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Citations for GE Life Sciences :
201 - 250 of 274 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Human serum samples were heat inactivated at 56°C for 1 h and then incubated with an equal volume of Protein G resin (GE Healthcare) for 3 nights at 4°C on a rocking platform ...
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bioRxiv - Immunology 2024Quote: ... NHP serum samples were heat inactivated at 56°C for 1 h and then incubated with an equal volume of Protein A resin (GE Healthcare) for 3 nights at 4°C on a rocking platform ...
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bioRxiv - Cell Biology 2024Quote: ... cleared extracts were alkylated twice with 20 mM iodoacetamide (3 h at RT in the dark) and then cleaned by two passages on PD-SpinTrap G-25 columns (GE Healthcare). Samples were then clicked as explained before ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by centrifugation at 50,000⍰×⍰g for 1⍰h at 4⍰°C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) and the protein concentration was determined using a 2-D Quant kit (GE Healthcare, Piscataway NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Duplicate 120 ml water samples were filtered serially through 2.7 μm Whatman GF/D (GE Healthcare, Little Chalfort, UK) and 0.22 μm Sterivex (Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... for 1 h at 37 °C and the RNA was desalted twice by Illustra Microspin G-25 columns (GE Healthcare Life Sciences). Method for EMSA was modified from a previously published protocol (30) ...
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bioRxiv - Molecular Biology 2020Quote: ... with Tris-buffered saline/Tween-20 was followed by incubation for 1 h at room temperature with the appropriate secondary antibody (Anti-mouse IgG horseradish peroxidase cat# NA931V from GE Healthcare, Uk). Western blots were developed using the ECL Prime western blotting detection Reagent (RPN 2232 ...
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bioRxiv - Bioengineering 2021Quote: ... at 25 mA for 1 h and transferred onto an Amersham™ Protran® 0.2 μm nitrocellulose blotting membrane (GE Healthcare, Canada) at 300 mA for 1.5 h ...
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bioRxiv - Cell Biology 2021Quote: ... human ECs were transfected twice (at 0 and 24 h) with 200 pmol of siGENOME Non-Targeting siRNA #1 (as control) or siGENOME SMART pools siRNA oligonucleotides (GE Healthcare Dharmacon), using Oligofectamine Transfection Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... The suspension was incubated up to 1 h and the non-coupled free dye was removed by exclusion chromatography using a PD10 exclusion column (GE Healthcare, Sweden) equilibrated with 50 mM NaH2PO4/Na2HPO4 with 1% glycerol.
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated secondary antibodies were incubated for 1 h at room temperature and membrane development was performed using Enhanced Chemiluminescence (ECL) Western Blotting substrate (GE healthcare, RPN2106) or ECL Prime Western Blotting substrate (GE healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... The immunoblots were washed and incubated for 1 h at RT with HRP-linked secondary antibody donkey anti-rabbit IgG (GE Healthcare, NA934). The HRP-signal was detected using SuperSignalTM West Pico PLUS Chemiluminescent Substrate Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies coupled to horseradish peroxidase (HRP) were applied for 1 h: sheep anti-mouse (#NA9310V, GE Healthcare, 1:4000), donkey anti-rabbit (#NA9340V ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then collected by vacuum filtration onto glass microfiber filters (grade GF/C, 125 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 50 mL centrifuge tubes (Sarstedt ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was washed 5 times in PBS-T before it was incubated with the secondary antibody goat α-mouse IgG (H&L)-HRP (1:10’000, GE healthcare #NXA931). After 1 h incubation ...
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bioRxiv - Neuroscience 2022Quote: ... Images were captured one frame every 1 min 30 sec over 5 h using a DeltaVision Imaging System (Applied Precision, Bratislava, Slovakia).
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bioRxiv - Microbiology 2022Quote: ... were collected by gentle vacuum filtration onto glass microfiber filters (grade GF/A, 47 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman), immediately plunged into liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then collected by gentle vacuum filtration of 1-2 L onto glass microfiber filters (grade GF/A, 47 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 2.0 mL centrifuge tubes (SafeLock ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins in the strip were separated by SDS-PAGE (200 V, 5 h) and the gel was imaged using a Typhoon 9500 fluorescence imager (GE Healthcare, Germany) at a resolution of 100 μm/pixel for Cy2 at 488 nm excitation ...
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bioRxiv - Cell Biology 2022Quote: ... Extract was cleared by centrifugation at 186.000 x g for 1 h at 4°C and then incubated at 4°C with Glutathione Sepharose™ 4B resin (GE Healthcare) for 3h ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 W for 2–2.5 h) followed by detection on an Amersham Typhoon Gel and Blot Imaging System (GE Healthcare Life Sciences).
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bioRxiv - Molecular Biology 2024Quote: ... the gel was exposed to phosphor imager screen (Fujifilm Bas cassette2, Japan) for 4 h followed by imaging with Typhoon 9210 phosphor imager (GE Healthcare, USA) and Azure Sapphire (Azure Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with 1-3 nM non-targeting siRNA as a control (D-001810-10-05, GE Healthcare Dharmacon) or with siRNAs targeting PDK2 and PYCR1 (Dharmacon ...
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bioRxiv - Plant Biology 2021Quote: ... The dried seeds were placed on two layers of glass microfiber filters (Whatman GF/D, GE Healthcare, Chicago, IL, USA) moistened with distilled water in a Petri dish in the dark at 23 °C for a week (conditioning) ...
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bioRxiv - Neuroscience 2023Quote: ... Knockdown experiments involved the use of Accell non-targeting control siRNA (D-001910-01, GE Healthcare Dharmacon, Lafayette, CO, USA) and Accell mouse Rbfox1 siRNA (A-041929-13 ...
