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Citations for GE Life Sciences :
2001 - 2050 of 4591 citations for Recombinant Human TFPI Protein His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Then proteins were transferred to a nitrocellulose membrane (Whatman, GE Healthcare Life Sciences). After transfer ...
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bioRxiv - Biophysics 2024Quote: ... the protein was passed through a 5 mL HisTrap HP column (GE Healthcare), and the cleaved protein was collected in the flow through ...
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bioRxiv - Synthetic Biology 2023Quote: ... Further protein cleanup and tryptic digest were performed with SP3 beads (GE Healthcare), using the SP3 protocol described in reference (5) ...
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bioRxiv - Microbiology 2023Quote: ... The protein was then loaded onto a HiTrap Q HP Column (GE Healthcare) and eluted using a gradient from buffer QA (50 mM Tris ...
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bioRxiv - Genetics 2023Quote: ... The cleaved protein was concentrated and applied to a Superdex 75 (GE Healthcare) pre-equilibrated with 20 mM Tris-HCl pH 7.8 and 150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... blotted proteins were visualized with an ECLTM Prime Western Blotting System (GE Healthcare). All expression levels were normalized to β-actin expression.
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Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopybioRxiv - Cell Biology 2024Quote: ... Immunoreactive proteins were detected with ECL Prime Western Blotting Detection Reagent (GE Healthcare) using an ImageQuant LAS 500 device (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... the supernatants were combined with Protein G Sepharose beads (GE Healthcare, Illinois, USA) and incubated at 4 °C for an hour ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified using size exclusion chromatography (Superdex S75; GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... and the protein was further purified by Q Sepharose column chromatography (GE Healthcare) and gel-filtration column chromatography (HiLoad Superdex75 16/60 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were detected using a luminescence image analyzer (LAS-4000 mini; GE Healthcare) or a luminescence image analyzer (FUSION Solo S ...
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bioRxiv - Biophysics 2024Quote: ... we loaded the protein solution onto a HiTrap Q FF column (GE Healthcare) and eluted it with a linear gradient of 0–1 M NaCl in 20 mM sodium phosphate at pH 6.0 ...
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bioRxiv - Biochemistry 2024Quote: ... eluted protein was loaded onto a HiPrep 26/10 desalting column (GE Healthcare) containing 25 mM Tris pH 7.5 at 20 °C ...
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bioRxiv - Immunology 2024Quote: ... Lysate pre-cleaning steps were performed using Protein G Sepharose beads (GE Healthcare). To measure the protein content of the lysate a Qubit 4 fluorometer was used using the Qubit protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Protein bands reacting with antibodies were visualized using chemiluminescent detection reagent (GE Healthcare) on an iBright FL1500 (ThermoFisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... The NRP2-Fc was purified using a Protein-A affinity column (GE Healthcare). NRP2-Fc point mutations were introduced by PCR mutagenesis using Kapa HiFi DNA polymerase (Kapa Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... supernatants were harvested and IgG was purified on Protein G Sepharose (GE Healthcare)
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bioRxiv - Plant Biology 2023Quote: ... GST fusion proteins were purified on Glutathione-Sepharose® 4B beads (GE Healthcare) and eluted using a solution of 50LmM Tris-HCl pH 8 ...
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bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were isolated from healthy volunteer donors by layering over Ficoll-Paque Plus (GE Healthcare) as per the manufacturer’s instructions and were enriched for CD4+ or CD8+ T cells using RosetteSep (STEMCELL Technologies ...
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bioRxiv - Microbiology 2020Quote: Human PBMCs were isolated from leukapheresis obtained from the Central Blood Bank (Pittsburgh, PA) using Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2021Quote: ... Human IgGs were isolated from heat-inactivated plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences) as described (24).
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bioRxiv - Cell Biology 2022Quote: Fibronectin was purified from outdated human plasma (American Red Cross, Rochester, NY) using gelatin-Sepharose (GE Life Sciences, now Cytiva) affinity chromatography (102) ...
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bioRxiv - Microbiology 2021Quote: ... A549 and human colon carcinoma LoVo cells (both purchased from ATCC) were cultured in F12 Kaighns Modification media (GE Healthcare) with 10% FBS and 1% penicillin/streptomycin.
