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Citations for GE Life Sciences :
201 - 250 of 5689 citations for Human Immunodeficiency Virus GP41 Protein HIV 1 Group O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Sample protein masses were assessed with Amersham high molecular weight protein marker mix (GE Healthcare) with proteins porcine thyroglobulin (Rs=17.0nm) ...
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bioRxiv - Microbiology 2020Quote: ... GST fusion protein and His fusion protein were purified with glutathione-Sepharose 4B (GE Healthcare) and Ni-NTA Agarose (QIAGEN ...
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bioRxiv - Bioengineering 2023Quote: For all protein variants a protein A chromatography (MabSelect SuRe resin, GE Healthcare, Uppsala, Sweden) was used as capture step ...
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bioRxiv - Cell Biology 2023Quote: ... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Immunology 2023Quote: ... the filtered supernatant ran through a protein A and protein G affinity column (GE Healthcare) and eluted with 0.2 M citric acid at pH 3.0 and neutralized in 2 M Tris-base ...
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bioRxiv - Cell Biology 2023Quote: ... The complex was pulled down using Protein A or Protein G-sepharose beads (GE Healthcare) pre-equilibrated in lysis buffer for 2 h ...
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bioRxiv - Cell Biology 2020Quote: Cells lysates were prepared using 1 % triton lysis buffer and precleared with prewashed Protein G Sepharose (PGS) beads (GE Healthcare) for 30 min on a shaker at 4 °C ...
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bioRxiv - Physiology 2022Quote: ... Supernatants were collected after centrifugation at 13,200 rpm for 15 min and precleaned for 1 h with 50% protein G-Sepharose (GE Healthcare), followed by incubation with an indicated antibody at 4 °C Upon addition of 100 μl of 50% protein G-Sepharose ...
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bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The protein purification was done through affinity chromatography on a Ni-column (HiTrap chelating HP column 1 ml, GE HealthCare). The column was loaded with a filtered solution of 100 mM nickel chloride ...
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bioRxiv - Immunology 2020Quote: ... IgGs were purified from ∼1-3 ml of serum using equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted off the resin with 0.1 M glycine pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2019Quote: ... the dialyzed protein was filtered and loaded on a hydrophobic HiTrap Butyl HP 1 ml column (GE healthcare life sciences), pre-equilibrated with 5 column volumes of buffer AButyl (500 mM ammonium sulfate ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation for 1 hour at room temperature proteins were purified using PD MiniTrap™ G-25 columns (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... cells were lysed using M110-L microfluidizer (Microfluidics) and the protein was isolated using 1-mL HisTrap column (GE Healthcare). Protein was concentrated with Amicon Ultracel-10K (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Alba proteins were run on an ion-exchange column (HiTrap SP column, 1 ml, GE Healthcare UK Ltd. Buckinghamshire, England) following the manufacturer’s protocol and as described previously (Maruyama et al. ...
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bioRxiv - Immunology 2020Quote: ... for 1 h at 4 °C in an overhead tumbler before retrieving immune complexes with protein G Sepharose (GE Healthcare). Beads were washed and complexes were treated with Endoglycosidase H (New England Biolabs ...
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bioRxiv - Biophysics 2020Quote: The protein extract was loaded onto a 1 mL HiTRAP Heparin column HP (GE Healthcare Life Sciences, 17-0406-01) equilibrated with Buffer A (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... Approximately 1 mg of total cytosolic proteins was injected onto a Superdex® 200 10/300 GL column (GE Healthcare). The SEC elution was performed with the mobile phase [50 mM Hepes/KOH ...
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bioRxiv - Biochemistry 2021Quote: ... anti-EGFR antibody (#MI-12-1, MBL), anti-GFP antibody (#M-048-3, MBL) and Protein A-Sepharose (GE Healthcare) were used for immunoprecipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in the lysates was determined by Bradford assay against BSA and 1-3mg protein-lysate equivalent was loaded onto 10μL of bead slurry (Dynabeads, GE healthcare) prewashed twice with lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... the protein was purified using HisTrap HP histidine-tagged protein columns (GE healthcare, Chicago, IL, USA) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with protein A or protein G Sepharose beads (GE Healthcare, Milwaukee, WI, USA) for 3 h with gentle rocking in the cold room ...
