Labshake search
Citations for GE Life Sciences :
2351 - 2400 of 5411 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... Recombinant IgG was harvested and purified after 6 days with protein G sepharose beads (GE Healthcare), dialyzed in PBS ...
-
bioRxiv - Immunology 2023Quote: ... The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe, GE Healthcare), followed by a preparative size exclusion chromatography (SEC) ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein-containing fractions were pooled and further purified on a Superdex 200 column (GE Healthcare), equilibrated ...
-
bioRxiv - Developmental Biology 2023Quote: ... The proteins were then transferred from the gel to the PVDF membrane (GE Healthcare Life-Science) in Bio-Rad vertical gel Transfer Apparatus using Transfer buffer (2.5mM Tris base ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Recombinant proteins were then purified by 1 mL Ni Sepharose (17-5318-01, GE Healthcare, USA). Columns were sequentially washed with 20 mL buffer 1 and 10 mL buffer 2 (in mM ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Biotinylated proteins were purified using SEC on a Superdex 200 10/300 Increase GL (GE Healthcare) or S75 10/300 Increase GL (GE Healthcare ...
-
bioRxiv - Biochemistry 2023Quote: ... The proteins were further purified by gel filtration on Superdex 75 pg 16/600 (GE Healthcare) in 25 mM Tris-HCl ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein detection was finally performed using Amersham ECL Western Blotting detection reagent (GE Healthcare #CA95038-564L).
-
bioRxiv - Immunology 2023Quote: ... The proteins were visualized by ECL-Plus (GEHealthcare) using Image Quant LAS500 (GE Healthcare Life Sciences). The following antibodies were used ...
-
bioRxiv - Plant Biology 2023Quote: Protein samples were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). After being blocked in 1 × PBST buffer containing 5% skimmed milk ...
-
bioRxiv - Plant Biology 2023Quote: ... The protein concentration was determined with 2-D Quant Kit (GE Healthcare Life Sciences, Westborough, USA) using Bovine Serum Albumin (BSA ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biophysics 2023Quote: ... Soluble protein was maintained at 4°C and purified using Ni-NTA affinity resin (GE Healthcare). A column wash was performed using pH 7.0 HisA containing 300 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... 25µL of Protein L beads (Cat. no. Capto™ L resin, GE Healthcare Cat. no. 17547802), pre-equilibrated with binding buffer (20mM HEPES ...
-
bioRxiv - Molecular Biology 2023Quote: ... 400 μL of 10% (v/v) protein A Sepharose CL-4B beads (GE Healthcare Life Sciences) in IP-2 buffer [50 Mm Tris pH 7.9 ...
-
bioRxiv - Biochemistry 2023Quote: ... Separation of the labeled protein from residual dye was carried out using a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer ...
-
bioRxiv - Immunology 2023Quote: ... the protein was further purified by size-exclusion chromatography on a Sephacryl 300 column (GE Healthcare) in PBS.
-
bioRxiv - Bioengineering 2023Quote: ... Affinity purification of the protein was achieved using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer consisting of 100 mM tris-HCl ...
-
bioRxiv - Molecular Biology 2023Quote: ... A small portion of the final proteins were run through the Superdex 200 columns (GE Healthcare) again for quality check.
-
bioRxiv - Genomics 2023Quote: ... 167 mM NaCl) and pre-cleared with protein-A-Sepharose beads (GE Healthcare 17-5280-02) for 30 min at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... Samples were pre-cleared with 40 µl of protein G Sepharose beads (50% slurry, GE Healthcare) plus 5 μg Goat IgG (Invitrogen) ...
-
bioRxiv - Cell Biology 2023Quote: ... and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare) were performed according to our published procedure (Ninagawa et al. ...
-
bioRxiv - Biophysics 2023Quote: ... The protein was injected onto a Superdex-75 Increase 10/300 GL column (GE Healthcare 29148721) for size exclusion chromatography in SEC buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Free copper was then removed by passing the protein through a HiTrap Desalting column (GE Healthcare). Prior to the SANS experiments ...
-
bioRxiv - Biophysics 2023Quote: ... The protein was further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2023Quote: ... The protein was loaded onto a HiLoad™ 16/600 Superdex™ 200 column (GE Healthcare) equilibrated with a buffer containing 30 mM Tris-HCl pH7.5 ...
-
bioRxiv - Biochemistry 2023Quote: Anti-ASCT2 Ab was purified from the anti-sera using HiTrap Protein G HP (GE Healthcare) following the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2023Quote: ... All proteins were further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Cell Biology 2024Quote: ... GST or the GST fusion protein was purified using a glutathione Sepharose 4B column (GE Healthcare). GFP::RAB-5 protein from wild-type or usp-50(xd413 ...
