Labshake search
Citations for GE Life Sciences :
2001 - 2050 of 2374 citations for 4 Triethylsilyl 3 butyn 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... at 50°C for 1 hour followed by a light-protected incubation with 55 mM iodoacetamide (GE Healthcare; cat. #RPN6302V) at room temperature for 45 min ...
-
Exploring the druggability of the UEV domain of human TSG101 in search for broad-spectrum antiviralsbioRxiv - Molecular Biology 2024Quote: ... the supernatant was filtered using 0.45 µm syringe filters and loaded onto a 1 mL FPLC-GSTrap column (GE Healthcare) pre-equilibrated in Buffer A at a flow rate of 0.5 mL·min-1 ...
-
bioRxiv - Microbiology 2024Quote: ... The reaction products were electrophoresed on a 23% (10:1, acrylamide:bisacrylamide) polyacrylamide gel with 7 M urea and visualized using the GE Typhoon TRIO Imager (GE Healthcare).
-
bioRxiv - Biochemistry 2024Quote: ... 20 mM imidazole pH 7.5 was added prior to loading solubilized membranes on a pre-equilibrated 1 mL Ni-NTA column (GE Healthcare) at 1 mL min-1 ...
-
bioRxiv - Biochemistry 2024Quote: ... Elution was performed with a gradient of 0.225–1.5 M NaCl in buffer K using Äkta FPLC (gradient length 30 mL, flow rate 1 mL/min, GE Healthcare). CF eluted at ∼825 mM NaCl ...
-
bioRxiv - Biophysics 2024Quote: ... All proteins were purified by Ni-NTA affinity chromatography using a 1 mL His Trap column (GE Healthcare Life Science). The column was equilibrated with balance buffer (50 mM PBS pH 7.5 and 150 mM NaCl ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples at 1-1.5 mg/mL in 250 µL were injected into a Superdex 200 Increase 10/300 GL column (GE Healthcare) at a 0.5 ml/min flow rate ...
-
bioRxiv - Cell Biology 2024Quote: ... Anti-rabbit horseradish peroxidase-linked IgG and anti-mouse IgG, horseradish peroxidase linked antibodies (1:10,000; NA944V and NA931, respectively) were from GE Healthcare.
-
bioRxiv - Microbiology 2020Quote: ... Cells were then washed twice with PBS and incubated for 1 h with anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
-
bioRxiv - Microbiology 2020Quote: ... Purification of the 4D11 mAb was carried out by affinity chromatography using a Protein A/Protein G GraviTrap 1-mL column (GE Healthcare), following the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... by injecting 50 µL of 8 µM protein in Protein Buffer onto a 1 mL HiTrap Heparin HP column (GE Healthcare) and eluting with a 10 CV linear gradient from 150 mM – 1 M NaCl in 20 mM Tris pH 7.5 ...
-
bioRxiv - Biophysics 2021Quote: ... suspended in buffer QA (10 mM Tris pH 8 and 1 mM EDTA) and loaded into a MonoQ 5/50 GL column (GE Healthcare). After a step-wise wash with 10% and 20% of buffer QB (Buffer QA supplemented with 2M NaCl) ...
-
bioRxiv - Biophysics 2021Quote: ... The protein samples without a His tag were purified by an anion-exchange chromatography (HiTrapQ HP 1 mL column, GE healthcare) followed by gel filtration chromatography (Superdex 75 10/300 GL column ...
-
bioRxiv - Biophysics 2021Quote: ... The purification was done by purifying these two supernatants with 1 M NaCl containing Tris buffer using Hi-Trap Q-FF anion exchange column of ÄKTA Pure (GE healthcare). To maintain the oxidation activity of DsbA 0.3% H2O2 was added to the purified protein for overnight ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Immunoglobulins were purified against Protein A from 1 ml of rabbit serum using a Protein A HP SpinTrap (28-9031-32, GE Healthcare) following the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2020Quote: ... the BglII–SalI fragment of HA-Catalase amplified by PCR from pUcD3/HA-HsCatalase (Otera and Fujiki, 2012) was cloned into the BamHI–SalI sites of pGEX6P-1 (GE Healthcare), thereby generating pGEX/HA-HsCatalase ...
