Labshake search
Citations for GE Life Sciences :
201 - 250 of 6168 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins recognised by the antibodies were visualised using ECL prime (GE Healthcare, Chicago, IL, USA) and digital images were acquired with a LAS4000 mini imager (FUJIFILM Wako Pure Chemical ...
-
bioRxiv - Microbiology 2021Quote: ... IgG1 antibodies were isolated from cell supernatant using a HiTrap protein A column (GE Healthcare) and Äkta Pure protein chromatography system (GE Healthcare) ...
-
bioRxiv - Immunology 2021Quote: ... IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva, GE Healthcare). Antibodies were dialyzed overnight in PBS and filter-sterilized though 0.22 µm Spin-X filters ...
-
bioRxiv - Microbiology 2022Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
-
bioRxiv - Immunology 2023Quote: ... the recombinant monoclonal antibodies were purified from the supernatant with protein G beads (GE Healthcare).
-
bioRxiv - Biochemistry 2023Quote: ... The antigen–antibody complexes were pulled down by 30 μl Protein A-Sepharose (GE Healthcare) and specific proteins were detected by the Western blotting analysis ...
-
bioRxiv - Immunology 2023Quote: ... Antibodies were purified from clarified supernatants using Protein G Sepharose 4 Fast Flow (GE Healthcare), dialyzed in PBS ...
-
bioRxiv - Immunology 2024Quote: ... antibodies in the supernatant were collected and captured by Magnetic Protein A-Sepharose (GE Healthcare). Bound antibodies were eluted by solution buffer Glycine pH 2.0 and further purified by gel filtration chromatography using a Superdex 200 High Performance column (GE Healthcare) ...
-
bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
-
bioRxiv - Molecular Biology 2020Quote: ... The fractions containing the protein were pooled and applied onto a size-exclusion chromatography column (HiLoad 26/600 Superdex 200 pg, GE Healthcare) pre-equilibrated with lysis buffer ...
-
bioRxiv - Plant Biology 2020Quote: ... The protein was eluted with IMAC buffer containing 330 mM imidazole and loaded onto a HiTrap SP HP column (GE Healthcare). The column was washed with ion exchange buffer (IEC ...
-
bioRxiv - Biochemistry 2021Quote: ... Pooled fractions containing target protein was subjected to buffer exchange with buffer A using a HiPrep 26/10 desalting column (GE Healthcare). Next ...
-
bioRxiv - Biochemistry 2020Quote: ... The flow-through fraction containing the target protein was further purified by gel filtration using a Superdex 200 10/60 GL column (GE Healthcare) equilibrated with the storage buffer (10 mM HEPES ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein containing fractions were pooled and passed through an ion exchange column (HiTrap® SP FF GE Healthcare Life Sciences) pre-equilibrated in the SP buffer A (25 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2020Quote: ... Fractions containing the recombinant proteins were pooled and injected into a size-exclusion chromatography column (Superose12 10/300 GL; GE Healthcare) equilibrated with 50 mM Bis-Tris pH 6.5 ...
-
bioRxiv - Plant Biology 2021Quote: ... the eluates containing recombinant proteins with 10× His-MsyB tag were further purified by an anion-exchange column (Source-15Q; GE Healthcare). The 10×His-MsyB tag was cleaved by TEV protease at 4 °C overnight and removed by an anion-exchange column ...
-
bioRxiv - Biochemistry 2021Quote: ... Protein containing elution fractions were concentrated on Amicon concentrator (100 kDa MWCO) and loaded a Superose 6 16/600 (GE Healthcare) pre-equilibrated in SEC buffer (50 mM HEPES pH 7.5 ...
-
bioRxiv - Biophysics 2021Quote: ... was purified by 1 M NaCl containing Tris buffer using AKTATM Pure protein purification system with Hi-Trap Q-FF anion exchange column (GE healthcare). 0.3% H2O2 was added to the purified proteins ...
-
bioRxiv - Microbiology 2021Quote: ... Peak fractions containing desired proteins were pooled and further purified by size exclusion chromatography (HiLoad 16/60 Superdex 200 column, GE Healthcare) using sodium phosphate buffer A as eluent ...
-
bioRxiv - Microbiology 2021Quote: ... Bound protein was eluted with elution buffer (lysis buffer containing 250 mM imidazole) and loaded onto glutathione Sepharose resin (GE Healthcare) to separate bound NEC from excess His6-SUMO-UL31 ...
-
bioRxiv - Cell Biology 2022Quote: ... Fractions containing the proteins were pooled and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer ...
-
bioRxiv - Microbiology 2022Quote: ... A total of 100 μl containing 10 mg/ml of disassembled protein were loaded onto a Superdex 75 Increase 10/300 GL (GE Healthcare) using 300 mM NaCl for elution ...
-
bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) preequilibrated with the HiTrap binding buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) pre-equilibrated with the HiTrap binding buffer ...
-
bioRxiv - Immunology 2023Quote: ... Protein-containing fractions were combined and purified further via SEC on a Superdex 75 10/300 GL column (GE Healthcare/Cytiva) using 20 mM sodium phosphate buffer ...
-
bioRxiv - Biophysics 2023Quote: ... The fractions containing the protein of interest were concentrated and further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
-
bioRxiv - Microbiology 2024Quote: ... The fractions containing L–P protein were further purified using a size-exclusion column (Superose 6 Increase 10/300 GL, GE healthcare) equilibrated with size exclusion chromatography (SEC ...
