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Citations for GE Life Sciences :
2301 - 2350 of 2615 citations for Progesterone ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... washed with 1% BSA/PBS and incubated with Cy3-conjugated or FITC-conjugated goat anti-rabbit IgG (10 mg/ml; GE Healthcare) for 45 min respectively ...
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bioRxiv - Developmental Biology 2019Quote: ... or 1 ng (for other mRNAs) of mRNAs in 1× modified Barths Solution (MBS) containing 4% Ficoll-400 (17-0300-05, GE Healthcare) using a microinjector (PLI-90 ...
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bioRxiv - Biochemistry 2021Quote: ... purified OxlT was mixed with purified 20D033-Fv at a 1:2 molar ratio at 4 °C overnight and purified using Superdex200 Increase 10/300 GL (GE healthcare) in 20 mM MES-KOH ...
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bioRxiv - Molecular Biology 2021Quote: ... Binding was detected by incubating the plates sequentially with either mouse anti-Histidine Tag antibody (MCA1396, Abd Serotec) followed by horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2000, NXA931, GE Healthcare) or Streptavidin-HRP (554066 ...
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bioRxiv - Molecular Biology 2019Quote: ... and boiled for 5 min at 95°C were separated on 6-8% polyacrylamide/7 M Urea denaturing gel in 1× TBE and transferred onto an Amersham HybondN+ membrane (GE Healthcare). RNA was UV-crosslinked to the membrane ...
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bioRxiv - Biochemistry 2020Quote: ... containing 0.05 M NaCl to a final concentration of 1 mg/ml was applied to a Superdex 10/300 column (GE Healthcare) run at a flow rate of 0.5 ml/min ...
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bioRxiv - Cancer Biology 2021Quote: ... or HRP-conjugated anti-mouse (CST, 7076; 1:1000) IgG secondary antibodies and visualized using ECL Prime Western Blotting Detection Reagent (GE Healthcare) and ImageQuant LAS 4000 Mini (FUJIFILM) ...
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bioRxiv - Biochemistry 2021Quote: The eluent from the Ni-NTA purification of the expression product of the pET-Duet-1 vector in BL21(DE3)pLysS* cells was loaded onto a 24 ml Superdex-200 10/300 GL (GE Healthcare) column on an AKTA Purifier-10 workstation ...
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bioRxiv - Biochemistry 2021Quote: ... and the digestion products were then loaded in 7 M urea containing 20% (wt/vol) polyacrylamide gels (acrylamide/bisacrylamide ratio 19:1) buffered with TBE and visualized using a Fluorescent Image Analyzer Typhoon (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μL of each homogenate was loaded onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA) and left to dry out at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... 15 mM betamercaptoethanol, and 1 mM PMSF) and lysed using a microfludizer (Microfluidics, Inc.) followed by passage over a HisTrap column (GE Healthcare) and elution with a 20-400 mM imidazole gradient in lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of protein sample diluted to a concentration of between 0.02 mg/ml and 0.05 mg/ml was added to a grid and then manually blotted away with Whatman Grade 1 filter Paper (GE Healthcare). 3 µl of 0.75% Uranyl Formate was then added to the grid and blotted away ...
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bioRxiv - Biochemistry 2021Quote: ... Mer2 constructs were eluted with a lysis buffer containing 1 mM maltose and passed through a 6 mL ResourceQ column (GE Healthcare) equilibrated in 50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... the protein solution was again centrifuged at 15000 rpm for 1 hour and was loaded in an anion exchange column (Resource Q, GE healthcare) through an AKTA purifier FPLC system (Cytiva) ...
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bioRxiv - Plant Biology 2020Quote: ... DNA samples digested with restriction enzymes were separated in 1% agarose gels and blotted onto Hybond-XL nylon membranes (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... after which the lysate was pre-cleared for 1 h at 4 °C with 40 μl of equilibrated protein G-Sepharose beads (GE Healthcare). The day before ...
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bioRxiv - Biophysics 2021Quote: ... Purified TolC and colicin E1-T or -TR were mixed at a 1:2 molar ratio to ensure an excess of colicin to saturate TolC binding sites and incubated at room temperature for 1 hour before loading onto a Superdex 200 Increase 10/300 GL column (GE Healthcare). The protein was eluted with 1.5 column volumes into 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: A mixture of 1 µM GST-NDP52 and 100 nM GFP-ULK1 complex was incubated with 10 µL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... of RNA was resolved on a 1% agarose denaturing gel followed by blotting on a nylon membrane (Amersham Hybond-N+, GE Healthcare). A probe for mCherry or for 4× Broccoli was synthesized by PCR using the 4× Broccoli plasmid as template and the primers prW150-prW151 for Broccoli and prW459-prW460 for mCherry ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton X-100) and incubated 1 hour at RT or 16 hours at 4°C with HRP-coupled streptavidin (RPN1231VS, GE Healthcare) diluted 1:20000 in BSA blocking buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μl purified recombinant CPF at 1 mg/ml was loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... Full-length coding regions were amplified and inserted in frame into pET-28a(+) (69864-3, MilliporeSigma) and/or pGEX-4T-1 (28-9545-49, GE Healthcare) to create N-terminal ...
