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Citations for GE Life Sciences :
1951 - 2000 of 2758 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from the cells using Illustra RNAspin Mini Kit (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... cells were resuspended in 30% Percoll (100% Percoll Plus, 17-5445-02, GE Healthcare) and centrifuged at 300g for 15 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The cell culture medium was MEM/F-12 mixture (HyClone; GE Healthcare Life Sciences) containing 10% fetal bovine serum (HyClone ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... High-throughput imaging was carried out using the In Cell Analyzer 2000 (GE Healthcare). Cell viability was quantified by using the propidium iodide signal to count the total number of cells per well and using the DAPI signal to count the total number of dead cells per well in the GE Developer Toolbox v1.9.1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... High-throughput imaging was carried out using the In Cell Analyzer 2000 (GE Healthcare). Cell viability was quantified by using the propidium iodide signal to count the total number of cells per well and using the DAPI signal to count the total number of dead cells per well in the GE Developer Toolbox v1.9.1 ...
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bioRxiv - Bioengineering 2023Quote: ... Cell cultures were controlled using the UNICORN™ system control software (GE Healthcare, USA). Cells were seeded at ∼ 7.93 × 108 total viable cells (around 7.93 × 105 viable cells/mL ...
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bioRxiv - Microbiology 2022Quote: ... and peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll density centrifugation (GE HealthCare). PBMCs were counted and viably cryopreserved in liquid nitrogen vapor (10% DMSO ...
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bioRxiv - Microbiology 2023Quote: ... The cell lysate was loaded on a Ni-sepharose FF HiLoad column (GE Healthcare), equilibrated in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... The resulting cell suspension was resuspended in 40% Percoll (GE Healthcare Biosciences, Uppsala, Sweden) in DMEM and layered on 70% Percoll in DMEM ...
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bioRxiv - Biochemistry 2023Quote: ... and THP-1 g2c2 cells were cultured in Iscove’s modified Dulbecco’s medium (GE Healthcare) supplemented with 10% fetal bovine serum and penicillin/streptomycin (100 U/mL and 100μg/mL ...
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bioRxiv - Biochemistry 2023Quote: Cell free extract was applied to a 5 ml HisTrap FF column (GE Healthcare), and unbound proteins were washed off with 10 column volumes of Buffer HisA (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... coli or insect cells as fusion proteins subcloned into pGEX-6P-1(GE Healthcare) or te pFastBac-1 vector (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were resuspended in 10 ml of 40% Percoll (GE Healthcare, Chicago, IL) and centrifuged again at 950 x g for 20 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysate was then loaded onto glutathione SepharoseTM 4 Fast Flow resin (GE Healthcare), and washed with Tris buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Image acquisition was done using an IN Cell Analyzer 6000 (GE Healthcare Life Sciences). Each well was imaged in a 9 x 9 grid at 20X magnification ...
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bioRxiv - Biochemistry 2023Quote: ... the cell lysates in buffer D was incubated with Ni-NTA resin (GE Healthcare), washed using buffer D supplemented with 20 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... Abs were purified from the transfected cell supernatants with rProtein A-Sepharose (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... pombe cells were observed using a DeltaVision Elite system (GE Healthcare Inc., Chicago, USA) equipped with a pco.edge 4.2 sCMOS (PCO ...
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bioRxiv - Molecular Biology 2024Quote: ... coli cell fluorescence was carried out using a Typhoon FLA 9500 fluorimager (GE Healthcare) as described in Olorunniji et al ...
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bioRxiv - Biochemistry 2024Quote: ... The cleared cell lysate was loaded on a 5 mL MBPTrap column (GE Healthcare). The captured proteins were washed with 5 CV wash buffer (20 mM HEPES pH 8 ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed with PBST and incubated with alkaline phosphatase-conjugated secondary antibodies (anti-rabbit IgG and anti-mouse IgG, GE healthcare life sciences, 1:10000) for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Western blotting was performed as previously described using Horseradish Peroxidase (HRP)-conjugated secondary antibody followed by addition of ECL (GE Healthcare Bio-Sciences Corp., USA) to reveal chemiluminescence71 ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated for 90 minutes at room temperature with secondary antibodies (donkey anti-rabbit HRP-IgG, GE Healthacare, cat#NA934V, 1:5000 or goat anti-mouse HRP-IgG, GE Healthcare, cat#NA931V, 1:2000) diluted in blocking solution and washed as before ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Membranes were washed and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and detected by chemiluminescence system (GE Healthcare Europe GmbH, Freiburg, Germany).
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bioRxiv - Biophysics 2019Quote: ... The membrane was rinsed three more times in TBS-Tw and incubated for 30 min with secondary antibody (HRP-conjugated sheep anti-mouse IgG, GE Healthcare Life Sciences; Freiburg, Germany) diluted 1:20,000 in TBS-Tw + 1% milk ...