-
bioRxiv - Cell Biology 2020Quote: ... The complex was supplied with 2mM GTP and incubated at 4°C for 1 hour prior to purification by size exclusion chromatography on a HiLoad Superdex S75 16/600 column (GE Healthcare) equilibrated in 50mM Tris-HCl pH 8.0 ...
-
bioRxiv - Cell Biology 2020Quote: ... HUVECs were seeded the day before the transfection to reach 60-70% confluence and were then transfected with 25 nM of siRNA using the DharmaFECT 1 reagent (Dharmacon, GE Healthcare) following the Dharmacon siRNA transfection protocol ...
-
bioRxiv - Immunology 2021Quote: ... media was diluted with an equal volume of Buffer A (500 mM NaCl, 20 mM sodium NaH2PO4, 20 mM imidazole) and loaded onto a 1 mL HisTrap column (GE Healthcare). The column was washed with Buffer A and the protein eluted with a gradient to Buffer B (500 mM NaCl ...
-
bioRxiv - Neuroscience 2020Quote: The bacterial expression construct GST-MPP2-SH3-GK was generated by cloning the fragment encoding amino acids 220-552 (SH3GK) of mouse MPP2 into pGEX-6P-1 (GE Healthcare). GST-PSD-95-SH3GK was generated as described before [25] ...
-
bioRxiv - Microbiology 2020Quote: ... ParB-containing fractions were pooled and diluted to a conductivity of 16 mS/cm before being loaded onto a 1-mL Heparin HP column (GE Healthcare) that had been equilibrated with 100 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Developmental Biology 2021Quote: ... which was added to the membrane for 1 min before the signal was detected using the ImageQuantTM LAS 4000 (GE Healthcare). Antibodies and dilutions used ...
-
bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then placed in 5% milk PBST plus 1:2500 rabbit specific horseradish peroxidase (HRP) antibodies (GE Healthcare) at room temperature for 4 hours ...
-
bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
-
bioRxiv - Molecular Biology 2020Quote: ... Purified NanR at 10 mg mL-1 (340 μM) was injected (70 μL) onto an inline Superdex S200 5/150 Increase (GE Healthcare), equilibrated with buffer C ...
-
bioRxiv - Molecular Biology 2020Quote: ... the buffer was supplemented with 30 mM imidazole and an additional 100 mM NaCl and passed through a 1 ml Hi-Trap Chelating column (GE Healthcare) to capture the N-terminal fragment that was cleaved by the TEV protease ...
-
bioRxiv - Biophysics 2022Quote: ... The individual complexes were incubated over ice for 1 hour and purified by size-exclusion chromatography (SEC) using a Superdex 200 10/300 GL column (GE healthcare) pre-equilibrated in a buffer containing 10 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Cell Biology 2022Quote: ... GST-Lc3b was constructed by cloning Lc3b into the BamHI and SalI sites of pGEX-4T-1 (GE Healthcare, IL, USA). The pac sequence was amplified from pBApo-EF1α Pur and cloned into pBApo-EF1α Neo ...
-
bioRxiv - Biochemistry 2020Quote: ... 80 µg of a 5’ amino-modified DNA oligonucleotide complementary to the target tRNA (IDT) was covalently coupled to a HiTrapTM NHS-activated HP column (1 mL, GE Healthcare). For isolation of full-length tRNAs ...
-
bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for 1 hour prior to purification on a Superdex 200 10/300 size-exclusion column (GE Healthcare). Rac1 (G12V ...
-
bioRxiv - Immunology 2020Quote: ... was accomplished using 0.5 M imidazole and fractionated into 1 ml aliquots using the Frac30 fraction collector (GE Life Sciences # 29023051). Fluorescence of FAST proteins was checked via brief excitation (Figure 2D ...
-
bioRxiv - Microbiology 2020Quote: ... followed by anti-mouse IgG horseradish peroxidase (HRP)-linked whole Ab sheep (1:5,000 dilution in PBS) (GE Healthcare Bio-Sciences), and reacted with 3 ...
