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Citations for GE Life Sciences :
2851 - 2887 of 2887 citations for Tubulin Delta 1 TUBD1 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Fifty seeds per replicate were sown in Petri dishes containing three layers of filter paper (Whatman grade 1, GE healthcare, Little Chalfont, United Kingdom) and imbibed with 4 mL of ultrapure water (UPW) ...
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bioRxiv - Neuroscience 2020Quote: ... installed prone on the MRI bed and anaesthesia was immediately switched to 1% ISO in medical air through a respirator (Aestiva, GE Healthcare, Datex-Ohmeda, USA). The respirator allowed continuous control of respiration rates ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified using a NiNTA purification system (HisTrap™ excel 1 mL) and size-exclusion chromatography using HiPrep™ 26/60 Sephacryl™ S-100 HR (GE Healthcare) in 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: A paper punch was used to make 3 mm diameter circles of Whatman™ Grade 1 180 μm thick cellulose filter paper (GE Healthcare Life Sciences), which were stored in sterile disposable petri dishes ...
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bioRxiv - Cell Biology 2019Quote: ... Selected plakoglobin-containing fractions (Fig. 1d, fractions #8-11) were combined and loaded onto a 1 ml Resource Q anion exchange column (GE Healthcare Life Sciences, UK), equilibrated with 20 mM Tris pH 7.2 and 500 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... and Gabarapl2 (EcoRI/SalI) were amplified from cDNA derived from B16F10 melanoma cells and cloned in the vector pGEX-4T-1 (GE Healthcare, Chicago, Illinois, USA) into the sites indicated in parentheses ...
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bioRxiv - Synthetic Biology 2019Quote: ... between 5 and 10 mg protein ml−1) was pipetted into the measurement cell of a MicroCal ITC200 isothermal titration calorimetry instrument (GE Healthcare Life Sciences, USA). To avoid potential further unfolding of mutant protein we directly analysed them in imidazole-containing buffer A without previous dialysis ...
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bioRxiv - Genomics 2021Quote: ... as follows: The proteinase K-treated reaction was combined with 1 μL of Sera-Mag carboxylate-modified magnetic particles (GE Healthcare, Chicago, IL, USA.) and 72 μL of binding buffer (300 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... The Fc and IdeS A were removed using a mix of Protein A Sepharose® Fast Flow (250 μL per 1 mg digested mAb; GE Healthcare Life Sciences) and Ni Sepharose™ 6 Fast Flow (50 μL per 1 mg digested mAb ...
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bioRxiv - Biochemistry 2022Quote: ... was passed through the HisTrap column previously equilibrated with IMAC buffer A at 2 ml/min flowrate with a peristaltic pump (GE Healthcare Pump P-1) and then washed with 5 CVs of buffer IMAC A supplemented with 50 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... were mixed together at a molar ratio of 1:2 and loaded on a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare, Chicago, IL, USA) pre-equilibrated with 20mM Tris-HCl (8.0) ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... DTX3L and Flag epitope for 1 h at 4°C before Protein G Sepharose beads (30 μL per reaction mixture; GE Healthcare #GE17-0618-01) were added for 12 h ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc portion and uncut IgG was removed by flowing through a 1 mL or a 5 mL MabSelect SuRe column (GE Healthcare #11-0034-95) in PBS (Fab flows through the column) ...
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bioRxiv - Immunology 2023Quote: ... and processed within 2 hours by dilution at 1:1 (v/v) ratio in phosphate-buffered saline solution (PBS) with layering on top of 1 volume of Ficoll-Paque Premium (GE Healthcare, Chicago, IL, USA) and separation by density centrifugation at 500× g for 30 minutes (20°C ...
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bioRxiv - Biochemistry 2021Quote: ... diluted to 1 mL with buffer B and added immediately onto an anion exchange resin (Q resin; GE Healthcare; 0.2 mL; equilibrated in buffer H) and incubated (5 min) ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were transferred to an activated (100% Ethanol, 1 min; followed by two washing steps with water) PVDF membrane (GE Healthcare, Buckinghamshire, UK; Cat# 10600023) at 20V for 45 min ...
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bioRxiv - Immunology 2019Quote: ... Cells were harvested and lysed in PBS supplemented with 1 mM PMSF and soluble protein was purified using nickel-affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare, Piscataway, New Jersey, USA). After SDS-PAGE analysis ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1 to 2 mL culture volume at a determined OD730 (between 8 and 10) were filtered onto pre-dried and pre-weighed glass microfiber filters (47 mm diameter, 1 µm pore size, GE Healthcare, Cat. No. 1822-047), washed twice with deionized water and dried overnight at 65 °C (until mass deviation between readings was no higher than 0.0001 g) ...
