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Citations for GE Life Sciences :
2801 - 2850 of 7125 citations for Human Fibrinogen C Domain Containing Protein 1 FIBCD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... pre-cleared protein was loaded via injection valve onto a Sephacryl S100HR column (GE Healthcare) (26/60 ...
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bioRxiv - Microbiology 2023Quote: ... The soluble protein was loaded onto a 1.7 mL cation-exchanger column (MonoS; GE Healthcare) pre-equilibrated with buffer A50 (50 mM Tris pH 7.6 ...
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bioRxiv - Immunology 2023Quote: ... His-tagged proteins were purified from transient transfection supernatants on HisTrap HP columns (GE Healthcare) using an ÄKTAxpress (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein samples (20µl) were injected onto a Superdex 200 10/300 Increase column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein sample was passed over a HiLoad 26/600 Superdex 200 column (GE Healthcare) pre-equilibrated with the equilibration buffer (100 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The running buffer for the immobilization of the proteins was PBSP (GE Healthcare Life Sciences). The binding assay was carried out using the same buffer and a flow rate of 30 μL/min at 25 °C ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were purified by nickel affinity chromatography using Ni Sepharose 6 Fast Flow (GE Healthcare) and eluted with 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein expression was visualized with an Amersham ECL Western Blot System (GE Healthcare Life Sciences). β-Actin served as a loading control ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were subjected to anion exchange chromatography using the HiTrap™ Q column (GE Healthcare) using the ÄKTA™ start system (GE Healthcare) ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Protein bands were visualized using ECL Prime Western Blotting Detection Regent (GE Healthcare; Amersham, UK) and a light-capture cooled CCD camera system (ATTO Corp. ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins separated by 10 % Tris-glycine SDS–PAGE were transferred to nitrocellulose membranes (GE Healthcare) and processed for immunoblotting using the ECL Prime kit (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified using Glutathione Sepharose™ 4 Fast Flow (GE Healthcare #17-5132-03) and cleaved using 3C protease protein prior to reverse purification using glutathione beads followed by a HiLoad 16/60 Superdex 75 size exclusion chromatography.
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bioRxiv - Biochemistry 2024Quote: ... the proteins were purified with a Hiload 16/600 Superdex 200 pg column (GE Healthcare) preequilibrated with storage buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein expression levels were visualized with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences).
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bioRxiv - Biochemistry 2024Quote: ... the protein elution was further purified by Superdex 200 Increase 10/300 GL (GE Healthcare).
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bioRxiv - Genetics 2024Quote: ... Proteins were detected by chemiluminescence using ECL SelectTM Western blotting Detection reagent (GE Healthcare, AmershamTM) and a Fujifilm LAS-3000 camera.
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 mm NC) at 90V for 90 min on ice in transfer buffer (48mM Tris–HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Protein bands were visualized and imaged by an ImageQuant LAS 500 scanner (GE Healthcare, USA). After transfer ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were purified from buffy coats by two gradient centrifugations: PBMCs were initially isolated with Ficoll-Paque (GE Healthcare, Pittsburgh, PA) and subsequently peripheral monocytes were isolated with Percoll (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: Human HeLa (ATCC® CCL-2™) were cultured in DMEM (PanEco) supplemented with 10% fetal bovine serum (FBS; HyClone/GE Healthcare) and penicillin/streptomycin ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were isolated from heparinised blood and tissue derived mononuclear cells were isolated from mechanically dissociated and filtered human tissue using Ficoll density gradient centrifugation (Ficoll PaquePlus; GE Healthcare, Amersham) or 33% Percoll (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: FH was isolated from human plasma using an affinity column made up of a monoclonal antibody against human FH (MRCOX23; MRC Immunochemistry Unit, University of Oxford) coupled to CNBr-activated Sepharose (GE Healthcare, UK) as described previously (21) ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2023Quote: Binding of mAbs to human FcRn was performed by measuring surface plasmon resonance (SPR) using a Biacore T200 instrument (GE Healthcare, UK), as described previously (42).
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bioRxiv - Cell Biology 2020Quote: ... Time-lapse imaging was carried out at 27 °C in an Environmental Chamber with a DeltaVision Spectris (Applied Precision) comprising an Olympus IX70 widefield inverted epifluorescence microscope ...
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bioRxiv - Neuroscience 2021Quote: ... They were maintained within 37°C incubators at 5% CO2 in DMEM + high glucose (GE Healthcare Bio-Science, SH30022FS) supplemented with 9.09% fetal bovine serum (50 mL added to 500 mL DMEM ...
