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Citations for GE Life Sciences :
2151 - 2200 of 4746 citations for GATOR2 complex protein WDR59 WDR59 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Further purification of S-nitrosylated proteins were done using Thiopropyl Sepharose 6B bead (GE Healthcare# 170420001) in the presence of 50mM Na-ascorbate as a mild reducing agent ...
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bioRxiv - Immunology 2021Quote: ... Protein bands were visualised using Amersham™ Imager 600 (GE Healthcare Life Sciences, Malborough, MA, USA).
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bioRxiv - Microbiology 2021Quote: ... Protein-containing fractions were pooled for desalting through a 5 mL HiTrap Desalting column (GE Healthcare). Desalted protein was dialyzed into a storage buffer (50 mM NaH2PO4 pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant protein was purified by chromatography using a Nickel-loaded Hitrap Chelating HP column (GE Healthcare). Unbound material was extensively washed using Tris 20mM pH8 ...
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bioRxiv - Developmental Biology 2020Quote: ... and GST-tagged target protein was purified using Glutathione Sepharose 4B (GE Healthcare, Marlborough, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Immuno-detected proteins were visualized by chemiluminescent photographic film exposure (ECL kit; GE Life Sciences, RPN2232) and digitally scanned using a GS-800 calibrated densitometer (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was then purified by ion exchange chromatography (mono Q 10/100 GL, GE Healthcare), followed by SEC using a Superdex 200 increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) with PBS as the running buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) with PBS as the running buffer ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-fusion proteins were coupled to Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 2 h at 4 °C with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was subjected to size-exclusion chromatography on a HighLoad 16/60 Superdex75 (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... protein-RNA complexes were immobilized on Protran nitrocellulose membrane (0.45 μm pore size, Whatman, GE Healthcare) and unbound RNA immobilized on Hybond Nylon membrane (Amersham ...
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bioRxiv - Molecular Biology 2020Quote: ... 1500 µg protein extract containing GST-Gal1p was loaded on a GST SpinTrap column (GE Healthcare) and incubated for 1.5 hour at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... Fc fragments and undigested IgG were removed from binding to the protein A beads (GE Healthcare). CC12.1 and eCC12.1.7 Fab fragments were generated by introducing a stop codon after the CH1 region in heavy chain expression plasmids ...
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bioRxiv - Biophysics 2022Quote: ... The protein was further purified by anion-exchange chromatography on a Mono-Q column (GE healthcare). Expression and purification of RT600 was carried out in a similar way as with RT139A except that the 6x-His tag on p51 N-terminus was not cleaved ...
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bioRxiv - Biophysics 2022Quote: ... Protein was then affinity purified from lysate using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer (100 mM tris-HCl ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: ... Eluted protein was concentrated and subjected to a HiLoad 16/600 Superdex 75 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES ...
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bioRxiv - Plant Biology 2020Quote: ... Separated proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, United Kingdom) using a wet tank unit (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... and were purified using HisTrap HP column and ÄKTA protein purification system (GE Healthcare Life Sciences). For crystallization ESK1 samples were further purified using Source 15Q anion exchange chromatography with 20 mM Tris pH 7.5 and 0 to 0.35 M NaCl gradient ...
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bioRxiv - Pathology 2020Quote: ... Tau protein was further purified by FPLC using size exclusion Superdex75 and Superdex200 columns (GE Healthcare) in 1X PNE buffer (25 mM PIPES pH 7.0 ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... and the eluted protein was further purified through 5 mL HiTrap Heparin HP column (GE Healthcare). Finally ...
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bioRxiv - Microbiology 2020Quote: ... The resolved BBMV proteins were transferred to a nitrocellulose filter (Protran 0.45 μm NC, GE Healthcare) using a BioRad Mini Trans-Blot system at 4°C in blotting buffer (39 mM Glycine ...
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bioRxiv - Immunology 2021Quote: ... The protein was further purified on a pre-equilibrated Superdex 75 gel filtration column (GE Healthcare). Fractions containing monomeric ...
