Labshake search
Citations for GE Life Sciences :
2101 - 2150 of 2675 citations for 8 CHLORO 2 METHYL IMIDAZO 1 2 A PYRIDINE 3 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant for each nanobody was then applied to one 1 ml Ni-NTA pre-packed column (GE healthcare) pre-equilibrated with base buffer + 30 mM imidazole ...
-
bioRxiv - Biochemistry 2024Quote: ... using 10/12% acrylamide/bisacrylamide (29:1) 0.1% SDS gels and transferred to a 0.2 μm PVDF Hybond-P membranes (Amersham/GE healthcare) at 20V for 1h in a semi-dry Trans-Blot SD cell system (Bio-Rad ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 ml protein was loaded onto the analytical size exclusion column Superdex 200 increase 10/300 GL (GE Healthcare) in running buffer (25 mM Tris-HCl ...
-
bioRxiv - Microbiology 2024Quote: ... was used in combination with a secondary anti-mouse antibody conjugated to horseradish peroxidase (GE Healthcare NXA931, 1:5000). Primary rabbit anti-mCherry antibody (BioVision 5993 1:2000 ...
-
bioRxiv - Genomics 2024Quote: Ten μl of PCR product were then mixed with 1 μl of Streptavidin sepharose™ high performance (GE Healthcare) and 40 μl of PyroMark binding buffer (Qiagen) ...
-
bioRxiv - Plant Biology 2020Quote: ... 1 mM EDTA) affinity chromatography followed by size-exclusion chromatography on a HiLoad 16/600 Superdex 200pg column (GE Healthcare), equilibrated in 20 mM sodium citrate pH 5.0 ...
-
bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
-
bioRxiv - Biophysics 2021Quote: ... Cell lysates were centrifuged at 75,000 x g for 25 min and supernatants were loaded on a GSTrap FF 1 mL column (GE Healthcare) using an AKTA Start purification system (GE Healthcare ...
-
bioRxiv - Cancer Biology 2021Quote: ... The supernatant was then supplemented with 5 mM imidazole prior to loading onto a 1-ml HisTrap column (GE Healthcare) attached to an AKTA system at 1 ml/min ...
-
bioRxiv - Biochemistry 2021Quote: ... GST-PTP1B was purified with a 1 ml GSTrap FF Column on an ÄKTA pure purification system (GE Healthcare GmbH.) using a binary buffer system (buffer A ...
-
bioRxiv - Cell Biology 2019Quote: ... Buffer was then exchanged to 1xSAB supplemented with 1 mM ATP to remove imidazole using PD-25 column (GE Healthcare).
-
bioRxiv - Cell Biology 2020Quote: ... Cell Signalling) and horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (respectively NA931V and NA934V. 1:10.000. GE Healthcare). Mineral dissolution activity of Oc was measured as described (Maurin et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... 2004) primary antibody and horseradish peroxidase linked anti-rabbit IgG secondary antibody (1:5000, from donkey, GE Healthcare Life Sciences). Signal was detected by chemiluminescence (Luminata Forte Western HRP substrate ...
-
bioRxiv - Cell Biology 2020Quote: Cells lysates were prepared using 1 % triton lysis buffer and precleared with prewashed Protein G Sepharose (PGS) beads (GE Healthcare) for 30 min on a shaker at 4 °C ...
-
bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
-
bioRxiv - Cell Biology 2020Quote: ... The membranes were then probed with HRP-conjugated anti-mouse IgG (Jackson Immunochemicals; 1:10,000) and binding was detected using ECL (GE Healthcare) in a LAS4000 instrument.
-
bioRxiv - Immunology 2021Quote: ... The diluted eluate was then loaded onto a 1 ml HiTrap Heparin HP column (GE Healthcare, Chalfont St Giles, UK). After loading ...
-
bioRxiv - Genomics 2020Quote: ... The membrane was incubated with 1:10,000 dilution of anti-rabbit IgG conjugated to horse radish peroxidase (HRP; GE Healthcare) diluted in 2.5% skimmed milk in TBS-T or anti-mouse IgG conjugated to HRP (GE Healthcare ...
