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Citations for GE Life Sciences :
1 - 50 of 274 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Microbiology 2021Quote: The Alumina Membrane Chambers (AMCs) for 13 mm alumina Whatman Anodisc membranes with 0.2 μm pores (GE Healthcare, Little Chalfont, UK) were designed in-house using FreeCAD v0.19 (https://www.freecadweb.org) ...
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bioRxiv - Plant Biology 2022Quote: ... induced for 3 h induction by IPTG (isopropyl b-D-thiogalactopyranoside) and soluble extracts were purified using GST-sepharose 4B (GE Healthcare, Sweden) as described elsewhere (Geisler et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactivity was detected using species-appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000; GE Healthcare, Cleveland, OH, USA). Western blots were imaged via HRP chemiluminescent detection (Azure Biosystems ...
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bioRxiv - Immunology 2021Quote: ... Finkelman (University of Cincinnati, OH) (20) and purified from culture filtrates by standard protein G column chromatography (Amersham Biosciences/GE Healthcare, Piscataway, NJ). The hybridoma cells were grown in RPMI-1640 medium (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... 2-D and 3-D images were obtained and analyzed using the software program MicroView (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Cell Biology 2021Quote: ... the siRNA D-023324-05 (GE Healthcare Dharmacon) and Stealth RNAis (#HSS126645 ...
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bioRxiv - Neuroscience 2020Quote: ... the 2-D Clean-Up Kit (GE Healthcare) was used following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The probe (L3-12-D, GE Healthcare Japan) was applied to the target site with the upper surface of the echo image facing the knee joint and the lower surface facing the ankle joint ...
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bioRxiv - Cell Biology 2023Quote: ... and treated for 4 h with PreScission protease (GE Healthcare). The TAP-ESP beads were used directly ...
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bioRxiv - Cancer Biology 2021Quote: Approximately 500,000 cells were transfected using a final concentration of 20 nM EPSTI1 (J-015094-09-0020 and J-015094-12-0020) or non-targeting (D-001810-01-20 and D-001810-02-20) ON-TARGETplus siRNAs from GE Healthcare Dharmacon using 20 µL of Lipofectamine RNAiMAX (ThermoFisher #13778-150 ...
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bioRxiv - Cell Biology 2022Quote: ... Cat# 015094-12-0020) or non-targeting (D-001810-01-20 and D-001810-02-20) ON-TARGETplus siRNAs from GE Healthcare Dharmacon using 20 μL of Lipofectamine RNAiMAX (ThermoFisher #13778-150 ...
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bioRxiv - Cell Biology 2023Quote: ... D) were deconvolved using Softworx (Applied Precision; Rača, Slowakia). Images acquired from LSM 710 confocal microscope were deconvolved using Huygens Software (Scientific Volume Imaging ...
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bioRxiv - Microbiology 2022Quote: ... at a dilution of 1:10,000 for 1 h (GE Healthcare). Proteins were detected using SuperSignal West Femto substrate (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... for 1 h before centrifugation on a discontinuous Percoll gradient (GE Healthcare). Isolated cells were then used in various cell function assays
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bioRxiv - Microbiology 2020Quote: ... and secondary antibodies α-mouse IgG (H&L)-HRP (GE healthcare #NXA931), 1:5000 and α-rabbit IgG (H&L)-HRP (GE Healthcare #NA934) ...
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bioRxiv - Microbiology 2020Quote: ... 1:5000 and α-rabbit IgG (H&L)-HRP (GE Healthcare #NA934), 1:5000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA species were transferred onto a Hybond-H+ membrane (GE Healthcare) in 1X TBE buffer and crosslinked by UV exposure for 2 minutes upon electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h in combination with ECL (GE Healthcare, Chicago, IL, USA). Image detection was performed with C600 Azure Biosystems Imager (Biozym)/ChemiDoc XRS System (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... Cells were imaged 20-28 h post-infection on a Typhoon (GE Healthcare) or Sapphire (Azure ...
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bioRxiv - Developmental Biology 2021Quote: ... were coupled 2 h at RT to protein A Sepharose beads (GE Healthcare). Coupled beads were covalently crosslinked using dimethyl pimelimidate according to standard procedures ...
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bioRxiv - Molecular Biology 2022Quote: ... H-NS C21S was then applied to a HiTrap Hep column (GE Healthcare) and eluted with Heparin Buffer containing 1 M NaCl ...