-
bioRxiv - Biochemistry 2023Quote: ... and flowthrough containing phosphorylated proteins were purified over Hiload 16/600 Superdex 75pg column (GE Healthcare) pre-equilibrated with buffer containing 25mM Tris pH 7.5 ...
-
bioRxiv - Cell Biology 2024Quote: ... the proteins of interest were revealed by chemiluminescence using the ECL WesternBlot detector kit (GE Healthcare) and the results were digitised in a UVITEC11Photo Imager (Ubitec Photoimager ...
-
bioRxiv - Microbiology 2023Quote: ... The eluted protein was concentrated and applied to a Superdex increase S200 10/300 (GE Healthcare) size exclusion column equilibrated with 25 mM HEPES-NaOH ...
-
bioRxiv - Microbiology 2024Quote: ... and the surface proteins were bound to streptavidin Sepharose beads overnight at 4°C (GE Healthcare). Beads were then washed with buffer containing 100 mM Tris ...
-
bioRxiv - Microbiology 2024Quote: ... and the proteins were then transferred onto nitrocellulose blotting membranes (NC) (10600001; GE Healthcare Life Science). The membranes were blocked with 5% non-fat dry milk and 0.1% Tween 20 for 30 min at 37°C ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and the supernatant was collected for protein purification with Ni-NTA columns (GE Healthcare, Uppsala, Sweden). The proteins were dialyzed with PBS at 4 °C and concentrated.
-
bioRxiv - Developmental Biology 2024Quote: ... The overexpressed proteins were bound to the washed Ni2+-chelating Sepharose (GE Healthcare, Pittsburgh, PA, USA). The suspension was mixed on a three-dimensional rotating mixer for 40 min at 4 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant was fitered and protein purification was conducted using a Resource Q column (GE Healthcare) equilibrated in Buffer A ...
-
bioRxiv - Synthetic Biology 2023Quote: ... All proteins were further purified with Superdex 200 Increase 10/300 GL (GE Healthcare Life Sciences) size-exclusion chromatography (SEC ...
-
bioRxiv - Cell Biology 2024Quote: ... Labelled protein bands were detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, USA) and ChemiDoc MP Imaging System (Bio-Rad ...
-
bioRxiv - Biophysics 2024Quote: ... the protein was purified by size exclusion chromatography using a Sephadex 200 16/60 (GE Healthcare) column with the lysis buffer.
-
bioRxiv - Biophysics 2024Quote: ... The protein sample was passed through His Trap™ FF crude column (GE Healthcare, Piscataway, NJ). We expected the undigested protein to remain bound to the column ...
-
bioRxiv - Biophysics 2024Quote: ... A calibration curve was produced for eluting species using known protein standards (GE Healthcare Life Sciences). The dimerisation-impaired D123P construct of Ire1-LD was used to assign the apparent molecular weight of eluted monomeric protein ...
-
bioRxiv - Biochemistry 2024Quote: ... The free dye was separated from labelled protein by a Hi-TRAP Desalting column (GE Healthcare). The final degree of labelling of FtsZ and ZapD was calculated from the molar absorption coefficients of the protein and the dye ...
-
bioRxiv - Genetics 2024Quote: ... The proteins were electrophoresed on 10% SDS□PAGE gels and transferred to nitrocellulose membranes (GE Healthcare). The blots were blocked in 5% milk and incubated with primary antibodies overnight at 4 °C ...
-
Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... proteins were electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 90 V for 90 min on ice in transfer buffer (48 mM Tris and 39 mM glycine supplemented with 20% (v/v ...
-
bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
-
bioRxiv - Cell Biology 2020Quote: ... were incubated with GST or GST-tagged angulin-1 cytoplasmic region (409-575 aa or 409-570 aa) for 2-3 h at 4°C and further incubated with Glutathione Sepharose 4B beads (GE Healthcare) for 1 h at 4°C ...
-
bioRxiv - Microbiology 2021Quote: 30 ml PBS flow-through plasmapheresis filters or peripheral blood diluted in PBS (1:3) were collected in 50 ml tubes and underlaid with Ficoll-Paque (GE Healthcare). PBMC-containing buffy coat was obtained after centrifugation for 30 min at room temperature at 1200 RCF ...
-
bioRxiv - Biophysics 2022Quote: ... the volume was reduced to a volume of 400 ml using the ÄktaFlux system with a 3 kDa MWCO cartridge (GE Healthcare). Afterwards the system was used for buffer exchange with 50 mM HEPES ...