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Citations for GE Life Sciences :
2451 - 2500 of 2675 citations for 8 CHLORO 2 METHYL IMIDAZO 1 2 A PYRIDINE 3 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with secondary antibodies conjugated to different fluorophores (1:750 dilution) and were imaged in the laser-based InCell high-content imaging system (IN Cell Analyzer 6000, GE Healthcare). The nuclear levels of S9.6 or the number of γ-H2AX foci was quantified using the INCarta software that is attached to the imaging system ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase conjugated antibodies were added at a concentration of 1:7000 in 5% milk in TBST (HRP anti-mouse NA931V; HRP antirabbit NA934V, both GE Healthcare) and detected using chemiluminescence Clarity or Clarity Max ECL western blotting substrate (Biorad 1705061 or 1705062).
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bioRxiv - Cell Biology 2024Quote: ... Pek1 and Atf1 were generated by PCR of the corresponding gene fragments from yeast genomic DNA or cDNA and cloned in frame into expression vectors pGEX-4T-1 (GE Healthcare) or pMAL-c2x (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... The resulting lysate underwent clarification through centrifugation (125,000g for 30 minutes at 277 K) using a Ti-45 rotor (Beckmann) and was subsequently applied to a 1 ml HisTrap HP column (GE Healthcare). The column underwent an initial wash with five column volumes of lysis buffer containing 40 mM imidazole at pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant clarified from the cell lysate after overnight induction by 0.5mM isopropylthio-β-D-1-galactopyranoside (IPTG) at 20 °C was applied to pre-equilibrated 5Lml HisTrap column (GE Healthcare). After thorough wash with binding buffer (20LmM Tris–HCl pHL7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were obtained 24 hours after treatment and GST was purified on a 1 mL GSTrap HP column (GE Healthcare) with an AKTA purifier 10 FPLC system ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies for Western blotting were HRP-linked sheep anti-mouse IgG and donkey anti-rabbit (both GE Healthcare; 1:5000) and HRP-linked mouse anti-rabbit ...
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bioRxiv - Genomics 2024Quote: ... WSC3-e cells (1.6 µg protein µL-1) was loaded onto a Superdex 200 10/300 column (GE Healthcare, Chicago, USA), which was equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.84) ...
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bioRxiv - Biochemistry 2024Quote: ... Origin substrates were amplified using standard PCR protocols and were purified by anion exchange chromatography using a 1 ml RESOURCE Q column (GE healthcare). Peak fractions were ethanol precipitated and resuspended in TE buffer.
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bioRxiv - Biophysics 2024Quote: ... The filtered supernatant was then incubated at 4 ºC with gentle shaking at 5 rpm for 1 hour in the presence of 5 mL of HisPur Cobalt Resin (GE Healthcare). After multiple rounds of resin cleaning using Extraction Buffer supplemented with 5 mM imidazole ...
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bioRxiv - Neuroscience 2023Quote: ... Reaction products were electrophoretically resolved on 6% denaturing sequencing gel for 1 hour at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare). Nuclease activity quantification compared the densitometric intensity of cleaved versus un-cleaved DNA (ImageQuant ...
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bioRxiv - Cell Biology 2023Quote: ... was applied to a previously equilibrated HiTrap Chelating HP 5 ml column charged with 50 mM CoCl2 and coupled to a GSTrap HP 1 ml column (both GE Healthcare). Buffer A was used for equilibration ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was clarified by centrifugation at 30,000 x g (Sorval SLA 1500) at 4 °C for 30 min and loaded onto a 1 ml HisTrap HP column (GE Healthcare) on an ÄKTA purifier 10 system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... 70 μL of each sample at 1 mg/mL was fractionated in GF150 buffer using a Superdex 200 5/150 analytical gel filtration column (GE Healthcare) run at 0.1 mL/min on an Agilent 1200 series LC system collecting UV at 280 nm before then passing through the light scattering and refractive index detectors in a standard SEC MALS format ...
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bioRxiv - Biochemistry 2023Quote: ... of GcapOCP2 or CrinOCP2 holoform samples (1 mg/ml in 100 μl) was carried out on a Superdex 200 Increase 10/300 column (GE Healthcare) connected to a tandem of consecutive detectors Prostar 335 (Varian ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The digested DNA fragments were separated by electrophoresis using 1% agarose gels and transferred overnight to Hybond-N+ membranes (GE Healthcare) in 20 × SSC ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Bioengineering 2023Quote: ... A Biosep 10L acoustic filter (Applikon Inc., Foster City, CA) related to the bioreactor thanks to two peristaltic pumps (P-1, GE Healthcare), one for medium recirculation and one for sample withdrawal (Henry et al. ...
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bioRxiv - Biophysics 2023Quote: ... The fixed cells were rinsed three times in warm PBS and incubated for 30 min with a blocking solution containing 1% BSA (GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was stained with DAPI (1 µg/mL) for 5 minutes at room temperature and cells imaged with a DeltaVision Core system (Applied Precision).
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were washed three times in PBST for 15 min and were then incubated with HRP-conjugated secondary antibodies (1:2,500 dilution; GE Healthcare, NA9340V) for 45 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Insoluble fractions were removed by centrifugation at 20000 g during 30 min at 4°C and the supernatant was loaded on a 1 mL HisTrapTM HP column (GE Healthcare) connected to a NGC chromatography system (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μl of each sample was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA), and the dry membrane was incubated in NRP solution (0.01 g/ml KMnO4 in ddH2O ...
