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Citations for GE Life Sciences :
2351 - 2400 of 2675 citations for 8 CHLORO 2 METHYL IMIDAZO 1 2 A PYRIDINE 3 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... The pellet was dissolved in 10 mL of buffer B and loaded onto a 1 mL HiTrap Heparin HP sepharose column (GE Healthcare) equilibrated with buffer B supplemented with 0.1 M NaCl ...
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bioRxiv - Biophysics 2020Quote: ... Cells were pelleted at 3500 g and the media containing secreted zRETECD was pooled and 1 ml of IgG sepharose resin (GE Healthcare) was added to 1 l of media and incubated at 4 °C for 18 hrs ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were then incubated for 1 h with a secondary antibody (goat anti-rabbit horseradish peroxidase-linked IgG [NA934V GE Healthcare] or sheep anti-mouse horseradish peroxidase-linked IgG [NA931V GE Healthcare] ...
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bioRxiv - Immunology 2021Quote: The scCl5 was purified using a two-steps protocol using first immobilized metal affinity chromatography (IMAC) to enrich for His tagged protein (1 ml HisTrap, GE Healthcare) followed by anion exchange (1 ml HiTrapQ ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from 1 ml overnight bacterial culture using the Illustra bacteria genomicPrep Mini Spin Kit (GE Healthcare, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The mix was allowed to incubate for 1 hour over ice and loaded to a Superdex 200 Increase 10/300 GL size exclusion column (GE healthcare) pre-equilibrated in buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: Fragment screening of MsbA embedded in nanodiscs was performed with the Discovery-ZEN-LIBRARY 1 (Zenobia) in a Biacore 8K instrument (GE Healthcare) using an NTA Series S sensor chip (Cytiva ...
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bioRxiv - Biophysics 2020Quote: ... including an N term extension containing His9-HRV 3C protease site was cloned into the BamHI EcoRI sites in the MCS of pGEX-6P-1 vector (GE Healthcare). Site-directed mutagenesis was performed on the His9-SARS-CoV-2 Nucleocapsid pGEX vector to create the N protein constructs (SI Table XX and sequences were verified using Sanger sequencing ...
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bioRxiv - Physiology 2022Quote: ... membranes were washed three times with 1X TBST and incubated with secondary antibodies in 5% milk in TBST for one hour at room temperature with the following concentration of secondary antibodies: donkey anti-rabbit IgG (1:5000, NA934, GE Healthcare) and goat anti-mouse IgG (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: GST or GST-tagged β’-COP (residues 1-604) or Glo3 constructs (GAP domain, BoCCS region, GRM region) were immobilized on glutathione sepharose resin (GE Healthcare) for one hour on ice ...
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bioRxiv - Microbiology 2022Quote: ... cleavage was achieved after elution of GbpA from the column by adding 1 unit of PreScission protease enzyme and incubation overnight at 4°C per the instructions of the manufacturer (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: IVTT reaction mixtures (100 µl) were incubated at 4 °C for 1 h with a 50% slurry of PBS/Glutathione-Sepharose 4B (GE Healthcare) with gentle mixing ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to less than 1 mL and was injected onto a Superdex 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was separated by further centrifugation at 13 000 x g for 1 h at 4 °C and the supernatant was loaded onto a 5 mL HisTrap FF NiNTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... The remainder of the lysates were incubated with polyclonal rabbit anti-GFP (EU2; Eusera) for 1 hour followed by Protein A-Sepharose beads (GE Healthcare) for another hour at 4° C ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting supernatant (∼1 ml) was incubated with 20 µl of dimethyl pimelimidate (DMP) cross - linked IgG Sepharose 6 Fast Flow (GE healthcare) resin for 2 h with gentle rotation at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant protein in the soluble cell fraction was purified by immobilized metal affinity chromatography (IMAC) using a HiTrap Chelating HP 1-ml column (GE Healthcare) with an ÄKTA purifier FPLC system (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... Human CFP1 and truncation constructs were subcloned into the pGEX-6P-1 vector encoding an N-terminal glutathione S-transferase (GST) tag (GE Healthcare) prior to expression in Origami cells ...
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bioRxiv - Biophysics 2020Quote: ... Fixed cells were rinsed three times in warm PBS and incubated 30 min with a blocking solution containing 1% BSA (Bovine Serum Albumine, GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Biochemistry 2019Quote: ... Afterwards the immobilized papain resin was removed and the digested fraction was mixed with Protein A Sepharose (0.2 ml resin for 1 mg digested IgG, GE Healthcare, #17078001) for 1 hour at RT ...
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bioRxiv - Plant Biology 2019Quote: ... Slides were rinsed as before and incubated with a 1/100 dilution (in PBS/BSA) of biotinylated/streptavidin/horseradish peroxidase complex (GE Healthcare) before a final PBS/BSA rinse ...
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bioRxiv - Molecular Biology 2019Quote: ... was added to the protein solution to a final concentration of 1.3 μM and incubated for 15 minutes at room temperature in order to cleave off His10-SUMO tag and the protein solution was applied on HisTrap 1 HP (GE Healthcare) loaded with Zn2+ and pre-equilibrated with storage buffer ...
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bioRxiv - Biophysics 2020Quote: ... Bound proteins were eluted using a linear gradient (10 column volumes) from 0 to 1 M NaCl in buffer A using an Äkta Purifier (GE Healthcare). Elution fractions (1.6 ml ...
