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Citations for GE Life Sciences :
101 - 150 of 1867 citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... were acquired every 10 or 15 minutes for a 24-36 hr period with a 40x oil-immersion 1.3 NA objective using an Olympus DeltaVision Elite microscope (Applied Precision, LLC) equipped with a CoolSNAP HQ2 camera (Roper Scientific) ...
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bioRxiv - Microbiology 2020Quote: dHepaRG or PHH cells seeded into a 24-well plate were transfected with 25 nM or 10 nM of siRNAusing Dharmafect#1 (GE HealthCare) or Lipofectamine RNAiMax (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... The membrane was incubated first with primary antibodies for 24 h at 4°C and then with horseradish peroxidase– conjugated secondary antibodies (GE Healthcare) for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood within 24 hours of sample collection using Ficoll-Paque (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... GST-FliJ(Δ13–24) and GST-FlgN were purified by GST affinity chromatography using a glutathione Sepharose 4B column (GE Healthcare) as described previously28 ...
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bioRxiv - Immunology 2020Quote: ... FP3 or FP4 from the Mono S column were pooled and further purified by SEC performed on a 24 mL Superdex 200 increase column (GE Healthcare) at 4 °C with a flow rate of 0.25 mL/min in a buffer containing 20 mM HEPES ...
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bioRxiv - Physiology 2020Quote: ... Blots were then washed in TBST and incubated for 1 h at 22-24°C with secondary HRP-conjugated antibodies (GE Healthcare) diluted 5000-fold in 5% skim milk in TBST ...
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bioRxiv - Cell Biology 2020Quote: ... Micrographs in time frames of 10 min were acquired during 24 h in a Deltavision RT imaging system (Olympus IX70/71, Applied Precision) equipped with a Plan apochromatic 20X/1.42 N.A ...
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bioRxiv - Biochemistry 2021Quote: The eluent from the Ni-NTA purification of the expression product of the pET-Duet-1 vector in BL21(DE3)pLysS* cells was loaded onto a 24 ml Superdex-200 10/300 GL (GE Healthcare) column on an AKTA Purifier-10 workstation ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was concentrated and subjected to size exclusion chromatography on Superdex 200 increase semipreparative column (10/300, bead volume: 24 ml; GE Healthcare), also equilibrated with WB ...
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bioRxiv - Microbiology 2020Quote: ... The quality of the amphipol associated complex was controlled by performing an additional size exclusion step on a Superdex 200 increase column (10/300, bead volume: 24 ml; GE Healthcare).
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bioRxiv - Biophysics 2023Quote: Proof-of-concept data were acquired in six healthy volunteers (five female, mean age 30 years, range 24 – 46 years) on a simultaneous 3T SIGNA PET-MR scanner (GE Healthcare); ethics approval was granted by the University of Manchester Research Ethics Committee (reference ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were obtained 24 hours after treatment and GST was purified on a 1 mL GSTrap HP column (GE Healthcare) with an AKTA purifier 10 FPLC system ...
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bioRxiv - Biophysics 2022Quote: ... This was followed by size exclusion chromatography (SEC) using 24 ml Superdex 200 Increase 10/300 GL column on an AKTA-FPLC system (GE Healthcare). Analytical gel filtration was performed in 20 mM Tris pH 7.5 and 150 mM NaCl at the flow rate of 0.5 ml/min.
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bioRxiv - Molecular Biology 2022Quote: ... The blots were then exposed onto autoradiography films for 24-48 hours and the signals were detected with the phosphorimager (Fuji, FLA-7000, GE Healthcare). The signal intensities of the images were measured using ImageJ.
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bioRxiv - Plant Biology 2022Quote: ... oryzae isolates were maintained on Potato Dextrose Agar medium at 24°C and as frozen stocks of dried mycelium on Whatman filter paper (GE Healthcare Whatman™ Qualitative Filter Paper ...
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bioRxiv - Biochemistry 2023Quote: GB1-FKBP-ICD and GB1-FRB-ICD were mixed at equimolar ratio and loaded onto a 24-mL Superdex 200 column (GE healthcare) equilibrated in 100 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated first with primary Abs for 24 hours at 4°C and then with HRP–conjugated secondary Abs (GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Concentrated eluates were then applied to a 24-mL Superdex S200 increase 10/300 GL size exclusion column (GE Life Sciences). Fractions containing Cdc13 (Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were collected 24 hours post-transfection and the RNA was isolated using the Illustra RNAspin Mini RNA Isolation Kit (GE Healthcare). The pattern of alternative splicing of individual minigenes was detected by RT-PCR assays ...
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bioRxiv - Neuroscience 2024Quote: ... The baseline imaging used for prediction consisted of MRI performed between 24 and 72 hours after stroke onset on a 3T scanner (Discovery MR750w, GE Healthcare). We provided the raw Diffusion-Weighted Images (DWI ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were developed using SuperSignal West Femto Chemiluminescent Substrate (34095; Pierce) and SuperSignal West Pico PLUS Chemiluminescent Substrate (34078; Pierce) on Hyperfilm ECL (18 × 24; GE28-9068-37; GE Healthcare) in a Curix 60 (AGFA ...