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bioRxiv - Biophysics 2021Quote: ... before incubation 2 h with 500 μL CNBr-activated Sepharose beads (GE Healthcare) coupled to monoclonal Rho-1D4 antibody (University of British Columbia ...
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bioRxiv - Cell Biology 2024Quote: ... or goat α-mouse IgG (H&L)-HRP (1:10,000, GE healthcare #NXA931), respectively ...
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bioRxiv - Biophysics 2024Quote: ... 17 h) and size exclusion chromatography (Superdex 200 10/300 GL (GE Healthcare)) as previously described34.
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bioRxiv - Plant Biology 2020Quote: ... Protein quantification was performed with 2-D Quant kit (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined with 2-D Quant kit (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Control siRNA (negative) was obtained from GE Healthcare (D-001810-10).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We used the 2-D Quant Kit (GE Healthcare, Uppsala, Sweden) to determine protein concentrations.
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bioRxiv - Immunology 2023Quote: ... Contaminants were removed with 2-D Clean-up kit (GE Healthcare), and in-tube trypsin digestion was performed ...
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bioRxiv - Cancer Biology 2022Quote: ... for 4 h in T cell culture media composed of RPMI1640 (GE Healthcare, SH30027.01) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... for 2 h and then incubated with Streptavidin Sepharose High Performance beads (GE Healthcare) for 4 h ...
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bioRxiv - Cancer Biology 2020Quote: ... or scramble siRNA pool (GE Healthcare Dharmacon, Catalog# D-001206-14-05) were mixed with Opti-MEM medium and Lipofectamine 3000 (L3000001 ...
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bioRxiv - Cell Biology 2021Quote: ... a non-targeting scramble siRNA (#D-001210-01-05, GE Healthcare Dharmacon) was used in the experiments shown in figure 3 and supplementary figure 6C while a pool of four scrambled siRNAs (#D-001206-13-05 ...
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bioRxiv - Cell Biology 2022Quote: ... Non-targeting siRNA ON-TARGETplus (D-001810-10-05) (Dharmacon, GE Healthcare) served as the siRNA control (“siRNA scramble”) ...
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bioRxiv - Biophysics 2022Quote: ... cells at 32°C with 0.4 mM Isopropyl-D-thiogalactoside (GE Healthcare) for 8 h ...
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bioRxiv - Cell Biology 2021Quote: ... and then for another 1 h following the addition of protein-G Sepharose (GE Healthcare). Protein bound to antibody beads was washed five times with lysis buffer at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1 h at room temperature and visualized using Enhanced Chemo-Luminescence (ECL) (GE Healthcare) or Typhoon Biomolecular Imager (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred at 70 V for 2 h onto a nitrocellulose membrane (GE Healthcare) using a wet electroblotting system (BIO-RAD) ...
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bioRxiv - Cell Biology 2023Quote: ... The cleared lysate was incubated for 2 h with Glutathione 4B Sepharose (GE Healthcare, Germany) followed by washing of the Sepharose with 50 mM HEPES-NaOH ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 1 h of incubation with protein A–Sepharose CL-4B beads (GE Healthcare). Immunoprecipitates were washed three times in lysis buffer ...
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bioRxiv - Biophysics 2023Quote: Suspension HEK HEK293S GnTI- cells were grown and maintained in HyClone TransFx-H (GE Healthcare) media supplemented with 1% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... for approximatively 3 h and transferred to polyvinylidene fluoride membranes (GE Healthcare, Velizy-Villacoublay, France) in cold blotting buffer (25mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred at 70 V for 2 h onto a nitrocellulose membrane (GE Healthcare) using a wet electroblotting system (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using 2-D quant kit (GE healthcare, Cat# 80648356), by following manufacturer’ instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... H-NS containing cell lysate was directly applied to a HiTrap Heparin HP column (GE Healthcare) equilibrated with Binding Buffer with 130 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Incubation using the secondary antibody α-mouse IgG (H&L)-HRP (1:10,000) (GE healthcare #NXA931) diluted in blocking buffer was performed for two hours ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were mixed 2 h at 4°C with Protein-A-conjugated sepharose beads (GE Healthcare) and anti-Myc antibody (9B11 ...
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bioRxiv - Cell Biology 2022Quote: ... and Donkey anti-rabbit IgG(H+L) antibody conjugated with HRP (GE Healthcare, NA934V, 1:10,000). Antibody signals were detected using SuperSignal West Pico Plus Chemiluminescent Substrate (Thermofisher ...