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Citations for GE Life Sciences :
2151 - 2200 of 2314 citations for 8 Chloro 2 chloromethyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The clear supernatant was filtered (0.22-μm pore size) and loaded onto a 1-ml HisTrap HP column (GE Healthcare Life Sciences) equilibrated with low-KCl buffer A ...
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bioRxiv - Biophysics 2021Quote: ... The suspension was incubated up to 1 h and the non-coupled free dye was removed by exclusion chromatography using a PD10 exclusion column (GE Healthcare, Sweden) equilibrated with 50 mM NaH2PO4/Na2HPO4 with 1% glycerol.
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated secondary antibodies were incubated for 1 h at room temperature and membrane development was performed using Enhanced Chemiluminescence (ECL) Western Blotting substrate (GE healthcare, RPN2106) or ECL Prime Western Blotting substrate (GE healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated with horseradish peroxide (HRP)-conjugated) anti-rabbit IgG or anti-mouse IgG secondary antibodies (1:10,000, GE Healthcare Life Science) at room temperature and proteins were detected using Pierce ECL Western Blotting Substrate (34095 ...
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bioRxiv - Immunology 2022Quote: Purified protein samples at a concentration of 0.5 mg ml-1 were injected onto a Superdex Increase 10/300 GL column (GE Healthcare, Diegem, Belgium) that was pre-equilibrated with HBS ...
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bioRxiv - Developmental Biology 2021Quote: ... The immunoblots were washed and incubated for 1 h at RT with HRP-linked secondary antibody donkey anti-rabbit IgG (GE Healthcare, NA934). The HRP-signal was detected using SuperSignalTM West Pico PLUS Chemiluminescent Substrate Kit (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... After the restriction enzyme digestion step and the adapter ligation, large cutoff bead-cleanups (0.6:1, bead:library ratio) with homemade magnetic beads (Sera-Mag SpeedBeads™, #65152105050450, GE Healthcare Life Sciences) in PEG/NaCL buffer89 were used to clean the samples from the buffers and remove large fragments above ∼600 bp length ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used: donkey anti-rabbit IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK) and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... The mantle cell lymphoma cell line Maver-1 (#ACC 717, DSMZ Gmbh, Braunschweig, Germany) was cultured in HyClone™ RPMI 1640 medium (GE Healthcare) supplemented with 10% heat-inactivated FBS and 1% Penicillin/Streptomycin.
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bioRxiv - Cell Biology 2021Quote: ... was diluted 1:1 with phosphate-buffered saline (PBS) and separated to obtain buffy coat by density gradient centrifugation over Ficoll® Paque (GE Healthcare). The buffy coat ...
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bioRxiv - Cell Biology 2022Quote: ... The Glo3 BoCCS and GRM regions and β’-COP (residues 300-604) were ligated into BamHI/ NotI sites of pGEX-6P-1 (GE Healthcare), resulting in an N-terminal GST-tagged proteins with a 3C-protease cleavage site ...
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bioRxiv - Bioengineering 2019Quote: ... The supernatant was collected for purification using a 1 mL His Trap™ FF Crude Column containing Ni-NTA (GE Healthcare, USA). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... and the clear supernatant was filtered (0.22-μm pore size) before being loaded onto a 1-mL HisTrap HP column (GE Healthcare Life Sciences) equilibrated with HisTrap binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... The sample (1 ml of protein with a concentration of 0.5 mg ml-1) was loaded onto a Superdex™ 75 Increase 10/300 GL (GE Healthcare Lifesciences) column at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL, GE Healthcare, www.gehealthcare.com) of an Äkta Avant System (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... a stabilized soluble version of the spike protein (based on GenPept:QHD43416 ORF amino acids 1–1207) was produced using an ExpiCHO Expression system (Thermoscientific, USA, Cat. No. A29133) following purification using HisTrap (GE Healthcare, U.K.) and Strep-TactinXT (IBA ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were separated by electrophoresis using a 1% agarose gel and transferred to nylon membranes (Hybond N+®, GE Healthcare), where hybridization was carried out using an 812 bp fragment of Micropia from D ...
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bioRxiv - Biochemistry 2021Quote: ... isolated E coli membranes with overexpressed MgtA were solubilized in 1% β-dodecyl maltoside (β-DDM) prior to Histrap HP (GE Healthcare) purification equilibrated in (25 mM HEPES pH 7.0 ...
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bioRxiv - Immunology 2020Quote: ... Lysates were centrifuged at 14,000 rpm and proteins purified by affinity chromatography using a Ni2+ column (1 mL GE Healthcare HisTrap HP) and imidazole gradient elution in an AKTA Start protein purification system ...
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bioRxiv - Microbiology 2021Quote: ... ATP and MgCl2 were then added to a final concentration of 1 and 20 mM and then applied to a HiTrap Q column (GE Life Sciences). Separation occurred over an 18 CV gradient from 100% IEX1 to 100% IEX2 buffers [20mM Tris pH 8.0 (RT ...
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bioRxiv - Immunology 2020Quote: SARS-CoV and SARS-CoV-2 ectodomain and RBD constructs were transiently transfected in Expi293F cells at a density of 2 million cells/mL using polyethylenimine and expression plasmids at a molar ratio of 3:1 and purified from clarified transfected culture supernatants 4-days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Proteins were eluted from the column using 250 mmol/L imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by addition of 6 mM β-mercaptoethanol and mixtures were applied to a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by buffer exchange into G buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1 mM EDTA) using desalting column (GE Healthcare; cat#17085101). To remove the GST tag ...