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bioRxiv - Biophysics 2023Quote: ... The solution was concentrated to ∼1 ml and went through gel filtration on Superdex 75 10/300 increase column (GE Healthcare) equilibrated with a buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... Final purification and concentration of the untagged DAP5 was performed using a 1 ml HiTrap heparin HP column from GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2023Quote: ... As done in our previous experiments (Cattaneo et al., 2022) stimuli aliquots were spread on grade 1 – 20 mm circles filter paper (GE Healthcare Life Science ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3x for 10 min with TBST and subsequently incubated for 1 hr at room temperature with appropriate horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) diluted (1:10,000 ...
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bioRxiv - Plant Biology 2023Quote: ... Vectors suitable for expression and purification of His6-and/or glutathione S-transferase (GST)-tagged recombinant proteins in Escherichia coli were either cloned by Twist Biosciences into the pET28a+ backbone (EMD Biosciences) or cloned in-house into the pGex6p.1 backbone (GE Healthcare). When needed ...
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bioRxiv - Molecular Biology 2023Quote: ... the TEV-cleaved proteins were purified in two steps: 1) IEX on 5 mL HiTrap Q HP (GE Healthcare Life Sciences) using as buffer A ...
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bioRxiv - Microbiology 2023Quote: ... with a 10 kDa cut-off and loaded onto a Superdex 200 pg HiLoad 16/600 (GE Healthcare, 1 mL/min). Sample purity was assessed by 12% SDS-PAGE analysis and sample concentration was determined by spectrophotometry using a theoretical molar extinction coefficient at 280 nm of 20 ...
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bioRxiv - Cell Biology 2023Quote: ... or phosphate buffered saline containing 1% human serum albumin (Grifols Biologicals, Los Angeles, CA, USA) using either a PD10 desalting column (GE Healthcare) or a Vivaflow 50 cassette (Sartorius ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used in immunoblotting: HRP-conjugated donkey anti-rabbit IgG (1:2000) (GE Healthcare, catalog number AB_772206), HRP-conjugated goat anti-mouse IgG (1:5000 ...
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bioRxiv - Systems Biology 2023Quote: ... Isolated cells were washed once in PBS (2mM EDTA, 1% FBS) and lymphocytes were enriched on a 40-67% Percoll gradient (GE Healthcare).
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bioRxiv - Immunology 2024Quote: Tested antibody (5 μg/mL for NTD and 1 μg/mL for spike) was captured on protein A sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified SARS-CoV-2 mutant spike or NTDs ...
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bioRxiv - Physiology 2024Quote: ... Membranes were washed twice with TBS containing 0.1% Tween-20 and once with PBS containing 0.1% Tween-20 and then incubated with goat anti-mouse or goat anti-rabbit horseradish peroxidase (HRP)-conjugated IgG (GE Healthcare) diluted 1:10000 or 1:5000 ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used for immunoblotting: HRP-conjugated donkey anti-rabbit IgG (1:2000) (GE Healthcare, catalog number NA934) and HRP-conjugated donkey anti-mouse IgG (1:5000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The Triton X-100 was then removed by either (i) binding to a HisTrap 1 mL column (GE Healthcare, Chicago, USA) or HIS-Select Nickel Affinity Gel in a gravity-flow column (Merck ...
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bioRxiv - Bioengineering 2024Quote: The RSVF RSVF-multi-1 immunogen and motavizumab Fab were purified by size exclusion chromatography using a Superdex200 26 600 (GE Healthcare) equilibrated in 10 mM HEPES pH 7.5 and 150 mM NaCl and concentrated to ∼20 mg/ml (Amicon Ultra-15 ...
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bioRxiv - Biophysics 2024Quote: ... TEV-cleaved protein was then diluted 1:500 in KBT and then loaded onto a HiTrap Q HP column (GE Healthcare) for ion exchange chromatography (IEC ...
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bioRxiv - Molecular Biology 2024Quote: ... This lysate was applied onto a 1 mL HisCap Smart 6FF column (Smart-Life Sciences) using an Akta Purifier 10 chromatograph (GE Healthcare). Subsequent washing steps were carried out with mix of buffer A and buffer B (buffer A + 500 mM imidazole) ...
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bioRxiv - Neuroscience 2024Quote: ... The protein bands were detected by incubating the membrane with primary antibodies (see below) overnight at +4°C and horse radish peroxidase-conjugated secondary antibodies (1:5000, NA934 or NA931, GE Healthcare) for 1 h at RT ...
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was discarded and the pellet was resuspended in 1 mL of Percoll® (GE Healthcare Bio-Science AB, Sweden). After strong agitation followed by centrifugation ...
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bioRxiv - Immunology 2024Quote: ... Complexes were generated by incubating Fab and antigen overnight at a 50:1 ratio and purified through size exclusion chromatography with a Superdex 200 increase 10/300 column (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... A peroxidase-conjugated secondary antibody was used at a dilution of 1:5000 and immunoreactive bands were visualized with ECL Western Blotting Detection Reagents (GE Healthcare, UK).
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bioRxiv - Biophysics 2020Quote: ... which contains DnaK protein was purified first with ion exchange chromatography using 0 to 1 M NaCl in a HiTrap column (HiTrap, GE Healthcare, Germany). Primary fraction of DnaK was eluted in 150 mM NaCl which was further purified using size exclusion chromatography using a superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... for 1 h at 37 °C and the RNA was desalted twice by Illustra Microspin G-25 columns (GE Healthcare Life Sciences). Method for EMSA was modified from a previously published protocol (30) ...