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Citations for GE Life Sciences :
1 - 50 of 6168 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... MnCl2 or CaCl2was titrated into a sample cell containing 0.1 mM catalytic domain of the g12777 protein using an iTC200 calorimeter (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... The purified proteins or domains were immobilized on CM5 chip (GE Healthcare, USA) at response unit 2914.6 ...
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bioRxiv - Microbiology 2023Quote: ... the proteins and domains were purified by ÄKTA Go System (GE Healthcare, USA) with Ni-NTA affinity chromatography column (HisTrap HP ...
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bioRxiv - Cell Biology 2022Quote: The real time protein-protein interaction of protease and PDZ domain proteins of PfHtrA2was analysed by SPR using Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) as described previously [7] ...
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bioRxiv - Neuroscience 2021Quote: ... the E domain was subcloned into pGEX-6P-1 (GE healthcare). The GST-gephyrin E domain fusion protein was purified from E ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants containing antibodies were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
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bioRxiv - Bioengineering 2020Quote: ... The antibody expression vectors were co-transfected into 293F cells and the cell culture supernatants containing secreted antibody IgG were purified by columns packed in-house containing Protein A Sepharose beads (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The α-domains of the proteins were purified to homogeneity by size exclusion chromatography (Superdex 75 GE Healthcare) equilibrated in 25 mM HEPES pH 7 ...
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bioRxiv - Microbiology 2021Quote: GST alone and GST-tagged WWOX WW1 and WW2 domains fusion protein were expressed in BL-21 cells and subsequently purified and conjugated to glutathione (GSH) beads (GE HEALTHCARE). HEK293T cells were transfected with eVP40-WT ...
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bioRxiv - Microbiology 2022Quote: ... antibodies diluted 1:500 in 1 U/mL DNAse I in Tris Buffered Saline containing 1% BSA (GE Healthcare). Three 5-minute washes with block (1% BSA with 0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were detected with HRP-conjugated secondary antibodies (1:10,000; GE Healthcare) and visualized using an AI600 Imager (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing the tagged domain were pooled and exchanged to the initial buffer containing 5 mM imidazole by using a PD10 desalting column (GE Healthcare Life Sciences). His-tagged TEV protease (0.1 mg/ml final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... CALM ANTH domain and Ub proteins were further purified on a HiLoad 16/60 Superdex 200 column (GE Healthcare Biosciences, Pittsburg, PA) equilibrated with 20mM HEPES-NaOH ...
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bioRxiv - Immunology 2021Quote: ... extracellular domains were expressed in Drosophila S2 cells and purified by immunoaffinity chromatography with LB3.1 antibody followed by Superdex200 (GE Healthcare) size exclusion chromatography as described [65 ...
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bioRxiv - Microbiology 2020Quote: The heterologous proteins containing His-tags were purified via 1 ml Ni-NTA HisTrap columns (GE Healthcare). The cells were lysed in 50 ml lysis buffer containing 50 mM Na-phosphate buffer pH 8 ...
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bioRxiv - Synthetic Biology 2020Quote: The DNA fragment encoding the PreS1 domain (residues 1-56) was cloned into the pGEX-2T (GE Healthcare) plasmid ...
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bioRxiv - Biochemistry 2024Quote: ... The His-tag containing proteins were loaded onto a HiTrap chelating HP nickel affinity 1-ml column (GE Healthcare). The bound components were eluted with a gradient of 10–300 mM imidazole in 20 mM Tris pH 7.4 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... anti-EGFR antibody (#MI-12-1, MBL), anti-GFP antibody (#M-048-3, MBL) and Protein A-Sepharose (GE Healthcare) were used for immunoprecipitation ...
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bioRxiv - Biochemistry 2023Quote: ... Various Prp40 domains fused with an N-terminal Flag tag were cloned into pGEX-6p-1 vector (GE Healthcare) and purified from E.coli as GST fusion proteins ...
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bioRxiv - Biochemistry 2023Quote: ... The antibodies raised against each AtCYT6 domain were purified from the sera using CNBr-activated Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) with immobilized recombinant AtCYT6-NTD and AtCYT6-CTD according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Fractions containing pAgo proteins were loaded onto a Heparin column (GE Healthcare) equilibrated with buffer GF ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with TBST containing horseradish peroxidase-conjugated secondary antibody (1:1000; GE Healthcare, Pittsburgh, PA) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... HER2 domain IV was purified using a HisTrap excel column (GE Healthcare). The filtrated media were loaded into the HisTrap excel column ...
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bioRxiv - Biochemistry 2023Quote: ... for the PWWP domain or a HiTrap Q HP column (GE Healthcare) for the ADD domain ...
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected with protein-specific primary antibodies and HRP-coupled secondary antibodies by enhanced chemiluminescence (GE Healthcare) and imaged using X-ray films or with Fusion Capture Advance FX7 16.15 (Peqlab).
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted sample containing Pol I and Pol III protein fractions was loaded into a 1 mL Resource Q anion exchange column (GE Healthcare), which was then eluted ...
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bioRxiv - Developmental Biology 2021Quote: ... antibodies and protein G Mag sepharose (GE Healthcare) overnight at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... The coding sequences for the N-terminal (63-139 aa) and C-terminal (221-307 aa) domain of OR were cloned to the pGEX-4T-1 vector (GE healthcare) for prokaryotic expression ...
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bioRxiv - Cell Biology 2022Quote: GST or GST-tagged β’-COP (residues 1-604) or Glo3 constructs (GAP domain, BoCCS region, GRM region) were immobilized on glutathione sepharose resin (GE Healthcare) for one hour on ice ...
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bioRxiv - Microbiology 2021Quote: ... The protein-antibody complexes were captured with protein-G coupled Sepharose beads (GE Healthcare) by incubation at 4 °C for 1 h ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... Protein containing fractions were then loaded onto a Q HP column (GE Healthcare) and eluted from by a linear gradient of Q buffer A (100 mM Tris (pH 7.5) ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Immunology 2021Quote: ... Antibody was purified by Protein A Sepharose (GE Healthcare) as described previously (Sok et al. ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Immunology 2023Quote: ... Antibody was purified by Protein A Sepharose (GE Healthcare). Concentrations of purified mAbs was determined using a NanoDrop One (ThermoFisher).
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Physiology 2022Quote: ... The specific proteins were detected with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000 dilution; CtNo_NA934, GE Healthcare, 1:5000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing target protein were desalted from imidazole using PD10 desalting column (GE Healthcare) and incubated with 50 μM ONO-5430608 and a His-tagged PreScission protease (homemade ...
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bioRxiv - Cell Biology 2022Quote: ... The eluate containing recombinant proteins was passed through a PD-10 column (GE healthcare) to exchange buffer with Store Buffer (50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biophysics 2021Quote: ... Fractions containing the eluted protein were loaded onto a HiPrep desalting column (GE Healthcare) equilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Gel containing fluorescent protein samples were scanned with Typhoon FLA 9500 imager (GE Healthcare), using 473 nm blue LD laser/LBP (510LP ...
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bioRxiv - Neuroscience 2023Quote: ... and supernatant containing GST-hnRNPH2 proteins added to Glutathione Sepharose 4B (GSH; GE Healthcare) beads ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatant containing desired protein was applied to Glutathione-Sepharose resin (GE Healthcare Life Science) equilibrated with lysis buffer and agitated for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... The ARM domain was loaded on a Superose 6 Increase 10/300 (GE Healthcare) alone (at 2 mg/ml ...