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Citations for GE Life Sciences :
201 - 250 of 1867 citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... nuclei extracts from 3-week-old seedlings were transferred to a 0.2 μm nitrocellulose membrane (GE Healthcare) after separated by SDS-PAGE ...
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bioRxiv - Biochemistry 2024Quote: ... The filtered sample was incubated with 3 mL of CNBr activated Sepharose Fast Flow resin (GE Healthcare) beads coupled to GFP nanobody (42 ...
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bioRxiv - Genetics 2020Quote: ... Eluted fractions were diluted 3-fold with R buffer and loaded onto a HiTrap SP column (GE Healthcare). Rrp1 eluted at around 600 mM NaCl with a linear gradient of 0.1-1.0 M NaCl in R buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatant was transferred on a column containing 3 ml Ni-Sepharose High Performance resin (GE Healthcare), washed two times with lysis buffer containing 30 mM and 50 mM imidazole ...
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bioRxiv - Biophysics 2020Quote: ... zGDNFmat -zGFRα1D1-3 was further purified using size exclusion chromatography using a Superdex 200 (16/600) (GE Healthcare) in 20 mM Tris (pH 7.0) ...
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bioRxiv - Immunology 2020Quote: ... separated by SDS-PAGE using a 3-15% Tris-Acetate gel and transferred to nitrocellulose membranes (GE Healthcare). Densitometry analysis was performed by the Image J software (National Institutes of Health).
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bioRxiv - Immunology 2020Quote: Buffy coats were diluted [1:3] with PBS and added to Leukosep tubes containing Ficoll-Paque (GE Healthcare). Tubes were centrifuged at 450g for 40 minutes and the resulting buffy layer extracted ...
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bioRxiv - Neuroscience 2020Quote: ... age range: 20-29 years) were scanned in a 3-Tesla MRI scanner (DISCOVERY MR750, GE Healthcare, USA) using a 32-channel head-coil ...
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bioRxiv - Immunology 2020Quote: Whole-blood leukocyte DNA was extracted using the Nucleon BACC 3 DNA extraction kit (GE healthcare, Buckinghamshire, UK). We genotyped patients using the Illumina OmniExpressExome-8 Bead Chip (Illumina ...
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bioRxiv - Genomics 2022Quote: ... with an anti-rabbit IgG (goat polyclonal, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4301, RRID:AB_2650489) secondary antibody was used as a loading control ...
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bioRxiv - Neuroscience 2023Quote: ... MRI images were acquired with the aid of a 3-T scanner (Discovery 750; GE Healthcare, Milwaukee, WI) and an 8-channel head coil ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Membranes were washed with PBS-T for 3 × 10 min and were developed by ECL Prime (GE Healthcare) and imaged on a Biorad ChemiDoc MP Imaging System.
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bioRxiv - Immunology 2024Quote: ... The peak of properly refolded HLA-G2 was collected and buffer exchanged into HBS-EP (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA and 0.005% [v/v] Surfactant P20, GE Healthcare) for surface plasmon resonance (SPR ...
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bioRxiv - Biochemistry 2024Quote: ... Active fractions were pooled and concentrated using a 3-kDa molecular weight cutoff filter (Vivaspin 500, GE Healthcare), and used for further assays.
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bioRxiv - Biochemistry 2020Quote: ... Blots were then washed 3 times in TBS-T and bands visualized with Amersham ECL chemiluminescence reagent (GE Healthcare). Blots were stripped (62.5 mM Tris-HCl pH 6.5 ...
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bioRxiv - Genetics 2020Quote: ... then washed for 3 × 20 min in 1× SSC and 0.1% SDS and imaged using a phosphorimager (GE Healthcare) and scanned using an Amersham Typhoon 5 scanner ...
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bioRxiv - Bioengineering 2020Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
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bioRxiv - Cell Biology 2021Quote: ... SidK1-278-3×FLAG was further purified with a Superdex 200 10/300 Increase gel filtration column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.4 and 150 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Samples were concentrated L using a 3 kDa Vivaspin™ 20 filter column (GE Healthcare, Mississauga, ON, μ Canada). The conformational mixtures were purified via size exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2021Quote: ... 3 mM CaCl2 and 0.02% DDM) was injected onto a Superdex 200 Increase 10/300 GL column (GE Healthcare), equilibrated with buffer (25 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: Full-length mouse Csk (WT, H21I, K43D, and K222R3) were subcloned into a pGEX-6P-3 vector (GE Healthcare) containing an N-terminal glutathione S-transferase (GST ...
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bioRxiv - Microbiology 2021Quote: ... or denaturing 0.8% agarose gel for large RNA separation (0.2 to 3 kb) and transferred onto membrane Hybond N+ (GE Healthcare). RNAs were probed with γ32P 5’ end-labeled oligonucleotide (Table S3 ...
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bioRxiv - Neuroscience 2020Quote: Resting-state fMRI data were collected on a whole-body 3 Tesla MR750 MRI scanner (GE Healthcare, Milwaukee, WI) with an 8-channel receive-only head array coil at the Laureate Institute of Brain Research ...
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bioRxiv - Immunology 2020Quote: ... 3-fold serial dilution (spanning a range of 1 μM to 1.4 nM) in HBS-EP buffer (GE healthcare). A high flow rate of 40 μL/min was used ...