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bioRxiv - Immunology 2019Quote: ... ERV-K envelope protein was cloned out of the sequencing vector and ligated into pGEX-6p-1 N’ GST-tag expression vector (GE Healthcare) between EcoRI and NotI sites using the following primers:
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bioRxiv - Biochemistry 2019Quote: ... 0.1 ml of this solution was subjected to SEC at 293 K on a Superdex 200 10/300 column (GE Healthcare) preequilibrated in 10 mM MES pH 6.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were centrifuged for 45 minutes at 100,000 x g and the supernatant was incubated for 1 hour with 5 mL Ni-NTA resin containing HisTrap FF column (GE Healthcare), which binds the gelsolin-actin complex through an N-terminal polyhistidine affinity tag on the gelsolin fragment ...
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Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1bioRxiv - Biochemistry 2019Quote: ... concentrated using centrifugal filters to bring down the volume to ∼1 mL and injected onto a Superdex 200 16/60 size exclusion column (GE Healthcare) previously equilibrated in HEPES 50 mM ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... 600 µL of a 1:10 dilution of serum was applied onto the protein G column (Ab Spin trap; GE Healthcare) and washed ...
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bioRxiv - Plant Biology 2021Quote: ... Fractions containing LPR1 (detected by immune blot analysis) were directly applied to cation exchange carboxymethyl-sepharose column (HiTrap CM FF, 1-ml, GE Healthcare) equilibrated with buffer B (20 mM Na2HPO4-NaH2PO4 ...
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bioRxiv - Plant Biology 2020Quote: ... an additional anion exchange (AIEX) step was performed prior to SEC by passing the protein over a 1 mL AIEX column (GE Healthcare). SnTox3 protein ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were then incubated with HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (1:2500, GE Healthcare Life Sciences) diluted in 2% non-fat milk prepared in PBS for 1 hour at RT followed by 3 washes with TBS containing 0.1% Tween-20 for 5 minutes each at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were separated on 12.5% (v/v) acrylamide gels of 25 cm x 21 cm x 1 mm using an Ettan DALTsix electrophoresis unit (GE Healthcare). The SDS-PAGE was first run at a current of 2 W/gel for 1 h and then at a constant current of 15 W/gel for 5 h ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h at 4 °C and purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM sodium phosphate pH7.4 ...
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bioRxiv - Biochemistry 2020Quote: SEC-MALS analysis was performed by injecting 100 μl of purified protein at 1 mg/mL onto a Superdex 200 10/300 Increase GL column (GE Healthcare), pre-equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Final gels were exposed to a storage phosphor screen for 1 hour and scanned using a Typhoon Imager 9410 (GE Healthcare). Band intensities were determined using the Fiji ImageJ software49 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Samples were topped up to 1 ml with Buffer A and loaded onto a HiPrep 16/60 Sephacryl S-500 HR column (GE Healthcare). Buffer A was run at 1 ml/min and the flow-through was collected in 5 ml fractions ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared by centrifugation for 1 hour at 60000 x g and then loaded on a 5 mL Histrap FF column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins (500 μl) at a concentration of 1 mg/ml were loaded on a Superdex 75 10/300 GL column (GE Healthcare) and eluted with HEPES buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... using pE-SUMO as an expression plasmid (86) and was isolated using a 1-mL HisTrap column and Äkta start (GE Healthcare). Before using the fusion protein for far western blotting ...
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bioRxiv - Microbiology 2020Quote: ... After incubating with horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG as a secondary antibody at 1:2000 dilution (GE Healthcare.), protein bands were visualized by chemiluminescence and the image was captured by ImageQuant LAS 4000 mini (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... 400 μl of purified protein at 1.4 mg·ml−1 was fractionated on a Superdex 200 10/300 column equilibrated in GF buffer using an AKTA purifier (GE Healthcare) at a flow rate of 0.5 ml·min−1 ...
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bioRxiv - Biochemistry 2021Quote: ... 1mM CaCl2 (pH 8.0)) at a flow rate of 1 mL/min on an ÄKTA-Pure instrument (GE Healthcare, Piscataway, NJ). The purified proteins were analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions were analysed using SDS-PAGE and peak fractions were collected and further purified using a 1 ml MonoQ column (GE Healthcare). Sic1 was eluted with 20 column volumes (CV ...
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bioRxiv - Immunology 2020Quote: ... boiled and separated by gel electrophoresis in Tris-glycine SDS (TGS) buffer (20 mM Tris, 192 mM glycine, 1 % (w/v) SDS) and transferred to a nitrocellulose membrane (GE Healthcare) in Tris glycine (TG ...
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Microbiology 2019Quote: ... lysed with 100 μg lysozyme and sonication and the filtered cell lysate loaded onto a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Then the peak fractions were desalted and further purified by ion-exchange chromatography using 1-ml Resource S column (GE Healthcare) in buffer E (20 mM mM HEPES ...
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bioRxiv - Zoology 2019Quote: ... DNA quality was assessed by running a subsample on a 1% agarose gel and the quantity of DNA was measured using a NanoVue (GE Healthcare). DNA libraries were constructed by Macrogen using a TruSeq DNA PCR-free library kit (350bp insert size) ...