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Citations for GE Life Sciences :
2251 - 2300 of 2314 citations for 8 Chloro 2 chloromethyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... was used at 1:5000.) Blots were incubated with chemiluminescent detection reagent (Amersham ECL™ Prime Western Blotting Detection Reagents, GE Healthcare #RPN2236) and imaged on an Amersham Imager 600 (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... nitrocellulose membranes were blocked in 3 % BSA in TBST and stained with HRP-conjugated streptavidin (GE Healthcare Life Science, Cat# RPN1231vs,1:2500) in blocking solution ...
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Rhodococcus equi Escapes Macrophage Autophagy: VapA Inhibits ATP6V0d1-Mediated Lysosomal DegradationbioRxiv - Immunology 2023Quote: ... second antibody (1:10000) for 1 h at room temperature and then proteins were visualized with enhance chemiluminescence (ECL) kit purchased from GE Healthcare (Chicago, USA). And detection reagent and analyzed using GE Amersham Imager 600.
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bioRxiv - Bioengineering 2023Quote: ... Released mesh probes were rinsed with deionized (DI) water three times and transferred to sterile 1× PBS solution (HyClone, GE Healthcare Life Sciences) before surface modification or implantation.
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bioRxiv - Biochemistry 2024Quote: ... H2AX (total) (#2595S; Cell Signaling technology]. The membranes were extensively washed with 1% TBST followed by incubation with anti-isotype secondary antibody (GE Healthcare) conjugated with horseradish peroxidase in 5% skimmed milk at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Digested samples were passed through pre-washed (4x with 700 μl polysome buffer for 1 min at 450 x g) Sephacryl S-400 HR spin columns (GE healthcare, currently Cytiva), and centrifuged for 2 min at 450 x g at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... The fusion protein was isolated from soluble cell lysate by Co-NTA affinity chromatography with two His-Trap 1 mL TALON Crude columns (GE Healthcare Life Sciences, 28953766). Chromatography buffers contained sodium phosphate 20 mM pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Whole genome DNA was amplified using 1 μl polymerase enzyme from the illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Cat# 25-6600-32) and 9 μl of sample with the following conditions ...
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bioRxiv - Immunology 2021Quote: ... The Fc and IdeS A were removed using a mix of Protein A Sepharose® Fast Flow (250 µL per 1 mg digested mAb; GE Healthcare Life Sciences) and Ni Sepharose™ 6 Fast Flow (50 µL per 1 mg digested mAb ...
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bioRxiv - Microbiology 2019Quote: ... The filtrate was diluted to 500 µl with running buffer A (50 mM Tris, pH 9) and loaded on a 1 ml Resource™ Q anion exchange column (GE Healthcare Life Sciences). A linear gradient to 40% running buffer B (50 mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was then incubated with primary antibody diluted in TBST/BSA for one hour followed by a one hour incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:10,000, GE Healthcare life sciences, Piscataway, NJ). Primary antibodies used were ...
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bioRxiv - Molecular Biology 2019Quote: ... fused to triple epitope tags were prepared by directionally cloning in-frame epitope tags into the BamHI and EcoRI restriction sites of pGEX-4T-1 (GE Healthcare Life Sciences #28954549). PCR amplicons were prepared from a PAGE-purified oligonucleotide template with forward primers containing a BglII restriction site and reverse primers containing HindIII–BamHI–EcoRI sites (Supplementary Table S1) ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mL fractions corresponding to ∼10 mL of the elution peak were pooled and applied to a 1 mL StrepTrap column (GE Healthcare, #28-9075-46) pre-equilibrated with Buffer N with 2 mM DTT ...
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bioRxiv - Plant Biology 2020Quote: ... A peroxidase-conjugated secondary antibody was used at a dilution of 1:200 and immunoreactive bands were visualized with ECL Western Blotting Detection Reagents (GE Healthcare, USA; https://www.gelifesciences.com). Rubisco protein content was quantified from SDS-page gels using ALPHA-Ease FC software (Alpha Innotech ...
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bioRxiv - Biochemistry 2021Quote: Three microliters of S-D614G in 1.2 mg/ml was mixed with 1.2 equivalent of RBD-chAb45 and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mm × 1 mm × 1 mm) and brain FDG-PET were acquired simultaneously on a hybrid 3 T SIGNA PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the CUB Hôpital Erasme (Brussels ...
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bioRxiv - Plant Biology 2020Quote: ... Fifty seeds per replicate were sown in Petri dishes containing three layers of filter paper (Whatman grade 1, GE healthcare, Little Chalfont, United Kingdom) and imbibed with 4 mL of ultrapure water (UPW) ...
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bioRxiv - Neuroscience 2020Quote: ... installed prone on the MRI bed and anaesthesia was immediately switched to 1% ISO in medical air through a respirator (Aestiva, GE Healthcare, Datex-Ohmeda, USA). The respirator allowed continuous control of respiration rates ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified using a NiNTA purification system (HisTrap™ excel 1 mL) and size-exclusion chromatography using HiPrep™ 26/60 Sephacryl™ S-100 HR (GE Healthcare) in 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: A paper punch was used to make 3 mm diameter circles of Whatman™ Grade 1 180 μm thick cellulose filter paper (GE Healthcare Life Sciences), which were stored in sterile disposable petri dishes ...
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bioRxiv - Cancer Biology 2019Quote: ... for 1 h incubated with the appropriate antibodies and then visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Cell Biology 2019Quote: ... and Gabarapl2 (EcoRI/SalI) were amplified from cDNA derived from B16F10 melanoma cells and cloned in the vector pGEX-4T-1 (GE Healthcare, Chicago, Illinois, USA) into the sites indicated in parentheses ...
