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Citations for GE Life Sciences :
2351 - 2400 of 4168 citations for 7 Oxa 1 thia 3 azaspiro 4.4 non 2 ene 4 6 dione 2 amino 8 8 dimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... was used in the experiments shown in figure 3 and supplementary figure 6C while a pool of four scrambled siRNAs (#D-001206-13-05, GE Healthcare Dharmacon) was used in the experiments shown in figure 2 ...
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bioRxiv - Cancer Biology 2020Quote: DNA probes complementary to the TP53 coding region or the 3′UTR were labeled with dCTP [α-32P] using the Amersham Megaprime DNA labeling system (GE Healthcare). Primers used for probe synthesis from human cDNA are listed in Supplementary Table 1 ...
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bioRxiv - Neuroscience 2020Quote: The blots were subsequently washed 3 x 5 min in TBST 1X and developed with the Prime Western Blotting detection Reagent (GE Healthcare Amersham) using the LAS3000 Bioimager (Fujifilm ...
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bioRxiv - Cancer Biology 2019Quote: ... was added to sample tubes and allowed to sit at room temperature for 3 minutes before quenching with 1.0 mL DMEM (GE Life Sciences, SH30081.01) + 6% FBS (Omega Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... induced for 3 h induction by IPTG (isopropyl b-D-thiogalactopyranoside) and soluble extracts were purified using GST-sepharose 4B (GE Healthcare, Sweden) as described elsewhere (Geisler et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were then washed again for 3 × 15 min in PBS-T and signals were detected with ECL Western Blotting Detection Reagent (GE Healthcare, #RPN2106) and acquired with Chemidoc MP Imaging System (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were isolated by diluting whole blood sample 3-fold with RPMI 1640 medium (Biological Industries Israel Beit-Haemek) and loading the material on Ficoll-Paque PLUS (GE Life Sciences) at a ratio of 1.8 ...
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bioRxiv - Pathology 2021Quote: ... The culture medium containing pseudotyped particles was collected after 48 hr and concentrated 3 times using Vivaspin columns with 100 kDa cutoff (GE Healthcare, #28932363) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Before revelation membranes were washed again 3 times in TBS-T for 5min and then incubated in ECL™ Western Blotting Detection Reagent (RPN2209, GE Healthcare) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The next day the protein was dialyzed for an additional 3 hours in fresh storage buffer and subsequently applied to a Superdex 200 10/300 GL (GE Life Sciences) equilibrated in the same buffer ...
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bioRxiv - Cell Biology 2022Quote: ... for 2.5 hours at 37 °C and 13mm coverslips were silanized for 3 minutes using ethanol containing 0.37% Bind-Silane solution (GE Healthcare Life Science) and 3.2% acetic acid ...
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bioRxiv - Cell Biology 2023Quote: ... The eluate fraction was dried using a vacuum centrifuge and resuspended in 3.6 mL ampholyte solution (pI 3-10, GE Healthcare, Chicago, IL). Offgel fractionation was performed using a 3100 OFFGEL fractionator (Agilent ...
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bioRxiv - Bioengineering 2023Quote: ... slice thicknesses of 1.5 and 3 mm) with or without intravenous iodixanol contrast medium (320 mg/mL; GE Healthcare, Little Chalfont, UK) and evaluated for IVCF radiopacity ...
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bioRxiv - Molecular Biology 2023Quote: ... the NbaAgo samples (3 mg/ml in 33 μl) were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare, USA) operated on a ProStar335 system and connected to a tandem of Prostar 335 detector (Varian ...
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bioRxiv - Microbiology 2023Quote: Codon-adapted genes (see above) of vhemO and vpcyA/X from the CB_2 phage were cloned in pGEX-6P-3 (GE Healthcare Life Sciences), resulting in a tac promoter-driven expression of a glutathione-S-transferase fusion protein with the protein of interest ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant obtained by centrifugation (19,000 × g, 15 min, 4°C) was applied to a His SpinTrap column (GE Healthcare) previously equilibrated with buffer A ...
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bioRxiv - Bioengineering 2020Quote: ... 10% glycerol (v/v) at 4°C and re-loaded onto a Ni-NTA column (HisTrap™ HP, GE Healthcare). An imidazole gradient was applied (from 20mM to 500mM ...
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bioRxiv - Cell Biology 2020Quote: Fresh embryo protein extracts (4 mg in 2.5 ml RIPA buffer) was passed through PD-10 desalting column (GE Healthcare) to remove excess free biotin using the gravity protocol according to manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was recovered by centrifugation (50,000 g, 20 min, 4°C) and applied to a HisTrap FF NiNTA column (GE Healthcare). After washing with buffer A (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... images were obtained every 5 min for 4 h using a 20X objective (0.75 NA, PlanApo) on a DeltaVision microscope (GE Healthcare) equipped with a CoolSNAP HQCCD camera (Photometrics ...
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bioRxiv - Cell Biology 2020Quote: ... diluted with 4 mL 0.5% Triton X-100 in PBS (PBS-T) and incubated with prepared glutathione sepharose beads (GE Healthcare) in a gravity column for 3 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 5% glycerol (w/v, 0.1% Triton X-100) and mixed with 50 µl Glutathione Sepharose 4 Fast Flow (GE Healthcare) slurry ...