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bioRxiv - Cell Biology 2021Quote: ... and GST-SV40-NLS were cloned by in-frame insertion of the corresponding NFAT5 fragments and SV40-NLS into the C-terminus of GST of pGEX-4T-1 (GE Healthcare), respectively ...
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bioRxiv - Genomics 2021Quote: ... and AsNRDE-3 amino acids 45-246 (HQ611973) were fused in frame with GST using the Bam H1 site of pGEX-6P-1 (GE Healthcare), the proteins were expressed in E ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were cleared for 30 min at 12,500 × g at 4°C and the supernatant was collected and fractionated on a 1 ml Q-HiTrap column (GE Healthcare) equilibrated in 20 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... and exposed on X-ray films (Kodak Biomax MR-1) or visualized using GE ImageQuant LAS4000 camera (GE Healthcare Life Sciences).
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl and 1 mM DTT (survivin SEC buffer) and gel filtration using a Superdex 75 100/300GL column (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... 6 μg of total RNAs were resolved on a 1 % (w/v) agarose gel then transferred onto Amersham Hybond-N+ Membrane (GE Healthcare) by capillary transfer ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl of the homogenate was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA) and left to dry out at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... HIV-1 IN was further fractionated by size exclusion chromatography (SEC) with a Superdex 200 Increase (10/300) column (GE Healthcare) and stored at −80°C (Fig ...
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bioRxiv - Biochemistry 2022Quote: ... The chromatographic eluent was monitored by three consecutive detectors in series: (1) a multi-wavelength UV-Vis absorbance detector Monitor UV-900 of the AKTA system (GE Healthcare) with a 10 mm path length flow cell ...
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bioRxiv - Biochemistry 2022Quote: ... both MSL8N and MSL9N were placed in a high salt storage buffer (1 M NaCl, 200 mM Sodium Phosphate) using gravity PD-10 desalting columns (GE Healthcare). The protein was labeled with Alexa Fluor 647 NHS (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... was diluted to a final salt concentration of 200 mM NaCl before loading onto a 1 mL heparin column (GE Healthcare) equilibrated in low-salt buffer (50 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... was incubated 2 h at a dilution of 1:10,000 and revealed by ECL Prime colorimetric assay (GE Healthcare, Chicago, USA).
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bioRxiv - Biophysics 2022Quote: ... The TOP3A-RMI1/2 complex was assembled by incubating purified TOP3A and RMI1/2 (1:3 ratio) followed by purification using a Superdex 200 Increase (GE Healthcare) in buffer I.
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bioRxiv - Biochemistry 2022Quote: ... and passed through two subsequent gel filtration steps using a: 1) Hiload 16/60 Superdex 75 column (Cytiva #28989333) and 2) and Sephacryl S-300 column (GE Healthcare). Buffers used for this sizing step and subsequent labeling reactions were followed as for full-length proteins ...
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bioRxiv - Biochemistry 2022Quote: ... The second PCR joined these two spliced fragments to generate the PCR product encoding the H172Y mutated Mpro including the cleavage sites of the restriction enzymes for cloning into the vector PGEX-6p-1 (GE Healthcare). The amplified PCR product was digested with BamHI and XhoI and ligated into the vector PGEX-6p-1 digested with the same restriction enzymes ...
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bioRxiv - Cell Biology 2022Quote: ... were acquired every 1–3 min over a total time period of 4–6 h and deconvolved using the softWoRx software (Applied Precision). In addition ...
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bioRxiv - Cell Biology 2022Quote: ... either wild-type (termed GSTscc2PIP) or with PIP mutated (Scc2-N1479A,L1482A,Y1485A,F1486A, termed GSTscc2pip) was introduced into pGEX-6P-1 (GE Healthcare) vector replacing the last 18 residues of GST ...
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bioRxiv - Genetics 2022Quote: ... Products of were separated using 6% denaturing sequencing gel for 1 hr at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... and bands visualized with (HRP)- coupled secondary antibody (1:5,000) (Cell Signalling, rabbit 7074S, mouse 7076S) on an ImageQuant LAS 4000 Mini (GE Life Sciences) with Luminata Forte Western HRP Substrate (Sigma WBLUF0500 ...
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bioRxiv - Molecular Biology 2022Quote: ... validated by genotyping PCR that was analyzed on a 1% agarose gel imaged with a Typhoon FLA 9500 scanner (GE Healthcare). Subsequent Sanger sequencing of the PCR product (Microsynth ...
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bioRxiv - Microbiology 2022Quote: ... of the extension reaction were separated by electrophoresis on a denaturing PAGE (8 M urea, 1× TBE, 15 % acrylamide) and then imaged (Typhoon 9500 imager; GE Healthcare) and the images were quantified using Image Lab software (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The lysates were clarified by centrifugation at 20 000 × g for 1 hour at 4 °C before loading on a 5 mL HiTrap FF Ni-NTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Plant Biology 2022Quote: ... purified PIN1 and sybody-21 were mixed together with a molar ratio of ∼1:3 and then applied to size-exclusion chromatography using a Superose 6 Increase column (GE Healthcare) in buffer containing 25 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Immunology 2022Quote: ... to 3 mL for loading onto a 1 × 66 cm gel filtration column comprised of 4 g of degassed Sephadex G-50 beads (GE Healthcare) preequilibrated in buffer A O/N ...
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...