-
bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed 4 times in TBS-T and then incubated with secondary antibodies for 1h (1:4,000; GE Healthcare) and washed 3 times with TBS-T ...
-
bioRxiv - Physiology 2022Quote: ... Supernatants were collected after centrifugation at 13,200 rpm for 15 min and precleaned for 1 h with 50% protein G-Sepharose (GE Healthcare), followed by incubation with an indicated antibody at 4 °C Upon addition of 100 μl of 50% protein G-Sepharose ...
-
bioRxiv - Molecular Biology 2021Quote: ... pH 3.0 (pH neutralization to 7.8 with 1 M Tris-HCl, pH 9.0) using ÄKTA FPLC system (GE Healthcare Life Sciences). The HE-Fc containing fractions were dialyzed in PBS and concentrated using 30kD Amicon Ultra-15 filters (EMD Millipore) ...
-
bioRxiv - Molecular Biology 2020Quote: ... were used at 1:10,000 and blots developed with ChemiGlow detection reagent and visualized using ImageQuant LAS 4000 chemiluminescent camera (GE Healthcare).
-
bioRxiv - Cell Biology 2022Quote: ... These were incubated for 1 hr at room temperature and blots were developed using Enhanced Chemiluminescence (ECL prime, GE healthcare). Chemiluminescence signals were detected by a LAS 4000 (GE healthcare ...
-
bioRxiv - Genomics 2020Quote: ... Mouse Anti-Rabbit IgG (Light Chain Specific) (CST, #93702S) (1:5000) or Rabbit IgG HRP Linked Whole (GE Healthcare, NA9340), respectively ...
-
bioRxiv - Cell Biology 2019Quote: ... adjusted to 100 mM Tris-HCl pH 8.0 and 1 mM EDTA and loaded onto a StrepTrap HP column (GE Healthcare) pre-equilibrated in 100 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Genetics 2019Quote: ... we used the anti-rabbit HRP-conjugated antibody (1/5000 dilutions) with the ECL Western Blotting Detection System (GE Healthcare) according to the manufacturer’s protocol.
-
bioRxiv - Synthetic Biology 2020Quote: ... the pH adjusted to 5.0 with 1 M HCl and loaded on a Mono S column HR10/30 (GE Healthcare), equilibrated with 25 mM acetate buffer ...
-
bioRxiv - Microbiology 2020Quote: ... The lysate was centrifuged for 1 h at 95,000g and the supernatant was loaded onto a Glutathione Sepharose HP (GE Healthcare) column ...
-
bioRxiv - Cell Biology 2019Quote: ... The extract was pre-cleared by centrifugation at 20.000 x g for 45 minutes and the supernatant was incubated for 1 hr with Streptactin Sepharose beads (GE Healthcare) which had been washed 3 times in the lysis buffer ...
-
bioRxiv - Biophysics 2019Quote: ... the modified f(Ab)1 fragment was purified and separated from unbound dye through a gel filtration column (GE Healthcare illustra NAP Columns from Fisher ...
-
bioRxiv - Microbiology 2021Quote: ... Cell debris was removed by centrifugation at 35,000g for 20 min and the supernatant from 1 l of cell culture was incubated with 2.5 ml of Glutathione Sepharose beads (GE Healthcare) at 4°C for 6 h The Glutathione Sepharose beads were then collected in a PD10 column and washed with 50 ml of PBS ...
-
bioRxiv - Microbiology 2021Quote: ... The membrane was washed in 0.1% SDS/0.1X SSC and hybridized DNA was visualized and quantitated by phosphor-imaging on a Typhoon Scanner (GE Healthcare). The 32P-radiolabeled probe was generated from a plasmid containing the entire HPV16 genome by radiolabeling using a Random Prime labeling kit (Roche).
