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Citations for GE Life Sciences :
2301 - 2350 of 4168 citations for 7 Oxa 1 thia 3 azaspiro 4.4 non 2 ene 4 6 dione 2 amino 8 8 dimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: A total of 1.5 mg each of 0 hr and 3 or 5 hr ssDNA samples were labeled with Cy3-dUTP or Cy5-dUTP (GE Healthcare) by random priming without denaturation with 4 mg random nonamer oligo (Integrated DNA Technologies ...
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bioRxiv - Cell Biology 2020Quote: Computerized axial tomography scanning (CT scan) was performed on anesthetized P30 mice [3% of Isoflurane (Isoba Vet)] using an eXplore ista PET-CTscan (GE Healthcare) with the following parameters ...
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bioRxiv - Cell Biology 2020Quote: ... antigen was CENP-AHCP-3(105-183)::6xHis) and detected using an HRP-conjugated secondary antibody (rabbit or mouse; GE Healthcare). For antibody production CENP-AHCP-3(105-183)::6xHis was expressed in E ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 mg of 3C protease per 3 L of culture volume and further separated on a Superdex 200 HiLoad column (GE Healthcare) in 50mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM NaCl) at approximately 3 mg/mL and then further purified by size-exclusion chromatography using a Superdex-200 column (GE Healthcare) equilibrated in solubilization buffer ...
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bioRxiv - Cell Biology 2020Quote: ... A probe was generated from a PCR fragment homologous to the 3’ sequence just outside of the targeted region using the AlkPhos direct labelling kit (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the pirA gene was cloned into XhoI and EcoRI sites of pGEX-4T-3 vector (GE Healthcare Life Sciences, Freiburg, Germany), encoding recombinant PirA with N-terminal-fused GST-tag ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was incubated for 3 h at room temperature and loaded onto a Superdex 200 10/300 GL (GE Healthcare) sizeexclusion chromatography column using buffer B as mobile phase ...
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bioRxiv - Genomics 2022Quote: ... Samples were then loaded onto a 1% agarose gel containing 3% formaldehyde and 1x MOPS and run at 75 V in 1x MOPS for ~3 hs after which RNA was transferred onto a Hybond N+ membrane (GE Healthcare) overnight ...
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bioRxiv - Synthetic Biology 2022Quote: ... then wrapped into a clear foil and exposed in the cassette with the phosphor screen for 3 days and visualized on a Phosphorimager (Typhoon FLA 7000, GE Healthcare). prDC1416 was used to generate the blot in Fig ...
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bioRxiv - Microbiology 2024Quote: ... The eluted Cas1-Cas2/3 complex was concentrated and further purified by gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer C ...
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bioRxiv - Biophysics 2024Quote: ... His6-GB1-tag was subsequently cleaved by TEV protease (3 mM) and separated from bZIP on a cation exchange column (HiTrap SP; GE Healthcare) using a 0–2 M gradient of sodium chloride ...
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bioRxiv - Microbiology 2023Quote: ... The eluted fractions were concentrated to 3 ml and applied to 120 ml HiLoad 16/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... control HUVEC or HUVEC 48 hr post-siRNA treatment were incubated with cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) overnight then resuspended in 2.2 mg/ml fibrinogen (820224 ...
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bioRxiv - Neuroscience 2024Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 or Amicon 15 centrifugal concentrators (10 kDa, 5 kDa, or 3 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Neuroscience 2024Quote: All scans were conducted in a 3 Tesla General Electric scanner with an eight-channel head coil (GE MR750; GE Healthcare). Anatomical images were acquired via T1-weighted ...
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bioRxiv - Cell Biology 2024Quote: ... Parasites were synchronized to a 3-h age window by isolating late schizonts from a 60% Percoll (GE Healthcare, Life Sciences) gradient and culturing these for 3 h with fresh erythrocytes (104) ...
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bioRxiv - Cell Biology 2024Quote: All TIRF experiments (except Figure 3 F and G) were acquired at 37 °C/5% CO2 with the DeltaVision OMX SR microscope (GE Healthcare) with a 60×/1.42 NA oil Plan Apochromat objective (Olympus ...
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bioRxiv - Developmental Biology 2024Quote: ... a cDNA fragment encoding the C-terminal portion of mouse Ccrk (residues 289-346) was amplified by PCR and subcloned into the pGEX-4T-3 vector (GE Healthcare). The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3 ...
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bioRxiv - Cell Biology 2019Quote: ... Lysate was cleared with centrifugation and bound to Glutathione sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-02). Beads were washed several times with lysis buffer and protein was eluted from beads with 10 mM reduced glutathione in lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... All protein lysates were run on NuPage 4–12% gradient gels (LifeTechnology) and transferred to PVDF membrane (GE Healthcare). Membranes were blocked in 5% milk in TBST and stained with primary antibody overnight at +4°C ...
