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Citations for GE Life Sciences :
2101 - 2150 of 5411 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was centrifuged for 10 minutes at 25,000g and the lysate supernatant was incubated for 3 hours with 1 mL of glutathione sepharose beads (GE Healthcare) pre-washed in lysis buffer ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were separated into 24 fractions using the 24 cm IPG strips pH 3-10 NL (GE HealthCare, Chicago, IL). To achieve the required low salt concentration 2.5 mg wheat germ extract was diluted with water and centrifuged 10 min. ...
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bioRxiv - Biochemistry 2019Quote: ... Wet gels were exposed to phosphor screen (0.5–3 hrs) before being scanned using a Typhoon FLA 7000 imaging system (GE Healthcare). Background subtracted autoradiograph band intensities were determined with ImageStudio (Li-COR ...
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bioRxiv - Biophysics 2020Quote: ... A volume of 50 μl of each sample at 3 mg·ml−1 was loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 25 mM Hepes pH 7.8 ...
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bioRxiv - Immunology 2020Quote: ... with a 3 kDa membrane cutoff and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare Life Sciences) size exclusion chromatography column previously equilibrated with 7M urea ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Physiology 2022Quote: ... Membranes were then washed 3 more times and the signal was detected by chemiluminescence using ECL Prime reagent (GE Healthcare) on an Amersham Imager 600 detection system (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... and washed 10 minutes 3 times in PBST before being incubated with horseradish peroxidase-conjugated anti-rabbit IgG (GE Healthcare) diluted 1:2000 in the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... or with horseradish peroxidase (HRP)-conjugated secondary antibodies followed by a 3-min incubation with a chemiluminescent substrate (GE Healthcare) for ChemiDoc imaging ...
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bioRxiv - Bioengineering 2023Quote: ... All images were acquired in a 3 Tesla MRI with 32 channel receiver system (GE Healthcare, Chicago, Illinois 60661, USA) and made anonymous in compliance with HIPAA standards and rules ...
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bioRxiv - Cell Biology 2022Quote: ... 6 µL of the reaction were diluted to 100 µL in 2x SSC (3 M NaCl, 0.3 M sodium citrate) and slot-blotted on a Hybond XL nylon membrane (GE Healthcare). For detection of ChIP samples ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Neuroscience 2023Quote: The experiments were conducted using a 3-Tesla scanner equipped with an 8-ch phased array coil (GE Healthcare, Signa). Functional images were acquired using a T2*-weighted gradient-echo echo-planar imaging sequence ...
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bioRxiv - Immunology 2024Quote: ... membranes were finally incubated for 3 min with an enhanced chemiluminescence assay (ECL-Kit, GE Healthcare Life Science, Amersham, UK) and digital images of the responsive bands automatically captured via an imaging system (ChemiDoc MP ...
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bioRxiv - Biophysics 2021Quote: ... lysed protein was purified by cation exchange using a 1 mL HiTrap SP HP (GE Healthcare) in protein buffer (50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biophysics 2021Quote: ... The protein was purified from the lysate using Ni2+-NTA column of [KTA Pure (GE healthcare). For in vitro biotinylation of the polyprotein ...
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bioRxiv - Biophysics 2020Quote: ... the protein was loaded onto a HiLoadTM 26/10 Q Sepharose high performance column (GE Healthcare), and eluted at ∼350 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... the proteins were purified by chromatography on a HiLoad 16/60 Superdex 200 column (GE Healthcare), in buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were blotted onto a nitrocellulose membrane (Amersham Protran 0.2 NC, GE Healthcare life sciences) for 1-2h at 300 mA ...
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bioRxiv - Cell Biology 2020Quote: ... The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences). The beads were washed with binding buffer at least four times ...
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bioRxiv - Cell Biology 2020Quote: ... Further purification of S-nitrosylated proteins were done using Thiopropyl Sepharose 6B bead (GE Healthcare# 170420001) in the presence of 50mM Na-ascorbate as a mild reducing agent ...
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bioRxiv - Immunology 2021Quote: ... Protein bands were visualised using Amersham™ Imager 600 (GE Healthcare Life Sciences, Malborough, MA, USA).
