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Citations for GE Life Sciences :
2051 - 2100 of 4746 citations for GATOR2 complex protein WDR59 WDR59 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... coli M55244 cells (ATCC) and purified using Protein L resin (GE Healthcare Cat no. 17547802) with gravity flow affinity chromatography ...
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bioRxiv - Biophysics 2022Quote: ... All proteins were further purified on a 5 ml HiTrap Heparin HP column (GE Healthcare), equilibrated in their respective dialysis buffer and eluted by an NaCl gradient containing 1 – 1.5 M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The target proteins were purified with HisTrap HP columns (GE Life Sciences, Velizy-Villacoublay, France). Further purification was achieved by size-exclusion chromatography in buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing desired protein was applied to either Glutathione-Sepharose resin (GE Healthcare Life Science) for GST purification or Ni-NTA Agarose resin (QIAGEN ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved protein was further purified using a HiTrap HP Heparin column (GE Healthcare, Illinois, USA), eluting with a linear gradient to 1.0 M KCl ...
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bioRxiv - Biophysics 2022Quote: ... The protein-containing supernatant was then applied to PD-10 Sephadex desalting column (GE Healthcare) to remove access of peptide/dye ...
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bioRxiv - Plant Biology 2022Quote: ... protein bands and radioactive bands were detected with an ImageQuant LA4000 imaging system (GE Healthcare) and a Typhoon Trio phosphorimager (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... The intensity of the resultant protein bands was quantified using ImageQuant TL software (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins in the supernatants were purified using HIStrap FF columns (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Protein was then purified from the supernatant using a StrepTrap HP column (GE Healthcare #28907547), followed by SEC purification over a S6 increase column (GE Healthcare #29091596 ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Cleaved recombinant protein was then purified on a MonoQ 5/50 GL column (GE Healthcare) using Buffer QA (described above + 1 mM DTT ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified on a 5 ml HiTrap SP HP cation exchange column (GE Healthcare) and eluted with 1 M KCl ...
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bioRxiv - Neuroscience 2023Quote: ... Flag immunoprecipitates of IGF-1R-Flag were incubated with Protein G Sepharose beads (GE Healthcare). Beads were pelleted ...
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bioRxiv - Biochemistry 2023Quote: ... The recombinant proteins were batch purified on Nickel-Sepharose beads 6 Fast Flow (GE Healthcare) and eluted with 6xHis-TEV protease ...
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bioRxiv - Biochemistry 2023Quote: ... Binding of the proteins was evaluated using an anti-GST-HRP conjugate (GE Healthcare, RPN1236). For comparative evaluation of affinities ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were pre-cleared by incubating with protein A or G sepharose beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... The spike proteins were further purified on a Superose 6 gel filtration column (GE Healthcare) in 25 mM Tris pH 7.5 + 150 mM NaCl using an AKTA Pure FPLC instrument (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... Purified protein samples were resolved on a Superdex200 Increase 10/300 GL column (GE Healthcare) connected to a high-performance liquid chromatography (HPLC ...
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bioRxiv - Biochemistry 2023Quote: ... Cas9 proteins were further purified by chromatography on Ni-NTA and SP column (GE Healthcare).
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bioRxiv - Plant Biology 2023Quote: ... these proteins were mixed with GST-LAZY4ΔCCL and incubated with Glutathione Sepharose 4B (GE Healthcare) at 4 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... and bound proteins were eluted by digestion with 20 units of Prescission protease (GE Healthcare) in 0.5 ml of Prescission protease cleavage buffer (50mM Tris-HCl pH 7.0 ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were purified with a Hiload 16/600 Superdex 200 pg column (GE Healthcare) preequilibrated with storage buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified using Glutathione Sepharose™ 4 Fast Flow (GE Healthcare #17-5132-03) and cleaved using 3C protease protein prior to reverse purification using glutathione beads followed by a HiLoad 16/60 Superdex 75 size exclusion chromatography.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Protein bands were visualized and imaged by an ImageQuant LAS 500 scanner (GE Healthcare, USA). After transfer ...