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bioRxiv - Genomics 2019Quote: ... hybridization (at 68°C in 50% formamide) and washing was done following the instructions of the manufacturer (GE Healthcare). The riboprobes were obtained by rub-off transcriptions of linearized pBlueScript plasmids containing the full retrozymes in the presence of DIG-UTP (Roche Diagnostic GmbH ...
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bioRxiv - Developmental Biology 2021Quote: ... dried for one hour at 80°C and exposed overnight to Phosphor screen in a cassette (GE healthcare, USA). Images were captured in Fujifilm FLA 5100 scanner (Japan ...
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bioRxiv - Biochemistry 2020Quote: ... Analytical size exclusion chromatography was performed at 24 °C using a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with a mobile phase containing 10 mM HEPES (pH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... Two 2 ml aliquots were collected using GF/C glass microfiber filters (20 mm; Whatman, GE Healthcare Life Sciences), transferred to glass tubes ...
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bioRxiv - Biophysics 2022Quote: ... overnight at 4°C then purified over a Sephadex 200 Increase 10/300 (GE Healthcare Life Sciences, Pittsburgh, PA) size exclusion column in Buffer A with 0.02% DDM.
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bioRxiv - Biophysics 2022Quote: ... The mixture was incubated at 10°C for 3h and then loaded onto a His Spintrap column (GE Healthcare). After 1-hour incubation ...
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bioRxiv - Cell Biology 2022Quote: Living embryos were imaged at 21°C using a DeltaVision OMX V3 Blaze microscope (GE Healthcare Life Sciences, UK). The system was equipped with a 60x/1.42 NA oil UPlanSApo objective (Olympus Corp.) ...
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bioRxiv - Biophysics 2019Quote: ... its only cysteine at amino acid #265 near the flexible C terminus was labeled with Cy5 maleimide (GE healthcare) or AlexaFluor488 C5 maleimide (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was then incubated with increasing amounts of ribosomal subunits/ribosomes for 15 min at 37°C before being rapidly filtrated through a cellulose nitrate membrane filter (0.2μm, GE Healthcare). The cellulose nitrate membranes were washed with 1 mL of cold TAKM5 buffer and placed into 10 mL of EcoLite (+ ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission, GE Healthcare). Fusion protease was removed using glutathione resin and the cleaved β2HA was collected in the flow-through ...
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bioRxiv - Immunology 2020Quote: ... culture media was collected and incubated overnight at 4°C with nickel-Sepharose Excel (GE Healthcare 17-3712-01). His-tagged proteins were eluted from the nickel-Sepharose with 1 M imidazole in TrisCl pH 8.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli BL21 SI pRARE at 18 °C overnight and purified using a HisTrap FF 5-ml column (GE Healthcare) in standard buffers (50 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 2 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Plant Biology 2024Quote: Analytical SEC experiments were carried out at 4 °C using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in SEC running buffer ...
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bioRxiv - Biochemistry 2024Quote: ... All purification steps were performed at 4°C and the pre-packed amylose column (5ml Hi-Trap GE healthcare) was equilibrated with 25 mL Amylose column A Buffer ...
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bioRxiv - Immunology 2022Quote: The IgG antibody or Fab binding properties were analyzed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... were run on 8% polyacrylamide gel and electrophoretically transferred onto nitrocellulose membrane (RPN303E, Hybond-C-extra, GE Healthcare Europe). The membranes were incubated at 4 °C under gentle shaking for 1 h in blocking buffer (1× TBS ...
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bioRxiv - Biophysics 2023Quote: ... Data collection was carried out at 20 °C using a Superdex200 Increase 5/150 analytical SEC column (GE Healthcare) equilibrated in the appropriate buffers (see Table S3 ...
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bioRxiv - Bioengineering 2024Quote: ... 10% glycerol (v/v) at 4°C and re-loaded onto a Ni-NTA column (HisTrapTM HP, GE Healthcare). An imidazole gradient was applied (from 20mM to 500mM ...
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bioRxiv - Microbiology 2024Quote: ... The complex was resolved by gel filtration at 4 °C with a Superdex 200 10/300 column (GE Healthcare) equilibrated with 75 mM KCl ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were dried under vacuum at 75°C onto 3MM paper (Whatman) and imaged using Typhoon phosphorimager (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... The fractions containing pure OmpLA were pooled and further passed through a 1ml HiTrap Q-Sepharose fast flow (GE healthcare) anion-exchange column equilibrated with the same buffer ...
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bioRxiv - Biochemistry 2019Quote: ... and buffer exchanged to 10 mM phosphate buffer pH 7.4 containing 10 mM MgCl2 using 10 kD Vivaspin 20 ml columns (GE Healthcare). Samples were then loaded onto a CHT ceramic hydroxyapatite column (Bio-Rad Laboratories Inc.) ...