-
bioRxiv - Biochemistry 2020Quote: ... The reaction was stopped by the addition of 1 mM PMSF and the proteolyzed complex was applied to a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
-
bioRxiv - Genetics 2021Quote: ... Products of were separated using 6% denaturing sequencing gel for 1 hr at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare). Rate of cleavage (nucleotide/minutes ...
-
bioRxiv - Biophysics 2021Quote: ... After dialysis the histones in 2 M NaCl were concentrated to a volume of 1 mL and purified using an Superdex 200 column (GE Healthcare) on an FPLC (Agilent) ...
-
bioRxiv - Microbiology 2021Quote: ... ParBΔCTD-containing fractions were pooled and diluted to a conductivity of 16 mS/cm before being loaded onto a 1-mL Heparin HP column (GE Healthcare) that had been pre-equilibrated with 100 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Molecular Biology 2020Quote: Wild type or ΔN20 hMcm10-ID (200 µL at 5 mg ml-1) was injected into Superdex 75 Increase 10/300 GL column (GE Healthcare) operating with the elution buffer containing 20 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... and EBV BFLF2 residues 78 to 318 (Uniprot entry: K9UT32) were each cloned into a pGEX-6P-1 vector (GE Healthcare). The constructs include an N-terminal GST affinity tag and a HRV 3C protease cleavage site (SDLEVLFQGPLGS) ...
-
bioRxiv - Bioengineering 2021Quote: ... The reaction mixture was incubated at 37°C for 1 h and labeled EVs purified using a PD-10 size exclusion chromatography column (GE Healthcare). Instant thin layer chromatography (iTLC ...
-
bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at room temperature for 1 hour prior to removal of unreacted Biotin-NHS by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... LPMO containing fractions were pooled and concentrated to 1 mL before being loaded onto a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) operated with a running buffer consisting of 50 mM Tris pH 7.5 and 200 mM NaCl ...
-
bioRxiv - Biochemistry 2021Quote: ... The proteins were purified by loading the supernatant onto a 1 mL HisTrap HP Ni-sepharose column (GE Healthcare Life Sciences) equilibrated with 5 CV of 95% Buffer A (50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2021Quote: 50 μl of the N protein samples (1 mg/ml) purified according to protocols 1 or 2 were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...
-
bioRxiv - Biochemistry 2021Quote: ... The flowthrough was diluted 1:5 with MonoQ buffer A and loaded onto a MonoQ 5/50 GL column (GE Healthcare), which was equilibrated with MonoQ buffer A containing 50 mM NaCl ...
-
bioRxiv - Biochemistry 2020Quote: ... primary mouse hepatocytes isolated via the two-step collagenase perfusion method were suspended in 1 ml DMEM media and subjected to Percoll (GE Healthcare) gradient centrifugation at 1,000g for 30 minutes ...
-
bioRxiv - Biochemistry 2020Quote: ... 10 mM MgCl2 1 mM DTT) and further purified by size exclusion chromatography on a pre-equilibrated Superdex 200 column (GE healthcare). Protein in size exclusion buffer was concentrated to ∼10 mg/mL ...
-
bioRxiv - Biochemistry 2020Quote: ... The eluate was diluted two-fold in buffer E + 1 mM DTT + 150 mM NaCl and applied to a MonoS column (GE Healthcare) pre-equilibrated in buffer E + 150 mM NaCl + 1 mM DTT + 0.5 mM EDTA ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated to less than 1 mL and was injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES pH 7.7 ...
-
Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 293-F cells were seeded 2-3 h before transfection at a density of 1×106 cells per ml in Freestyle medium supplemented with 0.2% w/v HyClone Cell Boost (GE Healthcare, SH30584.02). For transient transfection ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cleaved KIX was purified using a linear gradient of 50 mM to 1 M NaCl over ten column volumes by cation exchange chromatography using MonoS 5/50 GL column at a flow rate of 1 mL per minute (GE Healthcare). Fractions containing purified KIX were confirmed by SDS-PAGE and dialyzed against 1 L of 50 mM potassium phosphate pH 5.5 ...