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Citations for GE Life Sciences :
1951 - 2000 of 2507 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... CezanneCAT was eluted by cleavage of the GST tag with 15 units/ml TEV protease (SigmaT4455-1KU) in PBS buffer overnight at 4°C and desalted using a PD-10 column (GE Healthcare, cat. # 17085101) in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Synthetic Biology 2024Quote: The OVA protein was first attached to an oligonucleotide using a succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) linker and purified using a NAP column (GE Healthcare Life Sciences). Unconjugated oligonucleotides were removed by 30K Amicon filtration (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... each studied sample—the entire EC and entire MB prepared from each hemi-forebrain—was incubated with 40 µL of Protein G Sepharose 4 Fast Flow resin (GE healthcare, Piscataway, NJ) slurry (volume of settled beads:volume of storage buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... 50 µL of 6 µM Cdt1 or Cdt1Uniform was prepared in Protein Buffer and then loaded and run on a Superose 5/150 GL sizing column (GE Healthcare) pre-equilibrated and run in Protein Buffer ...
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bioRxiv - Biophysics 2021Quote: ... the supernatant was filtered using a 0.45 µm filter and then loaded onto a 5 ml HisTrap™ HP nickel sepharose column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Biophysics 2021Quote: ... suspended in buffer QA (10 mM Tris pH 8 and 1 mM EDTA) and loaded into a MonoQ 5/50 GL column (GE Healthcare). After a step-wise wash with 10% and 20% of buffer QB (Buffer QA supplemented with 2M NaCl) ...
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bioRxiv - Cell Biology 2020Quote: GST-tagged proteins (Retromer, SNX3, WASHC2C-5 and WASHC2C-21) were purified by incubating clarified lysate with glutathione (GSH) Sepharose 4B (GE Healthcare) beads ...
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bioRxiv - Cell Biology 2020Quote: ... 250 μg of MCF10A cell lysate and 5 μg of individual GST-tagged recombinant proteins were mixed and incubated with glutathione Sepharose 4b beads (GE Healthcare) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 or 10 µg of the purified RNA was resolved on formaldehyde agarose gels and transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the dialyzed sample was loaded on an anion exchange HiTrap Q HP 5 mL column (GE healthcare life sciences, Boston, Massachusetts) pre-equilibrated with 20 mM bis-Tris pH 5.5 ...
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bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then placed in 5% milk PBST plus 1:2500 rabbit specific horseradish peroxidase (HRP) antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified NanR at 10 mg mL-1 (340 μM) was injected (70 μL) onto an inline Superdex S200 5/150 Increase (GE Healthcare), equilibrated with buffer C ...
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bioRxiv - Biophysics 2022Quote: All samples were pre-reduced with 5 mM DTT for one hour followed by desalting using a PD10 column (GE Healthcare) into 20 mM tris pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... Cell disruption was achieved by sonication (Branson Sonifier SFX250) and the soluble fraction was subjected to immobilized metal affinity purification using a 5 ml Ni-NTA column (GE healthcare) connected to a FPLC system (GE healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... The eluted fractions were concentrated to 5 ml and loaded onto a HiLoad 26/600 Superdex 200 prep grade (GE Healthcare) equilibrated with the SEC buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... 1.5 μM PCNA trimer and 20 μM TTP was loaded onto a Superdex 200 increase 3.2/300 column (GE Life Sciences) equilibrated with a buffer comprising [25 mM HEPES (pH 7.5) ...
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bioRxiv - Bioengineering 2020Quote: ... The homogenate was centrifuged as before and the supernatant was purified on an AKTA Pure FPLC equipped with a HiTrap Q 5 mL anion exchange column (GE Healthcare). Solvent A was 20 mM tris pH 8.5 ...
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bioRxiv - Bioengineering 2020Quote: ... and the supernatant was purified using an AKTA Pure FPLC equipped with a HisTrap FF crude 5 mL column (GE Healthcare). Solvent A was 20 mM tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Tag and TEV protease removal was achieved by re-loading the dialyzed sample onto the 5 mL HisTrap Excel column (GE Healthcare), and collecting the unbound tagless sample ...
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bioRxiv - Biochemistry 2020Quote: ... The protein of interest (NT-mini or NT*-mini) was then eluted into a 5 ml Ni-IMAC HisTrap Excel column (GE Healthcare) by further increasing the NaCl concentration to 600 mM (buffer NT.B) ...
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bioRxiv - Molecular Biology 2020Quote: Wild type or ΔN20 hMcm10-ID (200 µL at 5 mg ml-1) was injected into Superdex 75 Increase 10/300 GL column (GE Healthcare) operating with the elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at -80°C until use ...
