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Citations for GE Life Sciences :
251 - 300 of 4168 citations for 7 Oxa 1 thia 3 azaspiro 4.4 non 2 ene 4 6 dione 2 amino 8 8 dimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... the membranes were incubated for 2 h in Can Get Signal Solution 2 (Toyobo) with anti-rabbit or mouse IgG conjugated to horseradish peroxidase (GE Healthcare). After washing twice with TBST ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 S-2P and SARS-CoV-2 HexaPro S were purified over a Superose6 Increase column (GE Life Sciences).
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bioRxiv - Molecular Biology 2021Quote: ... or 8 % denaturing polyacrylamide gel (PAGE) and then transferred to a nylon membrane (Biodyne® A, PALL or Hybond-N+, GE Healthcare). After transfer ...
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bioRxiv - Immunology 2021Quote: ... Reduced proteins were run on 8%,10% or 12% SDS-PAGE gels and transferred on to 0.22 μm nitrocellulose membranes (GE Healthcare Life Sciences). Membranes were blocked using 5% skim milk in 1X Tris-buffered saline and 0.1% Tween 20 (1XTBST ...
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bioRxiv - Biochemistry 2019Quote: ... 0.0027M KCl pH 8) and they were bound to a GST affinity column (GSTrap™ Fast Flow, GE Healthcare: 17-5131-02). The column was washed (0.02M NaPO4 ...
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bioRxiv - Cancer Biology 2020Quote: Protein expression on immunoblots was quantified on 8-bit gray-scale-transformed .tiff images of either scanned Amersham Hyperfilm MP (GE Healthcare, 28906838) or .tiff images obtained by the Bio-Rad ChemiDoc imaging system ...
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bioRxiv - Molecular Biology 2019Quote: ... Equal amounts of protein (10-50 µg) from the total extract were separated on 8-10% SDS-polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Germany) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein samples were separated on 8% Tris/glycine gels and then transferred to nitrocellulose membranes (GE Healthcare, Uppsala, Sweden). Subsequently the membranes were incubated with primary antibodies and HRP-coupled secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Synthetic Biology 2023Quote: ... and then inserted immediately upstream of a promoter-less chloramphenicol resistance gene (cat) gene in pBR322 origin a medium-copy-number plasmid pKK232-8 (GE Healthcare, USA) to create the pHL1141 plasmid (8 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples at 8-13 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples at concentrations >8 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 μL (24-well plates) or 0.6 μl (96-well plates) Dharmafect-1 (GE Life Sciences) in serum-free Minimum Essential Medium Eagle (MEM ...
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bioRxiv - Cell Biology 2020Quote: ... the corresponding cDNAs of mouse angulin-1 were amplified by PCR using KOD-Plus-Ver.2 DNA polymerase and subcloned into pGEX-6P-1 (GE Healthcare). To construct an expression vector for MBP-tagged N-ZO-1 (aa 1-862) ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted in 1 × TBST with 5% non-fat milk: anti-rabbit IgG (1:10,000; NA934V; GE Healthcare) and anti-mouse IgG (1:10,000 ...
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bioRxiv - Biochemistry 2023Quote: ... blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
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bioRxiv - Evolutionary Biology 2019Quote: ... on each FTA punch or on 2 µL or 4 µL of DNA solution using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life TM Sciences). Amplified DNA was purified with the SigmaSpin Sequencing reaction Clean-up (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2021Quote: ... and imaged with an IN Cell Analyzer 6000 (nine fields of view per well, 20x/0.45NA air objective, 2൸2 binning) (GE Healthcare Life Sciences). Following image acquisition ...
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bioRxiv - Immunology 2020Quote: SARS-CoV and SARS-CoV-2 ectodomain and RBD constructs were transiently transfected in Expi293F cells at a density of 2 million cells/mL using polyethylenimine and expression plasmids at a molar ratio of 3:1 and purified from clarified transfected culture supernatants 4-days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Proteins were eluted from the column using 250 mmol/L imidazole ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rabbit-IgG-F(ab’)2 (GE Healthcare) antibody for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rabbit-IgG-F(ab’)2 (GE Healthcare) antibody for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 2 ml of Chelating Sepharose Fast Flow (GE Healthcare) was added to a small plastic column and prepared for affinity purification by sequential washing with 1 column volume (CV ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2 mM DTT using PD10 columns (GE Life Sciences) and concentrated to 9 mg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM DTT by HiTrap desalting column (GE Healthcare).
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bioRxiv - Immunology 2019Quote: ... 4°C (SORVALL RC 6+) and supernatant was applied to a HisTrap affinity column (GE Healthcare) that was pre-equilibrated with binding buffer ...
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bioRxiv - Neuroscience 2020Quote: Derivatized-protein samples were analysed by two-dimensional (2D) gel electrophoresis using Immobiline® DryStrips pH 3-11 (IPG) non-linear (NL) 24 cm (GE Healthcare) for isoelectric focusing (IEF ...
