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Citations for GE Life Sciences :
2901 - 2950 of 7125 citations for Human Fibrinogen C Domain Containing Protein 1 FIBCD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Sonicated lysate was cleared by centrifugation at 12,000g for 20 minutes at 4°C and then applied to pre-washed glutathione sepharose beads (GE healthcare) overnight at 4°C with rotation ...
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bioRxiv - Cell Biology 2019Quote: HEK-293T were obtained from ATCC and cultured at 37°C and 5% CO2 in high-glucose DMEM (GE Healthcare) supplemented with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 48,284 g for 45 min at 4°C and loaded into a HisTrap FF cartridge (GE Healthcare). Proteins were eluted with 100 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... After the reaction, the sample was centrifuged briefly (10 min, 16,000g, 4°C) and loaded onto a Superdex 200 column (GE Healthcare) to remove Sfp and unreacted dsOligo-CoA ...
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bioRxiv - Biochemistry 2021Quote: ... After centrifugation at 27.000 xg for 30 min at 4 °C the supernatant was incubated with 4 mL Ni2+-Sepharose resin (GE Healthcare) for 1 hour at 4 °C under gentle agitation ...
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bioRxiv - Biochemistry 2020Quote: ... BiP and Grp94 were expressed in Escherichia coli strain BL21* at 37°C and purified via Histrap column (GE Healthcare), by ion exchange on MonoQ column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Plant Biology 2020Quote: ... An equal amount of protein was boiled in Laemmli buffer for 10 min at 95°C and separated on 12.5% acrylamide gel and blotted on a nitrocellulose membrane (GE healthcare). Blocking was done with 0.5% w/v of BSA in Tris-buffered saline with 0.1% v/v Tween 20 (TBST) ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was centrifuged for 30 min at 30.000x g at 4 °C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged proteins were eluted with a linear gradient of buffer B (50 mmol L−1 Hepes at pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... The solution was centrifuged for 30 min at 38,000 × g and 4°C to remove precipitates and applied onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB3 ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared by two rounds of 20 min centrifugation at 45,000 x g at 4°C and loaded onto a 5-mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... Insoluble material was sedimented by centrifugation at 13,500 g for 30 min at 4°C prior to loading on His-trap FF columns (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... filter and size-exclusion chromatography at 4 °C on a Superdex 200 10/300 GL gel filtration column (GE Healthcare). Purity of the proteins was evaluated on SDS-PAGE gel ...
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bioRxiv - Microbiology 2022Quote: ... SPR measurements were recorded at 20 °C using a Biacore 8k system using a ReDCaT SA sensor chip (GE Healthcare) with 8 immobilised channels as described in (43) ...
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bioRxiv - Pathology 2021Quote: ... the crude protein solution was finally collected by ultracentrifugation (40,000 rpm, 30 min, 4°C) and subsequently incubated with Ni-NTA (GE healthcare) resin for 2 h at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was dialyzed against 20 mM Tris-HCl pH 8.0, centrifuged (15 min, 48000xg, 4 °C) and loaded onto a 15 ml Q-sepharose FF (GE Healthcare) column equilibrated with the same buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plates were incubated at 37° C and imaged after 20 h with an Amersham Typhoon 5 Biomolecular Imager (GE Healthcare) using the following settings ...
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bioRxiv - Biochemistry 2019Quote: ... the concentrated protein was gel-filtrated using a Superdex 200 10/300 GL column equilibrated in HBS at a flow rate of 0.5 ml/min and 4 °C (GE Healthcare). Peak fractions at a final OD280nm of ∼ 0.4 (∼ 1.4 μM ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Biophysics 2020Quote: ... Protein fractions were dialyzed against buffer C (50 mM PB and 100 mM NaCl, pH=7.0) by desalting column (GE Healthcare), and concentrated by centrifugal filter units (Merck Amicon) ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were cleared by centrifugation at 100,000 g at 4°C for 30 min and then mixed with Glutathione Sepharose 4B (GE Healthcare). The Sepharose beads were washed with buffer B [50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were grown at 37°C with 5% CO2 in a humidified atmosphere in DMEM/HIGH glucose (Cat# SH30022.01, GE Healthcare) for SK-N-SH ...
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bioRxiv - Biochemistry 2019Quote: ... Extracts were cleared by centrifugation for 10 min at 21,000 × g at 4°C and incubated while rotating with 200 μl IgG Sepharose beads (GE Healthcare) for 4 hrs at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... L protein-containing fractions were pooled and diluted 1:1 with buffer C (20 mM HEPES(NaOH) pH 7.0) before loading on a heparin column (HiTrap Heparin HP, GE Healthcare). Proteins were eluted with Buffer A and concentrated using centrifugal filter units (Amicon Ultra ...
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bioRxiv - Biophysics 2021Quote: ... Reactions were incubated overnight at 4°C and resolved over a Superose 6 Increase 10/300 GL column (GE Healthcare) preequilibrated with assembly buffer ...
