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Citations for GE Life Sciences :
2051 - 2100 of 2537 citations for Progesterone ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The BRCA1RING/BARD1RING and BRCA1RING/BARD1193 constructs were concentrated and further purified using a 120 mL Superdex 75 column on an AKTA FPLC system in SEC buffer (25 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 1 mM DTT; GE Healthcare). For the BRCA1RING/BARD1216 ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with specific peroxidase-conjugated secondary antibodies [sheep anti-mouse IgG HRP-linked (1:1000; NA931, GE Healthcare) and donkey anti-rabbit IgG HRP-linked (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Biochemistry 2023Quote: Protein samples within the concentration range 0.5-1 mg/mL were injected onto pre-equilibrated Superose 6 10/300 size-exclusion chromatography column (GE Healthcare) in buffer containing 20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were incubated for 1 h with horseradish peroxidase (HRP)-conjugated secondary antibody (dilution 1:10,000) and developed with enhanced chemiluminescence (ECL) reagent (GE Healthcare). All western blots were visualized with a LAS4000 Imaging Station (GE Healthcare) ...
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bioRxiv - Plant Biology 2024Quote: ... NATA2Δ) as well as the full-length NATA2 from Arabidopsis were cloned to the pGEX6P-1 expression vector (GE Healthcare). The plasmids were then transformed into Rosetta 2 (DE3 ...
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bioRxiv - Cell Biology 2023Quote: ... The column was run at a flow rate of 0.5 mL min-1 on an AKTAbasic liquid chromatography system (GE Healthcare) coupled to a miniDawn MALS detector and an Optilab refractive index detector (Wyatt Technology) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to Amersham™ Protran® Western blotting membranes, nitrocellulose (pore size 0.2 μm, roll W × L 300 mm × 4 m, pkg of 1 ea) (GE healthcare) overnight 4 °C at 15 V ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in the lysates was determined by Bradford assay against BSA and 1-3mg protein-lysate equivalent was loaded onto 10μL of bead slurry (Dynabeads, GE healthcare) prewashed twice with lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... the independently purified SMC1ACC-EQ/RAD21C and SMC3CC-EQ/RAD21N samples were mixed with a 1:0.9 ratio and loaded onto a Superdex 200 16/600 column (GE Healthcare) equilibrated with the purification buffer (200 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... The amplified PCR product was digested with BamHI and XhoI and ligated into the vector PGEx-6p-1 (GE Healthcare) digested with the same restriction enzymes ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was quenched with 1 μl 14.3 M βME followed by final buffer exchange using size exclusion chromatography (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... the supernatant was filtered (0.45 polyvinylidene fluoride membrane, Carl Roth, Germany) and applied onto a 1 ml Nickel-IDA column (GE Healthcare) equilibrated in 50 mM TRIS-HCl pH 7.8 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed twice with PBS and incubated for 1 h with anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the 4D11 mAb was carried out by affinity chromatography using a Protein A/Protein G GraviTrap 1-mL column (GE Healthcare), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... by injecting 50 µL of 8 µM protein in Protein Buffer onto a 1 mL HiTrap Heparin HP column (GE Healthcare) and eluting with a 10 CV linear gradient from 150 mM – 1 M NaCl in 20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... suspended in buffer QA (10 mM Tris pH 8 and 1 mM EDTA) and loaded into a MonoQ 5/50 GL column (GE Healthcare). After a step-wise wash with 10% and 20% of buffer QB (Buffer QA supplemented with 2M NaCl) ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples without a His tag were purified by an anion-exchange chromatography (HiTrapQ HP 1 mL column, GE healthcare) followed by gel filtration chromatography (Superdex 75 10/300 GL column ...
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bioRxiv - Biophysics 2021Quote: ... The purification was done by purifying these two supernatants with 1 M NaCl containing Tris buffer using Hi-Trap Q-FF anion exchange column of ÄKTA Pure (GE healthcare). To maintain the oxidation activity of DsbA 0.3% H2O2 was added to the purified protein for overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified by centrifugation (15000 rpm for 1 h at 4 °C) and incubated with glutathione Sepharose 4B (GE Healthcare) for 4 h at 4 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Immunoglobulins were purified against Protein A from 1 ml of rabbit serum using a Protein A HP SpinTrap (28-9031-32, GE Healthcare) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... the BglII–SalI fragment of HA-Catalase amplified by PCR from pUcD3/HA-HsCatalase (Otera and Fujiki, 2012) was cloned into the BamHI–SalI sites of pGEX6P-1 (GE Healthcare), thereby generating pGEX/HA-HsCatalase ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody-antigen complexes were recovered by incubating for 1 h at 4°C with Protein A-Sepharose CL-4B (GE Healthcare) and eluted with Laemmli sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The complex was supplied with 2mM GTP and incubated at 4°C for 1 hour prior to purification by size exclusion chromatography on a HiLoad Superdex S75 16/600 column (GE Healthcare) equilibrated in 50mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... HUVECs were seeded the day before the transfection to reach 60-70% confluence and were then transfected with 25 nM of siRNA using the DharmaFECT 1 reagent (Dharmacon, GE Healthcare) following the Dharmacon siRNA transfection protocol ...
