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Citations for GE Life Sciences :
201 - 250 of 673 citations for SARS Coronavirus Nucleoprotein NP His tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The crude extract was applied onto a nickel chelation column (His GraviTrap, GE Healthcare), and TasA28-261-His was eluted according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Subsequently hCD14-his was purified on a 5 mL HisTrap Excel column (GE Healthcare) by employing ÄKTA Pure system (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... and His(6)GFP –AnkBD proteins were purified using Glutathione Sepharose 4B (GE Healthcare) or Talon® (Clontech ...
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bioRxiv - Biochemistry 2022Quote: ... the His-tagged DnaK protein was purified using a HisTrap HP column (GE Healthcare) with 2×PBS buffer followed by further purification using a HiTrap Q column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was desalted using a His Trap Desalting columns from GE Healthcare according to the prescribed protocol and was stored at −80°C with 40 % glycerol.
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified over 1-2 ml His-Trap FF columns (GE Healthcare) with elution buffer containing 250 mM Imidazole ...
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bioRxiv - Plant Biology 2022Quote: ... The His-MDIS1CD protein is loaded onto a Ni-NTA agarose column (GE Healthcare) and eluted using elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... and applied to a Hi Trap SP HP column (GE Healthcare #17-1151-01) equilibrated with dCas9-Cys dialysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... and applied to a Hi Trap SP HP column (GE Healthcare #17-1151-01) equilibrated with dCas9 dialysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were applied to Nickel-Nitrilotriacetic (Ni-NTA) His-Trap FF column (GE Healthcare) and 6xHis-tagged-mCherry-p62 was eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... GgUox was purified using a 5 mL His-trap Ni-NTA column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... loaded onto a Hi-Trap heparin affinity column (2×5 ml, GE Life Sciences), and eluted with a salt gradient (200–800 mM NaCl) ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was passed through a His Prep FF 16/10 column (GE Healthcare) and washed with 5 column volumes of buffer ...
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bioRxiv - Biophysics 2023Quote: ... Subsequent removal of DTT was achieved with a Hi-Trap desalting column (GE Healthcare) equilibrated in buffer A without β-mercaptoethanol ...
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bioRxiv - Biochemistry 2024Quote: ... ZapD and His-Smt3 were separated by chromatography in 5-ml HisTrap (GE Healthcare), then the protein was eluted in a 5-ml HiTrap Desalting column (Healtcare ...
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bioRxiv - Immunology 2022Quote: ... Tags were removed by Enterokinase treatment and separated by HiTrap SP HP (GE Healthcare).
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bioRxiv - Immunology 2019Quote: ... Chimeras bearing tandem affinity purification tags were captured using streptavidin-sepharose beads (GE Healthcare). SDS-PAGE gels were transferred to either Immobilon-P (EMD Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... The GST affinity tag was then cleaved using the PreScission Protease (GE Life Sciences) for four hours in cleavage buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare) in 150 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... The tag-free proteins were further purified by anion exchange chromatography (MonoQ; GE Healthcare). Peak fractions were collected ...
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bioRxiv - Immunology 2021Quote: ... and then isolated by Strep-tag affinity chromatography on StrepTrap HP columns (GE Healthcare), followed by size-exclusion chromatography on TSKgel G4000SWXL (TOSOH ...
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bioRxiv - Biochemistry 2023Quote: ... The GST tag was removed using PreScission protease (GE Healthcare Life Sciences (now Cytiva)) ...
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bioRxiv - Biochemistry 2023Quote: ... The GST-tag was removed by an on-column cleavage using thrombin (GE Healthcare) at a final concentration of 1 unit/mL ...
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bioRxiv - Biophysics 2020Quote: ... 0.05% NP-40) and assembled complexes were affinity purified from the cushions using lgG Sepharose 6 Fast Flow (GE Healthcare) to capture the Protein A tag ...
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bioRxiv - Bioengineering 2022Quote: ... The assembled 6RBD-np was purified by size exclusion chromatography using Superdex 200 Increase 10/300 GL column (GE Healthcare) in DPBS ...
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bioRxiv - Immunology 2022Quote: The binding kinetics of nanobodies to SARS-CoV-2 RBD were analyzed using SPR (Biacore 8K; GE Healthcare). The nanobodies were captured by the ProA sensor chip and serial dilutions of SARS-CoV-2 RBD were then flowed through at a rate of 30 μL/min in PBS buffer (with 0.05% Tween20 ...