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bioRxiv - Immunology 2020Quote: ... The IgG was then purified using protein G affinity chromatography (HiTrap Protein G HP, GE Healthcare) and size-exclusion chromatography (Superdex 200 ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were exchanged into protein storage buffer on an NAP-5 column (GE Healthcare, 17085301), concentrated with a 50 kDa MWCO Amicon Ultra-0.5 device (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... Large scale protein purification was done using ÄKTA prime Fast Liquid Protein Chromatography System (GE Healthcare). DE droplets were analyzed on a BD LSR Fortessa SORP (Special Order Research Product ...
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bioRxiv - Immunology 2021Quote: ... and the protein within the supernatant was purified with a Protein A-Sepharose column (GE Healthcare) according to the manufacturer’s instruction for primary purification ...
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bioRxiv - Biochemistry 2022Quote: Antibodies against CFTR were pre-incubated with protein-A or protein-G Sepharose beads (GE Healthcare) for 15 minutes at 4°C before adding protease-treated or non-treated lysates ...
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bioRxiv - Microbiology 2022Quote: ... and isolated from supernatants by affinity chromatography using Protein A or Protein G columns (GE Healthcare). MAbs were eluted with 0.1 M glycine at low pH and dialyzed into PBS.
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bioRxiv - Microbiology 2024Quote: ... The mutated proteins were purified from the media using a Protein-A affinity column (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: Human peripheral blood mononuclear cells were isolated by Ficoll (GE Healthcare) density gradient centrifugation and then stained with PE anti-CD3 (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... human or rabbit IgG (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) were used and HRP activity detected using ECL plus (GE Healthcare Bio-Sciences Corp. ...
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bioRxiv - Cell Biology 2020Quote: ... Protein G sepharose beads (GE Healthcare) were washed three times in lysis buffer to remove any residual ethanol from the storage buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and Protein G Sepharose (GE Healthcare) pre-blocked in 1% BSA-PBS were used to immunoprecipitate (IP ...
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bioRxiv - Cell Biology 2020Quote: ... Protein-G sepharose beads (GE Healthcare) were spun at 13000X g for 1 minute ...
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bioRxiv - Molecular Biology 2021Quote: ... A Protein-G column (GE Healthcare) was used for affinity purification of antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... Protein-G sepharose beads (GE Healthcare) were spun at 13000X g for 1 minute ...
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bioRxiv - Cell Biology 2021Quote: ... Protein G-agarose beads (GE Healthcare) were added to the lysate–antibody mix and left to rock at 4°C for 2 h ...
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bioRxiv - Plant Biology 2020Quote: ... RDR2 and protein standards (GE Healthcare) were subjected to FPLC using a Superdex 200 10/300 GL column at 4°C using 50 mM HEPES-KOH pH7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... protein G Sepharose beads (GE Healthcare) were blocked overnight with 0.5 mg BSA ...
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bioRxiv - Immunology 2022Quote: ... and Protein G Sepharose (GE Healthcare) was added to Expi293 supernatant for 2h at room temperature or overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Protein A sepharose beads (GE Healthcare) were washed twice and resuspended 1:1 with lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... and Protein G sepharose (GE Healthcare) respectively ...
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bioRxiv - Cell Biology 2023Quote: ... Protein G-Sepharose beads (GE Healthcare) were added to the supernatant and incubated at 4°C for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... Protein-G Sepharose beads (GE Healthcare) were blocked in 0.1% BSA for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Protein G–agarose beads (GE Healthcare) or magnetic beads were washed twice before immunoprecipitation.
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bioRxiv - Genetics 2023Quote: ... and protein G Sepharose (GE healthcare).
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bioRxiv - Immunology 2023Quote: ... with protein A column (GE Healthcare). MARV GP (Uganda strain ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins and SP3 beads (GE healthcare) were mixed in a 1:10 weight ratio in 80 % ethanol for 5 minutes at RT ...
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bioRxiv - Biochemistry 2023Quote: ... KTA Protein Purification Systems (GE Healthcare) and UNICORN Control Software version 5.11 (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: Protein A-Sepharose beads (GE Healthcare) were incubated with 2 µg of antibody (anti-V5 ...