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bioRxiv - Immunology 2021Quote: ... media was diluted with an equal volume of Buffer A (500 mM NaCl, 20 mM sodium NaH2PO4, 20 mM imidazole) and loaded onto a 1 mL HisTrap column (GE Healthcare). The column was washed with Buffer A and the protein eluted with a gradient to Buffer B (500 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: The bacterial expression construct GST-MPP2-SH3-GK was generated by cloning the fragment encoding amino acids 220-552 (SH3GK) of mouse MPP2 into pGEX-6P-1 (GE Healthcare). GST-PSD-95-SH3GK was generated as described before [25] ...
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bioRxiv - Microbiology 2019Quote: RNA free genomic DNA samples were diluted to the desired concentration and 1 µl of each sample was spotted on Amersham Hybond-N+ membranes (GE Healthcare). For anti-5mC blots ...
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bioRxiv - Microbiology 2021Quote: 30 ml PBS flow-through plasmapheresis filters or peripheral blood diluted in PBS (1:3) were collected in 50 ml tubes and underlaid with Ficoll-Paque (GE Healthcare). PBMC-containing buffy coat was obtained after centrifugation for 30 min at room temperature at 1200 RCF ...
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bioRxiv - Microbiology 2020Quote: ... ParB-containing fractions were pooled and diluted to a conductivity of 16 mS/cm before being loaded onto a 1-mL Heparin HP column (GE Healthcare) that had been equilibrated with 100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... and chromatin was released from the resin with 20 µl of ChIP buffer 1 supplemented with 100 µg/ml BSA and 4 µg HRV3C incubated (GE Healthcare) on ice for 60 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... which was added to the membrane for 1 min before the signal was detected using the ImageQuantTM LAS 4000 (GE Healthcare). Antibodies and dilutions used ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then placed in 5% milk PBST plus 1:2500 rabbit specific horseradish peroxidase (HRP) antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were then incubated with SuperSignal West Dura or ProSignal Pico Spray chemiluminescence substrate for 1-4 minutes at room temperature and imaged using an Amersham Imager 600 (GE Healthcare) or ChemiDoc (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... Purified NanR at 10 mg mL-1 (340 μM) was injected (70 μL) onto an inline Superdex S200 5/150 Increase (GE Healthcare), equilibrated with buffer C ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was then cleared by centrifugation (18000 g, 4 °C, 1 hour) and purified using a HisTrap HP 5 ml column (GE Healthcare) and a 10-100 mM imidazole linear gradient followed by elution with 250 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... the buffer was supplemented with 30 mM imidazole and an additional 100 mM NaCl and passed through a 1 ml Hi-Trap Chelating column (GE Healthcare) to capture the N-terminal fragment that was cleaved by the TEV protease ...
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bioRxiv - Biophysics 2022Quote: ... The individual complexes were incubated over ice for 1 hour and purified by size-exclusion chromatography (SEC) using a Superdex 200 10/300 GL column (GE healthcare) pre-equilibrated in a buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... GST-Lc3b was constructed by cloning Lc3b into the BamHI and SalI sites of pGEX-4T-1 (GE Healthcare, IL, USA). The pac sequence was amplified from pBApo-EF1α Pur and cloned into pBApo-EF1α Neo ...
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bioRxiv - Microbiology 2019Quote: ... before the supernatant was applied at 1 ml/min to a 5 ml chromatography column attached to an ÄKTA Prime plus (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... analytical gel size-exclusion chromatography was performed with 1-2 mg protein in buffer A on a Superdex 75 10/300 column (GE Healthcare), to which a static light-scattering (SLS ...
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bioRxiv - Biochemistry 2019Quote: ... Bacterial lysates were collected as described above and the lysates were incubated with 1 ml Glutathione Sepharose 4B (GE Healthcare, UK) at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2019Quote: ... The PCR product was purified and digested with BamH1 and SalI and cloned into in the pGEX-6P-1 vector (GE Healthcare) to generate GST-BRCA2190-283 ...
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bioRxiv - Biochemistry 2020Quote: ... 80 µg of a 5’ amino-modified DNA oligonucleotide complementary to the target tRNA (IDT) was covalently coupled to a HiTrapTM NHS-activated HP column (1 mL, GE Healthcare). For isolation of full-length tRNAs ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid pGEX-dosR expressing DosR with an N-terminal GST-tag was generated by cloning PCR-derived dosR ORF fragment between BamHI and XhoI sites of pGEX 4T-1 (GE Healthcare) as described for pGEX-phoP (49) ...
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bioRxiv - Biochemistry 2019Quote: ... and a 1:2500 dilution of the fluorophore-conjugated secondary antibody Cy3 (ECL Plex goat-α-mouse IgG-Cy3, GE Healthcare). Detection was carried out with the Ettan DIGE Imager (GE Healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... sidI was amplified using primer pairs SidIBamHI-F/SidINotI-F and cloned as a BamHI/NotI fragment into pGEX-6P-1 (GE Healthcare) and pT7HMT (55) ...
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bioRxiv - Biochemistry 2019Quote: ... Cell debris was removed by centrifugation at 10000xg for 45 min at 4°C and the supernatant was loaded onto either a 1 mL or 5 mL HisTrap FF crude column (GE Healthcare) equilibrated in Ni-1 buffer using a peristaltic pump ...
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bioRxiv - Molecular Biology 2019Quote: ... washed 3x with TBS-T and incubated for 1 hours at RT with the secondary antibody (horseradish peroxidase-conjugated sheep anti-mouse or donkey anti-rabbit IgG, GE Healthcare). Membranes were analyzed with a Fusion Solo (Vilber Lourmat) ...