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bioRxiv - Microbiology 2022Quote: ... as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... C (W6/32) and rotated with 30 μl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 2 hours at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... overnight at 4°C then purified over a Sephadex 200 Increase 10/300 (GE Healthcare Life Sciences, Pittsburgh, PA) size exclusion column in Buffer A with 0.02% DDM.
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bioRxiv - Biochemistry 2021Quote: ... The solution was added to 250 μL (bed volume) Protein G Sepharose™ 4 Fast Flow resin (GE Healthcare) prewashed with DPBS ...
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bioRxiv - Microbiology 2022Quote: ... and for HLA-I IP 25 μl Protein G Sepharose® 4 Fast Flow (GE Healthcare, GE17-0618-01) were employed with the HC10 antibody for HLA-IP overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... The concentrated CM was loaded onto a column packed with Sepharose 4 Fast Flow resin (GE Healthcare, IL, USA). Eluates were measured at 280 nm absorbance to monitor the protein content of each fraction ...
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bioRxiv - Biochemistry 2019Quote: RAD51 purification was carried out by chromatography at 4°C using the AKTA Pure Protein Purification System (GE Healthcare). For WT RAD51 ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified GST and GSTPib1ΔFYVE (100 pmol) were immobilised on glutathione Sepharose 4 Fast Flow (25 μl slurry, GE Healthcare). Beads were pre-treated with 1 mg/ml bovine serum albumin (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... pH 4) was covalently immobilized on a CM5 sensor chip using an Amine coupling kit (GE Healthcare Life Sciences). Binding analyses were performed in HBS-P buffer (0.01 M HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1h at 4°C followed by incubation with protein A-sepharose beads (GE Healthcare #GE17-0780-01) for 2h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission, GE Healthcare). Fusion protease was removed using glutathione resin and the cleaved β2HA was collected in the flow-through ...
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bioRxiv - Microbiology 2020Quote: ... Sgo_R10 and Sgo_R3-4 were further purified by preparative SEC using a Superdex 75 16/600 column (GE Healthcare) equilibrated in 20 mM Tris.HCl ...
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bioRxiv - Immunology 2020Quote: ... culture media was collected and incubated overnight at 4°C with nickel-Sepharose Excel (GE Healthcare 17-3712-01). His-tagged proteins were eluted from the nickel-Sepharose with 1 M imidazole in TrisCl pH 8.5 ...
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bioRxiv - Biochemistry 2022Quote: All chromatography steps were performed at 4 °C using the ÄKTAxpress and ÄKTA pure protein purification systems (GE Healthcare). For affinity purification of the ADGRG4 construct ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Plant Biology 2024Quote: Analytical SEC experiments were carried out at 4 °C using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in SEC running buffer ...
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bioRxiv - Biochemistry 2024Quote: ... All purification steps were performed at 4°C and the pre-packed amylose column (5ml Hi-Trap GE healthcare) was equilibrated with 25 mL Amylose column A Buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining purification steps were carried out at 4 °C using an ÄKTA protein purification system (GE Healthcare/Cytiva) for column chromatography steps ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Biochemistry 2023Quote: ... and ∼ 4 mL of this concentrated ABHD14B mutant was loaded onto a pre-equilibrated HiLoadTM16/60 Superdex200 (GE Healthcare) gel filtration column ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE using 4% or 5% polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with mouse monoclonal antibody generated against HA-tag peptide (YPYDVPDYA ...
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bioRxiv - Bioengineering 2024Quote: ... 10% glycerol (v/v) at 4°C and re-loaded onto a Ni-NTA column (HisTrapTM HP, GE Healthcare). An imidazole gradient was applied (from 20mM to 500mM ...
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bioRxiv - Biophysics 2024Quote: ... Lysates containing fascin were loaded onto a column packed with glutathione Sepharose 4 FF resin (GE Healthcare, Chicago, IL). Fascin recovered from the glutathione column was cleaved with Prescission protease overnight at 4º C ...
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bioRxiv - Cancer Biology 2024Quote: ... supernatants were incubated with 30 µL of protein G Sepharose™ 4 Fast Flow (#17-0618-01, GE Healthcare) under agitation for 4 hours at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The complex was resolved by gel filtration at 4 °C with a Superdex 200 10/300 column (GE Healthcare) equilibrated with 75 mM KCl ...
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bioRxiv - Neuroscience 2019Quote: ... Each probe contains a 3’-end amine-modified nucleotide that allows directly couple to an NHS-ester Cy3 dye (GE Healthcare, PA23001) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Postnuclear supernatants were incubated with 3 μg of antibodies coupled to protein A– or protein G–Sepharose beads (GE Healthcare, Solingen, Germany) for 4 h at 4°C ...