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Citations for GE Life Sciences :
2651 - 2700 of 4188 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plates were then sealed and imaged on the IN Cell 2500 HS (GE Healthcare, Chicago, IL) automated high-content imaging system ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were grown in HyClone Dulbecco’s Modified Eagle Medium with high glucose (GE Healthcare Life Sciences) supplemented with L-Glutamine (WISENT Inc) ...
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bioRxiv - Neuroscience 2019Quote: ... peripheral blood mononuclear cells were isolated using density gradient centrifugation on Ficoll-Paque™ (GE Healthcare). CD14+ cells were obtained using the MACS isolation columns according to the manufacturer’s protocol (Miltenyi) ...
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bioRxiv - Cell Biology 2019Quote: Immunostaining of the cells 97 was performed in 96-well glass bottom plates (Whatman, GE Healthcare, UK ...
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bioRxiv - Molecular Biology 2020Quote: All live cell imaging was carried out on a DeltaVision OMXTM V4 imaging system (GE Healthcare), equipped with a 60x (NA 1.42 ...
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bioRxiv - Immunology 2020Quote: ... the cell culture supernatants were harvested and purified by Ni-NTA affinity chromatography (GE Healthcare, USA). Similar to the preparation of rEBOVGP ...
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bioRxiv - Cancer Biology 2019Quote: ... media was removed and cells were washed 2X and then imaged by DeltaVision workstation (Applied Precision).
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bioRxiv - Genetics 2020Quote: Marmoset cell nuclei were imaged with an OMX BLAZE 3D-Structured Illumination Microscope (Applied Precision, GE). Cells were transferred to a modified stage adaptor held at 37°C and 5% CO2 ...
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bioRxiv - Genomics 2020Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density centrifugation using Ficoll-Paque PLUS (GE Healthcare) and cryopreserved in CryoStor (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The cleared cell lysates were incubated overnight with glutathione-Sepharose 4B beads (GE Healthcare Life Sciences) in the presence of 5 mM dithiothreitol (DTT ...
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bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated using a Ficoll-Paque Premium density gradient (GE Healthcare), and washed with R-10 media ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were cultured in RPMI1640 medium (Cellgro) supplemented with 10% bovine growth serum (GE Healthcare, SH30541.03) and 1% penicillin-streptomycin (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines were maintained in HyClone™ Dulbecco’s High Glucose Modified Eagles Medium (DMEM, GE Healthcare), HyClone™ McCoy’s 5A (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Mononuclear cells from bone marrow samples were isolated using Ficoll-Paque (GE Healthcare, Chicago, IL, USA) density gradient centrifugation ...
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bioRxiv - Bioengineering 2020Quote: ... and imaged by a GE IN Cell Analyzer 2000 imaging system (GE Healthcare, Chicago, IL, USA). For the staining ...
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bioRxiv - Immunology 2020Quote: ... The proteins were purified from the cell lysate using 300 μl GST resin (GS4B, GE Healthcare), and incubated with excess purified wild-type or mutant rDPP9 proteins on ice for 60 min ...
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bioRxiv - Immunology 2020Quote: ... Cell suspension was then pelleted and then re-suspended in 4 mL 40% Percoll (GE Healthcare), layered on 4 mL 70% Percoll and centrifuged at 800xg (RT ...
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bioRxiv - Immunology 2019Quote: ... Single-cell suspensions were prepared and centrifuged over a 30%/70% discontinuous Percoll gradient (GE Healthcare) and mononuclear cells were isolated from the interface ...
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bioRxiv - Plant Biology 2022Quote: ... the cell lysate was incubated with Ni-NTA beads (Ni Sepharose 6 Fast flow, GE Healthcare) for 45 mins ...
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bioRxiv - Cancer Biology 2022Quote: Images of mitotic cells and metaphase spreads were acquired on Deltavision Elite imaging system (GE Healthcare) equipped with a Cool SnapHQ2 charge-couple device (CCD ...
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bioRxiv - Developmental Biology 2022Quote: ... the cell suspension was loaded on a Percoll (GE Healthcare, Chicago, IL, cat#: 17-0891-01,) gradient of 35% and 65% and centrifuged at 1800×g for 10min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Mononuclear cells were isolated at the interphase of a 30%–70% Percoll gradient (GE Healthcare P1644). Splenocytes from were isolated and homogenized through a 70 μm cell strainer ...
