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Citations for GE Life Sciences :
2151 - 2200 of 2374 citations for 4 Triethylsilyl 3 butyn 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... both MSL8N and MSL9N were placed in a high salt storage buffer (1 M NaCl, 200 mM Sodium Phosphate) using gravity PD-10 desalting columns (GE Healthcare). The protein was labeled with Alexa Fluor 647 NHS (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... was diluted to a final salt concentration of 200 mM NaCl before loading onto a 1 mL heparin column (GE Healthcare) equilibrated in low-salt buffer (50 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... was incubated 2 h at a dilution of 1:10,000 and revealed by ECL Prime colorimetric assay (GE Healthcare, Chicago, USA).
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bioRxiv - Biochemistry 2022Quote: ... and passed through two subsequent gel filtration steps using a: 1) Hiload 16/60 Superdex 75 column (Cytiva #28989333) and 2) and Sephacryl S-300 column (GE Healthcare). Buffers used for this sizing step and subsequent labeling reactions were followed as for full-length proteins ...
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bioRxiv - Biochemistry 2022Quote: ... The second PCR joined these two spliced fragments to generate the PCR product encoding the H172Y mutated Mpro including the cleavage sites of the restriction enzymes for cloning into the vector PGEX-6p-1 (GE Healthcare). The amplified PCR product was digested with BamHI and XhoI and ligated into the vector PGEX-6p-1 digested with the same restriction enzymes ...
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bioRxiv - Cell Biology 2022Quote: ... either wild-type (termed GSTscc2PIP) or with PIP mutated (Scc2-N1479A,L1482A,Y1485A,F1486A, termed GSTscc2pip) was introduced into pGEX-6P-1 (GE Healthcare) vector replacing the last 18 residues of GST ...
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bioRxiv - Genetics 2022Quote: ... Products of were separated using 6% denaturing sequencing gel for 1 hr at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... and bands visualized with (HRP)- coupled secondary antibody (1:5,000) (Cell Signalling, rabbit 7074S, mouse 7076S) on an ImageQuant LAS 4000 Mini (GE Life Sciences) with Luminata Forte Western HRP Substrate (Sigma WBLUF0500 ...
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bioRxiv - Molecular Biology 2022Quote: ... validated by genotyping PCR that was analyzed on a 1% agarose gel imaged with a Typhoon FLA 9500 scanner (GE Healthcare). Subsequent Sanger sequencing of the PCR product (Microsynth ...
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bioRxiv - Microbiology 2022Quote: ... of the extension reaction were separated by electrophoresis on a denaturing PAGE (8 M urea, 1× TBE, 15 % acrylamide) and then imaged (Typhoon 9500 imager; GE Healthcare) and the images were quantified using Image Lab software (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Microbiology 2022Quote: ... end-labelled with [γ-32P ATP] (1000 Ci nmol-1) using T4 polynucleotide kinase and purified from free label by Sephadex G-50 spin columns (GE Healthcare). Increasing amounts of purified regulators were incubated with appropriately end-labelled DNA probes in a total volume of 10 µl binding mix (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... after the IMAC column the proteins were diluted to buffer containing 50 mM NaCl and further purified using 1 ml Resource Q cation exchange column (GE Healthcare), where they were eluted with a linear gradient of 50 mM NaCl to 1 M NaCl ...
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bioRxiv - Plant Biology 2024Quote: ... the first cDNA strand was generated from 1 µg of the RNA using Ready-to-Go RT-PCR beads (GE Healthcare) and random oligomers ...
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bioRxiv - Microbiology 2024Quote: ... were injected first onto a His-Trap crude 1 ml column and further purified by FPLC (Äkta purifier-10, GE Healthcare) using a solution of 50 mM Na-Phosphate pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting supernatant was applied to a 10-mL column with 1 mL of Ni-Sepharose (GE Healthcare Life Sciences, 17531801) equilibrated with binding buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with secondary antibodies conjugated to different fluorophores (1:750 dilution) and were imaged in the laser-based InCell high-content imaging system (IN Cell Analyzer 6000, GE Healthcare). The nuclear levels of S9.6 or the number of γ-H2AX foci was quantified using the INCarta software that is attached to the imaging system ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase conjugated antibodies were added at a concentration of 1:7000 in 5% milk in TBST (HRP anti-mouse NA931V; HRP antirabbit NA934V, both GE Healthcare) and detected using chemiluminescence Clarity or Clarity Max ECL western blotting substrate (Biorad 1705061 or 1705062).
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bioRxiv - Microbiology 2024Quote: ... The resulting lysate underwent clarification through centrifugation (125,000g for 30 minutes at 277 K) using a Ti-45 rotor (Beckmann) and was subsequently applied to a 1 ml HisTrap HP column (GE Healthcare). The column underwent an initial wash with five column volumes of lysis buffer containing 40 mM imidazole at pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant clarified from the cell lysate after overnight induction by 0.5mM isopropylthio-β-D-1-galactopyranoside (IPTG) at 20 °C was applied to pre-equilibrated 5Lml HisTrap column (GE Healthcare). After thorough wash with binding buffer (20LmM Tris–HCl pHL7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were obtained 24 hours after treatment and GST was purified on a 1 mL GSTrap HP column (GE Healthcare) with an AKTA purifier 10 FPLC system ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies for Western blotting were HRP-linked sheep anti-mouse IgG and donkey anti-rabbit (both GE Healthcare; 1:5000) and HRP-linked mouse anti-rabbit ...
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bioRxiv - Genomics 2024Quote: ... WSC3-e cells (1.6 µg protein µL-1) was loaded onto a Superdex 200 10/300 column (GE Healthcare, Chicago, USA), which was equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.84) ...