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bioRxiv - Microbiology 2020Quote: ... was cloned into mouse IgG1 or human IgG1 Fc backbone in pKN293E expression vectors and transiently transfected into HEK293 cells followed by media collection and purification using MabSelect SuRe antibody purification resin (Cat: 29-0491-04, GE Healthcare). SEC-HPLC and SDS-PAGE were used to check the size and purity of these recombinant proteins.
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bioRxiv - Biochemistry 2020Quote: The recombinant catalytic domains of MMP-2 and -9 were expressed in HEK293 cells stably transfected with respective constructs and purified from serum-free culture medium using Gelatin Sepharose 4B (GE Healthcare) as described in [35 ...
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bioRxiv - Immunology 2020Quote: IgGs were expressed by transient transfection in HEK293-6E cells and purified from cell supernatants using protein A or G (GE Healthcare) as previously described (39 ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were purified by Ni-affinity chromatography on 5 mL His-Trap columns (GE Healthcare) as follows ...
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bioRxiv - Molecular Biology 2021Quote: 1ug of soluble His-Rap1 was pre-cleared on Glutathione sepharose 4B beads (GE Healthcare) for 1h at 4C ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant protein was purified under denaturing conditions over a His-SpinTrap column (GE Healthcare), desalted using a PD SpinTrap G-25 column (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tagged UBC13 and its variants were bound to HisTrap HP columns (GE Healthcare) and were eluted with 100mM-500mM Imidazole gradient ...
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bioRxiv - Biochemistry 2020Quote: ... hydrophobic interaction chromatography using a Hi-Trap Butyl-Sepharose column (GE Healthcare, Cat. No. 28411001) with a flow rate of 1 ml/min and a gradient from 0.9 M (NH4)2SO4 in PBS to PBS over 5 CV was performed ...
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bioRxiv - Biochemistry 2020Quote: ... the soluble fraction was loaded onto a His Trap HP 5 mL column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded onto a 1mL His-Trap FF column (GE Healthcare Life Sciences) and ZapB was eluted with an imidazole gradient (0 to 0.5M) ...
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bioRxiv - Microbiology 2020Quote: ... Concentrated samples were loaded onto Hi Load 16/600 Superdex 75 pg column (GE Healthcare) connected to the NGC Scout Chromatography system and running buffer composed of 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was applied to a Hi-Load Superdex 200 16/60 column (GE Healthcare) in a buffer A ...
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bioRxiv - Microbiology 2020Quote: ... And further purified by gel filtration on a Hi Load Superdex 75 column (GE Healthcare). Peak fractions were concentrated to 10 mg/ml in 20 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... RBD protein was then captured using His-Trap HP column (GE Healthcare, Wauwatosa, WI, USA) or HiTrap TALON crude column (Cytiva ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged ZO-1-PDZ2 were purified using a HiTrap Chelating HP (GE Healthcare), where a renaturation gradient by exchange of denaturation buffer to a gel filtration buffer (Tris 50 mM (pH 7.5) ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant (crude extract) loaded on a 1-ml His-Trap column (GE Healthcare) previously equilibrated with Buffer H ...
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bioRxiv - Bioengineering 2021Quote: ... Immobilized metal affinity chromatography (IMAC) using His-Trap FF Ni+2-NTA column (GE Healthcare) attached to BioRad™ NGC system ...
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bioRxiv - Synthetic Biology 2022Quote: ... The soluble fraction was then loaded onto a His-Trap column (GE Healthcare Life Sciences) and eluted with buffer B (100nM NaCl and 500 mM imidazole in 50 mM Tris at pH 8.0) ...
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bioRxiv - Immunology 2019Quote: ... The supernatant was then applied to a nickel-affinity chromatography (His Trap excel, GE Healthcare) equilibrated with buffer B (20mM Tris pH 7.2 ...
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bioRxiv - Immunology 2020Quote: ... the His-tagged proteins were purified using nickel-affinity chromatography (HiTrap chelating, HP; GE Healthcare) with an imidazole gradient (10-250 mM ...
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bioRxiv - Biochemistry 2021Quote: ... was purified by IMAC on a Ni2+-His-Trap column (GE Healthcare, Chicago, IL, USA) in buffer 0,1M K-phosphate pH 7,5 ...
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bioRxiv - Microbiology 2021Quote: ... 10 mM Imidazole (pH 8.0)) using a Hi-Prep Desalting 26/10 column (GE Healthcare). To remove the HIS-myc affinity tag ...
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bioRxiv - Microbiology 2020Quote: ... GST fusion protein and His fusion protein were purified with glutathione-Sepharose 4B (GE Healthcare) and Ni-NTA Agarose (QIAGEN ...