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bioRxiv - Cell Biology 2020Quote: siRNA-mediated knockdown in 786-O cells was carried out using 5-10 nM Dharmacon ON-TARGETplus siRNAs (GE Healthcare), diluted in siRNA buffer and mixed with DharmaFECT transfection reagent (1:50 in optiMEM reduced serum media) ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was filtrated through a O.22μm pore filter and loaded on a 5 ml pre-packed Hitrap Q FF column (GE Healthcare) equilibrated with buffer A (50mM bis-tris-HCI pH 6.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
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bioRxiv - Immunology 2022Quote: ... and resuspended in 24 mL of RT 70% PercollTM (1.088 g/mL at 22 °C; GE Healthcare Life Sciences 17-0891-01). Aliquots of 8 mL cell/PercollTM suspension were overlayed with 4 mL HBSS and centrifuged at 400 xg for 30 minutes RT ...
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bioRxiv - Neuroscience 2020Quote: ... BV2 cells were seeded in 24 or 6 well plates 24 hours prior to the transfection assay in high glucose DMEM supplemented with 10% fetal bovine serum (FBS, GE Healthcare Hyclone) and 1% antibiotic-antimycotic (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... human ECs were transfected twice (at 0 and 24 h) with 200 pmol of siGENOME Non-Targeting siRNA #1 (as control) or siGENOME SMART pools siRNA oligonucleotides (GE Healthcare Dharmacon), using Oligofectamine Transfection Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were further purified using a HiLoad 16/600 24 ml Analytical Superdex™ 200 Increase pg gel-filtration column (GE Healthcare) with 20 mM Tris ...
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bioRxiv - Bioengineering 2024Quote: ... The printed structures were then incorporated into a micro physiological device in order to evaluate the oxygen concentration in printed structures.[68] The oxygen concentration in the printed structures was estimated using a fluorometric method by imaging the entire device at regular intervals for a period of 24 hours using Incell Analyser 6000 (GE Life Sciences) and measuring the intensities of the beads ...
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bioRxiv - Developmental Biology 2021Quote: ... 4-16-cell embryos were transferred to 3 % Ficoll (GE Healthcare) in 0.6x MMR and injected with 5-10 nl of a solution containing mRNAs and/or MOs ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% (v/v) ampholytes (pH 3-11 NL; GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 3 hours with His beads (GE Healthcare) prewashed in His binding buffer ...
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bioRxiv - Immunology 2020Quote: ... cDNA constructs were inserted into the pGEX6P-3 plasmid (GE Healthcare) and expressed as GST-fusion proteins in High efficiency BL21(DE3 ...
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bioRxiv - Immunology 2021Quote: ... 3 or 4 of a Series S CM5 chip (GE Healthcare) functionalised with an anti-His capture kit (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: Early passage HUVECs were coated on Cytodex-3 beads (GE Healthcare) at a density of 10 million cells/40 μl beads and incubated in suspension for 3-4 hours with gentle mixing every hour ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleavage products were separated by gel electrophoresis on 15% denaturing polyacrylamide gel containing 7.5M urea and 24% formamide and visualized using a Typhoon 9400 Scanner (GE Healthcare Chicago, IL, USA). Activity assays performed with purified Argonaute proteins were carried out with 17 nt long guide G-2 (DNA ...
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bioRxiv - Plant Biology 2021Quote: ... Radioactive gels were exposed to a BAS phosphor-imaging plate (TR2040, Fuji) for 24 h and visualised using a Typhoon scanner (GE Healthcare, FLA-9500).
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bioRxiv - Immunology 2020Quote: ... and 0.1 M 3-(N,N-dimethylamino) propyl-N-ethylcarbonadiimide (GE healthcare) at a flow rate of 5 µL/min ...
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bioRxiv - Microbiology 2021Quote: ... dadantii OutB was cloned into the pGEX-6P-3 vector (GE Healthcare) encoding a cleavable N-terminal Glutathione S-transferase (GST ...
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bioRxiv - Molecular Biology 2024Quote: ... using a multi-frequency linear array transducer (3-12 MHz, GE Healthcare). Participants were instructed to lay on an athletic training table in the supine position 5 minutes prior to imaging ...
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bioRxiv - Cell Biology 2024Quote: ... 3D-SIM was carried out using an OMX Version 3 (Applied Precision) microscope equipped with a UPlan APO 100× 1.4 NA oil-immersion objective (Olympus) ...
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bioRxiv - Biochemistry 2022Quote: ... and 3 mM MgCl2] using a size exclusion column (NAP5; GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... blotted for 3 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Size-exclusion chromatography was performed on a 24 ml Superdex-75 gel filtration column on an ÄKTA Purifier System (GE Healthcare, Chicago, Illinois, USA) using the same buffer as during sedimentation velocity measurement ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were imaged for GFP and mCherry 24 h after initial plating (t=0) and at the indicated timepoints using a 4X objective InCell Analyzer system (GE Healthcare Life Sciences, Marlborough). Segmentation and counting of GFP- and mCherry-positive cells were performed using an Acapella script (PerkinElmer ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were exposed to a phosphoscreen for 24 h that was scanned on a phosphoimager (Typhoon FLA 9500, GE Healthcare, Chalfont ST Giles, UK). Primers and oligonucleotides used for probe synthesis are listed in Supplemental Table 2.
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3 mM TCEP and subsequently concentrated (Vivaspin 20 3 kDa MWCO, GE Healthcare) to ~10 mg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... or pH 3–10 for CDK2 (Amersham Biosciences, GE Healthcare Europe, Diegem, Belgium) before separation by SDS-PAGE (42 ...