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bioRxiv - Plant Biology 2022Quote: ... The clarified cell lysate was first injected onto a 5 ml Ni2+-NTA column (GE Healthcare). The complexes were step-eluted with elution buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell-free extract was loaded onto NiCl2 charged fast-flow chelating sepharose column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were purified from cell extracts using MBPTrap™ HP column (GE Healthcare, Chicago, IL, USA) with ÄKTA FPLC system (Amersham Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... the cell-free extract was loaded onto NiCl2 charged-fast-flow-chelating-sepharose column (GE Healthcare) which was pre-equilibrated with buffer (20mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1000 μg of cell lysates were incubated overnight with Glutathione Sepharose™ beads (GE Healthcare, UK) at 4 °C on a rotator ...
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bioRxiv - Genomics 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated according to a standard Ficoll-Paque plus (GE healthcare) protocol ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation over Ficoll-Paque (GE Healthcare) and cryopreserved until use ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant of the cell lysate was loaded onto a HisTrap column (5 mL-GE Healthcare) and the bound protein was eluted with an imidazole gradient (0-1 M) ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble cell lysates were applied on a Ni-sepharose excel (GE Healthcare, #17-3712-01), packed in a column of 4 ml bed volume with Sl1 buffer buffer (20mM Tris ...
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bioRxiv - Genomics 2023Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation over Ficoll-Paque (GE Healthcare) followed by multiple washes in PBS/EDTA (Miltenyi Biotec) ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates prepared by sonication and centrifugation were purified on a HisTrap HP column (GE Healthcare). The N-terminal polyhistidine-GST tag was cleaved by incubation with TEV protease at 4 °C overnight ...
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bioRxiv - Biophysics 2023Quote: ... clarified lysate obtained from the cells was bound to a HiTrap Chelating HP column (GE Healthcare) in low imidazole buffer (50 mM PIPES ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were grown overnight at 37°C on nitrocellulose membranes (Amersham™ Protran™, GE Healthcare) in plates containing ZYP agar medium (supplemented with 100 µg.mL-1 ampicillin and 34 µg.mL-1 chloramphenicol) ...
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bioRxiv - Immunology 2023Quote: ... Hepatocytes were separated from immune cells by a 35% and 70% bilayer Percoll gradient (GE Healthcare) and used.
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMC) were isolated from whole blood by Ficoll-Hypaque centrifugation (GE Healthcare) and processed according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: HTB2-mCherry expressing cells were imaged at room temperature using a DeltaVision (pDV) microscope (Applied Precision) equipped with a CoolSNAP HQ2/HQ2-ICX285 camera using a 60× oil objective (U-Plan S-Apochromat-N ...
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bioRxiv - Biochemistry 2023Quote: ... The cell extract was applied onto a HisTrap™ FF crude column (5 ml; GE Healthcare) pre-equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation (Ficoll-Histopaque; GE Life Sciences) from blood collected in ACD tubes ...
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 20.000 alive/mCD45-/hCD45+/hCD19+ cells were sorted into a PBS (GE Healthcare Life Sciences) + 2% FBS (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... when cells were transfected with either 100 nmol/L siRNA targeting FADS1 (siFADS1, GE Healthcare Dharmacon), or the respective negative controls (non-targeting small interfering RNA ...
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bioRxiv - Microbiology 2023Quote: ... Cells were viewed at room temperature with a DeltaVision Core microscope system (Applied Precision/GE Healthcare). Seven planes were acquired every 200 nm and the data were deconvolved using SoftWorx software as described previously (66) ...
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bioRxiv - Systems Biology 2023Quote: ... Time-lapse microscopy images were acquired using an IN Cell Analyzer 2500HS (GE Healthcare Life Science) with a CFI S Plan Fluor ELWD dry objective lens 20× (NA ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were subsequently imaged for 8 hours on a DeltaVision Elite imaging station (GE Healthcare), equipped with a CoolSNAP HQ2 camera ...
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bioRxiv - Neuroscience 2023Quote: ... Single cell suspensions were prepared and centrifuged over a 37%/70% discontinuous Percoll gradient (GE Healthcare), mononuclear cells were isolated from the interface ...
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Image acquisition was done with the InCell Analyzer 6000 Cell Imaging System (GE Healthcare Life Sciences) and micrographs were assembled using Adobe Photoshop 2022.
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bioRxiv - Molecular Biology 2023Quote: ... The viability was quantified by staining the yeast cells in 0.1% Trypan blue staining (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density centrifugation using Ficoll-Paque Plus (GE Healthcare). Plasmacytoid dendritic cells and monocytes were isolated using anti-BDCA4 or anti-CD14 magnetic microbeads (Miltenyi Biotec ...