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bioRxiv - Cell Biology 2021Quote: ... were cultured at 37°C in 5% humidified CO2 in high glucose Dulbecco’s Modified Eagle Medium (DMEM, Sigma or GE Healthcare), supplemented with 4 mM L-glutamine ...
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bioRxiv - Biochemistry 2020Quote: ... L protein-containing fractions were pooled and diluted with an equal volume of buffer C (20 mM HEPES(NaOH) pH 7.0) before loading onto a heparin column (HiTrap Heparin HP, GE Healthcare). Proteins were eluted with buffer A and concentrated using centrifugal filter units (Amicon Ultra ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was dialyzed against 10 mM Tris-HCl pH 8.0, centrifuged (15 min, 48000xg, 4 °C) and passed over a Q-sepharose FF (GE Healthcare) column ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 10 mM acetic acid-NaOH pH 4.0 overnight at 4 °C and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 10 mM acetic acid-NaOH pH 4.0 ...
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bioRxiv - Biochemistry 2022Quote: Titrations were performed at 25°C with a stirring speed of 750 rpm using the MicroCalTM iTC200 System (GE Healthcare) as previously described [41] ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting supernatant was recovered and centrifuged at 10,000 xg for 10 min at 4°C and then loaded onto a HisTrap column (GE Healthcare) pre-equilibrated with the same resuspension buffer ...
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bioRxiv - Cell Biology 2022Quote: ... After centrifugation at 39,000 x g at 4 °C for 20 min the supernatant was incubated with 0.5 ml of pre-equilibrated IgG-Sepharose slurry (GE Healthcare) at 4 °C for 1 h ...
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bioRxiv - Plant Biology 2022Quote: Analytical size exclusion chromatography was performed at 4°C using a Superdex 75 10/300 gel filtration column (GE Healthcare) pre-equilibrated in 20 mM HEPES pH 7.5 and 150 mM NaCl (Maqbool et al ...
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bioRxiv - Molecular Biology 2022Quote: ... for 10 min at 4 °C and injected into Superdex 200 Increase 10/300 GL size-exclusion columns (GE Healthcare). Fractions containing both hZnT7 and Fab were collected and concentrated with a 100-kDa cutoff Amicon filter to around 10 mg ml-1 for cryo-EM measurements.
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bioRxiv - Microbiology 2023Quote: ... the viral lysate was filtered through a 1.2 µm pore size glass microfiber filter (grade GF/C, GE Healthcare Whatman) followed by a 0.45 µm pore size Nalgene Rapid-Flow filter unit (PES ...
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bioRxiv - Biophysics 2024Quote: ... The resulting suspension was then centrifuged (13,000 rpm for 35 min at 4°C) and the supernatant was applied to a HisTrap HP column (GE Healthcare) previously equilibrated with the lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0) overnight at 4°C and further purified with Resource-S cation exchange column (GE Healthcare). Finally ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 30,000 g for 30 min at 4°C and incubated for 45 min with amylose beads (GE Healthcare). The loaded protein was cleaved on column by GST-tagged 3C protease at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... AcrB was then buffer exchanged to buffer C (50 mM sodium phosphate, 150 mM NaCl, 10 % Glycerol, pH 7.4) using a PD-10 desalting column (GE Healthcare) and flash frozen at -80 °C ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4°C and purified by a gel filtration HiLoad 26/600 Superdex 200 pg column (GE Healthcare, USA) using the same buffer.
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bioRxiv - Biochemistry 2023Quote: ... and lysates were clarified (16,000 x g for 45 min at 4 °C) before being loaded onto a 5 mL HisTrap column (GE Healthcare). This was followed by one column volume (c.v. ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... and collected by centrifugation at 15,000 g for 10 min at 20 °C followed by size exclusion chromatography using a PD10 column (GE Healthcare). Importantly ...
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bioRxiv - Biophysics 2023Quote: ... CFTR was purified by gel filtration chromatography at 4 °C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with 0.006% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... The resulting suspensions were centrifuged (12.000 rpm for 35 min at 4α C) and the supernatants were purified through a HisTrap HP column (GE Healthcare) previously equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap CaptoS 5-ml column at 4° C (GE Healthcare). The column was washed with 10 vol ...
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bioRxiv - Biophysics 2021Quote: ... The protein was purified from the lysate using Ni2+-NTA column of [KTA Pure (GE healthcare). For in vitro biotinylation of the polyprotein ...
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bioRxiv - Biophysics 2020Quote: ... the protein was loaded onto a HiLoadTM 26/10 Q Sepharose high performance column (GE Healthcare), and eluted at ∼350 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... the proteins were purified by chromatography on a HiLoad 16/60 Superdex 200 column (GE Healthcare), in buffer containing 20 mM Tris-HCl ...