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Citations for GE Life Sciences :
2201 - 2250 of 4722 citations for Recombinant Human TFPI Protein His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The labelled proteins were further purified by size-exclusion chromatography (Superdex 200, GE Healthcare) using buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... Phosphorylated proteins were visualized using an FLA7000IP Typhoon Storage Phosphorimager (GE Healthcare Life Sciences) and quantified in ImageJ ...
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bioRxiv - Neuroscience 2019Quote: ... Polyclonal antibody 2134 supernatent was purified using a Protein A affinity column (GE Healthcare). Serum was neutralized with 1/10 volume 1 M Tris pH 8 ...
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bioRxiv - Biophysics 2019Quote: ... The diluted protein was applied to a heparin column (HiTrap Heparin HP, GE Healthcare), washed with 20mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was further purified by cation exchange with Source S column (GE Healthcare), eluted with a linear KCl gradient of 0.1 – 1 M KCl ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were then incubated with protein G sepharose beads (GE Healthcare, 17-0618-01) for 2–3 h at 4°C and beads were washed 3 times with diluted lysis buffer (dilution of 1:3 in dH2O ...
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bioRxiv - Plant Biology 2019Quote: ... The secreted protein was purified from the supernatant by Ni2+ (HisTrap Excel; GE healthcare; equilibrated in 50 mM KPi pH 7.6 ...
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bioRxiv - Molecular Biology 2019Quote: ... The diluted protein was applied to a heparin column (HiTrap Heparin HP, GE Healthcare), washed with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were electrophoretically transferred onto nitrocellulose membranes (Amersham Protran 0.45 μm NC; GE Healthcare) at 100 V for 180 min on ice in the transfer buffer without SDS/EDTA ...
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bioRxiv - Plant Biology 2019Quote: ... and the separated protein bands were electroblotted onto a polyvinylidene difluoride membrane (GE Healthcare). Different amount of proteins were used for each western blotting ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were electrophoretically transferred onto nitrocellulose membranes (Amersham Protran 0.45 μm NC; GE Healthcare) at 100 V for 180 min on ice in transfer buffer [48 mM Tris/HCl ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were electrophoretically transferred onto nitrocellulose membranes (Amersham Protran 0.45 μm NC; GE Healthcare) at 90 V for 90 min on ice in transfer buffer [48 mM Tris/HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... The dialyzed protein was resolved on a HiTrap SP FF 5mL column (GE Healthcare) with a linear gradient between buffer A (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... The mAbs were purified using HiTrap Protein A HP column (GE Healthcare, Chicago, IL), followed by size exclusion chromatography (Superdex 200 16/90 ...
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bioRxiv - Neuroscience 2020Quote: Gels were silver stained by using the PlusOne Silver Staining Kit (protein, GE Healthcare) to excise the selected spots following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Subsequently proteins were immobilized on a Ni Sepharose 6 Fast Flow resin (GE Healthcare). The resin was incubated 2-4 h at 4°C with 25 nmol of each fluorophore dissolved in labelling buffer 1 (50 mM Tris-HCl pH 7.4-8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Phosphorylation of substrate proteins was visualized using autoradiography (Typhoon 9210; GE Healthcare Life Sciences). Autoradiographs were quantified with the ImageQuant TL Software (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2020Quote: ... The reactive proteins were detected using Image Quant LAS500 software (GE Healthcare Life Sciences).
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bioRxiv - Pathology 2020Quote: ... Proteins were transferred to the Hybond-PVDF-LFP membrane (Amersham, GE Healthcare Life Sciences) and blocked with 5% skimmed milk in 1X Tris-buffered saline ...
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bioRxiv - Cell Biology 2020Quote: ... the protein was buffered into PBS using a NAP5 column (GE Healthcare, Freiburg, Germany).
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bioRxiv - Molecular Biology 2019Quote: ... The protein solution was loaded on a Superdex200 pg 16/60 column (GE Healthcare) and eluted in ClpX SEC buffer (25 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... the supernatant was pre-cleared by incubation with protein G sepharose beads (GE Healthcare) on a rotating platform for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was then loaded on a HiTrapQ HP anion exchange column (GE Healthcare) and eluted in a gradient from 120–600 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were purified by affinity chromatography using a GSTrap HP affinity column (GE Healthcare) and size exclusion chromatography (HiLoad S200 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins purification procedures were performed using the chromatographic system Akta Purifier (GE Healthcare, USA).