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bioRxiv - Cell Biology 2020Quote: ... labelled protein was purified using gel-filtration chromatography on Superdex 200 10/300 column (GE Healthcare) in buffer containing 50 mM HEPES pH=8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... The labeled proteins were further purified using the Superdex 200 10/300 columns (GE Healthcare, USA). As for TDP-43 LCD ...
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bioRxiv - Biophysics 2020Quote: ... the protein was again subjected to gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences). Monomeric fractions of dSA were then concentrated (10kDa Amicon®Ultra-4 centrifugal filters ...
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bioRxiv - Biochemistry 2020Quote: ... overnight and protein was further purified over a Superdex 75 16/600 pd column (GE Healthcare) equilibrated in 50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare 10600019 or 10600001). Appropriate transfer of proteins was verified by staining of membranes with the Ponceau S solution (Roth 5938.1) ...
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bioRxiv - Bioengineering 2020Quote: ... The supernatants were purified using a peristaltic pump and a HiTrap Protein A column (GE Healthcare). Fc-fused proteins were acid-eluted into 1X PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified from the cell-free extract using an HisTrap HP column (GE Healthcare) and a linear imidazole gradient (30 to 500 mM ...
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bioRxiv - Biochemistry 2021Quote: ... and protein loaded onto a GSTrap FF 5 mL column (GE Life Sciences, Marlborough, MA, USA) using the NGC Chromatography system (Bio-RAD) ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was fractionated on a HiLoad 16/600 Superdex 200 gel filtration column (GE Healthcare), eluting with SEC buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was loaded onto a Superdex 75 10/300 gel filtration column (GE Healthcare, USA), which had been equilibrated in buffer containing 20 mM Tris pH 8.0 and 100mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare). The protein was dialyzed against 1X PBS overnight in the presence of 1:1000 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare) in 1XPBS pH 7.5 and eluted with 300 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified using anion exchange chromatography (Mono Q 5/50 GL, GE healthcare) pre-equilibrated with ion exchange buffer A (20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The flow-through fraction containing target proteins were collected and purified with Superdex 75 (GE Healthcare) chromatography ...
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bioRxiv - Biochemistry 2020Quote: ... After electrophoresis the proteins were transferred to Nitrocellulose membranes (Amersham Protran Premium Nitrocellulose, GE Healthcare, #10600048) via wet transfer at a current of 45V for approximately 20 hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Eluted protein was further purified by size exclusion chromatography using a Superdex200 column (GE Life Sciences) in 25 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... Diluted protein was subjected to anion exchange chromatography using a HiTrapQ HP column (GE Life Sciences) and eluted in 20 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 μm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine) ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 μm NC) at 90 V for 90 min on ice in transfer buffer (48 mM Tris-HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Biophysics 2020Quote: ... cells were pelleted and the supernatant was loaded onto a HiTrap Protein A column (GE Healthcare) following the manufacturers protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... 35S-labelled proteins were monitored using autoradiography and visualised using a Typhoon 9400 scanner (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... The separated proteins were transferred to a 0.2 µm nitrocellulose membrane (Amersham Protran, GE Life Sciences) using wet transfer method for 2 h at 200 mA ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was visualized by Amersham enhanced chemiluminescence (ECL) Western Blotting Detection Reagent (GE Healthcare Life Sciences) and X-ray film (Fujifilm SuperRX) ...
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bioRxiv - Cancer Biology 2020Quote: ... Membrane-immobilized protein detection used ECL Western Blotting Detection Reagents (GE Healthcare RPN2106; Millipore Sigma GERPN2106).
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the mixtures were purified with Dextrin SepharoseTM High Performance MBP-tagged protein purification resin (GE Healthcare). Ten micrograms of proteins from elutes was resolved on 12% SDS-PAGE gels and visualized with Coomassie blue staining ...
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bioRxiv - Biochemistry 2021Quote: ... and protein fractions were separated using a Superdex 200 10/300 column (GE Healthcare Life Sciences) with 50mM Tris (pH 7.5 ...