM Tris and 1920 mM Glycine) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was resuspended in lyses buffer and inject into a Superdex 200 26/100 (GE Healthcare) pre-equilibrated with gel filtration buffer (20 mM Tris pH 7.8 ...
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bioRxiv - Biochemistry 2022Quote: ... cleaved protein was further purified by gel filtration using a Sephacryl S-400 column (GE Healthcare) with one of two buffers ...
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bioRxiv - Cell Biology 2022Quote: ... and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before88 ...
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bioRxiv - Physiology 2022Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred onto nitrocellulose membranes (GE Healthcare). The nitrocellulose membranes were blocked with 5% BSA in TBST (TBS containing 0.1% Tween20 ...
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bioRxiv - Microbiology 2022Quote: ... M28 IgG was then purified from the supernatant using Protein A affinity chromatography (GE Healthcare #17549851). IgG was then dialyzed overnight in TBS (150mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... Quantifications of protein levels were performed with an ImageQuant™ LAS 4000mini Biomolecular Imager (GE Healthcare), and images were analyzed with ImageJ software (NIH) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein constructs were concentrated with Vivaspin 20 5000molecular weight cut-off centrifuge tubes (GE Healthcare) and stored at -80 °C ...