-
bioRxiv - Biochemistry 2024Quote: ... The elutions containing the proteins were loaded onto a HiLoad 16/600 Superdex 75 or HiLoad 16/600 Superdex 200 column (GE Healthcare) pre equilibrated with SEC buffer (10 mM HEPES pH 7.5 ...
-
bioRxiv - Bioengineering 2022Quote: ... the receptor fragment MET567 comprising the N-terminal SEMA domain and the cysteine-rich/PSI domain was immobilized at low-density (515 RU) using amine-coupling on a CM5 sensor chip (GE Healthcare). A blank inactivated reference channel was used to correct for non-specific binding ...
-
bioRxiv - Biochemistry 2020Quote: ... REC3 domain (WT and R691A mutant) were further purified by size-exclusion chromatography on a Superdex 200 16/60 column (GE Healthcare) in 20 mM Tris ...
-
bioRxiv - Microbiology 2020Quote: ... The methylated VSG13 N-terminal domain was purified by gel filtration on a Superdex 200 Increase 10/300 GL (GE Healthcare) in 10 mM Tris.Cl ...
-
bioRxiv - Immunology 2020Quote: ... the surface was saturated with two sequential injections of a 20 μM knob domain solution in HBS-EP buffer (GE healthcare), using a flow rate of 30 μL/min and contact time of 300 seconds ...
-
bioRxiv - Microbiology 2022Quote: ... The N- and C-terminal domains were separated by size exclusion chromatography using HiLoad 16/600 Superdex 200 column (GE Healthcare) in AKTA pure chromatography system (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2023Quote: Antibodies were expressed by transient transfection in Expi-CHO cells as murine IgG2a Fc domain conjugates and purified using the HiTrap MabSelect SuRe (GE Healthcare) affinity chromatography ...
-
bioRxiv - Biochemistry 2022Quote: ... The SAFB2 SAP and RRM domains were further purified via size exclusion on a HiLoad 16/600 SD 75 (GE Healthcare), respectively ...
-
bioRxiv - Biochemistry 2023Quote: ... and the phosphorylated kinase domain of FGFR1 was collected after size exclusion chromatography purification using Superdex 75 10/300 GL column (GE Healthcare). To prepare the dephosphorylated form ...
-
bioRxiv - Molecular Biology 2024Quote: ... and pGEX-Spinophilin(PDZ) plasmids were obtained by cloning Neurabin and Spinophilin PDZ domains into the pGEX 6P2 vector (GE Healthcare). The PP1-4E-BP1 chimera was generated by insertion of 4E-BP1 sequences into pET28-PP1.
-
bioRxiv - Biophysics 2024Quote: ... Pro-TGF-β1 was produced in HEK293 suspension culture and mature growth factor was purified from pro-domain by high-resolution cation–exchange chromatography on MonoS column (GE Healthcare).
-
bioRxiv - Synthetic Biology 2020Quote: ... Aggregated protein (on 1 micron magnetic Sera-Mag beads; GE Healthcare) was then digested with proteomics-grade trypsin (1:75 w/w ...
-
bioRxiv - Bioengineering 2020Quote: ... for HRP-conjugated antibodies: donkey monoclonal anti-mouse (1:10000) and donkey monoclonal anti-rabbit antibody (1:10000, GE Healthcare) diluted in 2% BSA-TBST ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-Rabbit secondary antibody (GE healthcare,1:10000). Membranes were developed by the Odyssey Fc Imaging system (LI-COR Bioscience).
-
bioRxiv - Cell Biology 2020Quote: ... Fractions containing CLIK-1 were applied to HiLoad 16/60 Superdex 75 (GE Healthcare) and eluted with a buffer containing 0.1 M KCl ...
-
bioRxiv - Biochemistry 2021Quote: ... 0.1mg of purified antibody was injected into the AKTA protein purification system (GE Healthcare Life Sciences) and protein fractions were separated using a Superdex 200 10/300 column (GE Healthcare Life Sciences ...
-
bioRxiv - Immunology 2020Quote: ... the antibodies secreted into the supernatant were collected and captured by protein A Sepharose (GE Healthcare). The bound antibodies were eluted and further purified by gel-filtration chromatography using a Superdex 200 High Performance column (GE Healthcare) ...
-
bioRxiv - Neuroscience 2022Quote: ... Ribosomes bound to an anti-HA antibody was purified using protein G magnetic beads (GE Healthcare). The RNA bound to ribosomes was purified with RNeasy Protect Mini Kit (QIAGEN ...
-
bioRxiv - Immunology 2022Quote: ... the supernatants consisting of secreted antibodies were collected and trapped by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and concentrated using Centricon Plus-70 (051555 ...
-
bioRxiv - Microbiology 2022Quote: ... The supernatants were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
-
bioRxiv - Immunology 2020Quote: ... The secreted antibodies were subsequently purified from the supernatant on HiTrap Protein G columns (GE Healthcare). Plasmid DNA encoding a gliadin-reactive IGHV4-4:IGKV4-1 antibody with R in position 55 (corresponding to the IGHV4-4*07 allele ...
-
bioRxiv - Neuroscience 2021Quote: ... The supernatant was concentrated and purified over HiTrap Protein G HP antibody purification column (GE Healthcare). To visualize 410C9 in vivo ...
-
bioRxiv - Immunology 2023Quote: ... The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe, GE Healthcare), followed by a preparative size exclusion chromatography (SEC) ...