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bioRxiv - Developmental Biology 2019Quote: ... 30 μg of proteins from lysates were subjected to migration on 8–12 % acrylamide gels and transferred on to polyvinylidene difluoride membranes (GE Healthcare Europe GmbH) in borate buffer (50 mM Tris-HCl and 50 mM borate ...
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bioRxiv - Neuroscience 2019Quote: Whole-brain T1-weighted MRI data were acquired using a General Electric HDx 3T MRI scanner and a 8-channel receiver head coil (GE Healthcare, Waukesha, WI) at the Cardiff University Brain Research Imaging Centre with an axial 3D fast spoiled gradient recalled sequence (echo time 3 ms ...
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bioRxiv - Biochemistry 2021Quote: ... Equal amounts of protein (10–50 μg) from the total extract were separated on 8–10% SDS–polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Chicago, IL) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... liver and enzyme treated lung were prepared by meshing organs through 70 μm nylon cell strainers in sRPMI-1640 with 8% FBS (HyClone Laboratories, GE Healthcare, Logan, Utah). After red blood cells lysis with NH4Cl (liver ...
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bioRxiv - Plant Biology 2022Quote: ... Twenty micrograms of total RNAs were separated on 15% acrylamide/8 M urea gel and transferred onto an Amersham™ Hybond™-N+ nylon membrane (GE Healthcare). The specific isotope probe was generated by end-labeling DNA oligonucleotides complementary to the miR167a sequence with [γ-32P] ATP by T4 polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: ... High resolution images of individual PvLS parasites and PHH nuclei were obtained by capturing 8 planes in the Z dimension using a 100×objective on Deltavision Core (GE Healthcare Life Sciences) and deconvoluted using softWoRx (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham, GE Healthcare Life Sciences). The membrane was blocked in 5% skimmed milk (HIMEDIA ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in non-fat 4% milk in TBS/T and incubated with primary and HRP-conjugated secondary (GE Healthcare) antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated at 4°C overnight with primary antibody (1:1000) and incubated for 1 hour at RT with secondary antibodies in either 2% PBA (GE healthcare, RPN418), 5% ELK or OBB (LI-COR ...
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bioRxiv - Biochemistry 2020Quote: ... 5’-FAM-UGUUCUUCUGGAAGUCCAGTT-3’) was separated by gel electrophoresis (20% polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT at a flow rate of 2 ml/min using an Akta purifier system (GE Healthcare), eluting at approximately 100 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... the sample was loaded at 2 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The NEC220 and NEC-CBM constructs were passed over 2 x 1 mL HiTrap Talon columns (GE Healthcare), rather than ion exchange as previously described ...
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bioRxiv - Synthetic Biology 2022Quote: ... for 1-2 minutes in darkness then exposed and imaged using an Amersham™ Imager 600 (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... for 1-7 days before acquiring radiograms using Typhoon 5 Imager (GE Healthcare). Densitometry was carried out using ImageJ53 ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and the NiRAN construct (nsp12 amino acid residues 1-259) were cloned into pGEX-6P-1 vector (GE Healthcare) with an N-terminal GST tag followed by a PreScission protease site ...
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bioRxiv - Developmental Biology 2021Quote: ... OTP non-targeting pool (GE Healthcare) was used as control siRNAs in all experiments ...
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bioRxiv - Cell Biology 2021Quote: ... 15-25 μg of total protein per sample were resolved on 8%-14% SDS-PAGE and transferred onto nitrocellulose membrane (Amersham Hybond, GE Healthcare Life Science, 10600002). Membranes were blocked in 5% milk in PBS followed by incubation with specific primary and secondary antibodies ...
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bioRxiv - Evolutionary Biology 2019Quote: ... ligation mix was diluted to 50µl and used 0.583:1 volume of home-brewed SPRI beads (SPRI binding buffer: 2.5M NaCL, 20mM PEG 8000, 10mM Tric-HCL, 1mM EDTA, oh=8, 1mL TE-washed SpeedMag beads GE Healthcare, 65152105050250 per 100mL buffer) for purification ...
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bioRxiv - Microbiology 2022Quote: ... were collected by vacuum filtration onto glass microfiber filters (grade GF/C, 47 mm in diameter, pre-combusted at 460°C for >8 h, GE Healthcare Whatman, Buckinghamshire, UK), immediately plunged into liquid nitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant desmin was purified under denaturing conditions (8 M urea) by ion exchange chromatography (IEC) using 5 mL HiTrap DEAE-FF columns (GE Healthcare, Chicago, IL, USA) and in a second step by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Biophysics 2019Quote: ... A drop of 5 μL of 2% dimethyldichlorosilane (GE Healthcare) was added on the mask and hard vacuum was applied for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 μl 0.5 mM NiCl2 ...
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bioRxiv - Genetics 2022Quote: ... 2 ul of Streptavidin Sepharose High Performance beads (GE Healthcare) and 35 ul of H2O ...