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Citations for GE Life Sciences :
2801 - 2850 of 2887 citations for Tubulin Delta 1 TUBD1 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...
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bioRxiv - Biophysics 2022Quote: The rat Syb2 (29–93) and Syb2 (29–93, S61C), mouse Rab3A (22–217, Q81L) were all cloned into pGEX-6p-1(GE Healthcare; Piscataway, NJ). The cytoplasmic domain of rat Syx1 (2–253 ...
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bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... The mixtures were incubated at 4°C for 1 h prior to purification on a Superdex 200 Increase SEC column (GE Healthcare Life Sciences) in buffer containing 150mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using 0.45 micrometer membranes (PES, ClearLine) and the protein purification was carried out by IMAC using 1 mL His-Trap FF columns (GE Healthcare Life Sciences) and a NGC chromatography system (Bio-Rad) ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was then vacuum filtered and loaded at 1 mL/min onto a HiTrap Q HP column (5 mL, GE Healthcare Life Sciences) pre-equilibrated with buffer D [10 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were subsequently washed with 1 x TBS/T for 30 min in 10 min intervals prior to incubation in ECLTM (GE Healthcare, cat# RPN2106) and exposure by film (FUJIFILM Super RX ...
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bioRxiv - Microbiology 2019Quote: ... Blots were UV cross-linked and baked at 80°C for 2 h prior to pre-hybridization at 42°C for 1 h in Rabid-Hyb Buffer (GE Healthcare Life Sciences). Hybridization with α-32P-ATP end-labeled DNA oligo probes was performed at 42°C for 2-16 h ...
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bioRxiv - Molecular Biology 2020Quote: ... one of which was heating in a 65 °C water bath for 60 minutes and the second was using a Hi Trap QHP column (1 mL GE Healthcare, United States). When loading ...
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bioRxiv - Microbiology 2019Quote: ... The bacterial protein PA1217 was fused to a GST-tag by cloning it into a pGEX-6P-1 vector (GE Healthcare, Chicago, IL) using the BamHI and EcoRI restriction sites (Thermo Scientific) ...
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Hypertension drives microbial translocation and shifts in the fecal microbiome of non-human primatesbioRxiv - Pathology 2021Quote: Echocardiography was performed on each of the vervet monkeys in study 1 using a GE Logiq S8 ultrasound unit (GE Healthcare, Chicago, IL) using an approach that has been previously described.20 Cardiac morphometric and functional phenotypes include ...
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bioRxiv - Developmental Biology 2021Quote: ... at a concentration of 1 × 106 spermatozoa/mL was used following separation of spermatozoa over a discontinuous Percoll gradient (45% and 90%; (GE Healthcare, Chicago, US)) ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified by affinity chromatography with nickel affinity columns (HisTrap 1 ml; Cytiva) using the Äkta pure system (GE Healthcare UK Ltd.). Elution was carried out at a flow rate of 1 ml/min with all solutions maintained at 4 °C and a linear imidazole concentration gradient from 30 mM to 530 mM.
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bioRxiv - Neuroscience 2021Quote: ... plasma samples were centrifuged and 1 μL of each sample was placed onto a clean piece of nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, USA) and left to dry out ...
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bioRxiv - Cell Biology 2020Quote: ... and WAVE2-WCA (419-497) were amplified from plasmid DNA and inserted into BamHI/SalI sites of pGEX-6P-1 (GE Healthcare, Munich, Germany). Plasmids pGEX-6P-2-cortactin (Lai et al. ...
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bioRxiv - Biophysics 2021Quote: ... was achieved through incubation at RT for 1 h and subsequent size-exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare, Pittsburgh, USA), equilibrated in storage buffer (50 mM HEPES pH 7.25 ...
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bioRxiv - Immunology 2020Quote: Purified CoV-S trimers were concentrated to 1 mg/mL and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and passed through a Wyatt DAWN coupled to a Wyatt UT-rEX differential refractive index detector (Wyatt Technology) ...
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bioRxiv - Neuroscience 2022Quote: ... installed prone on the MRI bed (Fig 1) and anaesthesia was immediately switched to 1.5-2% isoflurane (28) in medical air through a respirator (Aestiva, GE Healthcare, Datex-Ohmeda, USA). The respirator allowed continuous control of respiration rates ...