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bioRxiv - Immunology 2021Quote: ... Human peripheral blood mononuclear cells were isolated from fresh whole blood by Ficoll-Paque (GE Healthcare, Little Chalfont, United Kingdom). CD4+CD45RA+ naïve T cells were isolated using negative enrichment kits (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: ... Human and mouse GSDMD were further purified by gel filtration chromatography using a Superdex200 (16/60 GL) column (GE Healthcare) in a running buffer containing 20 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... Human MSLN (Advance BioMatrix 5123-0.1mg) was (10 mg/mL) was immobilized onto a CM5 sensor chip (GE Healthcare, BR100012) by amine coupling ...
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bioRxiv - Molecular Biology 2023Quote: ... monocyte or a macrophage were isolated directly from human whole blood using Ficoll-Paque (Premium, GE Healthcare, Chicago, IL, USA) density gradient centrifugation ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells were isolated from screened buffy coats by density centrifugation with Ficoll-Plaque Plus (GE Healthcare) followed by magnetic-assisted cell sorting ...
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bioRxiv - Plant Biology 2020Quote: ... Secreted proteins were purified from the supernatant by sequential Ni2+ (HisTrap excel; GE Healthcare; equilibrated in 25 mM KPi pH 7.8 ...
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bioRxiv - Biophysics 2020Quote: ... The filtrate was analysed by fast protein liquid chromatography (AKTApurifier, GE Healthcare Life Sciences) using a MonoQ column (5/50 GL ...
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bioRxiv - Biophysics 2020Quote: ... the protein was separated on a RESOURCE™ reversed phase chromatography column (GE Healthcare) using a 0-70 % acetonitrile gradient ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were loaded onto an Äkta Pure chromatography setup (GE Healthcare, Uppsala, Sweden) equipped with a Superdex 200 26/60 column or Superdex 200 16/60 column (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... was bound to 20 μL (bed volume) FastFlow Protein A Sepharose beads (GE Healthcare) by incubating for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and the GST-fusion protein was purified using Glutathione-Sepharose affinity columns (GE Healthcare). The proteins were finally eluted in PBS containing 20 mM glutathione (pH 8 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was concentrated by centrifugal ultrafiltration and applied to a Superdex-200 (GE Healthcare) size exclusion chromatography column ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using the 2-D Quant kit (GE Life Sciences 80648356). Samples were reduced (5 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected by enhanced chemiluminescence (ECL Prime Western blotting detection reagent; GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... filtered and purified using protein G affinity chromatography following the manufacturer’s protocol (GE Healthcare).
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bioRxiv - Genetics 2021Quote: ... Protein bands were visualised using Enhanced Chemiluminescence (ECL) Western blotting detection reagents (GE Healthcare) and exposure of the membrane to Kodak BioMax XAR film (Sigma) ...
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bioRxiv - Genetics 2021Quote: ... and proteins were transferred to a nitrocellulose membrane (Amersham Protran 0.45 NC, GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected by enhanced chemiluminescence (ECL Prime Western blotting detection reagent, GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated on a HiLoad Superdex 200 Increase 10/300 column (GE Healthcare) and plotted against BioRad Standards to determine size ...
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bioRxiv - Plant Biology 2021Quote: The purified proteins were loaded onto a Superdex 200 GL 10/300 (GE Healthcare) column and eluted with a buffer containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Developmental Biology 2020Quote: ... and proteins were detected using Amersham Western Blotting Detection Reagent (GE Healthcare, Chicago, IL). Western blot membranes were probed with mouse anti-RFP (RF5R Invitrogen ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... Protein was detected using an Amersham ECL Western Blotting Analysis System (GE Healthcare RPN2109), and developed onto film or imaged using a Biorad Chemidoc gel imager ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein purification was performed using Nickel NTA columns (17-5248-01, GE Healthcare, USA) or beads (7066-4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Fc and non-digested IgG were removed via Protein A affinity chromatography (GE Healthcare). 2E10.E9 Fab was subsequently purified via size-exclusion chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Molecular Biology 2020Quote: ... Then the target proteins were loaded onto a HiTrap Q column (GE Healthcare, USA). An increasing linear gradient of NaCl concentration was applied onto the HiTrap Q column for further purification ...
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bioRxiv - Molecular Biology 2021Quote: ... Total protein levels were quantified with the ImageQuant TL software (GE Healthcare Life Sciences).
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bioRxiv - Molecular Biology 2020Quote: ... protein was immobilized to the CM5 sensor chip (GE Healthcare, Cat#. BR-1005-30) at 6.0 μg/mL concentration using 10mM sodium acetate (pH 5.5 ...