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Citations for GE Life Sciences :
2051 - 2100 of 4168 citations for 7 Oxa 1 thia 3 azaspiro 4.4 non 2 ene 4 6 dione 2 amino 8 8 dimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Cleaved topo VI fractions were pooled and concentrated using Amicon Ultra 15 mL centrifugal filter units (30 kDa cut off, MilliporeSigma) before being passed down a Superose 6 10/300 (GE Life Sciences) column in Buffer C (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting pellet was resuspended in TET and complexes were further separated by Superose 6 10/300 column chromatography in TET buffer with 150 mM NaCl using an AKTA Pure system (GE Life Sciences). The sample collected from the column was used for microscopy ...
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bioRxiv - Biochemistry 2019Quote: ... the reaction was quenched with 10 mM DTT and subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 (GE Healthcare) in GF buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction was stopped with 1.7 mM Tris-HCl pH 8.0 and crosslinked TcdA1-BSA-Lewis X was purified from excess of BSA-Lewis X by SEC using a Superose 6 3.2-300 column (GE Healthcare Life Sciences).
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bioRxiv - Genomics 2021Quote: ... Barcoded beads were resuspended in lysis buffer (200 mM Tris-HCl pH8.0, 20 mM EDTA, 6% Ficoll PM-400 (GE Healthcare/Fisher Scientific), 0.2% Sarkosyl (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... The dried butanol fraction was then redissolved in 20mM of sodium phosphate buffer (pH 6) and desalted using a NAP10 desalting column prepacked with Sephadex G-25 resin (GE healthcare, USA). Finally ...
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bioRxiv - Microbiology 2020Quote: ... The protein (5-6 ml) was further purified by FPLC over a 26/60 Superdex 200 pg size exclusion column (GE Life Sciences) equilibrated with 20 mM Tris (pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... Complex samples were either diluted to 0.02 mg/mL and applied to glow discharged negative stain grids or they were SEC purified using a Superose™ 6 Increase 10/300 GL (GE Healthcare) column to remove excess Fab prior to EM grid preparation ...
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bioRxiv - Genetics 2020Quote: ... the eluted protein solution was concentrated 10 times in a Vivaspin concentrator 30 kDa cut off and loaded in a Superose 6 increase 10/300 (GE Life Sciences) with PBS as a carrier buffer ...
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bioRxiv - Biochemistry 2021Quote: ... filtered (0.22 mm) and then applied to 5 mL HiTrap Chelating HP columns (GE Healthcare, protein from 6 L cells per column) charged with Ni2+ and equilibrated with 4 column volumes of buffer C (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: Secreted candidate proteins were purified by immobilized metal ion affinity (IMAC) using Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL, EUA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The eluent was concentrated and further purified by using a size-exclusion chromatography (SEC) column (Superose 6 increase 10/300 GL, GE Healthcare, USA) in 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... The samples (equivalent to ∼85 µg microsomes) were then analyzed with an HPLC system and injected to a Superose 6 column (GE Life Sciences), equilibrated in a running buffer containing 25 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2021Quote: The protein was purified from filtered cell supernatants using Strep-Tactin resin (IBA) before being subjected to additional purification by gel filtration chromatography using a Superose 6 10/300 column (GE Healthcare, USA) in 1 × PBS ...
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bioRxiv - Biophysics 2022Quote: ... Sample homogeneity was investigated by analytical size exclusion chromatography on a Superose® 6 10/300 GL gel filtration column (GE Healthcare). Aliquots of purified EFL at 0.4 mg/mL were flash frozen in liquid nitrogen and stored at −70 °C.
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bioRxiv - Microbiology 2022Quote: ... The leukocyte-depleted red blood cell pellet was then resuspended in 10 ml PBS and 5 ml were carefully layered on 6 ml of 70% (v/v) isotonic Percoll cushion (GE Healthcare, UK). After centrifugation at 1200 x g for 15 mins ...
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bioRxiv - Genetics 2024Quote: ... Barcoded beads were resuspended in lysis buffer (200 mM Tris-HCl pH8.0, 20 mM EDTA, 6% Ficoll PM-400 (GE Healthcare/Fisher Scientific), 0.2% Sarkosyl (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: SEC-MALS experiments were performed using and Ä KTA purifier system connected to a Superose 6 Increase 10/300 GL column (GE healthcare) at a flow rate of 0.4 ml/min in running buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: Purified LZTR1 proteins were centrifuged at 12,000×g for 10 min before being applied to an analytical Superose 6 10/300 SEC column (GE Healthcare Life Sciences) using a buffer containing 50 mM Tris/HCl (pH 7.4) ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting ABC holotoxins were then separated from excess TcB-TcC by size exclusion chromatography on a Superose 6 increase 5/150 column (GE Life Sciences) equilibrated in labelling buffer ...
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bioRxiv - Genetics 2023Quote: ... GST-Clr4 and MBP-Clr6 were expressed in Escherichia coli BL21 (DE3) cells and purified on Ni+ SepharoseTM 6 Fast Flow (for 6His fusions; GE Healthcare, 17531806), Glutathione SepharoseTM 4B (for GST fusions ...