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bioRxiv - Microbiology 2021Quote: ... Protein-containing fractions were pooled for desalting through a 5 mL HiTrap Desalting column (GE Healthcare). Desalted protein was dialyzed into a storage buffer (50 mM NaH2PO4 pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant protein was purified by chromatography using a Nickel-loaded Hitrap Chelating HP column (GE Healthcare). Unbound material was extensively washed using Tris 20mM pH8 ...
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bioRxiv - Developmental Biology 2020Quote: ... and GST-tagged target protein was purified using Glutathione Sepharose 4B (GE Healthcare, Marlborough, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Immuno-detected proteins were visualized by chemiluminescent photographic film exposure (ECL kit; GE Life Sciences, RPN2232) and digitally scanned using a GS-800 calibrated densitometer (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was then purified by ion exchange chromatography (mono Q 10/100 GL, GE Healthcare), followed by SEC using a Superdex 200 increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) with PBS as the running buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) with PBS as the running buffer ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-fusion proteins were coupled to Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 2 h at 4 °C with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was subjected to size-exclusion chromatography on a HighLoad 16/60 Superdex75 (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... protein-RNA complexes were immobilized on Protran nitrocellulose membrane (0.45 μm pore size, Whatman, GE Healthcare) and unbound RNA immobilized on Hybond Nylon membrane (Amersham ...
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bioRxiv - Molecular Biology 2020Quote: ... 1500 µg protein extract containing GST-Gal1p was loaded on a GST SpinTrap column (GE Healthcare) and incubated for 1.5 hour at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... Fc fragments and undigested IgG were removed from binding to the protein A beads (GE Healthcare). CC12.1 and eCC12.1.7 Fab fragments were generated by introducing a stop codon after the CH1 region in heavy chain expression plasmids ...
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bioRxiv - Biophysics 2022Quote: ... The protein was further purified by anion-exchange chromatography on a Mono-Q column (GE healthcare). Expression and purification of RT600 was carried out in a similar way as with RT139A except that the 6x-His tag on p51 N-terminus was not cleaved ...
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bioRxiv - Biophysics 2022Quote: ... Protein was then affinity purified from lysate using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer (100 mM tris-HCl ...
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bioRxiv - Cancer Biology 2019Quote: ... A suspension of Optison microbubbles (Perflutren Protein-Type A Microspheres Injectable Suspension, USP, GE Healthcare, USA) was infused intravenously (15 μl/minute ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: ... Eluted protein was concentrated and subjected to a HiLoad 16/600 Superdex 75 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... protein supernatant was bound to a phenyl sepharose column (High Load FF 16/10, GE Healthcare). The column was washed with buffer C and protein eluted with 30% ethanol in water ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was fractionated on a HiLoad 16/600 Superdex 200 gel filtration column (GE Healthcare), eluting with SEC buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein A Sepharose 4 Fast Flow and Con A Sepharose 4B were purchased from GE Healthcare.
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bioRxiv - Molecular Biology 2019Quote: ... the reaction was added to 25 µL (50 µL 50% slurry) Protein G beads (GE Healthcare) pre-coupled with antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... and C-terminal 6xHis-tagged effector proteins were immobilised onto a NTA sensor chip (GE Healthcare). HMA protein was flowed over the immobilised effector at 30 µl/min (360 sec contact time and 180 sec dissociation time ...
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bioRxiv - Molecular Biology 2019Quote: ... the protein was further purified through HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare) and eluted in buffer containing 20 mM Tris-Cl (pH 7.5) ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... vector and GST-tagged or MBP-tagged proteins were captured using Glutathione Sepharose beads (GE Healthcare) or amylose resin (New England Biolabs) ...
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bioRxiv - Immunology 2019Quote: ... The expected protein bands were detected using Image Quant LAS 4000 (GE Healthcare Life Sciences, USA). The relative abundance of the target protein (normalized to β-actin ...
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bioRxiv - Microbiology 2019Quote: ... Buffer exchange of the purified protein was performed by using PD-10 desalting column (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by gel filtration in Superdex 75 pg 16/600 (GE Healthcare) in 25 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by gel filtration in Superdex 75 pg 16/600 (GE Healthcare) in 25 mM Tris ...