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bioRxiv - Zoology 2019Quote: ... The gDNA concentration was estimated by Qubit and about 20 ng of gDNA amplified with primers specifically designed to include the intron region (Hsp70 genomic F/R, Table 1) using PuReTaq Ready-To-Go™ PCR Beads (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Bioengineering 2019Quote: ... cells debris were removed by centrifugation at 16,000 × g for 20 min at 4 °C and the supernatant (supplemented with 20 mM Imidazole) was loaded on a 1 mL Ni+-HisTrapHP column (GE Healthcare #71-5027-68 AK) and washed with a buffer containing 100 mM Tris-HCl (pH 7) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... with 10 mM PTI and PTI alone in water (2 mL) were loaded into 2 mL mini-dialysis tubes with 1 kDa molecular weight cut-off (GE Healthcare Bio-sciences Corp. USA). The tube was immersed in a 200 mL closed Duran flask which was placed in a water bath at 37°C for the duration of the experiment ...
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bioRxiv - Molecular Biology 2019Quote: ... approximately 30 μg of total RNA was denatured and separated on a 1% denaturing formaldehyde agarose gel and transferred to a Hybond-N+ nylon membranes (GE Healthcare Life Sciences, London, UK). PCR products were labeled with DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Microbiology 2019Quote: For the biomass evaluation in the AD7 medium optimization experiment 0.4 to 1 mL of cell cultures were filtered onto pre-dried and pre-weighed glass microfiber filters (47 mm diameter, 1 µm pore size, GE Healthcare, Cat. No. 1822-047), washed three times with MilliQ water in order to remove salt remains and dried for 24 hours at 65 °C (at which point measured weight did not differ by more than ± 0.0001 g).
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bioRxiv - Biochemistry 2021Quote: ... DrrA-OCRL or DrrA-INPP5E were then loaded onto a 1 mL MonoS (5/50 GL) cation exchange column (GE Healthcare, Cat# 17-5168-01) equilibrated in 20 mM HEPES pH 7 ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mm x 1 mm x 1 mm) and FDG-PET data acquisitions were performed simultaneously on a hybrid 3 T SIGNA™ PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the Service of Nuclear Medicine (CUB Hôpital Erasme ...
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bioRxiv - Biochemistry 2022Quote: ... The cDNAs of full-length TPR-CHAT were synthesized using the bacterial expression vector pGEX-6P-1 (GE Healthcare, with an N-terminal GST tag). Point mutations were introduced using Fast Site-Directed Mutagenesis Kit in the Sb-gRAMP gene and verified by DNA sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... USA, 1:10000), luminescence was revealed with Amersham ECL™ Prime reagents (Cytiva, UK) and acquired with ImageQuant™ LAS 4000 (GE Healthcare, Il, USA).
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bioRxiv - Molecular Biology 2023Quote: ... The LacO-specific probe was end labeled with 32P-γ-ATP for 1 h at 37 °C before cleaning with illustra ProbeQuant G-50 micro column (GE Healthcare; 28-9034-08). The blot was incubated for 2 h at 42 °C in hybridization buffer (ULTRAhyb™ Ultrasensitive Hybridization Buffer [Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA product was precipitated by ethanol at −80°C and final DIG- or FLR-labelled riboprobe quality was assessed by RNA gel electrophoresis on a 1% gel and final concentration was determined by Nanodrop spectrophotometry (GE Healthcare, Cat. No. 28 9244-02).
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bioRxiv - Neuroscience 2021Quote: ... Supernatant was filtered with a 0.45 µm filter then left to equilibrate overnight with 1 mL of Glutathione Sepharose 4B affinity resin (GE Healthcare, Pittsburgh, PA, USA, #17-0756-01). Glutathione Sepharose 4B resin and bound protein was applied to a 10 mL disposable plastic column and washed thrice with 3 mL of wash buffer (GST Buffer + 0.1% Triton X-100) ...
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bioRxiv - Cell Biology 2020Quote: Plasma M- and Z-AAT was isolated by affinity chromatography using the AAT specific Alpha-1 Antitrypsin Select matrix (GE Healthcare Life Sciences, Cytiva, Sheffield, UK) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... Samples (100 µL OXAVL02 or OXAVL06 at 0.5-1 mg/mL) were loaded onto a Superdex® 200 Increase 10/300 G column (GE Healthcare Bio-Sciences AB Uppsala, SW) pre-equilibrated with the “SECMALS-PBS buffer” ...
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bioRxiv - Plant Biology 2024Quote: ... The integrity and purity of these samples were evaluated on a 1% agarose gel and on a NanoVue spectrophotometer (GE Healthcare Life Sciences, Chicago IL, US). We used an Illumina paired-end TruSeq RNA library preparation kit for 150 sequencing cycles for a mid-output flowcell ...