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bioRxiv - Biophysics 2020Quote: ... centrifuged at 14000g and 4 °C for 5 min and the excess of ammonium sulfate was removed by running the supernatant through a NAP-25 column (GE Healthcare). The tAv was purified using HiTrap chelating column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: Fifty milliliters of the supernatant was loaded onto a 50 mL superloop (Amersham Biosciences) and bound to a 5 mL heparin column (HiTrap Heparin HP 1X5 mL, GE Healthcare) by fast protein liquid chromatography (ÄKTApurifier ...
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bioRxiv - Biochemistry 2020Quote: ... The cleaved protein was concentrated to 5 ml and loaded in a HiLoad 16/600 Superdex 75pg gel-filtration column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Cleaved protein was passed through a 5 ml HisTrapFF column and further purified by size-exclusion chromatography (S200 16/60 column; GE Healthcare) in buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Soluble RNA was separated from solid acrylamide by filtration through 0.22 micron filters and then manually applied to a 5 mL HiTrapQ column (GE Healthcare # 29051325) that had been equilibrated in buffer D (300 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were clarified by centrifugation (20 minutes at 18,000x g) and bound to a 5 mL HisTrap™ FF Crude column (GE Healthcare) and eluted with 50-75 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... 4°C for 30 minutes and loaded onto a 5 ml HighTrap MBP column (GE Healtcare) using an Äkta-Pure system (GE Healthcare). The column was washed with lysis buffer and protein eluted with 10 column volumes MBP-elution buffer (100 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biophysics 2021Quote: ... The dialyzed media was filtered again and 5 mM imidazole was added to the media before loading onto a Histrap column (GE healthcare). The protein was eluted using a linear imidazole gradient (20 to 500mM ...
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bioRxiv - Molecular Biology 2020Quote: ... Pyo S8 DNase domain was separated from its cognate immunity protein by loading the sample onto a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated with denaturing buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleaved KIX was purified using a linear gradient of 50 mM to 1 M NaCl over ten column volumes by cation exchange chromatography using MonoS 5/50 GL column at a flow rate of 1 mL per minute (GE Healthcare). Fractions containing purified KIX were confirmed by SDS-PAGE and dialyzed against 1 L of 50 mM potassium phosphate pH 5.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... Stained plates were washed 3 times in dH2O for 5 minutes at room temperature and air dried before imaging using the Imagescanner III (GE Healthcare). Growth was determined by percentage area of the plate covered by cells using ImageJ v1.52a ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was next loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was then loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation (40000 g for 30 min) and the supernatant applied onto a 5 ml StrepTrap column (GE Healthcare). After washing with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: The oligomeric state of proteins as well as protein-protein and protein-tRNA interactions were analyzed by loading 50 μl samples on a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate using a Varian ProStar 335 system (Varian Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated in a vivaspin 100,000 MWCO and loaded onto a Superose 6 5/150 size exclusion chromatography column (GE Healthcare Lifescience) to remove thrombin and exchange the buffer to either 20 mm ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 mM EDTA) before further purification by ion-exchange on a MonoQ 5/50 Gl column (GE Healthcare Pte Ltd Singapore) to remove traces of vector and recombination products ...
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bioRxiv - Plant Biology 2020Quote: ... Each Z-section image is based on 15 images generated from 3 different angles and 5 different SIM patterns and reconstructed using SOFTWORX software (Applied Precision).
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bioRxiv - Immunology 2020Quote: ... cells were fed with 5% of the starting volume with Cell Boost7a and 0.5% Cell Boost7b (Hyclone GE Healthcare Life Sciences). Cell counts and glucose was measured as previously described,70 On day 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... divided into aliquots of 10 mL and each aliquot was added on top of a 5 mL of Ficoll-Paque Premium (GE Healthcare) in 15 mL sterile Falcon tubes ...
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bioRxiv - Microbiology 2020Quote: ... VHH containing fractions were pooled and concentrated and the elution buffer was exchanged with PBS with a Vivaspin column (5 kDa cutoff, GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The reaction mixture containing the protein–DNA complex were resolved on a 5% native PAGE gel on ice and transferred to a nylon membrane (Amersham Hybond-XL, GE healthcare) using 0.5 X TBE ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK-293T (ATCC CRL-3216) cell lines used in this study were cultured at 37°C in 5% CO2 in high-glucose DMEM (GE Healthcare) supplemented with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were exposed for 10-30 s (in the case of GFP-Plk4) and for 3–5 min (in the case of actin) on a Amersham Imager 680 (GE Healthcare) and images were acquired with its CCD camera.
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bioRxiv - Biophysics 2021Quote: ... The suspension was then centrifuged at 37,500 g for 30 min and the supernatant incubated with 5 mL GFP nanobody-coupled CNBr-activated Sepharose resin (GE Healthcare) for 2 hrs (Kubala et al. ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM NaCl and 10 mM β-ME and loaded onto a 5 ml HiTrap SP anion exchange column (GE Healthcare). The binary complex ScNatC was then eluted using a salt gradient (50–750 mM NaCl) ...