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 mm NC) at 90V for 90 min on ice in transfer buffer (48mM Tris–HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Plant Biology 2024Quote: ... GST-tagged or MBP-tagged proteins were captured by glutathione-agarose resin (GE Healthcare, 17075601) or amylose resin (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... the RTL4CV protein was detected with an ECL Prime Western Blotting Detection kit (GE Healthcare) using an anti-GFP antibody (MBL ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred at 70 V for 2 h onto a nitrocellulose membrane (GE Healthcare) using a wet electroblotting system (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... protein spots were transferred to Amersham Hybond-P poly(vinylidene difluoride) (PVDF) membranes (GE Healthcare) and the Western Blot (WB ...
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bioRxiv - Immunology 2024Quote: ... The eluted proteins were further gel filtered using a Superdex 200 Increase column (GE Healthcare) in storage buffer (50 mM HEPES-NaOH ...
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bioRxiv - Genomics 2024Quote: ... and 100 µg of protein was purified using a 2D Clean-up Kit (GE HealthCare) following the manufacturer’s instructions with the exception that 400 μl of precipitant and co-precipitant were used in the first step ...
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bioRxiv - Immunology 2024Quote: ... staining or alternatively by immunoblotting transferring proteins to nitrocellulose membrane (GE Healthcare, Chicago, IL, USA) and detection using a primary anti-Penta-His antibody (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... Protein expression levels were visualized with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences).
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bioRxiv - Biochemistry 2024Quote: ... the protein elution was further purified by Superdex 200 Increase 10/300 GL (GE Healthcare).
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bioRxiv - Genetics 2024Quote: ... Proteins were detected by chemiluminescence using ECL SelectTM Western blotting Detection reagent (GE Healthcare, AmershamTM) and a Fujifilm LAS-3000 camera.
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bioRxiv - Neuroscience 2024Quote: ... The proteins were cleaved from the GST resin by incubation with PreScission Protease (GE Healthcare) overnight at 4°C.
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bioRxiv - Plant Biology 2024Quote: ... Biotinylated proteins were pulled-down using Streptavidin Mag SepharoseTM (Cytiva 28-9857-99; GE Healthcare) and then submitted to on-bead digestion ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were purified by GST affinity chromatography on the glutathione Sepharose beads (GE Healthcare) in lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 mm NC) at 20 V for 60 minutes in transfer buffer (25 mM Tris base ...
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bioRxiv - Biophysics 2024Quote: ... The proteins were purified by affinity chromatography using an ÄKTA Start (GE Healthcare ÄKTA Start). The lysate was passed through a 5 mL pre-equilibrated HisTrap HP column (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: ... the recombinant protein GST-ZmSnRK2.10 or GST was purified using Glutathione Sepharose 4B (GE Healthcare) and separately incubated with glutathione beads 4°C for 2h ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was then applied to a HiTrap S HP (5 ml) column (GE Healthcare) equilibrated in low salt buffer ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were purified by Ni-NTA (2 x HiTrap Chelating HP, 5 mL, GE Healthcare), buffer exchanged to cleavage buffer (20 mM TrisHCl pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... The chromatography was performed using a fast protein liquid chromatography system (Äkta go GE Healthcare) at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) for 4h at 4°C with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... Target proteins were initially purified by immobilized metal ion affinity chromatography (Chelating Sepharose, GE Healthcare) following a standard protocol ...
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bioRxiv - Biochemistry 2024Quote: ... His-tagged proteins were visualized using the ECL Prime Western Blotting Detection Reagent (GE Healthcare) in a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... The GST-tagged proteins were purified using a glutathione Sepharose column (GSTrap HP, GE Healthcare), followed by overnight dialysis against A50 buffer containing 1 mM DTT at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and then the proteins were transferred onto the PVDF membrane (GE Healthcare Life Science, 10600023). The blocking and antibody incubation were performed accordingly ...
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bioRxiv - Cell Biology 2024Quote: ... Protein signal was visualized using the Amersham Imager 600 (GE Life Sciences #29-0834-61). The obtained images were quantified using Fiji (imagej.net/software/fiji/) ...