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Citations for GE Life Sciences :
2501 - 2550 of 4264 citations for Recombinant Human DKK3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare), followed by a TEV protease cleavage at 16°C overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins were further purified by size exclusion chromatography on a Superdex200 16/60 column (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... The protein was purified using gel filtration (Superdex75 or 200 Hi-load column, GE Healthcare, Chicago, IL). The peak fractions were pooled and concentrated using filters of appropriate cut-offs (EMD Millipore ...
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bioRxiv - Immunology 2021Quote: ... Rhesus sera or plasma antibodies were first purified by affinity chromatography using Protein A Sepharose (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The approximate molecular weights of the loaded proteins were determined by molecular weight calibration kits (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... the protein was concentrated and further purified by gel filtration using a superpose 6 increase (GE healthcare) in TBS containing 0.01% LMNG ...
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bioRxiv - Bioengineering 2021Quote: ... The protein was further purified by size-exclusion chromatography using a HiLoad Superdex 200PG column (GE Healthcare) with PBS as an eluent ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were purified by immobilized metal-affinity chromatography (IMAC) using pre-packed nickel Sepharose columns (GE Healthcare), or in batch mode on TALON® beads (Takara Bio ...
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bioRxiv - Immunology 2020Quote: ... and the protein was separated on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare) equilibrated with TBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved by 10% SDS PAGE and transferred on a nitrocellulose membrane (Protran BA83, GE Healthcare) using a semi-dry system (Bio-Rad Laboratories S.r.l.) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were either stained with Coomassie brilliant blue G-250 or transferred to nitrocellulose membranes (GE Healthcare). The membranes were blocked with 5% milk in 0.05% Tween TBS at room temperature for 1 h and then incubated at 4 °C overnight with a specific polyclonal serum produced by immunization of mice with RBD produced in HEK-293T cells ...
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bioRxiv - Biochemistry 2020Quote: ... the proteins were buffer exchanged into the appropriate buffer using an Illustra NAP-5 column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then loaded on a Superdex 200 10/300 GL Tricorn gel filtration column (GE Healthcare), and eluted with 1.5 column volumes of gel filtration buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... the eluted protein was loaded onto a size exclusion column (Superdex 200 16/600 column, GE Healthcare) and eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Concentrated protein was loaded onto a 16/600 Superdex 200 gel filtration column (GE Healthcare Piscataway, NJ). The buffer conditions were the same as in Ni2+-NTA affinity chromatography but with 20 mM NaCl and no imidazole ...
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bioRxiv - Neuroscience 2022Quote: ... The Twin-Strep-tagged protein was purified via affinity chromatography using a StrepTrap HP column (GE Healthcare). Next ...
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bioRxiv - Microbiology 2022Quote: ... Periplasmic proteins were extracted and CusF was purified on a 5 ml StrepTrap HP column (GE healthcare) equilibrated with buffer A (10 mM NaPi ...
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bioRxiv - Microbiology 2022Quote: ... proteins were eluted and further purified by size-exclusion chromatography on Superdex 75 (GE Healthcare Life Sciences). Purified DksA proteins were aliquoted inside a BACTRON anaerobic chamber (Shel Lab ...
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bioRxiv - Biochemistry 2022Quote: ... thrombin or PreScission protease and proteins were further purified by ion exchange chromatography (Source 15Q, GE Healthcare) and gel filtration chromatography (Superdex 200 ...
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bioRxiv - Microbiology 2019Quote: ... Untagged Orn proteins were recovered in the flow-through of a HisTrap Ni-NTA column (GE Healthcare), separated from His6-SUMO ...
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bioRxiv - Pathology 2020Quote: ... The presence of P1 proteins was revealed using the ECL Plus Western Blotting Detection System (GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... the eluted protein was loaded onto a size exclusion column (Superdex 200 16/600 column, GE Healthcare) and eluted with 20mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... where the concentrated protein sample was applied onto a Superose 6 10/300 GL column (GE Healthcare) equilibrated with SEC buffer (25 mM Tris-HCl pH 8 ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were visualized using Amersham ECL Prime Western Blotting Detection System and Amersham Hyperfilm ECL (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... resin and excess biotin was separated from the labeled protein by Superdex75 gel filtration chromatography (GE Healthcare) in 20 mM HEPES buffer pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the holo-proteins were then loaded onto a Superdex 75 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Cancer Biology 2019Quote: ... The proteins were then transferred to a 0.45-μm PVDF membrane (Amersham Hybond, GE Healthcare, München, Germany) which was blocked in 2% bovine serum albumin (Amresco ...