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bioRxiv - Synthetic Biology 2019Quote: ... between 5 and 10 mg protein ml−1) was pipetted into the measurement cell of a MicroCal ITC200 isothermal titration calorimetry instrument (GE Healthcare Life Sciences, USA). To avoid potential further unfolding of mutant protein we directly analysed them in imidazole-containing buffer A without previous dialysis ...
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bioRxiv - Genomics 2021Quote: ... as follows: The proteinase K-treated reaction was combined with 1 μL of Sera-Mag carboxylate-modified magnetic particles (GE Healthcare, Chicago, IL, USA.) and 72 μL of binding buffer (300 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 1:1000 diluted Amersham ECL anti-rat IgG horseradish peroxidase-linked species-specific whole antibody (NA935, GE Healthcare UK Limited, UK) or Amersham ECL anti-mouse IgG horseradish peroxidase-linked species-specific whole antibody (NA931 ...
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bioRxiv - Immunology 2021Quote: ... The Fc and IdeS A were removed using a mix of Protein A Sepharose® Fast Flow (250 μL per 1 mg digested mAb; GE Healthcare Life Sciences) and Ni Sepharose™ 6 Fast Flow (50 μL per 1 mg digested mAb ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with 0.1% PBS-T and were incubated with secondary horseradish peroxidase-coupled antibodies for 1h at RT (GE Healthcare, Jackson ImmunoResearch, 1:10000). ECL Western Blotting Detection Reagent (RPN2106 ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were washed (3x) with TBS-T and probed with the species-matching secondary horseradish peroxidase-conjugated antibody: donkey anti-rabbit (GE Healthcare, dilution 1:10.000) or sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... and membranes were incubated with secondary antibodies conjugated to horseradish peroxidase (anti-mouse or anti-rabbit; GE Healthcare 17097199 and 16951542, 1:5000 dilution) for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 5 times with PBS-T and incubated with a 1:5000 dilution of anti-mouse IgG-HRP (GE healthcare cat. # NA931-1ml), or with a 1:100000 dilution of anti-ferret IgG-HRP (Abcam cat ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... DTX3L and Flag epitope for 1 h at 4°C before Protein G Sepharose beads (30 μL per reaction mixture; GE Healthcare #GE17-0618-01) were added for 12 h ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc portion and uncut IgG was removed by flowing through a 1 mL or a 5 mL MabSelect SuRe column (GE Healthcare #11-0034-95) in PBS (Fab flows through the column) ...
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bioRxiv - Immunology 2023Quote: ... and processed within 2 hours by dilution at 1:1 (v/v) ratio in phosphate-buffered saline solution (PBS) with layering on top of 1 volume of Ficoll-Paque Premium (GE Healthcare, Chicago, IL, USA) and separation by density centrifugation at 500× g for 30 minutes (20°C ...
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bioRxiv - Biochemistry 2021Quote: ... diluted to 1 mL with buffer B and added immediately onto an anion exchange resin (Q resin; GE Healthcare; 0.2 mL; equilibrated in buffer H) and incubated (5 min) ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were subsequently incubated with the indicated primary antibodies followed by the appropriate horseradish peroxidase-labelled antibodies (1/10000, Sigma-Aldrich or GE Healthcare, see Supplementary table). Immunodetection was performed using Super Signal West Pico or Femto Chemiluminescence Kit (Pierce) ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed with PBST and incubated with alkaline phosphatase-conjugated secondary antibodies (anti-rabbit IgG and anti-mouse IgG, GE healthcare life sciences, 1:10000) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were transferred to an activated (100% Ethanol, 1 min; followed by two washing steps with water) PVDF membrane (GE Healthcare, Buckinghamshire, UK; Cat# 10600023) at 20V for 45 min ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Membranes were washed and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and detected by chemiluminescence system (GE Healthcare Europe GmbH, Freiburg, Germany).
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bioRxiv - Immunology 2019Quote: ... Cells were harvested and lysed in PBS supplemented with 1 mM PMSF and soluble protein was purified using nickel-affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare, Piscataway, New Jersey, USA). After SDS-PAGE analysis ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1 to 2 mL culture volume at a determined OD730 (between 8 and 10) were filtered onto pre-dried and pre-weighed glass microfiber filters (47 mm diameter, 1 µm pore size, GE Healthcare, Cat. No. 1822-047), washed twice with deionized water and dried overnight at 65 °C (until mass deviation between readings was no higher than 0.0001 g) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Zoology 2019Quote: ... The gDNA concentration was estimated by Qubit and about 20 ng of gDNA amplified with primers specifically designed to include the intron region (Hsp70 genomic F/R, Table 1) using PuReTaq Ready-To-Go™ PCR Beads (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Bioengineering 2019Quote: ... cells debris were removed by centrifugation at 16,000 × g for 20 min at 4 °C and the supernatant (supplemented with 20 mM Imidazole) was loaded on a 1 mL Ni+-HisTrapHP column (GE Healthcare #71-5027-68 AK) and washed with a buffer containing 100 mM Tris-HCl (pH 7) ...
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bioRxiv - Molecular Biology 2019Quote: ... approximately 30 μg of total RNA was denatured and separated on a 1% denaturing formaldehyde agarose gel and transferred to a Hybond-N+ nylon membranes (GE Healthcare Life Sciences, London, UK). PCR products were labeled with DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Microbiology 2019Quote: For the biomass evaluation in the AD7 medium optimization experiment 0.4 to 1 mL of cell cultures were filtered onto pre-dried and pre-weighed glass microfiber filters (47 mm diameter, 1 µm pore size, GE Healthcare, Cat. No. 1822-047), washed three times with MilliQ water in order to remove salt remains and dried for 24 hours at 65 °C (at which point measured weight did not differ by more than ± 0.0001 g).