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bioRxiv - Cell Biology 2019Quote: ... The proteins were separated and transferred to PVDF membrane (GE Healthcare Cat No-10600023) using semi-dry transblot apparatus (Hoefer ...
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bioRxiv - Biochemistry 2021Quote: ... and size exclusion chromatography via an ÄKTA fast protein liquid chromatography system (GE Healthcare). Purity of proteins was determined by SDS-polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated by SDS-PAGE and were transferred onto PVDF membranes (GE Healthcare) for immunoblot analysis ...
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bioRxiv - Immunology 2020Quote: ... IgG was purified with a HiTrap MabSelect SuRe protein A affinity column (GE Healthcare) on an AKTA Pure (GE Healthcare) ...
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bioRxiv - Immunology 2019Quote: ... MAbs were purified over Protein G Sepharose 4 Fast Flow resin (GE Healthcare; 17061802). The antibodies were buffer exchanged into PBS pH 7.4 prior to use in any cell based assays.
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bioRxiv - Biophysics 2019Quote: ... The labelled proteins were further purified by size-exclusion chromatography (Superdex 200, GE Healthcare) using buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... OCN fusion protein was digested with thrombin (27-0846-01, GE Healthcare Life Sciences) and eluted with 1× PBS ...
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bioRxiv - Biochemistry 2019Quote: ... The tag-free proteins were further purified by anion exchange chromatography (MonoQ; GE Healthcare). Peak fractions were collected ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were immobilized on the Series S Sensor Chip Protein A chip (GE Healthcare), and then SARS-CoV-2-RBD-his protein with a gradient concentration from 1.25 nM to 40 nM was injected into the chip ...
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bioRxiv - Biochemistry 2021Quote: ... Gel containing fluorescent protein samples were scanned with Typhoon FLA 9500 imager (GE Healthcare), using 473 nm blue LD laser/LBP (510LP ...
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bioRxiv - Immunology 2021Quote: ... affinity chromatography column using an ÄKTATM Fast Protein Liquid Chromatography (FPLC) instrument (GE Healthcare). The column was washed with 1X PBS ...
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bioRxiv - Immunology 2021Quote: ... Expressed protein was isolated by metal affinity chromatography on HisTrap Excel columns (GE Healthcare), followed by GraFix methods using a 10% to 30% glycerol gradient and 0 to 0.1% glutaraldehyde gradient (Stark ...
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bioRxiv - Physiology 2020Quote: ... The separated proteins were electro-transferred to PVDF membranes (GE healthcare, Cat no. 10600021). These were subsequently blocked with 5% milk in Tris-buffered saline with 0.1% Tween 20 (TBST ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were further purified by a Superdex 200 gel filtration column (GE Healthcare) equilibrated in the buffer containing 25 mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... Then proteins were transferred onto an Amersham Protran western blotting nitrocellulose membrane (GE Healthcare) by semi-dry transfer ...
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bioRxiv - Cell Biology 2021Quote: ... The protein solution was then loaded on an HiTrap IMAC HP column (GE Healthcare). Further purification of the Klp9-containing fractions was achieved by gel filtration using a Superdex 200 26/60 column equilibrated with a buffer containing 20 mM HEPES pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... protein sample was extracted from the supernatant with a HisTrapTM HP column (GE healthcare) and eluted with an imidazole gradient ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein was further purified using a Hitrap Q HP column (GE Healthcare) equilibrated in buffer B (25mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2021Quote: ... the L proteins were further purified by size-exclusion chromatography (Superose 6, GE Healthcare) in buffer B ...
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bioRxiv - Biochemistry 2021Quote: 100 μL protein samples were injected onto an S200 10/30 column (GE Healthcare) equilibrated in a running buffer of 10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... 650μg-1mg of the lysate was added to Protein A-Sepharose beads (GE Healthcare) pre-incubated for 3-7 h with rabbit anti-FLAG antibody (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... Partially purified protein was further loaded onto a 5-ml Heparin column (GE Healthcare) pre-equilibrated in a low-salt wash buffer and eluted with increasing salt gradient to 1 M NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Dialyzed proteins were loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare) and eluted with a linear gradient of 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... the protein was further purified by Mono-S anionic exchange (GE Healthcare, Marlborough, MA) chromatography.
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bioRxiv - Biophysics 2021Quote: ... The protein was purified using Ni2+-NTA affinity chromatography of AKTATM Pure (GE healthcare). 20 mM imidazole containing binding buffer was used for the proteins for attaching to the column which was later eluted by 250 mM imidazole containing buffer ...