Labshake search
Citations for GE Life Sciences :
1 - 50 of 5689 citations for Human Immunodeficiency Virus GP41 Protein HIV 1 Group O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: Serum samples from HIV-1-infected individuals were heat-inactivated at 56 °C for 40 min and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4 °C ...
-
bioRxiv - Biochemistry 2020Quote: ... MchDnaB1_HN and gp41-1_WCT were further purified using a Superdex® 75 10/300 column (GE Healthcare, Chicago, Illinois, USA) in buffer E (10 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
-
bioRxiv - Biochemistry 2020Quote: ... HyClone™ DMEM/F-12 (1:1; w/o phenol red) was purchased from GE Healthcare Life Sciences (Logan ...
-
bioRxiv - Microbiology 2019Quote: ... and O-glycosylated proteins isolated by affinity chromatography using Concavaline A columns (HiTrap Con A 4B, GE Healthcare) in AKTA FPLC (GE Healthcare) ...
-
bioRxiv - Immunology 2020Quote: ... Sera were then pooled for each group and IgGs were purified using protein G beads (GE Healthcare). The IgG concentration for each sample was obtained measuring the absorbance at 280 nm using a nanodrop (Thermo Scientific).
-
bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
-
bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
-
bioRxiv - Biochemistry 2022Quote: ... HIV-1 IN was further fractionated by size exclusion chromatography (SEC) with a Superdex 200 Increase (10/300) column (GE Healthcare) and stored at −80°C (Fig ...
-
bioRxiv - Neuroscience 2019Quote: ... P2 fractions were pre-cleared in NP-40 buffer for 4 hours and then incubated o/n at 4 °C with protein A-Sepharose beads (GE Healthcare, Milan, Italy) conjugated to 10 μg/ml of anti-Pin1 antibody (Santa Cruz Biotechnology ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein A and Protein G beads mix (1:1) (GE Healthcare) were washed with HEMG100 ...
-
bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
-
bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
-
bioRxiv - Cell Biology 2021Quote: ... (w/o L-glutamine and phenol red, GE Healthcare Bio-Sciences, Pittsburgh, PA). After removal of the medulla ...
-
bioRxiv - Immunology 2022Quote: ... a 1:1 solution of Protein A Sepharose (GE Healthcare) and Protein G Sepharose (GE Healthcare ...
-
bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
-
bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
-
bioRxiv - Microbiology 2021Quote: ... As mentioned above GFP positive HIV infected cells were enumerated by fluorescence microscopy using the high content CYTELL imaging platform (GE Healthcare). An average of 25,000 cells were analysed per condition by acquiring multiple fields of view ...
-
bioRxiv - Cell Biology 2019Quote: ... then lysed in RIPA buffer and incubated O/N with SpeedBeads Neutravidin magnetic beads (GE Healthcare). Pulled-down proteins and beads were then washed twice in RIPA ...
-
bioRxiv - Neuroscience 2021Quote: ... the Fc tag was cleaved by O/N incubation with GST-tagged 3C PreScission Protease (GE Healthcare) in cleavage buffer (150 mM NaCl ...
-
bioRxiv - Biophysics 2019Quote: ... DNA modified with a primary amine group was mixed with Cy3 NHS ester (GE Healthcare) at room temperature for 2 hours ...
-
bioRxiv - Microbiology 2023Quote: ... RNA (2 μg) of each group was spotted on a Hybond-N+ membrane (GE Healthcare), followed by UV cross-linking (120 mJ/cm2 ...
-
bioRxiv - Biochemistry 2020Quote: ... Virus was purified by heparin affinity chromatography on an Akta FPLC (HiTrap, GE Healthcare), ultra-centrifuged overnight and resuspended in sterile Ringer’s solution ...
-
bioRxiv - Neuroscience 2020Quote: ... in which they were precleared for 1 h using 30 μl (dry volume) of a 1:1 mixture of protein-A and protein-G-agarose beads (GE Healthcare, Chicago, IL, USA). Thereafter ...
-
bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
-
bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...
-
bioRxiv - Biophysics 2019Quote: Relative spot intensities by comparison group were estimated using ImageMaster 2D platinum 7.0 software (GE Healthcare). Those showing more than or equal to one fold difference between groups (frail vs nonfrail ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Aggregated protein (on 1 micron magnetic Sera-Mag beads; GE Healthcare) was then digested with proteomics-grade trypsin (1:75 w/w ...
-
bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).