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bioRxiv - Biophysics 2022Quote: ... The concentrated protein sample was subjected to further size-exclusion chromatography (SEC) by a Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare, USA) using a flow rate of 0.3 mL/min and a running buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Physiology 2023Quote: Plasma samples (200 μL) were loaded onto AKTA pure FPLC column [Superose™ 6 Increase 10/300 GL FPLC column (GE Healthcare). Lipoproteins were eluted with PBS and 0.25 mL fractions were collected at a flow rate of 0.4 mL/min.
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bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
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bioRxiv - Biochemistry 2024Quote: Oligomerization of ClpL WT and mutants was investigated by size exclusion chromatography (SEC) using a Superose® 6 10/300 GL column (GE Healthcare) at 4°C in assay buffer supplemented with 2 mM ATP ...
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bioRxiv - Genomics 2020Quote: ... Membranes were washed 3 times in TBST and incubated with Amersham ECL PLUS™ (GE Healthcare, RPN2132) prior to exposure to Super RX film (Fujifilm ...
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bioRxiv - Immunology 2019Quote: ... Columns were prepared by coupling Y-3 and B22-249 antibodies to CNBr-activated Sepharose (GE Healthcare) (1 mg antibody/40 mg Sepharose) ...
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bioRxiv - Microbiology 2021Quote: Recombinant CNF1 toxin was fluorescently labeled with Cy3 (Cy®3 Mono 5-pack, GE healthcare, PA23001) according to the vendor’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: The study was performed using a 3-Tesla scanner (HDx Signa platform, GE healthcare, Milwaukee, WI, USA) with an 8-channel head coil ...
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bioRxiv - Neuroscience 2020Quote: ... Binding was detected with an anti-mouse Cy-3 and anti-rabbit Cy-5 antibodies (GE Healthcare). Blots were scanned using a Typhoon 5 (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: The study was performed using a 3-Tesla scanner (HDx Signa platform, GE healthcare, Milwaukee, WI, USA) with an 8-channel head coil ...
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bioRxiv - Immunology 2020Quote: ... Post-nuclear supernatants were incubated with 3 μg Protein A Sepharose CL-4B (PAS; Ge HealthCare, #GEH17078001) for 1 hour and then with 3 μg PAS plus primary antibody (0.4 ug anti-GFP mAb ...
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bioRxiv - Neuroscience 2024Quote: The experiments were performed in a 3-Tesla scanner (HDx Signa platform, GE healthcare, Milwaukee, WI, USA) with an 8-channel head coil ...
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bioRxiv - Neuroscience 2023Quote: MRI scans were performed using a GE 3 Tesla MR750 Discovery scanner (GE Healthcare, Milwaukee WI, USA). The anatomical scan acquired a T1-weighted image with the MPRAGE sequence of TR/TE = 5/2 ms ...
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bioRxiv - Neuroscience 2023Quote: ... (3) Size exclusion chromatography (SEC): EVs were isolated from ISF using Sephadex-G25 (GE Healthcare; Uppsala, Sweden) following the vendor’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... the cDNA of GmPHR1 was cloned into the prokaryotic expression vector pGEX-6p-3(GE Healthcare, USA) cleaved with BamHI ...
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bioRxiv - Developmental Biology 2020Quote: ... The fusion proteins were affinity-purified on Glutathione Sepharose® 4 Fast Flow (GE Healthcare), and then bound to POROS GoPure XS (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was added to a slurry of Glutathione Sepharose 4 Fast Flow (GE Healthcare) and incubated for 2 h at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... the clarified supernatants were loaded in 4 mL of Ni2+-sepharose beads (GE Healthcare, Uppsala), washed with Binding Buffer (4 × 4 mL ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C) and the supernatant was applied to a 5 ml HiTrap column (GE Healthcare) loaded with cobalt(II)-chloride hexahydrate (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C) and the supernatant was applied to a 5 ml HiTrap column (GE Healthcare) loaded with cobalt(II)-chloride hexahydrate (Sigma#255599) ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein-G Sepharose 4 Fast Flow and SAv-Sepharose beads were obtained from GE Healthcare Bio-Sciences AB ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was precleared with beads (Protein G sepharose™ 4 flast flow, GE Healthcare) for 1 hour at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were precleared with Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 30 min at 4 °C under rotation ...
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bioRxiv - Plant Biology 2021Quote: ... coli strain BL21 (DE3) and purified with Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) by following the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... Synaptosomes were purified from 4-5 cerebella on discontinuous Percoll gradients (GE Healthcare, Uppsala, Sweden). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... and a multi-frequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare, USA) was utilized to capture a VL image the transverse plane at approximately 50% the distance between the mid-inguinal crease and the proximal patella ...
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bioRxiv - Biochemistry 2022Quote: ITC measurements were performed at 4 °C using a MicroCal auto-iTC200 calorimeter (GE Healthcare). Samples were extensively dialyzed against a buffer containing 500 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... A second incubation overnight at 4°C with Protein G-magnetic Sepharose beads (GE Healthcare) was used to pull down protein:DNA complexes prior to washing in low salt (120mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... NHS-activated Sepharose 4 Fast Flow and Q-Sepharose Fast Flow was from GE Healthcare Life Sciences ...