-
bioRxiv - Microbiology 2020Quote: ... non-fat dry milk in TBST for 1 hr at room temperature: anti-rabbit or anti-mouse IgG-HRP conjugated antibody from sheep (both 1:10,000 GE Healthcare). Proteins were visualized using ECL or SuperSignal West Femto chemiluminescence reagents (Pierce ...
-
bioRxiv - Biophysics 2020Quote: ... which is complementary to the tracrRNA 5’-end was labeled with ATTO647N-NHS (1:60 DNA:dye molar ratio) and purified using G-25 micro spin column (Illustra, GE Healthcare). The measured labeling efficiency of ATTO647N-oligonucleotide was >80% ...
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant was loaded onto a 1-ml HisTrap column connected to an ÄKTA Explorer 100 purification system (GE Healthcare), previously equilibrated with Buffer A ...
-
bioRxiv - Biochemistry 2020Quote: ... The resulting eluate was manually applied at room temperature to a 1 mL HiTrapQ anion exchange column (GE Healthcare # 29051325) that had been pre-equilibrated in buffer B ...
-
bioRxiv - Neuroscience 2020Quote: ... for 1 hour at 30V onto Amersham Hybond P 0.45 PVDF membranes (GE Life Sciences, Pittsburgh, PA; cat # GE 10600029). After transfer membranes were rinsed in TBST and blocked for one hour in either 5% milk (PERK ...
-
bioRxiv - Neuroscience 2020Quote: ... Membranes were washed with TBST three times and incubated with sheep anti-mouse or donkey anti-rabbit IgG conjugated with horseradish peroxidase (HRP) (1:4,000, GE Healthcare) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... The supernatant was incubated at 4°C for 1 h with Ni-charged IMAC Sepharose 6 Fast Flow (GE Healthcare). The resin was then washed three times with His-A buffer (lysis buffer + 20 mM imidazole) ...
-
bioRxiv - Biochemistry 2021Quote: ... and 0.1% FA (Fluka) and crosslinked peptides were enriched by separation on a Superdex Peptide PC 3.2/30 column (GE Healthcare), using an Agilent 1100 chromatography system at 95 μL/minute with fraction collection ...
-
bioRxiv - Cell Biology 2021Quote: ... The reaction was quenched with 1 μl 14.3 M βME followed by final buffer exchange using size exclusion chromatography (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
-
bioRxiv - Plant Biology 2020Quote: ... The supernatant was filtered through tandem 1-μm and 0.45-μm filters before Ni2+-affinity purification (HisTrap excel, GE Healthcare). Ni2+-bound proteins were washed with buffer A and eluted directly onto a coupled Strep-Tactin Superflow XT column (IBA ...
-
bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with the secondary antibody (HRP-conjugated anti-mouse or anti-rabbit IgG antibody, GE Healthcare; 1:2000) in TBST plus 2% BSA ...
-
bioRxiv - Microbiology 2020Quote: ... and eluted in superdex buffer (20 mM Tris [pH 8.0], 300 mM NaCl, 1 mM TCEP) using the AKTA Explorer FPLC system (GE Healthcare). The protein was concentrated to 7.5 mg/ml.
-
bioRxiv - Plant Biology 2021Quote: ... α-P4 or α-P6 before subsequent addition of secondary horseradish peroxidase (HPR)-conjugated antibodies (1:20.000; Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
-
bioRxiv - Microbiology 2021Quote: ... the obtained supernatants were filtered through sterile PVDF filters (pore size 0.22 μm) and loaded on 1 ml His-Trap columns (GE Healthcare), pre-equilibrated with ten column volumes of each Millipore water and sodium phosphate buffer A ...
-
bioRxiv - Microbiology 2020Quote: ... The clarified lysate was then passed using an ÄKTA Purifier FPLC through a 1 ml HisTrap HP column (GE Healthcare), pre-equilibrated in lysis buffer ...
-
bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG secondary antibodies (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... with a JA30.50 Ti rotor at 20,000 ×g and applied to a HiTrap SP HP column (GE Healthcare; 1 ml) equilibrated in buffer A (20 mM Na-phosphate buffer (pH 7.0) ...