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bioRxiv - Immunology 2019Quote: ... IgG and IgM antibodies were purified from plasma samples by affinity chromatography using prepacked HiTrap™ Protein G HP columns (GE Healthcare, Wauwatosa, WI, USA) and HiTrap™ IgM Purification HP columns (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Physiology 2020Quote: ... Immunoblotting was conducted with specific antibodies and protein bands were visualized using the enhanced chemiluminescence (ECL) Western Blotting Detection System (GE Healthcare, Chalfont St Giles, UK) or the Odyssey infrared detection system (LICOR).
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bioRxiv - Microbiology 2020Quote: ... IgG and IgA antibodies were depleted from plasma obtained from 25 recovered COVID-19 patient using Protein G HP Spintrap (GE Healthcare Life Sciences, Buckinghamshire, UK) and Peptide M / Agarose (InvivoGen ...
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bioRxiv - Immunology 2020Quote: ... and class II molecules were isolated by standard immunoaffinity purification using purified monoclonal antibody 2G11 [65] produced in-house and covalently linked to CNBr-activated Sepharose (GE Healthcare, Chalfont St Giles, UK). MHC–peptide complexes were eluted by repeated addition of 0.2% trifluoroacetic acid (TFA ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by an incubation with horseradish peroxidase (HRP) secondary antibodies (Mouse IgG HRP Linked Whole Ab-GE Healthcare, #NA931, 1:5000; Rabbit IgG HRP Linked Whole Ab-GE Healthcare, #NA934, 1:5000) in 3% milk in TBST ...
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bioRxiv - Cancer Biology 2022Quote: 1 mg lysate protein aliquots were used for immunoprecipitation with optimized amounts of the indicated antibodies and 20 μL of Protein A Sepharose beads (GE Healthcare, Chalfont St. Giles, UK) followed by SDS-Polyacrylamide (Biorad ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed with TBS-T and probed with the species-matching secondary horseradish peroxidase-conjugated antibody: donkey anti-rabbit (GE Healthcare, cat. #NA932, dilution 1:10,000), or sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were washed three times with TBST for 10 minutes each and exposed to donkey anti-rabbit secondary antibody conjugated to horse radish peroxidase (GE Healthcare; NA934V; LOT: 16976257; 1:10,000) in TBST containing 5% (w/v ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked and incubated with the antibody to detect neural Wiskott-Aldrich Syndrome protein (N-WASp; MilliporeSigma, Burlington, MA, USA) using horseradish peroxidase-linked secondary antibodies and an Enhanced Chemiluminescence System (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Autoradiography was performed using UltraCruz Autoradiography Films (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2019Quote: All fixed cell images were acquired using a DeltaVision Personal DV Imaging system (GE Healthcare) on an IX71 inverted microscope (Olympus ...
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bioRxiv - Cell Biology 2020Quote: ... live cell imaging was performed using a DeltaVision Elite imaging system (GE Healthcare, Pittsburg, PA). The DeltaVision Elite is comprised of a wide field inverted epifluorescence microscope (1×71 ...
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bioRxiv - Cell Biology 2020Quote: Live-cell fluorescence microscopy was conducted on a DeltaVision imaging system (GE Healthcare Life Sciences) with a 63× objective lens (NA=1.40 ...
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bioRxiv - Microbiology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque, GE Healthcare) from buffy-coat preparations of blood from healthy donors ...
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bioRxiv - Immunology 2021Quote: The prepared cell supernatants and sera were first filtered through 1-μm filters (GE Healthcare Whatman Puradisc GMF ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were grown for 26-30 hours before scanning on a Typhoon FLA9500 (GE Healthcare) fluorescence scanner equipped with 488 nm and 532 nm excitation lasers and 520/40 and 610/30 emission filters ...
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bioRxiv - Neuroscience 2022Quote: ... Mononuclear cells were separated from myelin via centrifugation in a 27% Percoll solution (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: ... the cell culture medium was loaded onto a HiTrap MabSelect 5 mL column (GE Healthcare), using an ÄKTA Pure 25 (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by centrifugation using Ficoll-Paque PLUS (GE Healthcare). PBMCs were resuspended in R10 media consisting of RPMI-1640 (Wisent) ...
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bioRxiv - Genetics 2019Quote: ... Fluorescent images of cells were collected on the INCell Analyzer 2000 automated microscope (GE Healthcare) (10X objective ...
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bioRxiv - Biochemistry 2020Quote: ... we immobilised PTEN on flow-cell (FC) 2 of a CM4 sensor chip (GE Healthcare) using amine coupling (NHS/EDC) ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (200 μg) were incubated with 100 μL slurry of Streptavidin beads (GE Healthcare) for 1 h at RT with end-over-end mixing ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclei were stained with DAP1 and cells were imaged on the DeltaVision (GE Life Sciences) microscope ...
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bioRxiv - Cancer Biology 2019Quote: ... Leukemic cells were isolated by ficoll density gradient using Ficoll-Paque™ Premium (GE Healthcare) and stored in liquid nitrogen ...