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bioRxiv - Molecular Biology 2019Quote: ... the eluted protein was loaded onto a size exclusion column (Superdex 200 16/600 column, GE Healthcare) and eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... a frozen protein aliquot was thawed and loaded onto a Superdex HR 75 10/300 (GE Healthcare) size exclusion column and eluted with 150 mM NaCl ...
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Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infectionbioRxiv - Cell Biology 2020Quote: ... proteins were further concentrated with filters and then purified by anion exchange chromatography on HitrapQ (GE Healthcare) and collected fractions were further loaded onto size exclusion column (Superdex 75 16/60 ...
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bioRxiv - Plant Biology 2019Quote: ... The expressed protein domains were purified via Ni2+ affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The eluted proteins were subjected to buffer change by PD10 desalting column into ITC-buffer (25 mM BisTris-HCl pH6.0 ...
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bioRxiv - Microbiology 2019Quote: ... Dual-labeled capsid proteins were separated using a PD Mini-Trap G-25 desalting column (GE healthcare) with imaging buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... Size-exclusion chromatography was performed by loading the protein onto Superose 6 Increase 10/300GL (GE Healthcare) column with running buffer containing 20 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant containing soluble proteins was collected and allowed to flow through Ni-NTA resin (GE Healthcare). After washing with three column volumes of sonification buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The cleaved protein was purified further using a Superdex Increase 75 10/300 (GE Healthcare Life Sciences), pre-equilibrated with 20 mM Hepes pH 7.5 and 150 mM NaCl.
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bioRxiv - Biophysics 2020Quote: ... The plexin protein in the supernatant was captured using a 1 mL HisTrap FF column (GE Healthcare) and eluted by Buffer B containing 10 mM Tris (pH 8.0) ...
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bioRxiv - Plant Biology 2020Quote: The GST- and MBP-fusion proteins were separately purified using Glutathione sepharose (GE Healthcare, 17-5132-01) and Amylose resin (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged proteins were immobilized on 100 μl Glutathione–Sepharose beads in 1xPBS (GE Healthcare, Freiburg, Germany) directly from E ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was buffer exchanged to remove imidazole using a 16/260 HiLoad desalting column (GE healthcare). BpsDsbA was then incubated with TEV protease in a 1:50 (TEV:BpsDsbA ...
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bioRxiv - Molecular Biology 2021Quote: ... SEC was carried out by loading the protein sample to Superdex 200 Increase 10/300GL (GE Healthcare) to obtain the monomer complex ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was loaded onto a 1 ml HiTrap Heparin HP column (GE Healthcare, 17-0406-01) and eluted with a linear gradient from buffer A (20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were purified using Affinity chromatography purification on Glutathione Sepharose 4B beads affinity column (GE Healthcare). After that ...
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bioRxiv - Microbiology 2021Quote: ... the proteins were subjected to a size exclusion chromatography using a Superdex 200 Increase column (GE Healthcare) with a flow rate of 0.5 ml/min using buffer B ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 200 (GE Healthcare) column using buffer III and a flowrate of 1 mL/min ...
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bioRxiv - Biochemistry 2021Quote: ... All columns were calibrated using protein standards from both high and low molecular weight kits (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... Eluted protein was further purified using an anion exchange column (HiTrap Q HP 5 ml; GE Healthcare) before buffer exchange into PBS and storage at –20°C.
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were diluted to 75 mM NaCl and loaded onto a MonoQ 5/50 GL (GE Healthcare) equilibrated in QO buffer (20 mM Tris pH 8 ...
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Adaptation of the Romanomermis culicivorax CCA-adding enzyme to miniaturized armless tRNA substratesbioRxiv - Molecular Biology 2020Quote: ... Protein-containing fractions were combined and concentrated on Vivaspin 6 columns (15-30 kDa MWCO, GE Healthcare). Proteins were stored in 40 % glycerol (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... Binding of GAPDH proteins to EV surface was monitored by UV-280 chromatogram (Akta pure, GE Healthcare), western blotting ...