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bioRxiv - Biochemistry 2024Quote: ... Origin substrates were amplified using standard PCR protocols and were purified by anion exchange chromatography using a 1 ml RESOURCE Q column (GE healthcare). Peak fractions were ethanol precipitated and resuspended in TE buffer.
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bioRxiv - Biophysics 2024Quote: ... The filtered supernatant was then incubated at 4 ºC with gentle shaking at 5 rpm for 1 hour in the presence of 5 mL of HisPur Cobalt Resin (GE Healthcare). After multiple rounds of resin cleaning using Extraction Buffer supplemented with 5 mM imidazole ...
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bioRxiv - Neuroscience 2023Quote: ... Reaction products were electrophoretically resolved on 6% denaturing sequencing gel for 1 hour at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare). Nuclease activity quantification compared the densitometric intensity of cleaved versus un-cleaved DNA (ImageQuant ...
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bioRxiv - Cell Biology 2023Quote: ... was applied to a previously equilibrated HiTrap Chelating HP 5 ml column charged with 50 mM CoCl2 and coupled to a GSTrap HP 1 ml column (both GE Healthcare). Buffer A was used for equilibration ...
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bioRxiv - Cell Biology 2023Quote: ... were used at a dilution of 1:10,000 and the HRP-linked sheep F(ab’)2 fragment to mouse IgG (GE Healthcare, NA9310) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... 70 μL of each sample at 1 mg/mL was fractionated in GF150 buffer using a Superdex 200 5/150 analytical gel filtration column (GE Healthcare) run at 0.1 mL/min on an Agilent 1200 series LC system collecting UV at 280 nm before then passing through the light scattering and refractive index detectors in a standard SEC MALS format ...
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bioRxiv - Biochemistry 2023Quote: ... of GcapOCP2 or CrinOCP2 holoform samples (1 mg/ml in 100 μl) was carried out on a Superdex 200 Increase 10/300 column (GE Healthcare) connected to a tandem of consecutive detectors Prostar 335 (Varian ...
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bioRxiv - Genomics 2023Quote: ... The digested DNA fragments were separated by electrophoresis using 1% agarose gels and transferred overnight to Hybond-N+ membranes (GE Healthcare) in 20 × SSC ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Bioengineering 2023Quote: ... A Biosep 10L acoustic filter (Applikon Inc., Foster City, CA) related to the bioreactor thanks to two peristaltic pumps (P-1, GE Healthcare), one for medium recirculation and one for sample withdrawal (Henry et al. ...
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bioRxiv - Cell Biology 2023Quote: ... was used at a dilution of 1:10,000 and the HRP-linked donkey F(ab’)2 fragment to rabbit IgG (GE Healthcare, NA9340) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Biophysics 2023Quote: ... The fixed cells were rinsed three times in warm PBS and incubated for 30 min with a blocking solution containing 1% BSA (GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was stained with DAPI (1 µg/mL) for 5 minutes at room temperature and cells imaged with a DeltaVision Core system (Applied Precision).
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bioRxiv - Neuroscience 2023Quote: ... Blots were washed three times in PBST for 15 min and were then incubated with HRP-conjugated secondary antibodies (1:2,500 dilution; GE Healthcare, NA9340V) for 45 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μl of each sample was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA), and the dry membrane was incubated in NRP solution (0.01 g/ml KMnO4 in ddH2O ...
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bioRxiv - Biophysics 2023Quote: ... The solution was concentrated to ∼1 ml and went through gel filtration on Superdex 75 10/300 increase column (GE Healthcare) equilibrated with a buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... Final purification and concentration of the untagged DAP5 was performed using a 1 ml HiTrap heparin HP column from GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2023Quote: ... As done in our previous experiments (Cattaneo et al., 2022) stimuli aliquots were spread on grade 1 – 20 mm circles filter paper (GE Healthcare Life Science ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3x for 10 min with TBST and subsequently incubated for 1 hr at room temperature with appropriate horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) diluted (1:10,000 ...
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bioRxiv - Plant Biology 2023Quote: ... Vectors suitable for expression and purification of His6-and/or glutathione S-transferase (GST)-tagged recombinant proteins in Escherichia coli were either cloned by Twist Biosciences into the pET28a+ backbone (EMD Biosciences) or cloned in-house into the pGex6p.1 backbone (GE Healthcare). When needed ...
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bioRxiv - Molecular Biology 2023Quote: ... the TEV-cleaved proteins were purified in two steps: 1) IEX on 5 mL HiTrap Q HP (GE Healthcare Life Sciences) using as buffer A ...
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bioRxiv - Microbiology 2023Quote: ... with a 10 kDa cut-off and loaded onto a Superdex 200 pg HiLoad 16/600 (GE Healthcare, 1 mL/min). Sample purity was assessed by 12% SDS-PAGE analysis and sample concentration was determined by spectrophotometry using a theoretical molar extinction coefficient at 280 nm of 20 ...
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bioRxiv - Cell Biology 2023Quote: ... or phosphate buffered saline containing 1% human serum albumin (Grifols Biologicals, Los Angeles, CA, USA) using either a PD10 desalting column (GE Healthcare) or a Vivaflow 50 cassette (Sartorius ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used in immunoblotting: HRP-conjugated donkey anti-rabbit IgG (1:2000) (GE Healthcare, catalog number AB_772206), HRP-conjugated goat anti-mouse IgG (1:5000 ...
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bioRxiv - Systems Biology 2023Quote: ... Isolated cells were washed once in PBS (2mM EDTA, 1% FBS) and lymphocytes were enriched on a 40-67% Percoll gradient (GE Healthcare).