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bioRxiv - Biochemistry 2022Quote: ... 45 μl of the protein at 4.43 mg ml−1 (70.4 µM) was injected in SEC column S200i 5/150 (GE Life Sciences, Velizy-Villacoublay, France) with a buffer containing 20mM Hepes pH 7,5 ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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bioRxiv - Microbiology 2022Quote: ... High performance liquid chromatography (HPLC) was conducted using a HiTrap Q XL 1 mL Sepharose column on the ÄKTA pure protein purification system (GE Healthcare, Uppsala, Sweden). Proteins were eluted with starting buffer A followed by a gradually increasing concentration of buffer B (20 mM Bis-Tris ...
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bioRxiv - Plant Biology 2022Quote: ... Acetonenitrile was then added to 70 % and a 10:1 ratio of magnetic beads (Hydrophilic-Part no: 45152105050250, GE Healthcare plus Hydrophobic-Part no: 65152105050250, GE Healthcare, Australia) were added and mixed with the solution ...
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bioRxiv - Cell Biology 2024Quote: ... This was injected into a size-exclusion column (column size 24 cm x 1 cm) containing Sepharose 4B (84 nm pore size) using an AKTA start system (GE Healthcare Life Science) and eluted with PBS ...
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bioRxiv - Microbiology 2022Quote: ... brasiliensis by co-cultivation with yeasts at a ratio of 1:10 in sterile Hybond N filters (GE Healthcare Life Science, Pittsburgh, PA) in IM solid medium (induction medium ...
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bioRxiv - Molecular Biology 2022Quote: ... the samples were incubated at 4°C for at least 1 hr with ∼40 µL of appropriate bead slurry (nProtein A Sepharose, GE Healthcare, 5280-01). Following incubation ...
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bioRxiv - Bioengineering 2024Quote: ... 2 μg DNA loaded per lane was separated by electrophoresis on 1% agarose gels and transferred to Hybond-N membranes (GE Healthcare, Chicago, IL) using capillary flow transfer method ...
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bioRxiv - Immunology 2024Quote: ... 1:3 blood dilutions were made with sterile Roswell Park Memorial Institute (RPMI) 1640 medium (HyClone; GE Healthcare Life Sciences, Chicago, IL, USA) supplemented with 100 unit/mL penicillin and 100 mg/mL streptomycin (Gibco Life Technologies ...
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Rhodococcus equi Escapes Macrophage Autophagy: VapA Inhibits ATP6V0d1-Mediated Lysosomal DegradationbioRxiv - Immunology 2023Quote: ... second antibody (1:10000) for 1 h at room temperature and then proteins were visualized with enhance chemiluminescence (ECL) kit purchased from GE Healthcare (Chicago, USA). And detection reagent and analyzed using GE Amersham Imager 600.
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bioRxiv - Microbiology 2023Quote: ... probed with a 1:2,000 dilution of polyclonal α-PotA (Lin et al., 2017) followed by a 1:10000 dilution of peroxidase labeled α-mouse (GE Healthcare) and developed with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... NRP was measured by first spotting 1 μl of each sample on the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, USA). Dry membrane was immersed in NRP working solution (10mg/ml KMnO4 in ddH2O) ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and with KCl to a final concentration of 0.3 M and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... This oligo was conjugated with succinimidyl 4-(N-maleimidomethyl)cyclohexane-1- carboxylate (SMCC linker through NH2-NHS ester reaction. The SMCC-linked oligo was purified through Nap column (GE Healthcare Life Sciences). The purified SMCC-linked oligo was conjugated with OVA through cysteine (thiol)-maleimide group coupling ...
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bioRxiv - Microbiology 2023Quote: ... The 35 mer DNA oligonucleotide probes were radiolabelled with 32P-ATP at 37°C for 1 hour and then purified over a G50 size exclusion column (GE Healthcare Life Sciences) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Antibodies were purified from filtered culture supernatants by fast protein liquid chromatography (FPLC) on an ÄKTA Pure instrument using a 1 mL HiTrap MabSelect PrismA column (Cytiva, formerly GE Healthcare Life Sciences). Purified mAbs were concentrated and buffer changed into PBS using Amicon® Ultra-4 50KDa Centrifugal Filter Units (Millipore Sigma) ...
