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Citations for GE Life Sciences :
2301 - 2350 of 4264 citations for Recombinant Human DKK3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before88 ...
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bioRxiv - Physiology 2022Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred onto nitrocellulose membranes (GE Healthcare). The nitrocellulose membranes were blocked with 5% BSA in TBST (TBS containing 0.1% Tween20 ...
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bioRxiv - Microbiology 2022Quote: ... M28 IgG was then purified from the supernatant using Protein A affinity chromatography (GE Healthcare #17549851). IgG was then dialyzed overnight in TBS (150mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... Quantifications of protein levels were performed with an ImageQuant™ LAS 4000mini Biomolecular Imager (GE Healthcare), and images were analyzed with ImageJ software (NIH) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein constructs were concentrated with Vivaspin 20 5000molecular weight cut-off centrifuge tubes (GE Healthcare) and stored at -80 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified using the His GraviTrap column (GE Healthcare Life Sciences, 11-0033-99) by following the manufacture’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were purified from cell extracts using MBPTrap™ HP column (GE Healthcare, Chicago, IL, USA) with ÄKTA FPLC system (Amersham Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... proteins were electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 90 V for 90 minutes on ice in transfer buffer (48 mM Tris base and 39 mM glycine supplemented with 20% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The protein was further purified using a Superdex 75 26/60 gel filtration column (GE healthcare) preequilibrated with buffer C (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Physiology 2022Quote: ... All proteins were further polished with Superdex 200 Increase 10/300 GL (GE Healthcare Life Sciences) size-exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were loaded on a 5 ml HisTrap™HP column (1 mL/min; GE Healthcare) in a binding buffer containing 100 mM tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was fitered and protein purification was conducted using a Resource Q column (GE Healthcare) equilibrated in Buffer A ...
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bioRxiv - Cell Biology 2024Quote: ... Labelled protein bands were detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, USA) and ChemiDoc MP Imaging System (Bio-Rad ...
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bioRxiv - Synthetic Biology 2023Quote: ... All proteins were further purified with Superdex 200 Increase 10/300 GL (GE Healthcare Life Sciences) size-exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2024Quote: ... the protein was purified by size exclusion chromatography using a Sephadex 200 16/60 (GE Healthcare) column with the lysis buffer.
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bioRxiv - Biophysics 2024Quote: ... A calibration curve was produced for eluting species using known protein standards (GE Healthcare Life Sciences). The dimerisation-impaired D123P construct of Ire1-LD was used to assign the apparent molecular weight of eluted monomeric protein ...
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bioRxiv - Biophysics 2024Quote: ... The protein sample was passed through His Trap™ FF crude column (GE Healthcare, Piscataway, NJ). We expected the undigested protein to remain bound to the column ...
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bioRxiv - Microbiology 2024Quote: ... and the surface proteins were bound to streptavidin Sepharose beads overnight at 4°C (GE Healthcare). Beads were then washed with buffer containing 100 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... and the proteins were then transferred onto nitrocellulose blotting membranes (NC) (10600001; GE Healthcare Life Science). The membranes were blocked with 5% non-fat dry milk and 0.1% Tween 20 for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... GST or the GST fusion protein was purified using a glutathione Sepharose 4B column (GE Healthcare). GFP::RAB-5 protein from wild-type or usp-50(xd413 ...
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bioRxiv - Developmental Biology 2024Quote: ... The overexpressed proteins were bound to the washed Ni2+-chelating Sepharose (GE Healthcare, Pittsburgh, PA, USA). The suspension was mixed on a three-dimensional rotating mixer for 40 min at 4 °C ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... proteins were electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 90 V for 90 min on ice in transfer buffer (48 mM Tris and 39 mM glycine supplemented with 20% (v/v ...
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bioRxiv - Biochemistry 2024Quote: ... The free dye was separated from labelled protein by a Hi-TRAP Desalting column (GE Healthcare). The final degree of labelling of FtsZ and ZapD was calculated from the molar absorption coefficients of the protein and the dye ...
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bioRxiv - Genetics 2024Quote: ... The proteins were electrophoresed on 10% SDS□PAGE gels and transferred to nitrocellulose membranes (GE Healthcare). The blots were blocked in 5% milk and incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare) were performed according to our published procedure (Ninagawa et al. ...