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bioRxiv - Cell Biology 2022Quote: ... Extract was cleared by centrifugation at 186.000 x g for 1 h at 4°C and then incubated at 4°C with Glutathione Sepharose™ 4B resin (GE Healthcare) for 3h ...
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bioRxiv - Biochemistry 2022Quote: ... The complex formed in the 1:2.5 mixture was purified on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in assay buffer and run at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the patients underwent a T1 weighted 1 mm isometric anatomical MRI scan using a 3T Signa HDx scanner (GE Healthcare, Chicago, Illinois). Following the implant ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl of each mucus sample was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA), left to dry out ...
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bioRxiv - Neuroscience 2022Quote: ... Images were captured one frame every 1 min 30 sec over 5 h using a DeltaVision Imaging System (Applied Precision, Bratislava, Slovakia).
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bioRxiv - Molecular Biology 2022Quote: ... 10 or 15 % 29:1 acryl:bis-acrylamide) and transferred on Amersham™ Protran™ 0.45 μm nitrocellulose membranes (GE Healthcare, Cat# 10600033) at 4°C for 1 hr at 110V ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were then washed in PBT and incubated with HRP conjugated-anti-rabbit IgG (GE Healthcare # NA9340, 1:5000 in blocking solution) at room temperature for 1 hour and washed twice in PBT ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was then diluted to a final salt concentration of low salt buffer conditions (100 mM KCl, 50 mM HEPES, 1 mM MgCl2) and bound to HiTrap SP FF (GE Healthcare, USA) anion exchange column ...
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bioRxiv - Cell Biology 2024Quote: ... The tagged proteins were detected using HRP conjugated sheep anti-mouse or anti-rabbit IgG antibody (1:10000, GE Healthcare, Uppsala, Sweden) and visualized using the Amersham ECL Western blot detection reagent (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... plasma or CSF probes were incubated in 25 μl for 1 hour at room temperature (diluted with PBS containing 5% Amersham ECL Blocking Agent, GE Healthcare RNP2125), followed by additional washing steps and incubation with an anti-mouse secondary antibody conjugated with the MSD Sulfo-tag for detection ...
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bioRxiv - Genetics 2023Quote: ... was incubated with recombinant GST-DOCK3-PXXP or GST alone plasmids (constructs cloned into pGEX-6P-1 plasmid; GE Healthcare; Chicago, IL) in GST reaction buffer (250 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... CaM was eluted in buffer containing 1 mM EDTA then further purified using anion exchange with a Resource Q column (GE Life Sciences) before dialysis into water and lyophilization in a vacuum concentrator.
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed 3×10mins with TBS-T and 1×10mins with TBS before developing the signal by incubating the membrane in Amersham Hyperfilm ECL for 5 minutes (GE Healthcare, UK).
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was washed 5 times in PBS-T before it was incubated with the secondary antibody goat α-mouse IgG (H&L)-HRP (1:10’000, GE healthcare #NXA931). After 1 h incubation ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were injected two times/embryo with mRNAs at two-cell or four-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNAs or plasmid DNA at two-cell or four-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...
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bioRxiv - Immunology 2022Quote: Serum samples from HIV-1-infected individuals were heat-inactivated at 56 °C for 40 min and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... equivalent to ∼1-3×108 cells per sample) were collected by vacuum filtration onto glass microfiber filters (grade GF/A, GE Healthcare Whatman), immediately plunged into liquid nitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-3xFLAG-V1H(1-351) was further purified by gel filtration on a Superdex 200 10/300 GL column (GE Life Sciences) into the appropriate buffer for the subsequent application ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of fosmid or BAC DNA was labelled by nick-translation with fluorescent dUTPs (Abott Molecular or GE HealthCare Life Science) for 3 h at 15°C as described previously41 ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was diluted 1:10 v/v with 20 mM Tris 8.0 and further purified with anion exchange chromatography (HiTrapQ column, GE Healthcare Life Sciences) using a NaCl gradient of 50 mM to 500 mM in 20 mM Tris 8.0 ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was washed with 0.1% TBST and incubated with corresponding secondary antibody conjugated to Horse Radish Peroxidase (HRP) at 1:10000 (GE life sciences; NA934) dilutions for one hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and detected with a 1:10000 goat anti rabbit secondary antibody conjugated with HRP and imaged with ECL (GE healthcare NA931, NA934).
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bioRxiv - Genetics 2024Quote: ... Cell debris was removed by centrifugation and the supernatant was incubated with 1 mL of equilibrated Ni-Sepharose 6 Fast Flow resin (GE Life Sciences) and gently mixed in batch for 1 hr at 4° C ...
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bioRxiv - Microbiology 2020Quote: ... This reaction was incubated at 37°C for 1 hour and cleaned up with ProbeQuant G50 spin columns (GE Healthcare, GE28-9034-08) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... was purified using an ÄKTAprime plus fast protein liquid chromatography (FPLC) system equipped with a 1-mL His-Trap HP column (GE Healthcare-Amersham Biosciences) and the high salt buffer ...