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bioRxiv - Bioengineering 2020Quote: ... at room temperature with HBS-EP+ running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). IgGs were immobilized on CM5 chips (GE Healthcare # 29104988 ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Isoelectric focusing (IEF) was performed using 7-cm Immobiline DryStrips (pH 3–11 nonlinear) (GE Healthcare Life Sciences, USA) in an Ethan IPGphor II Isoelectric Focusing System (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2022Quote: ... acute- and chronic-type ATL patients with 3% dextran and the density gradient medium Ficoll-Paque Plus (GE Healthcare). For lymphoma-type ATL ...
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bioRxiv - Neuroscience 2022Quote: ... and an aliquot of synaptosomal suspension (2 ml) was placed onto a 3-ml Percoll discontinuous gradient (GE Healthcare) containing (in mM ...
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bioRxiv - Microbiology 2024Quote: ... MV samples were administered at six concentrations (1:3 dilutions in Neurobasal A (Hyclone, GE Healthcare, Chicago, IL, USA) containing Glutamax (Hyclone ...
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bioRxiv - Molecular Biology 2023Quote: ... at 55 cm Hg for 1.5 hrs with RNA transfer buffer (3 M NaCl, 0.01 N NaOH) using a VacuGene XL (GE Healthcare). The membrane was rinsed with 2x SSC for 10 min ...
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bioRxiv - Cell Biology 2024Quote: Col1a1-H2B-GFP;Rosa26-M2-rtTA;Slc23a2FL/FL;Mx1-Cre mice and Col1a1-H2B-GFP;Rosa26-M2-rtTA;Slc23a2FL/FL littermate controls were given 3 intraperitoneal injections of 40µg of poly I:C (GE Healthcare) dissolved in PBS over 5 days to induce Cre expression ...
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bioRxiv - Bioengineering 2023Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
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bioRxiv - Plant Biology 2023Quote: ... Dried eluates were dissolved in 4 M urea with 0.1% ampholytes (pH 3–10; GE Healthcare, Chicago, IL, USA) and fractionated by off-gel focusing ...
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bioRxiv - Neuroscience 2023Quote: Both structural and functional MRI data were obtained using a 3-T scanner (Discovery 750; GE Healthcare, Milwaukee, WI). High-resolution T1-weighted structural images were captured using a three-dimensional brain-volume sequence (TE = 3.18 ms ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 3 days post transfection and purified using GraviTrap TALON columns (GE Healthcare # GE29-0005-94). The columns were first equilibrated with purification buffer (50 mM sodium phosphate pH 7.4 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 3 h with HRP-streptavidin in TBS/10% FBS (1:200; GE Healthcare Life Sciences). After a final wash in TBS (3 x 10 min) ...
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bioRxiv - Synthetic Biology 2024Quote: ... with HBS-EP+ as the running buffer (10 mM HEPES at pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Proteins were immobilized on a CM5 chip (GE Healthcare #29104988 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein A/G Sepharose beads (17-5280-01, 17-0618-01 - GE Healthcare (3:1 ratio A/G beads)) were added for 2 hours to precipitate antibody bound complexes ...
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bioRxiv - Biochemistry 2024Quote: ... that recognizes amino acids 3-8 of Aβ and the Protein G Sepharose 4 Fast Flow resin (GE healthcare) (Tables 2 and 3 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with 1-3 nM non-targeting siRNA as a control (D-001810-10-05, GE Healthcare Dharmacon) or with siRNAs targeting PDK2 and PYCR1 (Dharmacon ...
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bioRxiv - Bioengineering 2020Quote: ... and 35 ml of this suspension was carefully added to 15 ml Ficol-Paque PLUS (1.077 g cm-3, GE Healthcare) and centrifuged at RT ...
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bioRxiv - Neuroscience 2020Quote: ... washed three times with 0.1 M cacodylate and then incubated for 3-h at RT in streptavidin HRP conjugate (1:200; GE Healthcare). Sections were visualized after 1-min and 30-sec DAB (Sigma-Aldrich)/glucose oxidase reaction ...
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bioRxiv - Plant Biology 2021Quote: ... and the peptides used for SPR was dissolved in HBS-EP+ (10 mM HEPES, 150 mM NaCl, 3 mM EDTA and 0.05% [v/v] surfactant P20 (GE Healthcare)) ...
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bioRxiv - Immunology 2020Quote: ... IgGs were purified from ∼1-3 ml of serum using equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted off the resin with 0.1 M glycine pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was centrifuged for 10 minutes at 25,000g and the lysate supernatant was incubated for 3 hours with 1 mL of glutathione sepharose beads (GE Healthcare) pre-washed in lysis buffer ...
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bioRxiv - Biophysics 2020Quote: ... A volume of 50 μl of each sample at 3 mg·ml−1 was loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 25 mM Hepes pH 7.8 ...
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bioRxiv - Immunology 2020Quote: ... Then cells were washed 3 times with PBS and stained with 75 μM CypHer5E NHS ester (GE Healthcare, PA #15401) for 1 hour at 25°C[13] ...
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bioRxiv - Biochemistry 2021Quote: ... anti-EGFR antibody (#MI-12-1, MBL), anti-GFP antibody (#M-048-3, MBL) and Protein A-Sepharose (GE Healthcare) were used for immunoprecipitation ...
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bioRxiv - Immunology 2020Quote: ... with a 3 kDa membrane cutoff and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare Life Sciences) size exclusion chromatography column previously equilibrated with 7M urea ...
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bioRxiv - Physiology 2022Quote: ... Membranes were then washed 3 more times and the signal was detected by chemiluminescence using ECL Prime reagent (GE Healthcare) on an Amersham Imager 600 detection system (GE Healthcare ...