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bioRxiv - Molecular Biology 2020Quote: ... was pre-equilibrated for 1.5 hrs at 4°C with Sup6-150 buffer without inhibitors or DTT on an AKTA Purifier system (GE Healthcare). 500 μL lysate (2 mg protein ...
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bioRxiv - Cell Biology 2019Quote: ... ultra-centrifuged (280.000 x g, 10 min, 4°C) and gel-filtered using a Superose6 10/300 GL column (GE Healthcare). Peak fractions were pooled ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C) and the supernatant was applied to a 5ml HiTrap IgG Sepharose FF column (GE Healthcare, #28-9083-66). After washing with APC purification buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Sonicated lysate was cleared by centrifugation at 12,000g for 20 minutes at 4°C and then applied to pre-washed glutathione sepharose beads (GE healthcare) overnight at 4°C with rotation ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 48,284 g for 45 min at 4°C and loaded into a HisTrap FF cartridge (GE Healthcare). Proteins were eluted with 100 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The solution was dialyzed against Buffer C overnight at 4 °C and applied on a Resource Q column (GE Healthcare) that was equilibrated with Buffer C ...
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bioRxiv - Biochemistry 2020Quote: ... and 500 μL (4 μg BSA) was injected into an AKTA™ PureL system (GE Healthcare Life Sciences, Chicago IL), and run over a Superdex 200 GL (10/300 ...
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bioRxiv - Biochemistry 2020Quote: ... After the reaction, the sample was centrifuged briefly (10 min, 16,000g, 4°C) and loaded onto a Superdex 200 column (GE Healthcare) to remove Sfp and unreacted dsOligo-CoA ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was centrifuged for 30 min at 30.000x g at 4 °C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged proteins were eluted with a linear gradient of buffer B (50 mmol L−1 Hepes at pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin was then precleared overnight at 4°C with vertical rotation at 15rpm (a total of 5 reactions per condition in 15ml tubes) with 500µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) beads and then used for immunoprecipitation.
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bioRxiv - Molecular Biology 2021Quote: ... and immunoprecipitated again in a single reaction with 100µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) pre-conjugated with histone H3 and histone H2B antibodies in order to specifically enrich for chromatin-associated dCas9 complexes and eliminate from the purification unspecific non-chromatin associated dCas9 complexes ...
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bioRxiv - Microbiology 2021Quote: ... The solution was centrifuged for 30 min at 38,000 × g and 4°C to remove precipitates and applied onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB3 ...
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bioRxiv - Microbiology 2020Quote: ... and the others were incubated with the indicated antibodies overnight at 4°C.Then mixed with the Protein G sepharose beads (GE Healthcare) for 2 h at 4°C.The immunoprecipitates were washed four to six times with the 293T lyses buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared by two rounds of 20 min centrifugation at 45,000 x g at 4°C and loaded onto a 5-mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... Insoluble material was sedimented by centrifugation at 13,500 g for 30 min at 4°C prior to loading on His-trap FF columns (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... filter and size-exclusion chromatography at 4 °C on a Superdex 200 10/300 GL gel filtration column (GE Healthcare). Purity of the proteins was evaluated on SDS-PAGE gel ...
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bioRxiv - Pathology 2021Quote: ... the crude protein solution was finally collected by ultracentrifugation (40,000 rpm, 30 min, 4°C) and subsequently incubated with Ni-NTA (GE healthcare) resin for 2 h at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was added to 50 µl G-Protein Sepharose beads (Protein G-Sepharose 4 Fast Flow, GE Healthcare) (pre-washed with the lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... labelled protein samples were subjected to size exclusion chromatography at 4°C (Superdex 200 Increase 10/300 GL, GE Healthcare) in 50 mM phosphate (pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cooled down on ice and ultracentrifugated at 160,000xg for 1hr at 4°C and loaded onto a 5 mL Protein A (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Immunology 2022Quote: ... Polyclonal IgG from subject 391370 was isolated from heat-inactivated plasma using Protein G Sepharose 4 Fast Flow (GE Healthcare). Purified IgG was buffer exchanged into Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was dialyzed against 20 mM Tris-HCl pH 8.0, centrifuged (15 min, 48000xg, 4 °C) and loaded onto a 15 ml Q-sepharose FF (GE Healthcare) column equilibrated with the same buffer ...
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bioRxiv - Microbiology 2019Quote: ... 5-10 µg of proteins were resolved on 4–12% SDS-polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with various antibodies prior to development with ECL prime (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... the concentrated protein was gel-filtrated using a Superdex 200 10/300 GL column equilibrated in HBS at a flow rate of 0.5 ml/min and 4 °C (GE Healthcare). Peak fractions at a final OD280nm of ∼ 0.4 (∼ 1.4 μM ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... and then the eluate was incubated overnight on a rotator at 4°C.The protein was resolved on a HiLoad 16/600 Superdex 200 Column (GE Healthcare) pre-equilibrated with storage buffer (20 mM HEPES-KOH ...
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bioRxiv - Molecular Biology 2021Quote: ... the soluble proteins were collected after a 5 min centrifuge at 17,000 g at 4 ℃ and immobilised on Glutathione-coated sepharose beads (GE Healthcare). For pull down with 35S-radiolabelled preoteins ...