-
bioRxiv - Cell Biology 2020Quote: siRNA-mediated knockdown in 786-O cells was carried out using 5-10 nM Dharmacon ON-TARGETplus siRNAs (GE Healthcare), diluted in siRNA buffer and mixed with DharmaFECT transfection reagent (1:50 in optiMEM reduced serum media) ...
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant was filtrated through a O.22μm pore filter and loaded on a 5 ml pre-packed Hitrap Q FF column (GE Healthcare) equilibrated with buffer A (50mM bis-tris-HCI pH 6.0 ...
-
bioRxiv - Microbiology 2021Quote: ... The primary antigen-antibody complex was detected by incubating the blot with goat anti-rabbit IgG HRP (1:10000, Jackson-ImmunoLab) and sheep anti-human IgG HRP (1:5000, GE Healthcare) in 3% milk in PBS-T ...
-
bioRxiv - Microbiology 2020Quote: ... Purification of the 4D11 mAb was carried out by affinity chromatography using a Protein A/Protein G GraviTrap 1-mL column (GE Healthcare), following the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... by injecting 50 µL of 8 µM protein in Protein Buffer onto a 1 mL HiTrap Heparin HP column (GE Healthcare) and eluting with a 10 CV linear gradient from 150 mM – 1 M NaCl in 20 mM Tris pH 7.5 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Immunoglobulins were purified against Protein A from 1 ml of rabbit serum using a Protein A HP SpinTrap (28-9031-32, GE Healthcare) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2021Quote: ... Precipitation of radiolabeled SARS-CoV-2 Spike glycoproteins from cell lysates or supernatant was performed with CV3-25 in combination with a polyclonal rabbit antiserum raised against SARS-CoV-2 RBD protein for 1 h at 4°C in the presence of 45 μL of 10% protein A-Sepharose beads (GE Healthcare).
-
bioRxiv - Biophysics 2019Quote: ... After dilution of the protein sample to a DTT concentration of 1 mM the reduced protein were immobilized on a Ni2+-Sepharose resin (GE Healthcare) and washed with ten column volumes of buffer A (50 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2019Quote: ... Extracts were precleared for 1 h with protein A-Sepharose (GE Healthcare) supplemented with 1mg/ml BSA ...
-
bioRxiv - Immunology 2020Quote: Columns of 1 ml Protein G sepharose 4 fast flow (GE Healthcare) were prepared and equilibrated with 5 volumes of binding buffer (0.02 M sodium phosphate ...
-
bioRxiv - Microbiology 2020Quote: Proteins were eluted directly into a 1 mL HisTrap column (GE Healthcare) using purification buffer supplemented with Desthiobiotin 2.5 mM (IBA Solutions For Life Science™) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Proteins were detected with HRP-conjugated secondary antibodies (1:10,000; GE Healthcare) and visualized using an AI600 Imager (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins were applied to a 1 ml HisTrap HP column (GE Healthcare), pre-equilibrated with buffer I (40 mM Tris-HCl ...
-
bioRxiv - Systems Biology 2023Quote: ... Proteins were isolated to magnetic beads (1 micron SeraMag beads; GE Healthcare) by the protein aggregation capture (PAC ...
-
bioRxiv - Microbiology 2022Quote: ... Virus stocks were cleared of residual EdC using a PD-10 desalting column (GE Healthcare, Mississauga, ON) utilizing the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... The virus-inactivated RPC eluate was loaded on to 0.10 L Macrocap SP Sepharose (GE Healthcare, USA) column to remove RPC buffers and rhEPO aggregates followed by reconstitution into pre-formulation buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... the pre-cleaning step was omitted and the ChIP extract directly incubated o/n on a rotor at 4°C with 50 μl IgG Sepharose 6 Fast Flow beads (GE Healthcare).
-
bioRxiv - Molecular Biology 2021Quote: ... The lysate was incubated with Glutathione Sepharose beads (20 μL for one group, 17-0756-01, GE healthcare, Sweden) at 4 °C overnight ...
-
bioRxiv - Microbiology 2019Quote: LecB was labeled with fluorescent dyes bearing NHS esters as reactive groups (Cy3 mono-reactive NHS ester (GE Healthcare), Cy5 mono-reactive NHS ester (GE Healthcare) ...
-
bioRxiv - Neuroscience 2021Quote: ... Control human blood samples were processed to remove the most abundant proteins using albumin and IgG depletion SpinTrap columns (GE Healthcare Cat 28-9480-20) following the manufacturers instructions ...
-
bioRxiv - Biochemistry 2022Quote: The cDNA of human full-length SMN and truncations were inserted in pGEX-6P-1 (GE Healthcare) using BamHI and XhoI ...