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bioRxiv - Genomics 2023Quote: ... 167 mM NaCl) and pre-cleared with protein-A-Sepharose beads (GE Healthcare 17-5280-02) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate containing the protein of interest was loaded onto a 5 mL HisTrap column (GE Healthcare) equilibrated in 200 mM potassium phosphate ...
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bioRxiv - Immunology 2023Quote: ... Proteins were transferred onto an Amersham™ Protran™ 0.45 μm nitrocellulose membrane (GE Healthcare, UK). Membranes were blocked with BlueBlock PF (Serva Electrophoresis GmbH ...
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bioRxiv - Microbiology 2023Quote: ... The protein was further purified on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) equilibrated in 10 mM HEPES/NaOH pH=8.0 ...
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bioRxiv - Immunology 2022Quote: ... Th gB-1 mRNA was purified by fast protein liquid chromatography (FPLC; Akta purifier; GE Healthcare)79 ...
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bioRxiv - Microbiology 2023Quote: ... The proteins separated by the SDS-PAGE were transferred to a nitrocellulose membrane (GE life Sciences). The membrane was then incubated for 1 h at room temperature or overnight at 4 °C in blocking buffer (5% non-fat milk powder diluted in TBST (20 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated using 12 %SDS-PAGE and blotted onto a polyvinylidene difluoride membrane (GE Healthcare). The membrane was blocked in 5% milk/PBS for 1 h followed by overnight incubation with mouse monoclonal anti-TFAM antibody (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... The resolubilized protein was bound to QFF anion exchange resin (GE Healthcare Life Sciences, Marlborough, MA) and eluted using a linear gradient of 0.2–0.6 M NaCl ...
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bioRxiv - Microbiology 2023Quote: ... protein was concentrated and further purified by size-exclusion chromatography in a Superdex-S200 (GE Healthcare) column equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were concentrated and further purified by size-exclusion chromatography in a Superdex-S200 (GE Healthcare) column using 50 mM Tris-HCl pH 8.0 ...
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Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulationbioRxiv - Biochemistry 2023Quote: ... Eluted protein was applied to a HiLoad 26/600 Superdex 200 pg column (GE life sciences) equilibrated with 150mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... The monomeric proteins were then purified by using the HisTrap nickel column (GE Life Sciences 17524701) for FPLC (ÄKTA start protein purification system ...
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bioRxiv - Cell Biology 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... 25µL of Protein L beads (Cat. no. Capto™ L resin, GE Healthcare Cat. no. 17547802), pre-equilibrated with binding buffer (20mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... Soluble protein was maintained at 4°C and purified using Ni-NTA affinity resin (GE Healthcare). A column wash was performed using pH 7.0 HisA containing 300 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2023Quote: ... Lysates were pre-cleared with 30µl of either protein-A-sepharose (GE Healthcare, 17-5280-01) or protein-G-sepharose beads (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... The protein solution was passed over two coupled HiTrap Desalting Columns (GE Life Sciences, Pittsburgh, PA) to remove DTT and buffer exchanged into 20 mM Tris (pH 7.4) ...
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bioRxiv - Biophysics 2023Quote: ... The protein was purified by affinity chromatography using the Ni2+-Sepharose fast flow resin (GE Healthcare), pre-equilibrated with 10 column volumes of buffer A containing 1 mM DTT and gravity loaded with the supernatant from the preceding ultra-centrifugation step ...
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bioRxiv - Biochemistry 2023Quote: ... the concentrated protein was applied onto a Superose 6 Increase 10/300 GL column (GE healthcare) equilibrated with SEC buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... The eluted proteins were further purified via gel filtration (Superdex 200, GE Healthcare Chicago, IL, USA). In all cases ...
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bioRxiv - Neuroscience 2023Quote: ... The eluted proteins were further purified via gel filtration (Superdex 200, GE Healthcare Chicago, IL, USA). In all cases ...
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bioRxiv - Neuroscience 2023Quote: ... and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS, GE Healthcare, Chicago, IL) to remove nucleotide contaminants ...
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bioRxiv - Molecular Biology 2023Quote: ... The dialyzed protein was concentrated and injected into a HiLoad S75 16/600 column (GE Healthcare) pre-equilibrated with GST SEC buffer ...
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bioRxiv - Systems Biology 2023Quote: ... proteins in 2% SDS were bound to paramagnetic beads (SeraMag Speed beads, GE Healthcare,#45152105050250,#651521050502) by addition of ethanol to a final concentration of 50% ...