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bioRxiv - Genomics 2023Quote: ... was used at 1:5000.) Blots were incubated with chemiluminescent detection reagent (Amersham ECL™ Prime Western Blotting Detection Reagents, GE Healthcare #RPN2236) and imaged on an Amersham Imager 600 (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: we incubated samples for 10 min with Buffer A (1 M GA, 80% ACN, 5% TFA) followed by incubation with the TiO2 Mag Sepharose (GE Healthcare Life Sciences) for 30 min in an Eppendorf Thermomixer at 800 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... was then incubated for 1 hour at 4°C on a rotating platform with 2 mL Sepharose 2B resin (GE Healthcare Life Sciences) suspended in 2 mL kinase buffer (50 mM Tris ...
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bioRxiv - Bioengineering 2023Quote: ... Released mesh probes were rinsed with deionized (DI) water three times and transferred to sterile 1× PBS solution (HyClone, GE Healthcare Life Sciences) before surface modification or implantation.
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bioRxiv - Molecular Biology 2024Quote: ... Digested samples were passed through pre-washed (4x with 700 μl polysome buffer for 1 min at 450 x g) Sephacryl S-400 HR spin columns (GE healthcare, currently Cytiva), and centrifuged for 2 min at 450 x g at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... NHR2 was then purified by S-75 size exclusion chromatography (SEC buffer-50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT, pH 8) (GE Healthcare Life Sciences) and analyzed using SDS-PAGE ...
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bioRxiv - Plant Biology 2019Quote: ... The fusion protein was isolated from soluble cell lysate by Co-NTA affinity chromatography with two His-Trap 1 mL TALON Crude columns (GE Healthcare Life Sciences, 28953766). Chromatography buffers contained sodium phosphate 20 mM pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Whole genome DNA was amplified using 1 μl polymerase enzyme from the illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Cat# 25-6600-32) and 9 μl of sample with the following conditions ...
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bioRxiv - Immunology 2021Quote: ... The Fc and IdeS A were removed using a mix of Protein A Sepharose® Fast Flow (250 µL per 1 mg digested mAb; GE Healthcare Life Sciences) and Ni Sepharose™ 6 Fast Flow (50 µL per 1 mg digested mAb ...
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bioRxiv - Microbiology 2019Quote: ... The filtrate was diluted to 500 µl with running buffer A (50 mM Tris, pH 9) and loaded on a 1 ml Resource™ Q anion exchange column (GE Healthcare Life Sciences). A linear gradient to 40% running buffer B (50 mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... fused to triple epitope tags were prepared by directionally cloning in-frame epitope tags into the BamHI and EcoRI restriction sites of pGEX-4T-1 (GE Healthcare Life Sciences #28954549). PCR amplicons were prepared from a PAGE-purified oligonucleotide template with forward primers containing a BglII restriction site and reverse primers containing HindIII–BamHI–EcoRI sites (Supplementary Table S1) ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mL fractions corresponding to ∼10 mL of the elution peak were pooled and applied to a 1 mL StrepTrap column (GE Healthcare, #28-9075-46) pre-equilibrated with Buffer N with 2 mM DTT ...
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bioRxiv - Plant Biology 2020Quote: ... A peroxidase-conjugated secondary antibody was used at a dilution of 1:200 and immunoreactive bands were visualized with ECL Western Blotting Detection Reagents (GE Healthcare, USA; https://www.gelifesciences.com). Rubisco protein content was quantified from SDS-page gels using ALPHA-Ease FC software (Alpha Innotech ...
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bioRxiv - Biochemistry 2021Quote: Three microliters of S-D614G in 1.2 mg/ml was mixed with 1.2 equivalent of RBD-chAb45 and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mm × 1 mm × 1 mm) and brain FDG-PET were acquired simultaneously on a hybrid 3 T SIGNA PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the CUB Hôpital Erasme (Brussels ...