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bioRxiv - Biochemistry 2022Quote: ... ON-TARGETplus SMART Pool Non-targeting (D-001810-10) and CHMP2A (L-020247-01) siRNAs were obtained from GE Healthcare Dharmacon ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed 3 times for 5 min in TBST before incubation for 1 h at room temperature with secondary antibodies: donkey anti-mouse (1:5000; GE Healthcare, NA931-1ML), or donkey anti-rabbit (1:5000 ...
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bioRxiv - Plant Biology 2021Quote: ... Radioactive gels were exposed to a BAS phosphor-imaging plate (TR2040, Fuji) for 24 h and visualised using a Typhoon scanner (GE Healthcare, FLA-9500).
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were immunoprecipitated with the indicated antibodies for 3 h at 4 °C and then incubated with protein G (GE Healthcare Life Sciences) for 2 hrs at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... The mixtures were incubated at 4°C for 1 h prior to purification on a Superdex 200 Increase SEC column (GE Healthcare Life Sciences) in buffer containing 150mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... lysate was recovered and incubated for 2 h with pre-equilibrated Ni-NTA beads (Ni Sepharose 6 Fast Flow, GE Healthcare Life Sciences) in a binding buffer (50 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... was achieved through incubation at RT for 1 h and subsequent size-exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare, Pittsburgh, USA), equilibrated in storage buffer (50 mM HEPES pH 7.25 ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and with KCl to a final concentration of 0.3 M and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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Rhodococcus equi Escapes Macrophage Autophagy: VapA Inhibits ATP6V0d1-Mediated Lysosomal DegradationbioRxiv - Immunology 2023Quote: ... second antibody (1:10000) for 1 h at room temperature and then proteins were visualized with enhance chemiluminescence (ECL) kit purchased from GE Healthcare (Chicago, USA). And detection reagent and analyzed using GE Amersham Imager 600.
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of purified DNA was labelled for 3 h at 15°C with fluorescent dUTPs (SpectrumOrange and SpectrumGreen from Abott Molecular and Cy5-UTPs from GE HealthCare Life Science).The templates used in this study are ...
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bioRxiv - Biochemistry 2023Quote: ... TLC plates were visualized via exposure to a phosphor screen for 16 h prior to imaging using a phosphor screen and Typhoon FLA7000 (GE Healthcare, Pittsburgh PA). Silica gel plates used to assay the metabolism samples and lesion extracts were 150 Å Silica Gel HL 250µ m 10 x 20 cm channeled plates with a pre-adsorbent zone purchased from Analtech/iChromatography (Newark ...
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were incubated for 2 h at 25°C and then filtered through a Protran nitrocellulose membrane (Whatman, GE Healthcare Bioscience, Pittsburgh, PA) on top of a Hybond-XL (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were precipitated the supernatant by addition of ammonium sulfate to 67% saturation and dissolved in buffer D and loaded on equilibrated 5 mL HiTrap Heparin HP sepharose column (GE Healthcare). The protein was eluted with a linear 0-1 M NaCl gradient in buffer D ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Microbiology 2023Quote: ... centrifuged 30 min at 2150g and concentrated/diafiltrated against loading buffer (D-PBS, 500 mM NaCl, 5 mM Imidazole) using the AKTA crossflow system (GE healthcare) supplemented with a 10KDa ...
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bioRxiv - Microbiology 2023Quote: ... M-003854-01-0005) and a “NonTargeting” (SMARTpool siGENOME NonTargeting, D-001210-04-20) control siRNAs were obtained from GE Healthcare Dharmacon (Lafayette ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant clarified from the cell lysate after overnight induction by 0.5mM isopropylthio-β-D-1-galactopyranoside (IPTG) at 20 °C was applied to pre-equilibrated 5Lml HisTrap column (GE Healthcare). After thorough wash with binding buffer (20LmM Tris–HCl pHL7.4 ...
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bioRxiv - Neuroscience 2024Quote: ... coli B21 Gold cells using 0.5 mM isopropyl thio-β-D-galactoside (IPTG) and purified on a NiNTA column (QIAGEN, ÄKTA Purifier, GE Healthcare) at 4 °C as described previously (2) ...
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bioRxiv - Neuroscience 2020Quote: ... in which they were precleared for 1 h using 30 μl (dry volume) of a 1:1 mixture of protein-A and protein-G-agarose beads (GE Healthcare, Chicago, IL, USA). Thereafter ...
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bioRxiv - Neuroscience 2021Quote: ... IRDye® 680LT goat anti-rabbit IgG (H + L) (LiCor, cat. #926-6802, 1:10,000) and horseradish peroxidase (HRP)-conjugated goat anti-chicken IgG (GE Healthcare, cat. #AS09603, 1:10,000).
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bioRxiv - Biochemistry 2021Quote: ... The reaction was performed at 37 °C for 16 h and terminated by desalting it to 50 mM ABC buffer with HiTrap Desalting column (GE Healthcare: Chicago, IL, USA). It was further purified using Ni-NTA resin and collected the flow-through to remove the side product ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times with PBST for 5 min and incubated for 1 h at room temperature with HRP-conjugated secondary anti-rabbit IgG (GE Healthcare, NA934, 1/2000) or anti-mouse IgG (Amersham ...
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bioRxiv - Microbiology 2022Quote: ... were collected by vacuum filtration onto glass microfiber filters (grade GF/C, 47 mm in diameter, pre-combusted at 460°C for >8 h, GE Healthcare Whatman, Buckinghamshire, UK), immediately plunged into liquid nitrogen ...